Diagnostics of functional state of blood by registration of light scattering intensity variations due to the reversible aggregation of red blood cells

1991 ◽  
Author(s):  
R. T. Tukhvatulin ◽  
P. P. Vaulin
Keyword(s):  
Light Scattering ◽  
Red Blood Cells ◽  
Functional State ◽  
Blood Cells ◽  
Scattering Intensity ◽  
Reversible Aggregation ◽  
Light Scattering Intensity

Light Scattering Intensity Fluctuations in Microdroplets Containing Inclusions.

1997 ◽  
Author(s):  
Gorden Videen ◽  
Paul Pellegrino ◽  
Dat Ngo ◽  
John S. Videen ◽  
Ronald G. Pinnick
Keyword(s):  
Light Scattering ◽  
Scattering Intensity ◽  
Light Scattering Intensity ◽  
Intensity Fluctuations

scholarly journals Necrotic and apoptotic volume changes of red blood cells investigated by low-angle light scattering technique

2007 ◽  
Vol 21 (2) ◽  
pp. 105-120 ◽  
Author(s):  
Igor V. Mindukshev ◽  
Vladimir V. Krivoshlyk ◽  
Elena E. Ermolaeva ◽  
Irina A. Dobrylko ◽  
Evgeniy V. Senchenkov ◽  
...  
Keyword(s):  
Light Scattering ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Platelets ◽  
Calcium Ionophore ◽  
Scattering Technique ◽  
Rbc Aggregation ◽  
Ammonia Medium ◽  
Very High ◽  
Light Scattering Technique

A low-angle light scattering technique, which has been applied previously to studies of blood platelets and Ehrlich ascite tumor cells, revealed differences in the dynamics of necrotic and apoptotic red blood cell death. Under hypotonic loading or in ammonia medium, red blood cells (RBC) swelled to a critical size (diameter approximately 13μm) prior to hemolysis (necrosis). Under acidic loading, hemolysis occurred with less pronounced swelling of cells (diameter approximately 10μm). Apoptosis induced by a calcium ionophore resulted in initial formation of echinocytes, followed by development of rounded red blood cells with uneven membrane, capable of agglomeration. In such a way, RBC aggregation can precede the final stages of the RBC apoptosis when small cellular fragments are generated. On the basis of erythrograms of the cells hemolysing in ammonia medium, the echinocytic (preapoptotic) and stomatocytic (prenecrotic) RBC were discerned due to the very high resistance of apoptotic RBC to osmotic (ammonia) loading.

Simultaneous determination of size and refractive index of red blood cells by light scattering measurements

2006 ◽  
Vol 88 (8) ◽  
pp. 084101 ◽  
Author(s):  
N. Ghosh ◽  
P. Buddhiwant ◽  
A. Uppal ◽  
S. K. Majumder ◽  
H. S. Patel ◽  
...  
Keyword(s):  
Refractive Index ◽  
Light Scattering ◽  
Red Blood Cells ◽  
Simultaneous Determination ◽  
Blood Cells ◽  
Scattering Measurements

Assessing the Functional State of Red Blood Cells by Using the Laser Interference Microscopy

2021 ◽  
Vol 15 (4) ◽  
pp. 388-392
Author(s):  
A. V. Deryugina ◽  
M. N. Ivashchenko ◽  
A. A. Belov ◽  
P. S. Ignatiev ◽  
V. B. Metelin
Keyword(s):  
Red Blood Cells ◽  
Functional State ◽  
Blood Cells ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

Some Effects on Light Scattering Intensity and Red Blood Cell Size Distribution Histograms Due to Sphering

1988 ◽  
Vol 25 (6) ◽  
pp. 673-679 ◽  
Author(s):  
P C Bartels ◽  
P W Helleman ◽  
A F M Roijers ◽  
J B J Soons
Keyword(s):  
Light Scattering ◽  
Blood Cell ◽  
Size Distribution ◽  
Red Blood Cell ◽  
Signal To Noise Ratio ◽  
Quantitative Information ◽  
Cell Size Distribution ◽  
Scattering Intensity ◽  
Distribution Ranges ◽  
Light Scattering Intensity

At present most haematology blood cell analysers routinely provide red blood cell (RBC) size distribution histograms. Sophisticated improvements of the instruments have re-awakened interest in the study of size histograms. The quantitative information derived from the histograms may be applied more fruitfully if insight is available, with respect to some essential principles of sizing technology and methods for treatment of RBCs before measurement. In this study the consequences of sphering RBCs are investigated in relation to the generation of size distribution histograms by means of methods based on light scattering intensity (LSI). Sphering of RBCs results in considerably narrower histograms than unsphered RBCs. The overall signal to noise ratio increases and there is a broader gap between large platelets and microcytic RBCs. Narrower size distribution ranges will enable closer modes to be separated. Compared to unsphered RBCs, microcytic sphered RBCs yield increased LSI whereas macrocytic sphered RBCs yield decreased LSI.

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