scholarly journals Evaluation of Phylogenetic Relationships of Some Medicinally Important Species of Solanum Based on Seed Protein Profile of SDS-PAGE

2020 ◽  
Vol 8 (5) ◽  
pp. 439-446
Author(s):  
Anirban Paul ◽  
Nirmalya Banerjee
Keyword(s):  
Phylogenetic Relationships ◽  
Seed Protein ◽  
Protein Profile ◽  
Important Species ◽  
Sds Page

scholarly journals Evaluation of Genetic Diversity of Seven Species of Solanum in Nigeria Using SDS-PAGE

2019 ◽  
pp. 1-6
Author(s):  
Busirat Odunayo Ogundairo ◽  
Muyiwa Segun Ayodele
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Seed Protein ◽  
Protein Profile ◽  
Generic Status ◽  
Molecular Weights ◽  
Sds Page ◽  
The Family ◽  
Intraspecific Relationships ◽  
Poly Acrylamide

Seed protein profile of 12 accessions belonging to 7 species of the genus Solanum of the family solanaceae were investigated through poly acrylamide gel electrophoresis. The accessions were selected based on their overlapping morphological associations observed on the field. Occurrence of two protein bands of molecular weights 100 kda and 20 kda in all accession showed interspecific relationships and could be used as generic bands among the genus Solanum. All morphologically similar accessions manifested similarities in their protein bands except S. melongena. Dendrogram based on upgma also revealed the generic status and intraspecific relationships of the accession of S. aethiopicum and S. macrocarpon.

scholarly journals Revealing Antioxidant and Antidiabetic Potency of Melinjo (Gnetum Gnemon) Seed Protein Hydrolysate at Different Stages of Seed Maturation

2019 ◽  
Vol 7 (2) ◽  
pp. 479-487 ◽  
Author(s):  
Anang Supriyadi ◽  
Laras Sekar Arum ◽  
Ari Satia Nugraha ◽  
Anak Agung Istri Ratnadewi ◽  
Tri Agus Siswoyo
Keyword(s):  
Seed Protein ◽  
Protein Hydrolysate ◽  
Protein Profile ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antidiabetic Activity ◽  
Degree Of Hydrolysis ◽  
Protein Extract ◽  
Sds Page ◽  
Gnetum Gnemon

roteins hydrolyzed from melinjo seeds (Gnetum gnemon) at green (GM), yellow (YM) and red (RM) stages of maturity were studied for their effectiveness in antioxidant and antidiabetic activities. The seed protein extract was hydrolyzed using alcalase 2.4L, and the resulting hydrolysates with the highest degree of hydrolysis, protein profile, and the most potent contributors to antioxidant and invitro antidiabetic activities were identified. The degree of hydrolysis value of hydrolysates ranged from 52-84%, and the SDS-PAGE protein profile showed two distinct bands in which the band with molecular weight of 30 kDa degraded more intensively. Antioxidant capacity was measured using different standard methods, including radical cation 2,2-azinobis-(3-ethylbenzothizoline-6-sulphonate) (ABTS•+) assay, hydroxyl radical (OH•), and superoxide anion (O2•-) scavenging. The green hydrolysate (GMH) had significantly higher (p<0.05) free radical scavenging (ABTS•+, OH•, and O2•-) activities than that of the yellow hydrolysate (YMH) and red hydrolysate (RMH). However, invitro antidiabetic testing was performed based on the inhibitory activity of α-amylase and α-glucosidase. GMH was found to be more effective than YMH and RMH. These results showed that the antioxidant and antidiabetic activity in hydrolyzed GM protein has high potential to be utilized as natural nutraceuticals.

scholarly journals Influence of Rhizobacterium Inoculation on NaCl Salinity Tolerance in Pusa Sukomal and RC101 Varieties of Cowpea (Vigna unguiculata L.)

2017 ◽  
Vol 03 ◽  
pp. 35
Author(s):  
Sadhna Chaturvedi ◽  
Tejovathi Gudipati ◽  
Archana Shrivastav ◽  
◽  
◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Seed Protein ◽  
Growth Parameters ◽  
Saline Water ◽  
Protein Profile ◽  
Harmful Effect ◽  
Nacl Salinity ◽  
Sds Page ◽  
Overall Performance ◽  
Better Than

Soil salinity is one of the most severe factors limiting growth and physiological response in cowpea plants. In the present study, the effect of rhizobacterium strains BR2 and BR3 on the growth of cowpea (Vigna unguiculata L.) varieties—Pusa Sukomal and RC101—tolerance to 0, 25, 50, and 75 mM concentrations of NaCl salinity was evaluated. The rate of growth, in general, was high in plants irrigated with 25 mM NaCl saline water as compared to control, and thereafter, the growth reduced with increase in salinity concentrations. The results revealed that treating the seeds with rhizobacteria accompanied by NaCl salinity increased growth parameters of the cowpea plant as compared to the seeds irrigated with sodium chloride alone. Treatment with rhizobacteria mitigated the harmful effect of NaCl, and the growth was significantly better than the plants growing in saline water without rhizobacterium inoculation. The overall performance of Pusa Sukomal with BR3 strain was found to be better than the other combinations tested. Flowering in field plants started within 45 days of sowing, and the seeds in plants irrigated with saline water, in the presence of rhizobacterium, were found to be healthy as compared to control seeds. Seed protein profile was analyzed by SDS PAGE gel studies.

scholarly journals Comparative Analysis of Electrophoretic Profile of Major Proteins of Milk from Alpine and Carpathian Goats

2017 ◽  
Vol 74 (1) ◽  
pp. 20 ◽  
Author(s):  
Alina NASALEAN ◽  
Laurentiu OGNEAN ◽  
Sergiu MUNTEAN ◽  
Stefana BALICI ◽  
Horea MATEI
Keyword(s):  
Protein Profile ◽  
Milk Proteins ◽  
Goat Milk ◽  
Protein Composition ◽  
Raw Milk ◽  
Biologically Active ◽  
Protein Fractions ◽  
Animal Nutrition ◽  
Sds Page ◽  
Percentage Data

The milk’s proteins provide nutritional and biologically active values, essential in human and animal nutrition. In the case of goat milk, the proteins’ concentration and quality represent basic indices for the evaluation of the nutritional and biologically active values. The proposal is to comparatively analyse the protein profile of milk. The milk was collected from two different breeds: French Alpine and Romanian Carpathian. During March and April 2016 there were collected samples of raw milk in hygienic and sanitation conditions. There were two lots: first lot has 10 Carpathian goats and the second lot has 10 Alpine goats. The protein composition of goat milk was established with SDS-PAGE, after the evaluation of the total proteins’ concentration with the Bradford method. The quantitative and percentage data obtained with electrophoresis revealed few differences between those 8 identified protein fractions. Between those two lots, regarding the levels of β-CN, k-CN and β-lactoglobulines there were significant differences. The other protein fractions have values almost identical. Statistical analysis of obtained data shaped the differences in the protein profile at those two breeds. Based on those differences it is to note the superior potential of the Alpine breed regarding the content in biologically active milk proteins. Regarding the obtained data, this study brings new contributions for the evaluation and analysis of protein profile as a nutritive and biologically active component of goat milk, confirming its character as a functional aliment.

scholarly journals Differential Expression Gene/Protein Contribute to Heat Stress-Responsive in Tetraena propinqua in Saudi Arabia

2021 ◽  
Author(s):  
Hayat Ali Alafari ◽  
Magda Abdelgawad
Keyword(s):  
Heat Stress ◽  
Abiotic Stresses ◽  
Molecular Mechanisms ◽  
Natural Habitat ◽  
Protein Profile ◽  
Differential Regulation ◽  
Dual Specificity ◽  
Differential Expression Gene ◽  
Sds Page ◽  
Gene And Protein Expression

Abstract BackgroundWithin their natural habitat, plants are subjected to abiotic stresses that include heat stress. In the current study, the effect of 4h, 24h and 48h of heat stress on Tetraena propinqua ssp. migahidii seedling’s protein profile and proteomic analyses were investigated. ResultsTotal soluble protein SDS-PAGE profile showed 18-protein bands downregulated at 4h and 48h, however, 20-protein bands were upregulated at 24h of heat stress. A proteomic analysis showed that 81 and 59 targets are involved in gene and protein expression respectively. ConclusionsThe genes and proteins involved in transcription, translation, photosynthesis, transport and other unknown metabolic processes, were differentially expressed under treatments of heat stress. These findings provide insights into the molecular mechanisms related to heat stress, in addition to its influence on the physiological traits of T. propinqua seedlings. Heat stress mediated differential regulation genes indicate a role in development and stress response of T. propinqua. The candidate dual specificity genes identified in this study paves way for more molecular analysis of up- and down-regulation.

scholarly journals Stability evaluation of house dust mite vaccines for sublingual immunotherapy

2010 ◽  
Vol 75 (1) ◽  
pp. 19-26
Author(s):  
Lidija Burazer ◽  
Katarina Milovanovic ◽  
Tanja Cirkovic-Velickovic ◽  
Marija Gavrovic-Jankulovic
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Sublingual Immunotherapy ◽  
Protein Profile ◽  
Binding Potential ◽  
Allergen Specific Immunotherapy ◽  
Sds Page ◽  
Dust Mite ◽  
The Stability ◽  
Allergen Extracts

Allergen-specific immunotherapy with house dust mite (HDM) allergen extracts can effectively alleviate the symptoms of allergic rhinitis and asthma. The efficacy of the immunotherapeutic treatment is highly dependent on the quality of house dust mite vaccines. This study was performed to assess the stability of house dust mite allergen vaccines prepared for sublingual immunotherapy. Lyophilized Dermatophagoides pteronyssinus (Dpt) mite bodies were the starting material for the production of sublingual vaccines in four therapeutic concentrations. The stability of the extract for vaccine production, which was stored below 4 ?C for one month, showed consistence in the protein profile in SDS PAGE. ELISA-inhibition showed that the potencies of Dpt vaccines during a 12 month period were to 65-80 % preserved at all analyzed therapeutic concentrations. This study showed that glycerinated Dpt vaccines stored at 4?C preserved their IgE-binding potential during a 12 month period, implying their suitability for sublingual immunotherapeutic treatment of HDM allergy.

scholarly journals Phylogenetic relations in section Arachis based on seed protein profile

1991 ◽  
Vol 82 (5) ◽  
pp. 593-597 ◽  
Author(s):  
A. K. Singh ◽  
S. Sivaramakrishnan ◽  
Melak H. Mengesha ◽  
C. D. Ramaiah
Keyword(s):  
Seed Protein ◽  
Protein Profile ◽  
Phylogenetic Relations

Studies on the Rosaceae II- SDS-PAGE Seed Protein Electrophoresis and its Significance in the Taxonomy of the Family

2003 ◽  
Vol 6 (21) ◽  
pp. 1820-1829
Author(s):  
A.E. Dowidar ◽  
E.A. Kamel ◽  
A.M. Ahamed ◽  
M.H.A. Loutfy ◽  
H.H.L. Hafez
Keyword(s):  
Seed Protein ◽  
Protein Electrophoresis ◽  
Seed Protein Electrophoresis ◽  
Sds Page ◽  
The Family
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