Aerobic culture of Propionibacterium freudenreichii ET-3 can increase production ratio of 1,4-dihydroxy-2-naphthoic acid to menaquinone

2006 ◽  
Vol 101 (6) ◽  
pp. 464-470 ◽  
Author(s):  
Keisuke Furuichi ◽  
Ken-ichi Hojo ◽  
Yoshio Katakura ◽  
Kazuaki Ninomiya ◽  
Suteaki Shioya
Keyword(s):  
Propionibacterium Freudenreichii ◽  
Aerobic Culture ◽  
Production Ratio ◽  
Naphthoic Acid

scholarly journals Enhancement of 1,4-Dihydroxy-2-Naphthoic Acid Production by Propionibacterium freudenreichii ET-3 Fed-Batch Culture

2007 ◽  
Vol 73 (10) ◽  
pp. 3137-3143 ◽  
Author(s):  
Keisuke Furuichi ◽  
Yoshio Katakura ◽  
Kazuaki Ninomiya ◽  
Suteaki Shioya
Keyword(s):  
Batch Culture ◽  
Feeding Rate ◽  
Propionibacterium Freudenreichii ◽  
Fed Batch ◽  
Aerobic Culture ◽  
Naphthoic Acid ◽  
Lactose Concentration ◽  
Fed Batch Culture ◽  
Source Limitation ◽  
Cultivation Experiment

ABSTRACT The production of 1,4-dihydroxy-2-naphthoic acid (DHNA) was investigated using a fed-batch culture of Propionibacterium freudenreichii ET-3. DHNA is a precursor of menaquinone (MK) and is transformed to MK by combination with an isoprenoid unit. We found that ET-3 stopped MK production and increased DHNA production in an anaerobic fed-batch culture by maintaining the lactose concentration at approximately zero. The maximum DHNA concentration observed in the anaerobic fed-batch culture was markedly higher than the maximum DHNA concentration observed in an anaerobic batch culture. Moreover, MK or DHNA production was affected by the lactose feeding rate; this suggests that lactose metabolism participates in the syntheses of these products. On the other hand, accumulation of propionate was found to inhibit DHNA production in the fed-batch culture. Based on the fact that ET-3 increases DHNA production in an aerobic culture by consuming propionate, we carried out a cultivation experiment in which an anaerobic fed-batch culture was switched to an anaerobic batch culture and found that the DHNA production was increased to a greater extent than the DHNA production in an anaerobic fed-batch culture. These results suggest that DHNA production by ET-3 is markedly influenced by carbon source limitation and the oxygen supply.

Optimal aerobic cultivation method for 1,4-dihydroxy-2-naphthoic acid production by Propionibacterium freudenreichii ET-3

2006 ◽  
Vol 102 (3) ◽  
pp. 198-205 ◽  
Author(s):  
Keisuke Furuichi ◽  
Atsuko Amano ◽  
Yoshio Katakura ◽  
Kazuaki Ninomiya ◽  
Suteaki Shioya
Keyword(s):  
Acid Production ◽  
Propionibacterium Freudenreichii ◽  
Cultivation Method ◽  
Naphthoic Acid ◽  
Aerobic Cultivation

Pulse polarographic study of the behaviour of some o,o'-dihydroxyazo-compounds

1989 ◽  
Vol 54 (5) ◽  
pp. 1219-1226 ◽  
Author(s):  
Enric Casassas ◽  
Miquel Esteban ◽  
Santiago Alier
Keyword(s):  
Carboxylic Acid ◽  
Reaction Mechanisms ◽  
Sulphonic Acid ◽  
Base Catalysis ◽  
Polarographic Study ◽  
Acid Base ◽  
Eriochrome Black T ◽  
Naphthoic Acid ◽  
Calcon Carboxylic Acid

The reduction of several o,o'-dihydroxyazo-compounds is studied by means of pulse polarographic techniques (DPP, NPP and RPP). The compounds studied are the following: 2-(2'-hydroxyphenylazo)-phenol (o,o'-dihydroxyazobenzene), 1-(2'-hydroxy-1'-naphthylazo)-2-naphthol-4-sulphonic acid (calcon or Eriochrome Blue Black R), 1-(2'-hydroxy-4'-sulpho-1'-naphthylazo)-2-hydroxy-3-naphthoic acid (calcon carboxylic acid), and 1-(1'-hydroxy-2'-naphthylazo)-6-nitro-2-naphthol-4-sulphonic acid (Eriochrome Black T). Correlations between Ip and Epand experimental variables (pH, T, conc.) and instrumental parameters (dropping time, t, and pulse magnitude, ΔE) are established. Reaction mechanisms formerly proposed are discussed on the basis of the new obtained results, and the ranges are defined where adsorption and/or acid-base catalysis are operative.

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