scholarly journals Experimental and theoretical study of light scattering by individual mature red blood cells by use of scanning flow cytometry and a discrete dipole approximation

2005 ◽  
Vol 44 (25) ◽  
pp. 5249 ◽  
Author(s):  
Maxim A. Yurkin ◽  
Konstantin A. Semyanov ◽  
Peter A. Tarasov ◽  
Andrei V. Chernyshev ◽  
Alfons G. Hoekstra ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Light Scattering ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Theoretical Study ◽  
Dipole Approximation ◽  
Discrete Dipole Approximation

Large Scale Simulations of Elastic Light Scattering by a Fast Discrete Dipole Approximation

1998 ◽  
Vol 09 (01) ◽  
pp. 87-102 ◽  
Author(s):  
A. G. Hoekstra ◽  
M. D. Grimminck ◽  
P. M. A. Sloot
Keyword(s):  
Light Scattering ◽  
Blood Cells ◽  
Large Scale ◽  
Incident Light ◽  
Spherical Inclusion ◽  
White Blood Cells ◽  
Dipole Approximation ◽  
Discrete Dipole Approximation ◽  
Dust Particles ◽  
Elastic Light Scattering

Simulation of Elastic Light Scattering from arbitrary shaped particles in the resonance region (i.e., with a dimension of several wavelengths of the incident light) is a long standing challenge. By employing the combination of a simulation kernel with low computational complexity, implemented on powerful High Performance Computing systems, we are now able to push the limits of simulation of scattering of visible light towards particles with dimensions up to 10 micrometers. This allows for the first time the simulation of realistic and highly relevant light scattering experiments, such as scattering from human red — or white blood cells, or scattering from large soot — or dust particles. We use the Discrete Dipole Approximation to simulate the light scattering process. In this paper we report on a parallel Fast Discrete Dipole Approximation, and we will show the performance of the resulting code, running under PVM on a 32-node Parsytec CC. Furthermore, as an example we present results of a simulation of scattering from human white blood cells. In a first approximation the Lymphocyte is modeled as a sphere with a spherical inclusion. We investigate the influence of the position of the inner sphere, modeling the nucleus of a Lymphocyte, on the light scattering signals.

Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools

Vox Sanguinis ◽  
2008 ◽  
Vol 95 (4) ◽  
pp. 288-297 ◽  
Author(s):  
O. Rubin ◽  
D. Crettaz ◽  
G. Canellini ◽  
J.-D. Tissot ◽  
N. Lion
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells

scholarly journals Necrotic and apoptotic volume changes of red blood cells investigated by low-angle light scattering technique

2007 ◽  
Vol 21 (2) ◽  
pp. 105-120 ◽  
Author(s):  
Igor V. Mindukshev ◽  
Vladimir V. Krivoshlyk ◽  
Elena E. Ermolaeva ◽  
Irina A. Dobrylko ◽  
Evgeniy V. Senchenkov ◽  
...  
Keyword(s):  
Light Scattering ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Platelets ◽  
Calcium Ionophore ◽  
Scattering Technique ◽  
Rbc Aggregation ◽  
Ammonia Medium ◽  
Very High ◽  
Light Scattering Technique

A low-angle light scattering technique, which has been applied previously to studies of blood platelets and Ehrlich ascite tumor cells, revealed differences in the dynamics of necrotic and apoptotic red blood cell death. Under hypotonic loading or in ammonia medium, red blood cells (RBC) swelled to a critical size (diameter approximately 13μm) prior to hemolysis (necrosis). Under acidic loading, hemolysis occurred with less pronounced swelling of cells (diameter approximately 10μm). Apoptosis induced by a calcium ionophore resulted in initial formation of echinocytes, followed by development of rounded red blood cells with uneven membrane, capable of agglomeration. In such a way, RBC aggregation can precede the final stages of the RBC apoptosis when small cellular fragments are generated. On the basis of erythrograms of the cells hemolysing in ammonia medium, the echinocytic (preapoptotic) and stomatocytic (prenecrotic) RBC were discerned due to the very high resistance of apoptotic RBC to osmotic (ammonia) loading.

Simultaneous determination of size and refractive index of red blood cells by light scattering measurements

2006 ◽  
Vol 88 (8) ◽  
pp. 084101 ◽  
Author(s):  
N. Ghosh ◽  
P. Buddhiwant ◽  
A. Uppal ◽  
S. K. Majumder ◽  
H. S. Patel ◽  
...  
Keyword(s):  
Refractive Index ◽  
Light Scattering ◽  
Red Blood Cells ◽  
Simultaneous Determination ◽  
Blood Cells ◽  
Scattering Measurements

Improved quantitative detection of biotin‐labeled red blood cells by flow cytometry

Transfusion ◽  
2019 ◽  
Vol 59 (8) ◽  
pp. 2691-2698 ◽  
Author(s):  
Albert D. Donnenberg ◽  
Tamir Kanias ◽  
Darrell J. Triulzi ◽  
Catherine J. Dennis ◽  
E. Michael Meyer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Quantitative Detection
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