Measurement of the isomer production ratio for the Cd112(n,γ)Cd113 reaction using neutron beams at J-PARC

2016 ◽  
Vol 94 (5) ◽  
Author(s):  
T. Hayakawa ◽  
Y. Toh ◽  
M. Huang ◽  
T. Shizuma ◽  
A. Kimura ◽  
...  
Keyword(s):  
Neutron Beams ◽  
Production Ratio

scholarly journals Ethanologenesis and respiration in a pyruvate decarboxylase-deficient Zymomonas mobilis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Reinis Rutkis ◽  
Inese Strazdina ◽  
Zane Lasa ◽  
Per Bruheim ◽  
Uldis Kalnenieks
Keyword(s):  
Parent Strain ◽  
Zymomonas Mobilis ◽  
Nadh Oxidase ◽  
Pyruvate Decarboxylase ◽  
Production Ratio ◽  
Pyruvate Node ◽  
Branching Point ◽  
Adh Activity ◽  
Ethanol Synthesis

Abstract Objective Zymomonas mobilis is an alpha-proteobacterium with a rapid ethanologenic pathway, involving Entner–Doudoroff (E–D) glycolysis, pyruvate decarboxylase (Pdc) and two alcohol dehydrogenase (ADH) isoenzymes. Pyruvate is the end-product of the E–D pathway and the substrate for Pdc. Construction and study of Pdc-deficient strains is of key importance for Z. mobilis metabolic engineering, because the pyruvate node represents the central branching point, most novel pathways divert from ethanol synthesis. In the present work, we examined the aerobic metabolism of a strain with partly inactivated Pdc. Results Relative to its parent strain the mutant produced more pyruvate. Yet, it also yielded more acetaldehyde, the product of the Pdc reaction and the substrate for ADH, although the bulk ADH activity was similar in both strains, while the Pdc activity in the mutant was reduced by half. Simulations with the kinetic model of Z. mobilis E-D pathway indicated that, for the observed acetaldehyde to ethanol production ratio in the mutant, the ratio between its respiratory NADH oxidase and ADH activities should be significantly higher, than the measured values. Implications of this finding for the directionality of the ADH isoenzyme operation in vivo and interactions between ADH and Pdc are discussed.

scholarly journals Improving the monacolin K to citrinin production ratio in red yeast rice by an X-ray-induced mutant strain of Monascus purpureus

2018 ◽  
Vol 99 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Apinun Kanpiengjai ◽  
Rapeepun Mahawan ◽  
Prodpran Pengnoi ◽  
Saisamorn Lumyong ◽  
Chartchai Khanongnuch
Keyword(s):  
Mutant Strain ◽  
Monascus Purpureus ◽  
Red Yeast Rice ◽  
Monacolin K ◽  
X Ray ◽  
Production Ratio ◽  
Red Yeast ◽  
Induced Mutant

Aerobic culture of Propionibacterium freudenreichii ET-3 can increase production ratio of 1,4-dihydroxy-2-naphthoic acid to menaquinone

2006 ◽  
Vol 101 (6) ◽  
pp. 464-470 ◽  
Author(s):  
Keisuke Furuichi ◽  
Ken-ichi Hojo ◽  
Yoshio Katakura ◽  
Kazuaki Ninomiya ◽  
Suteaki Shioya
Keyword(s):  
Propionibacterium Freudenreichii ◽  
Aerobic Culture ◽  
Production Ratio ◽  
Naphthoic Acid
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