Two-photon microscopy for real-time monitoring of focused ultrasound-mediated drug delivery to the brain in a mouse model of Alzheimer's disease

2013 ◽  
Author(s):  
Alison Burgess ◽  
Naomi Eterman ◽  
Isabelle Aubert ◽  
Kullervo Hynynen
Keyword(s):  
Alzheimer’S Disease ◽  
Drug Delivery ◽  
Mouse Model ◽  
Real Time ◽  
Focused Ultrasound ◽  
Real Time Monitoring ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Model Of Alzheimer’S Disease ◽  
The Brain

scholarly journals Muramyl dipeptide mediated immunomodulation on monocyte subsets exerts therapeutic effects in a mouse model of Alzheimer’s disease

2020 ◽  
Author(s):  
Adham Fani Malekia ◽  
Giulia Cisbania ◽  
Marie-Michèle Plante ◽  
Paul Préfontaine ◽  
Nataly Laflamme ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Muramyl Dipeptide ◽  
Therapeutic Effects ◽  
Monocyte Subsets ◽  
Expression Levels ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Model Of Alzheimer’S Disease

Abstract Background Muramyl dipeptide (MDP) is a component derived from minimal peptidoglycan motif from bacteria and it is a ligand for the NOD2 receptor. Peripheral administration of MDP converts Ly6Chigh into Ly6Clow monocytes. Previously we have shown that Ly6Clow monocytes play crucial roles in the pathology of a mouse model of Alzheimer’s disease (AD). However, medications with mild immunomodulatory effects that solely target specific monocyte subsets, without triggering microglial activation are rare. Methods 3-months old APPswe/PS1 transgenic male mice and age-matched C57BL/6J mice were used for high frequency (2-times/week) over 6-months and low frequency (once a week) over a 3-months period of intraperitoneally MDP (10 mg/kg) administrations. Flow cytometry analysis of monocyte subsets in blood, behavioral and post mortem analyses were performed. Two-photon microscopy using APP/PS1/CX3CR1gfp/+ mice were conducted to study vascular Aβ clearance by Ly6Clow monocytes upon MDP administration.Results The treatment improved cognitive declines, increased expression levels of postsynaptic density protein 95 (PSD95) andlow density lipoprotein receptor-related protein 1 (LRP1), which are involved in synaptic plasticity and amyloid beta (Aβ) elimination, respectively. In addition, we found monocyte chemoattractant protein-1(MCP-1) levels significantly increased, whereas intercellular adhesion molecule-1(ICAM-1) significantly decreased and microglial marker (Iba1) did not change in treatment group compared to the control. In parallel, we discovered elevated cyclooxygenase-2 (COX2) expression levels in the treated group, which might be a positive factor for synaptic activity. Following MDP treatment, intravital two-photon microscopy demonstrated that Ly6Clow monocytes are recruited into the brain vasculature in APP but not wild type mice, and they are able to pick up Aβ peptides. Conclusions Our results demonstrate that MDP is beneficial in both the early phase and to some extent later phases of the pathology in the mouse model of AD. These data open the way for potential MDP-based medications for AD.

scholarly journals Whole brain vascular imaging in a mouse model of Alzheimer’s disease with two-photon microscopy

2018 ◽  
Vol 23 (07) ◽  
pp. 1 ◽  
Author(s):  
Patrick Delafontaine-Martel ◽  
Joel Lefebvre ◽  
Pier-Luc Tardif ◽  
Bernard I. Lévy ◽  
Philippe Pouliot ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Vascular Imaging ◽  
Whole Brain ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Model Of Alzheimer’S Disease

scholarly journals Antibodies Targeted to the Brain with Image-Guided Focused Ultrasound Reduces Amyloid-β Plaque Load in the TgCRND8 Mouse Model of Alzheimer's Disease

PLoS ONE ◽  
2010 ◽  
Vol 5 (5) ◽  
pp. e10549 ◽  
Author(s):  
Jessica F. Jordão ◽  
Carlos A. Ayala-Grosso ◽  
Kelly Markham ◽  
Yuexi Huang ◽  
Rajiv Chopra ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Focused Ultrasound ◽  
Amyloid Β ◽  
Image Guided ◽  
Model Of Alzheimer’S Disease ◽  
Tgcrnd8 Mouse ◽  
Plaque Load ◽  
The Brain

scholarly journals NIR-Responsive Lysosomotropic Phototrigger: ʽAIE + ESIPTʼ Active Naphthalene Based Single Component Photoresponsive Nanocarrier with Two-Photon Uncaging and Real-Time Monitoring Ability

2021 ◽  
Author(s):  
Biswajit Roy ◽  
Rakesh Mengji ◽  
Samrat Roy ◽  
Bipul Pal ◽  
Avijit Jana ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Single Component ◽  
Photon Absorption ◽  
Real Time Monitoring ◽  
Two Photon ◽  
Nir Light

In recent times, organelle-targeted drug delivery systems gained tremendous attention due to the site specific delivery of active drug molecules resulting in enhanced bioefficacy. In this context, the phototriggered drug delivery system (DDS) for releasing an active molecule is superior as it provides spatial and temporal control over the release. So far, near infrared (NIR) light responsive organelle targeted DDS has not yet been developed. Hence, we introduced a two-photon NIR-light responsive lysosome targeted ʽAIE + ESIPTʼ active single component DDS based on naphthalene chromophore. The Two-photon absorption cross-section of our DDS is 142 GM at 850 nm. The DDS was converted into pure organic nanoparticles for biological applications. Our nano-DDS is capable of selective targeting, AIE-luminogenic imaging, and drug release within the lysosome. In vitro studies using cancerous cell lines showed that our single component photoresponsive nanocarrier exhibited enhanced cytotoxicity and real-time monitoring ability of the drug release.

scholarly journals Pomegranate from Oman Alleviates the Brain Oxidative Damage in Transgenic Mouse Model of Alzheimer’s Disease

2014 ◽  
Vol 4 (4) ◽  
pp. 232-238 ◽  
Author(s):  
Selvaraju Subash ◽  
Musthafa Mohamed Essa ◽  
Abdullah Al-Asmi ◽  
Samir Al-Adawi ◽  
Ragini Vaishnav ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Oxidative Damage ◽  
Transgenic Mouse ◽  
Transgenic Mouse Model ◽  
Model Of Alzheimer’S Disease ◽  
The Brain
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