scholarly journals Waveguide-based surface-enhanced Raman spectroscopy detection of protease activity using non-natural aromatic amino acids

2020 ◽  
Vol 11 (8) ◽  
pp. 4800
Author(s):  
Nina Turk ◽  
Ali Raza ◽  
Pieter Wuytens ◽  
Hans Demol ◽  
Michiel Van Daele ◽  
...  
Keyword(s):  
Amino Acids ◽  
Raman Spectroscopy ◽  
Protease Activity ◽  
Aromatic Amino Acids ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Towards SERS-based multiplexed monitoring of protease activity using non-natural aromatic amino acids

2020 ◽  
Vol 238 ◽  
pp. 04001
Author(s):  
Nina Turk ◽  
Hans Demol ◽  
Andre Skirtach ◽  
Roel Baets ◽  
Kris Gevaert
Keyword(s):  
Amino Acids ◽  
Protease Activity ◽  
Aromatic Amino Acids ◽  
Region Of Interest ◽  
Surface Enhanced Raman Spectroscopy ◽  
Detection Sensitivity ◽  
Peptide Substrates ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Specific Peptide

Surface Enhanced Raman Spectroscopy (SERS) allows sensitive and selective detection of protease activity by monitoring the cleavage of specific peptide substrates. Furthermore, it offers the possibility for multiplexing, during which the activity of two (or more) proteases with different specificities is detected simultaneously. To distinguish between the contributions of different proteases, different aromatic amino acids with non-overlapping SERS peaks need to be used as Raman reporters. As the three natural aromatic amino acids only offer limited possibilities for multiplexing, we examined several non-natural aromatic amino acids with the aim of expanding multiplexing possibilities. We recorded their SERS spectra for the Raman shifts of 300-1700 cm–1 and identified their characteristic SERS peaks. Of the examined nonnatural aromatic amino acids, 3-nitro-tyrosine and two phenylalanines containing stable heavy isotopes seem particularly promising for multiplexing applications. Besides exhibiting characteristic SERS peaks in the spectral region of interest, these non-natural aromatics provide strong SERS peaks compared to natural aromatic amino acids, consequently improving detection sensitivity.

scholarly journals SERS study on myeloperoxidase and its immunocomplex: Identification of binding interactions

2010 ◽  
Vol 24 (3-4) ◽  
pp. 183-190
Author(s):  
Elisabeth S. Papazoglou ◽  
Sundar Babu ◽  
David R. Hansberry ◽  
Sakya Mohapatra ◽  
Chirag Patel
Keyword(s):  
Amino Acids ◽  
Raman Spectroscopy ◽  
Conformational Changes ◽  
Surface Enhanced Raman Spectroscopy ◽  
Biological Molecules ◽  
Sers Spectrum ◽  
Amino Acid Residues ◽  
Igg Antibodies ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Surface Enhanced Raman Spectroscopy (SERS) has demonstrated significant benefit in the identification of biological molecules. In this paper we have examined how to identify and differentiate the 150 kDa protein myeloperoxidase (MPO) from its corresponding antibody (Ab) and their immunocomplex through the use of SERS. The SERS signal of these biological molecules was enabled by 40 nm gold nanoparticles. The SERS spectra for both MPO and the Ab (an IgG molecule) demonstrated results consistent with previous published work on the Raman spectra of MPO and IgG antibodies. The immunocomplex SERS spectra showed peak shifts and intensity variations that could be attributed to conformational changes that occur during immunocomplex formation. Several key spectral areas have been identified which correspond to specific amino acids being shielded from undergoing resonance while new amino acid residues are made visible in the SERS spectrum of the immunocomplex and could be a result of conformational binding. These results indicate that SERS can be used to identify binding events and distinguish an immunocomplex from its individual components.

NIR surface enhanced Raman spectroscopy and bands assignment by DFT calculations of non-natural β-amino acids

2005 ◽  
Vol 310 (1-3) ◽  
pp. 189-199 ◽  
Author(s):  
T. Iliescu ◽  
D. Maniu ◽  
V. Chis ◽  
F.D. Irimie ◽  
Cs. Paizs ◽  
...  
Keyword(s):  
Amino Acids ◽  
Raman Spectroscopy ◽  
Dft Calculations ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Stable Ag@Oxides Nanoplates for Surface-Enhanced Raman Spectroscopy of Amino Acids

2014 ◽  
Vol 6 (11) ◽  
pp. 8853-8858 ◽  
Author(s):  
Peng Du ◽  
Lan Ma ◽  
Yinghui Cao ◽  
Di Li ◽  
Zhenyu Liu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman
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