Real time 3D two-photon microscopy for neurology

2006 ◽  
Author(s):  
B. Rozsa ◽  
G. Katona ◽  
E. S. Vizi ◽  
A. Lukács ◽  
Z. Várallyay ◽  
...  
Keyword(s):  
Real Time ◽  
Two Photon Microscopy ◽  
Two Photon

Intravital real time imaging of liver damage and regeneration by functional two-photon microscopy

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
A Seddek ◽  
JG Hengstler
Keyword(s):  
Real Time ◽  
Liver Damage ◽  
Real Time Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

Real-time measurement of free Ca2+ changes in CNS myelin by two-photon microscopy

2007 ◽  
Vol 13 (7) ◽  
pp. 874-879 ◽  
Author(s):  
Ileana Micu ◽  
Andrew Ridsdale ◽  
Lingqing Zhang ◽  
John Woulfe ◽  
Jeff McClintock ◽  
...  
Keyword(s):  
Real Time ◽  
Time Measurement ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Real Time Measurement

scholarly journals Real-Time Two-Photon Microscopy and Its Application for In Situ Imaging

2001 ◽  
Vol 34 (6) ◽  
pp. 399-403 ◽  
Author(s):  
Tomoyuki Kaneko ◽  
Katsumasa Fujita ◽  
Hideo Tanaka ◽  
Masahito Oyamada ◽  
Osamu Nakamura ◽  
...  
Keyword(s):  
Real Time ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
In Situ Imaging

scholarly journals Intravital imaging of adriamycin-induced renal pathology using two-photon microscopy and optical coherence tomography

2018 ◽  
Vol 11 (05) ◽  
pp. 1850030 ◽  
Author(s):  
Hengchang Guo ◽  
Hsing-Wen Wang ◽  
Qinggong Tang ◽  
Erik Anderson ◽  
Reuben Falola ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
Flow Dynamics ◽  
Renal Pathology ◽  
Glomerular Sclerosis ◽  
Deep Penetration ◽  
Optical Coherence ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Tubular Flow

Adriamycin (doxorubicin), a common cancer chemotherapeutic drug, can be used to induce a model of chronic progressive glomerular disease in rodents. In our studies, we evaluated renal changes in a rat model after Adriamycin injection using two-photon microscopy (TPM), optical coherence tomography (OCT) and Doppler OCT (DOCT). Taking advantage of deep penetration and fast scanning speed for three-dimensional (3D) label-free imaging, OCT/DOCT system was able to reveal glomerular and tubular pathology noninvasively and in real time. By imaging renal pathology following the infusion of fluorophore-labeled dextrans of different molecular weights, TPM can provide direct views of glomerular and tubular flow dynamics with the onset and progression of renal disease. Specifically, glomerular permeability and filtration, proximal and distal tubular flow dynamics can be revealed. 6–8 weeks after injection of Adriamycin, TPM and OCT/DOCT imaging revealed glomerular sclerosis, compromised flow across the glomerular wall, tubular atrophy, tubular dilation, and variable intra-tubular flow dynamics. Our results indicate that TPM and OCT/DOCT provide real-time imaging of renal pathology in vivo that has not been previously available using conventional microscopic procedures.

scholarly journals Suite2p: beyond 10,000 neurons with standard two-photon microscopy

2016 ◽  
Author(s):  
Marius Pachitariu ◽  
Carsen Stringer ◽  
Mario Dipoppa ◽  
Sylvia Schröder ◽  
L. Federico Rossi ◽  
...  
Keyword(s):  
Fine Structure ◽  
Real Time ◽  
Computational Methods ◽  
State Of The Art ◽  
Two Photon Microscopy ◽  
Calcium Dependent ◽  
Two Photon ◽  
Large Populations ◽  
Previous State ◽  
Synaptic Boutons

AbstractTwo-photon microscopy of calcium-dependent sensors has enabled unprecedented recordings from vast populations of neurons. While the sensors and microscopes have matured over several generations of development, computational methods to process the resulting movies remain inefficient and can give results that are hard to interpret. Here we introduce Suite2p: a fast, accurate and complete pipeline that registers raw movies, detects active cells, extracts their calcium traces and infers their spike times. Suite2p runs on standard workstations, operates faster than real time, and recovers ~2 times more cells than the previous state-of-the-art method. Its low computational load allows routine detection of ~10,000 cells simultaneously with standard two-photon resonant-scanning microscopes. Recordings at this scale promise to reveal the fine structure of activity in large populations of neurons or large populations of subcellular structures such as synaptic boutons.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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