scholarly journals Bronchioloalveolar Carcinoma Not Related to Jaagsiekte Sheep Retrovirus in a Goat

2007 ◽  
Vol 44 (5) ◽  
pp. 710-712 ◽  
Author(s):  
A. Ortin ◽  
A. A. Benito ◽  
D. Lacasta ◽  
L. M. Ferrer ◽  
M. De Las Heras
Keyword(s):  
Lung Tumor ◽  
Clinical Signs ◽  
Bronchioloalveolar Carcinoma ◽  
Alveolar Type ◽  
Chain Reaction ◽  
Jaagsiekte Sheep Retrovirus ◽  
Enzootic Nasal Tumor Virus ◽  
Tumor Virus ◽  
Nasal Tumor ◽  
Polymerase Chain

A spontaneous lung tumor in a 5–year-old goat of the Murciano-Granadina breed is described in this paper. Clinical signs of cachexia and tachypnoea were evident, and a considerable amount of white mucous foamy fluid was discharged from the nostrils when the animal's head was lowered. A lung tumor with the characteristics of bronchioloalveolar carcinoma was detected during histopathologic examination. The tumor cells were positive for surfactant proteins C and B, confirming that alveolar type II cells were the origin of the neoplasia. Tumor samples were tested by polymerase chain reaction, immunoblotting, and immunohistochemistry for the presence of Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV), another retrovirus very closely related to JSRV, but all tests were negative. Therefore, this is the first reported case of spontaneous bronchioloalveolar carcinoma not related to JSRV or ENTV infection in a goat.

scholarly journals The U3 and Env Proteins of Jaagsiekte Sheep Retrovirus and Enzootic Nasal Tumor Virus Both Contribute to Tissue Tropism

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1061 ◽  
Author(s):  
Rosales Gerpe ◽  
van Lieshout ◽  
Domm ◽  
Ingrao ◽  
Datu ◽  
...  
Keyword(s):  
Amino Acid Identity ◽  
Tissue Tropism ◽  
Genetic Determinants ◽  
Tissue Slices ◽  
Jaagsiekte Sheep Retrovirus ◽  
Nasal Turbinate ◽  
Show Evidence ◽  
Enzootic Nasal Tumor Virus ◽  
Tumor Virus ◽  
Nasal Tumor

Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV) are small-ruminant betaretroviruses that share high nucleotide and amino acid identity, utilize the same cellular receptor, hyaluronoglucosaminidase 2 (Hyal2) for entry, and transform tissues with their envelope (Env) glycoprotein; yet, they target discrete regions of the respiratory tract—the lung and nose, respectively. This distinct tissue selectivity makes them ideal tools with which to study the pathogenesis of betaretroviruses. To uncover the genetic determinants of tropism, we constructed JSRV–ENTV chimeric viruses and produced lentivectors pseudotyped with the Env proteins from JSRV (Jenv) and ENTV (Eenv). Through the transduction and infection of lung and nasal turbinate tissue slices, we observed that Hyal2 expression levels strongly influence ENTV entry, but that the long terminal repeat (LTR) promoters of these viruses are likely responsible for tissue-specificity. Furthermore, we show evidence of ENTV Env expression in chondrocytes within ENTV-infected nasal turbinate tissue, where Hyal2 is highly expressed. Our work suggests that the unique tissue tropism of JSRV and ENTV stems from the combined effort of the envelope glycoprotein-receptor interactions and the LTR and provides new insight into the pathogenesis of ENTV.

scholarly journals First case of nasal transitional carcinoma in a goat infected with Enzootic Nasal Tumor Virus in Belgium

2020 ◽  
Vol 89 (4) ◽  
pp. 226-230
Author(s):  
A. Delaude ◽  
E. Raes ◽  
C. Leroux ◽  
K. Chiers ◽  
L. Sonck
Keyword(s):  
Nasal Passage ◽  
Neoplastic Tissue ◽  
Computed Tomographic ◽  
First Case ◽  
Enzootic Nasal Tumor Virus ◽  
Tumor Virus ◽  
Nasal Tumor ◽  
Retrobulbar Space ◽  
Polymerase Chain ◽  
The Right

An eleven-year-old, female goat was presented for evaluation of breathing difficulties and epistaxis. Radiographs and computed tomographic (CT) examination of the head revealed the presence of a space-occupying lesion involving the right nasal passage and invading the cranial vault and retrobulbar space. Histologic examination and detection of viral genome from the nasal mass led to the diagnosis of nasal transitional carcinoma with concomitant infection with Enzootic Nasal Tumor Virus (ENTV-2). In this case report, the presence of a nasal transitional carcinoma is described in a goat; a very rare tumor which, to the authors’ knowledge, has not been previously reported in goats. Reverse transcription polymerase chain reaction (RT-PCR) detected the genome of ENTV-2 within the neoplastic tissue, suggesting an infectious etiology.

scholarly journals Envelope Proteins of Jaagsiekte Sheep Retrovirus and Enzootic Nasal Tumor Virus Induce Similar Bronchioalveolar Tumors in Lungs of Mice

2006 ◽  
Vol 80 (18) ◽  
pp. 9322-9325 ◽  
Author(s):  
Sarah K. Wootton ◽  
Christine L. Halbert ◽  
A. Dusty Miller
Keyword(s):  
Airway Epithelial Cells ◽  
Tumor Formation ◽  
Airway Epithelial ◽  
Jaagsiekte Sheep Retrovirus ◽  
Sheep And Goats ◽  
Specific Tumor ◽  
Enzootic Nasal Tumor Virus ◽  
Tumor Virus ◽  
Nasal Tumor ◽  
Env Protein

ABSTRACT Jaagsiekte sheep retrovirus (JSRV) induces bronchioalveolar tumors in sheep and goats. Expression of the JSRV envelope (Env) protein in mouse airway epithelial cells induces similar tumors, indicating that Env expression is sufficient for tissue-specific tumor formation. Enzootic nasal tumor virus (ENTV) is related to JSRV but induces tumors in the nasal epithelium of sheep and goats. Here we found that ENTV Env can also induce tumors in mice but, unexpectedly, with a phenotype identical to that of tumors induced by the JSRV Env, indicating that factors other than Env mediate the tissue specificity of tumor induction by ENTV.

scholarly journals First case of nasal transitional carcinoma in a goat infected with Enzootic Nasal Tumor Virus in Belgium

2020 ◽  
Vol 89 (4) ◽  
pp. 226-230
Author(s):  
A. Delaude ◽  
E. Raes ◽  
C. Leroux ◽  
K. Chiers ◽  
L. Sonck
Keyword(s):  
Nasal Passage ◽  
Neoplastic Tissue ◽  
Computed Tomographic ◽  
First Case ◽  
Enzootic Nasal Tumor Virus ◽  
Tumor Virus ◽  
Nasal Tumor ◽  
Retrobulbar Space ◽  
Polymerase Chain ◽  
The Right

An eleven-year-old, female goat was presented for evaluation of breathing difficulties and epistaxis. Radiographs and computed tomographic (CT) examination of the head revealed the presence of a space-occupying lesion involving the right nasal passage and invading the cranial vault and retrobulbar space. Histologic examination and detection of viral genome from the nasal mass led to the diagnosis of nasal transitional carcinoma with concomitant infection with Enzootic Nasal Tumor Virus (ENTV-2). In this case report, the presence of a nasal transitional carcinoma is described in a goat; a very rare tumor which, to the authors’ knowledge, has not been previously reported in goats. Reverse transcription polymerase chain reaction (RT-PCR) detected the genome of ENTV-2 within the neoplastic tissue, suggesting an infectious etiology.

scholarly journals Complete Sequence of Enzootic Nasal Tumor Virus, a Retrovirus Associated with Transmissible Intranasal Tumors of Sheep

1999 ◽  
Vol 73 (5) ◽  
pp. 3986-3993 ◽  
Author(s):  
Christina Cousens ◽  
Esmeralda Minguijon ◽  
Robert G. Dalziel ◽  
Aurora Ortin ◽  
Mercedes Garcia ◽  
...  
Keyword(s):  
Complete Genome ◽  
Complete Sequence ◽  
Endogenous Retroviruses ◽  
Type B ◽  
Jaagsiekte Sheep Retrovirus ◽  
Genetic Organization ◽  
Enzootic Nasal Tumor Virus ◽  
Tumor Virus ◽  
Nasal Tumor

ABSTRACT The sequence of the complete genome of ovine enzootic nasal tumor virus, an exogenous retrovirus associated exclusively with contagious intranasal tumors of sheep, was determined. The genome is 7,434 nucleotides long and exhibits a genetic organization characteristic of type B and D oncoviruses. Enzootic nasal tumor virus is closely related to the Jaagsiekte sheep retrovirus and to sheep endogenous retroviruses.

scholarly journals Nasal adenocarcinoma associated with jaagsiekte sheep retrovirus infection in a sheep

2019 ◽  
Vol 32 (1) ◽  
pp. 152-155 ◽  
Author(s):  
Hanne Jahns ◽  
Chris Cousens
Keyword(s):  
Animal Health ◽  
Health Impact ◽  
Jaagsiekte Sheep Retrovirus ◽  
Enzootic Nasal Tumor Virus ◽  
Microscopic Features ◽  
Tumor Virus ◽  
Nasal Tumor ◽  
Nasal Adenocarcinoma ◽  
Anatomic Locations ◽  
Retrovirus Infection

Betaretrovirus-induced transmissible respiratory tumors in sheep arise at 2 distinct anatomic locations, either deep in the lung tissue caused by jaagsiekte sheep retrovirus (JSRV) or in the nasal cavity induced by ovine enzootic nasal tumor virus (ENTV-1). JSRV and ENTV-1 are found in many countries worldwide and have a significant economic and animal health impact. Although JSRV is endemic in sheep in the British Isles, ENTV-1 has not been reported. We report herein a nasal adenocarcinoma in a cull 8-y-old Belclare ewe from Ireland. The gross and microscopic features and immunohistochemistry results were consistent with an ENTV-1–associated tumor. However, differential PCR, using primers specific to regions of divergent sequence between the viruses, was performed on different parts of the adenocarcinoma and produced consistent results: positive for JSRV and negative for ENTV-1. An association of JSRV with nasal adenocarcinoma in sheep has not been reported previously, to our knowledge. Our case shows the necessity of using PCR in combination with immunohistochemistry to reach an accurate etiologic diagnosis, which is of importance in countries currently free of ENTV-1.

scholarly journals Comparison of Three Rapid Commercial Canine Parvovirus Antigen Detection Tests with Electron Microscopy and Polymerase Chain Reaction

2009 ◽  
Vol 21 (3) ◽  
pp. 344-345 ◽  
Author(s):  
Silke Schmitz ◽  
Christina Coenen ◽  
König Matthias ◽  
Thiel Heinz-Jürgen ◽  
Reto Neiger
Keyword(s):  
Electron Microscopy ◽  
Polymerase Chain Reaction ◽  
Chronic Diarrhea ◽  
Gastrointestinal Disease ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Chain Reaction ◽  
Group A ◽  
Polymerase Chain ◽  
Group B

Different antibody-based tests for rapid detection of Canine parvovirus antigens in feces are commercially available, allowing quick diagnosis in a clinical setting. However, the diagnostic accuracy of these tests compared with standard methods has not been evaluated so far. In the current study, 3 commercial tests were compared with immune-electron microscopy (IEM) and polymerase chain reaction (PCR). Dogs were divided into 3 groups: group A, samples from dogs with acute hemorrhagic diarrhea ( n = 50); group B, dogs with chronic diarrhea ( n = 10); and group C, dogs with no evidence of gastrointestinal disease ( n = 40). Specificity of all 3 commercial tests versus PCR and IEM was good to excellent (92.2–100%). Sensitivity, in contrast, was poor: 15.8–26.3% versus PCR and 50–60% versus IEM. In group A, 10 dogs were positive by IEM and 24 dogs were positive by PCR. Positive PCR results were also obtained from animals in control groups (group B, 1 dog; group C, 5 dogs). No dog in group B or C was positive by IEM. In conclusion, the rapid tests are useful to diagnose canine parvoviral enteritis, but they do not rule out parvovirus infection in an animal with typical clinical signs. In addition, a small percentage of healthy dogs and dogs with chronic diarrhea showed positive PCR results; this may be due to asymptomatic/persistent infection or intestinal passage of virus. The significance of this finding remains unclear.

scholarly journals First report of Trypanosoma cruziinfection in naturally infected dogs from southern Bahia, Brazil

2013 ◽  
Vol 22 (1) ◽  
pp. 182-185 ◽  
Author(s):  
Nilo Fernandes Leça Júnior ◽  
Valter dos Anjos Almeida ◽  
Fábio Santos Carvalho ◽  
George Rego Albuquerque ◽  
Fabiana Lessa Silva
Keyword(s):  
Endemic Area ◽  
Natural Infection ◽  
Clinical Signs ◽  
Domestic Dogs ◽  
Chain Reaction ◽  
First Report ◽  
Molecular Tests ◽  
Southern Bahia ◽  
Polymerase Chain ◽  
Cruzi Infection

In order to verify the Trypanosoma cruzi infection in domestic domiciled dogs in a rural endemic area from the south region of the State of Bahia, Polymerase Chain Reaction (PCR) were performed using S35 and S36 primers in 272 dogs living in the district of Vila Operaria, in the municipality of Buerarema. All animals were clinically evaluated; 2.5 mL of blood were collected through venipuncture for the performance of molecular tests. None of these animals showed clinical signs of the illness and only two were identified with the DNA parasite. This result is the first report of natural infection by T. cruzi in domestic dogs in southern Bahia.

Molecular characterisation of ovine herpesvirus type 2 (OvHV-2) in Turkey

2012 ◽  
Vol 60 (4) ◽  
pp. 521-527 ◽  
Author(s):  
Yakup Yildirim ◽  
Seval Bilge Dağalp ◽  
Volkan Yilmaz ◽  
Ali Faraji Majarashin
Keyword(s):  
Clinical Signs ◽  
Peripheral Blood Leukocyte ◽  
Malignant Catarrhal Fever ◽  
Molecular Characterisation ◽  
Laboratory Examination ◽  
Chain Reaction ◽  
Herpesvirus Type ◽  
Polymerase Chain ◽  
Blood Leukocyte

In this study, the physical examination of 22 cattle revealed clinical signs of malignant catarrhal fever (MCF). Peripheral blood leukocyte (PBL) samples of the 22 cattle, and nasal (n = 7) and conjunctival (n = 9) swab samples from 16 sheep from two different farms, were taken for laboratory examination. The clinical diagnosis of MCF in cows was confirmed by the detection of ovine herpesvirus type 2 (OvHV-2) DNA by polymerase chain reaction (PCR). OvHV-2 DNA was detected by nested-PCR in PBL of one cow with clinical signs and nasal (1/7)-conjunctival(1/9) swab samples of two sheep housed in the same barn. According to the sequence analysis, three slightly divergent viruses were detected. The results indicate the need for additional research in different regions of Turkey to gain a better understanding of the incidence of MCF and its implications for the livestock industry.

Sign in / Sign up

Export Citation Format

Share Document