Molecular characterisation of ovine herpesvirus type 2 (OvHV-2) in Turkey

2012 ◽  
Vol 60 (4) ◽  
pp. 521-527 ◽  
Author(s):  
Yakup Yildirim ◽  
Seval Bilge Dağalp ◽  
Volkan Yilmaz ◽  
Ali Faraji Majarashin
Keyword(s):  
Clinical Signs ◽  
Peripheral Blood Leukocyte ◽  
Malignant Catarrhal Fever ◽  
Molecular Characterisation ◽  
Laboratory Examination ◽  
Chain Reaction ◽  
Herpesvirus Type ◽  
Polymerase Chain ◽  
Blood Leukocyte

In this study, the physical examination of 22 cattle revealed clinical signs of malignant catarrhal fever (MCF). Peripheral blood leukocyte (PBL) samples of the 22 cattle, and nasal (n = 7) and conjunctival (n = 9) swab samples from 16 sheep from two different farms, were taken for laboratory examination. The clinical diagnosis of MCF in cows was confirmed by the detection of ovine herpesvirus type 2 (OvHV-2) DNA by polymerase chain reaction (PCR). OvHV-2 DNA was detected by nested-PCR in PBL of one cow with clinical signs and nasal (1/7)-conjunctival(1/9) swab samples of two sheep housed in the same barn. According to the sequence analysis, three slightly divergent viruses were detected. The results indicate the need for additional research in different regions of Turkey to gain a better understanding of the incidence of MCF and its implications for the livestock industry.

scholarly journals Feline panleukopenia virus revisited : molecular characteristics and pathological lesions associated with three recent isolates

2000 ◽  
Vol 71 (3) ◽  
Author(s):  
M. Van Vuuren ◽  
A. Steinel ◽  
T. Goosen ◽  
E. Lane ◽  
J. Van der Lugt ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Domestic Cat ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Feline Panleukopenia Virus ◽  
Pathological Lesions ◽  
Polymerase Chain ◽  
Low Incidence ◽  
Canine Parvovirus Type 2

The low incidence of clinical signs or pathological lesions compatible with feline panleukopenia in cats has created the perception among practitioners that the disease has disappeared since the emergence of canine parvovirus type 2 in the late 1970s.Three parvoviruses that were recently isolated from a domestic cat and 2 cheetahs in cell culture or detected by means of the polymerase chain reaction were shown to be typical feline parvoviruses. Phylogenetic comparison with other FPV isolates did not reveal a particular African cluster.

scholarly journals Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR

2010 ◽  
Vol 30 (11) ◽  
pp. 918-920 ◽  
Author(s):  
Priscilla F Gerber ◽  
Flávia F Pinto ◽  
Marcos B Heinemann ◽  
Zélia I.P Lobato
Keyword(s):  
Real Time ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Real Time Quantitative Pcr ◽  
Polymerase Chain

The dynamics of porcine circovirus type 2 (PCV2) shedding in semen of naturally infected boars was studied. Semen was collected serially each 15 or 20 days during 62 days from 5 boars from a herd and from 11 boars from an artificial insemination center. All boars were positive for PCV2 DNA by nested polymerase chain reaction of raw semen in at least two sampling dates, and most of them had detectable shedding in all sampling dates. Real-time quantitative PCR was performed in 23 samples. All samples showed low amounts of PCV2 DNA, ranging from 98 to 652 PCV2 copies/mL. No differences between the frequencies of PCV2 DNA shed in semen were found considering herds and age of boars. PCV2 shedding in the semen can occur continuously or intermittently up to 60 days in naturally infected boars at 12 to 42 months old in absence of PCV2 clinical signs. These results demonstrate sporadic and long-term shedding patterns of low amounts of PCV2 DNA in semen from naturally infected boars.

Ibex-Associated Malignant Catarrhal Fever in Duikers (Cephalophus Spp)

2020 ◽  
Vol 57 (4) ◽  
pp. 577-581
Author(s):  
Francisco R. Carvallo ◽  
Francisco A. Uzal ◽  
Janet D. Moore ◽  
Kenneth Jackson ◽  
Akinyi C. Nyaoke ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Clinical Signs ◽  
Malignant Catarrhal Fever ◽  
Chain Reaction ◽  
Viral Dna ◽  
Capra Ibex ◽  
Virus Sequence ◽  
Polymerase Chain

Eight duikers, representing 3 different species cohoused in a single zoological collection, died in a 10-month period. Black, red-flanked, and yellow-backed duikers were affected, appearing clinically with a combination of anorexia, diarrhea, ataxia, tremors, and/or stupor, followed by death within 72 hours of onset of clinical signs. Consistent gross findings were pulmonary ecchymoses (8/8), generalized lymphadenomegaly (6/8), ascites (5/8), and pleural effusion (4/8). Dense lymphocyte infiltrates and arteritis affected numerous tissues in most animals. Ibex-associated malignant catarrhal fever (MCF) viral DNA was detected in all cases by polymerase chain reaction and in situ hybridization. Identical ibex-MCF virus sequence was detected in spleen of a clinically healthy ibex ( Capra ibex) housed in a separate enclosure 35 meters away from the duikers.

scholarly journals Comparison of Three Rapid Commercial Canine Parvovirus Antigen Detection Tests with Electron Microscopy and Polymerase Chain Reaction

2009 ◽  
Vol 21 (3) ◽  
pp. 344-345 ◽  
Author(s):  
Silke Schmitz ◽  
Christina Coenen ◽  
König Matthias ◽  
Thiel Heinz-Jürgen ◽  
Reto Neiger
Keyword(s):  
Electron Microscopy ◽  
Polymerase Chain Reaction ◽  
Chronic Diarrhea ◽  
Gastrointestinal Disease ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Chain Reaction ◽  
Group A ◽  
Polymerase Chain ◽  
Group B

Different antibody-based tests for rapid detection of Canine parvovirus antigens in feces are commercially available, allowing quick diagnosis in a clinical setting. However, the diagnostic accuracy of these tests compared with standard methods has not been evaluated so far. In the current study, 3 commercial tests were compared with immune-electron microscopy (IEM) and polymerase chain reaction (PCR). Dogs were divided into 3 groups: group A, samples from dogs with acute hemorrhagic diarrhea ( n = 50); group B, dogs with chronic diarrhea ( n = 10); and group C, dogs with no evidence of gastrointestinal disease ( n = 40). Specificity of all 3 commercial tests versus PCR and IEM was good to excellent (92.2–100%). Sensitivity, in contrast, was poor: 15.8–26.3% versus PCR and 50–60% versus IEM. In group A, 10 dogs were positive by IEM and 24 dogs were positive by PCR. Positive PCR results were also obtained from animals in control groups (group B, 1 dog; group C, 5 dogs). No dog in group B or C was positive by IEM. In conclusion, the rapid tests are useful to diagnose canine parvoviral enteritis, but they do not rule out parvovirus infection in an animal with typical clinical signs. In addition, a small percentage of healthy dogs and dogs with chronic diarrhea showed positive PCR results; this may be due to asymptomatic/persistent infection or intestinal passage of virus. The significance of this finding remains unclear.

scholarly journals First report of Trypanosoma cruziinfection in naturally infected dogs from southern Bahia, Brazil

2013 ◽  
Vol 22 (1) ◽  
pp. 182-185 ◽  
Author(s):  
Nilo Fernandes Leça Júnior ◽  
Valter dos Anjos Almeida ◽  
Fábio Santos Carvalho ◽  
George Rego Albuquerque ◽  
Fabiana Lessa Silva
Keyword(s):  
Endemic Area ◽  
Natural Infection ◽  
Clinical Signs ◽  
Domestic Dogs ◽  
Chain Reaction ◽  
First Report ◽  
Molecular Tests ◽  
Southern Bahia ◽  
Polymerase Chain ◽  
Cruzi Infection

In order to verify the Trypanosoma cruzi infection in domestic domiciled dogs in a rural endemic area from the south region of the State of Bahia, Polymerase Chain Reaction (PCR) were performed using S35 and S36 primers in 272 dogs living in the district of Vila Operaria, in the municipality of Buerarema. All animals were clinically evaluated; 2.5 mL of blood were collected through venipuncture for the performance of molecular tests. None of these animals showed clinical signs of the illness and only two were identified with the DNA parasite. This result is the first report of natural infection by T. cruzi in domestic dogs in southern Bahia.

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