Penetration depth of fluorescence-enhanced, frequency-domain photon migration imaging in tissue phantoms

2002 ◽  
Author(s):  
Jessica P. Houston ◽  
Eva M. Sevick-Muraca
Keyword(s):  
Frequency Domain ◽  
Penetration Depth ◽  
Photon Migration ◽  
Migration Imaging ◽  
Tissue Phantoms

scholarly journals Influence of the refractive index-mismatch at the boundaries measured in fluorescenceenhanced frequency-domain photon migration imaging

2002 ◽  
Vol 10 (15) ◽  
pp. 653 ◽  
Author(s):  
Anuradha Godavarty ◽  
Daniel Hawrysz ◽  
Ranadhir Roy ◽  
Eva Sevick-Muraca ◽  
Margaret Eppstein
Keyword(s):  
Refractive Index ◽  
Frequency Domain ◽  
Photon Migration ◽  
Migration Imaging

Multipixel Techniques for Frequency-Domain Photon Migration Imaging

1997 ◽  
Vol 13 (5) ◽  
pp. 669-680 ◽  
Author(s):  
J.S. Reynolds ◽  
T.L. Troy ◽  
E.M. Sevick-Muraca
Keyword(s):  
Frequency Domain ◽  
Photon Migration ◽  
Migration Imaging

scholarly journals Sampling tissue volumes using frequency-domain photon migration

2004 ◽  
Vol 69 (5) ◽  
Author(s):  
Frédéric Bevilacqua ◽  
Joon S. You ◽  
Carole K. Hayakawa ◽  
Vasan Venugopalan
Keyword(s):  
Frequency Domain ◽  
Photon Migration

scholarly journals Absorption by water increases fluorescence image contrast of biological tissue in the shortwave infrared

2018 ◽  
Vol 115 (37) ◽  
pp. 9080-9085 ◽  
Author(s):  
Jessica A. Carr ◽  
Marianne Aellen ◽  
Daniel Franke ◽  
Peter T. C. So ◽  
Oliver T. Bruns ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Fluorescence Imaging ◽  
Biological Tissue ◽  
Ex Vivo ◽  
Scattered Light ◽  
Image Contrast ◽  
Fluorescence Image ◽  
Tissue Phantoms ◽  
Shortwave Infrared

Recent technology developments have expanded the wavelength window for biological fluorescence imaging into the shortwave infrared. We show here a mechanistic understanding of how drastic changes in fluorescence imaging contrast can arise from slight changes of imaging wavelength in the shortwave infrared. We demonstrate, in 3D tissue phantoms and in vivo in mice, that light absorption by water within biological tissue increases image contrast due to attenuation of background and highly scattered light. Wavelengths of strong tissue absorption have conventionally been avoided in fluorescence imaging to maximize photon penetration depth and photon collection, yet we demonstrate that imaging at the peak absorbance of water (near 1,450 nm) results in the highest image contrast in the shortwave infrared. Furthermore, we show, through microscopy of highly labeled ex vivo biological tissue, that the contrast improvement from water absorption enables resolution of deeper structures, resulting in a higher imaging penetration depth. We then illustrate these findings in a theoretical model. Our results suggest that the wavelength-dependent absorptivity of water is the dominant optical property contributing to image contrast, and is therefore crucial for determining the optimal imaging window in the infrared.

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