scholarly journals In vivo photothermal optical coherence tomography of gold nanorod contrast agents

2012 ◽  
Vol 3 (11) ◽  
pp. 2881 ◽  
Author(s):  
J. M. Tucker-Schwartz ◽  
T. A. Meyer ◽  
C. A. Patil ◽  
C. L. Duvall ◽  
M. C. Skala
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Gold Nanorod ◽  
Optical Coherence

In vivo imaging of gold nanorod contrast agents using photothermal optical coherence tomography

2013 ◽  
Author(s):  
Jason M. Tucker-Schwartz ◽  
Travis A. Meyer ◽  
Chetan A. Patil ◽  
Craig L. Duvall ◽  
Melissa C. Skala
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Gold Nanorod ◽  
Optical Coherence

Gold Nanobipyramids as Second Near Infrared Optical Coherence Tomography Contrast Agents for in Vivo Multiplexing Studies

Nano Letters ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Peng Si ◽  
Saba Shevidi ◽  
Edwin Yuan ◽  
Ke Yuan ◽  
Ziv Lautman ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Near Infrared ◽  
Optical Coherence ◽  
Gold Nanobipyramids

scholarly journals In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Maryse Lapierre-Landry ◽  
Andrew Y. Gordon ◽  
John S. Penn ◽  
Melissa C. Skala
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Optical Coherence

scholarly journals A Spectral Demixing Model for Triplex In Vivo Imaging of Optical Coherence Tomography Contrast Agents

ACS Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 893-900
Author(s):  
Edwin Yuan ◽  
Peng Si ◽  
Yonatan Winetraub ◽  
Saba Shevidi ◽  
Adam de la Zerda
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Optical Coherence

scholarly journals Biomolecular Contrast Agents for Optical Coherence Tomography

2019 ◽  
Author(s):  
George J. Lu ◽  
Li-dek Chou ◽  
Dina Malounda ◽  
Amit K. Patel ◽  
Derek S. Welsbie ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Fluorescent Protein ◽  
Mouse Retina ◽  
Optical Coherence ◽  
Gas Vesicles ◽  
Cellular Functions ◽  
Green Fluorescent

ABSTRACTOptical coherence tomography (OCT) has gained wide adoption in biological and medical imaging due to its exceptional tissue penetration, 3D imaging speed and rich contrast. However, OCT plays a relatively small role in molecular and cellular imaging due to the lack of suitable biomolecular contrast agents. In particular, while the green fluorescent protein has provided revolutionary capabilities to fluorescence microscopy by connecting it to cellular functions such as gene expression, no equivalent reporter gene is currently available for OCT. Here we introduce gas vesicles, a unique class of naturally evolved gas-filled protein nanostructures, as the first genetically encodable OCT contrast agents. The differential refractive index of their gas compartments relative to surrounding aqueous tissue and their nanoscale motion enables gas vesicles to be detected by static and dynamic OCT at picomolar concentrations. Furthermore, the OCT contrast of gas vesicles can be selectively erasedin situwith ultrasound, allowing unambiguous assignment of their location. In addition, gas vesicle clustering modulates their temporal signal, enabling the design of dynamic biosensors. We demonstrate the use of gas vesicles as reporter genes in bacterial colonies and as purified contrast agentsin vivoin the mouse retina. Our results expand the utility of OCT as a unique photonic modality to image a wider variety of cellular and molecular processes.

scholarly journals In Vivo Imaging of Mammalian Embryos By NIR-I Photoacoustic Tomography and NIR-II Optical Coherence Tomography Using Gold Nanostars as Multifunctional Contrast Agents

2021 ◽  
Author(s):  
Sheng Zhang ◽  
Zhenyang Liu ◽  
Linlin Mao ◽  
Jian Wu ◽  
Di Zhang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Embryonic Development ◽  
Contrast Agents ◽  
Structural Characteristic ◽  
Photoacoustic Tomography ◽  
Optical Coherence ◽  
Foetal Development ◽  
Gold Nanostars ◽  
Mammalian Embryos

Abstract Background High resolution, strong contrast and multimodality visualization of live mammalian embryo is an important requirement for studying foetal development. Photoacoustic Tomography (PAT) and Optical Coherence Tomography (OCT) are two advanced imaging modalities that has been utilized for embryonic imaging. However, high contrast, multiscale and deep tissue visualization of live embryos remains challenging. Results Here, we demonstrate the use of gold nanostars (GNS) as multimodality contrast agents for the visualization and differentiation of embryos in vivo using NIR-I PAT and NIR-II OCT. We perform NIR-I PAT imaging to confirm in vivo GNS accumulation in the foetuses, and then use a customized NIR-II OCT system to further reveal deep, contrast-enhanced micro features of freshly harvested embryos. We investigate two different GNS administration pathways, i.e. intravenous and intravaginal injection, and significant enhancement of signal, image contrast, and imaging depth are achieved for both PAT and OCT. Conclusions These findings prove that PAT-OCT bi-modal imaging with GNS enhancement provides more accurate structural characteristic of live mammalian embryos, and thus reveal its potential for embryonic development visualization and early abnormality examination. These findings prove that PAT-OCT bi-modal imaging with GNS enhancement provides more accurate structural characteristic of live mammalian embryos, and thus reveal its potential for embryonic development visualization and early abnormality examination.

scholarly journals Gold Nanobipyramids as Second Near Infrared Optical Coherence Tomography Contrast Agents for Multiplexed In Vivo Lymphangiography

2019 ◽  
Author(s):  
Peng Si ◽  
Saba Shevidi ◽  
Edwin Yuan ◽  
Ke Yuan ◽  
Ziv Lautman ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Lymph Node ◽  
Contrast Agents ◽  
Near Infrared ◽  
Lymphatic Drainage ◽  
Optical Coherence ◽  
Melanoma Tumor ◽  
Contrast Enhanced ◽  
Gold Nanobipyramids

AbstractDeveloping contrast-enhanced optical coherence tomography (OCT) techniques is important for specific imaging of tissue lesions, molecular imaging, cell-tracking, and highly sensitive microangiography and lymphangiography. Multiplexed OCT imaging in the second near infrared (NIR-II) window is highly desirable since it allows simultaneous imaging and tracking of multiple biological events in high resolution with deeper tissue penetration in vivo. Here we demonstrate that gold nanobipyramids can function as OCT multiplexing contrast agents, allowing the visualization of two separate lymphatic flows occurring simultaneously from different drainage basins into the same lymph node in a live mouse. Contrast-enhanced multiplexed lymphangiography of a melanoma tumor in vivo shows that the peritumoral lymphatic drainage upstream of the tumor is unidirectional, with some drainage directly into the tumor, but the lymphatic drainage from the tumor is multi-directional. We also demonstrate real-time tracking of the contrast agents draining from a melanoma tumor specifically to the sentinel lymph node of the tumor and the three-dimensional distribution of the contrast agents in the lymph node.

Sign in / Sign up

Export Citation Format

Share Document