scholarly journals Microbial Transport as Affected by Residue Cover and Manure Application Rate

2019 ◽  
Vol 62 (3) ◽  
pp. 687-694
Author(s):  
Lisa A. Durso ◽  
John E. Gilley ◽  
David B. Marx ◽  
Chance A. Thayer ◽  
Bryan L. Woodbury
Keyword(s):  
Total Coliform ◽  
Application Rate ◽  
Land Application ◽  
Rainfall Simulation ◽  
Nutrient Requirements ◽  
Microbial Transport ◽  
Manure Application ◽  
Rainfall Events ◽  
Total Coliforms ◽  
E Coli

Abstract. Manure is applied to cropland areas with varying surface cover to meet single- or multiple-year crop nutrient requirements. The objectives of this field study were to (1) examine microbial transport following land application of manure to sites with and without wheat residue, (2) compare microbial loads following land application to meet the 0, 1, 2, 4, and 8-year P-based requirements for corn, and (3) evaluate the effects of rainfall simulation run on microbial transport. Manure was added and incorporated by disking plots that were 0.75 m wide by 2.0 m long. Three 30 min simulated rainfall events, separated by 24 h intervals, were then applied at an intensity of 70 mm h-1. Plots containing wheat residue had a total coliform load of 12.6 log CFU ha-1, which was significantly greater than the 12.4 log CFU ha-1 measured on the plots without wheat residue. The plots with and without wheat residue had transport rates of and enterococci that were not significantly different. The plots on which manure was added at rates varying from 5.4 to 42.8 Mg ha-1 had counts of total coliforms and enterococci that were not significantly different. Rainfall simulation run did not significantly affect measurements of phages, total coliforms, or enterococci. Transport of selected microbes was found to be significantly affected by residue cover, manure application rate, and rainfall simulation run. Keywords: Bacteria, Cattle manure, E. coli, Feedlots, Land application, Manure management, Manure runoff, Microbial, Microorganisms, Runoff.

scholarly journals Narrow Grass Hedge Effects on Microbial Transport Following Variable Applications of Beef Cattle Manure

2019 ◽  
Vol 62 (1) ◽  
pp. 149-156 ◽  
Author(s):  
Lisa A. Durso ◽  
John E. Gilley ◽  
David B. Marx ◽  
Bryan L. Woodbury
Keyword(s):  
Beef Cattle ◽  
Land Application ◽  
Cattle Manure ◽  
Rainfall Simulation ◽  
Silty Clay ◽  
Microbial Transport ◽  
Manure Application ◽  
Total N ◽  
Total Coliforms ◽  
Application Rates

Abstract. The effectiveness of a 1.4 m wide grass hedge in reducing microbial transport following manure application was examined in this study. Beef cattle manure was applied to 0.75 m wide by 4.0 m long plots established on an Aksarben silty clay loam located in southeast Nebraska. Manure was added at rates required to meet none or the 1-, 2-, or 4-year nitrogen requirements for corn. The transport of phages, total coliforms, , and enterococci was measured for three 30 min simulated rainfall events, which were separated by approximately 24 h intervals. The narrow grass hedge reduced total counts of phages, , and enterococci from 10.8 to 9.01 log PFU ha-1, from 12.4 to 11.9 log CFU ha-1, and from 11.8 to 11.2 log CFU ha-1, respectively. For the plots that received manure, no significant differences in transport of phages or enterococci were found among the three manure application rates. Rainfall simulation run significantly affected measurements of phages, total coliforms, and enterococci, with measurements during the three runs varying from 8.91 to 10.5 log PFU ha-1, from 12.7 to 13.3 log CFU ha-1, and from 11.2 to 11.7 log CFU ha-1, respectively. Counts for phages, total coliforms, and enterococci were significantly less for the first than the second and third rainfall simulation runs. All four of the microbial constituents were significantly correlated to dissolved P, particulate P, total P, and total N. A narrow grass hedge placed on the contour significantly reduced microbial transport following variable applications of beef cattle manure. Keywords: Bacteria, Cattle manure, E. coli, Filter strips, Land application, Manure management, Manure runoff, Microbial, Microorganisms, Runoff.

scholarly journals Effects of Animal Diet, Manure Application Rate, and Tillage on Transport of Microorganisms from Manure-Amended Fields

2011 ◽  
Vol 77 (18) ◽  
pp. 6715-6717 ◽  
Author(s):  
Lisa M. Durso ◽  
John E. Gilley ◽  
David B. Marx ◽  
Bryan L. Woodbury
Keyword(s):  
Application Rate ◽  
Rainfall Simulation ◽  
Bacterial Transport ◽  
Microbial Transport ◽  
Manure Application ◽  
No Till ◽  
Distillers Grain ◽  
Animal Diet

ABSTRACTManure from cattle fed distillers' grain or corn diets was applied to fields, and the fields were subjected to rainfall simulation tests. Manure was added at three rates on till and no-till plots. Correlations between microbial transport and runoff characteristics were identified. Results indicate that diet affects phage but not bacterial transport from manure-amended fields.

Influence of long-term manure application on mineral composition of irrigated barley silage

2015 ◽  
Vol 95 (4) ◽  
pp. 759-770 ◽  
Author(s):  
J. J. Miller ◽  
B. W. Beasley ◽  
C. F. Drury ◽  
F. J. Larney ◽  
X. Hao
Keyword(s):  
Mineral Composition ◽  
Application Rate ◽  
Land Application ◽  
Wood Chips ◽  
Fertilizer Treatment ◽  
Manure Application ◽  
Feed Quality ◽  
Barley Silage

Miller, J. J., Beasley, B. W., Drury, C. F., Larney, F. and Hao, X. 2015. Influence of long-term manure application on mineral composition of irrigated barley silage. Can. J. Plant Sci. 95: 759–770. The long-term effect of land application of manure type (composted vs. stockpiled manure), bedding type (wood-chips vs. straw), and application rate on feed quality of barley silage as feed for beef cattle is unknown. We measured selected minerals [P, Ca, Ca:P ratio, Mg, K, K:(Ca+Mg) ratio, Na, Fe, Mn, Zn, Cu] of irrigated barley silage (Hordeum vulgare L.) on a clay loam soil after 4 (2002), 7 (2005) and 11 (2009) years of annual applications of composted (CM) or stockpiled (SM) feedlot manure with wood-chips (WD) or straw (ST) bedding at three application rates (13, 39, 77Mg ha−1 dry wt.). The treatments also included an unamended control and inorganic fertilizer treatment. Manure type generally had inconsistent or no significant (P≤0.05) effect on the concentrations of these minerals in barley silage. Most crop minerals were generally greater under ST than WD. The findings for P, K, Na, and K:(Ca+Mg) ratio generally supported our hypothesis of greater crop concentrations with greater application rate, but Ca and Mg decreased at higher rates. Overall, our findings suggest that bedding and application rate have more potential than manure type for managing the feed quality of barley silage.

Effects of rainfall on the occurrence of human adenoviruses, total coliforms, and Escherichia coli in seawater

2006 ◽  
Vol 54 (3) ◽  
pp. 225-230 ◽  
Author(s):  
E. Haramoto ◽  
H. Katayama ◽  
K. Oguma ◽  
Y. Koibuchi ◽  
H. Furumai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Real Time Pcr ◽  
Tokyo Bay ◽  
Rainfall Events ◽  
Total Coliforms ◽  
The Real ◽  
E Coli ◽  
Human Adenoviruses ◽  
Seawater Samples

A two-month survey was conducted in order to evaluate the effects of rainfall on the fate of microorganisms in seawater in the Tokyo Bay, Japan. The seawater sample (1,000 mL) was applied to a method to concentrate virus, followed by a quantification of human adenoviruses using the real-time PCR. Total coliforms and E. coli, which were determined by the colony forming method, were detected in all 47 seawater samples, while human adenoviruses were detected in 38 (81%) of the samples. The concentration of tested microorganisms showed 1–2 log units increase after rainfall events, followed by the gradual decrease to the level before the rainfall within a few days.

ColiComplete® Substrate-Supporting Disc Method for Confirmed Detection of Total Coliforms and Escherichia coli in All Foods: Comparative Study

1994 ◽  
Vol 77 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Philip T Feldsine ◽  
Maria T Falbo-Nelson ◽  
David L Hustead
Keyword(s):  
Escherichia Coli ◽  
Comparative Study ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Total Coliforms ◽  
E Coli ◽  
Total Coliform Bacteria ◽  
Better Than

Abstract The ColiComplete® substrate-supporting disc (SSD) method for simultaneous confirmed total coliform count and Escherichia coli determination in all foods was compared with the AOAC most probable number (MPN) methods 966.23 and 966.24. In this comparative study, 20 water and food types were analyzed; 7 of these foods were naturally contaminated with coliform bacteria, 6 food types were naturally contaminated with E. coli, and the remaining foods were inoculated with coliform bacteria and/or E. coli. Data were analyzed separately for total coliform bacteria and for E. coli. Mean log MPN counts were determined by the SSD method and the appropriate AOAC MPN procedure. Results were then analyzed for mean log MPN differences and variance, according to methods described by AOAC INTERNATIONAL Results for both total conforms and E. coli indicate that the SSD method is equivalent to or better than AOAC MPN methods 966.23 and 966.24.

Prevalence of Escherichia coli O157:H7 and Indicator Organisms on the Surface of Intact Subprimal Beef Cuts Prior to Further Processing

2006 ◽  
Vol 69 (7) ◽  
pp. 1514-1517 ◽  
Author(s):  
JAMES E. KENNEDY ◽  
SALLY K. WILLIAMS ◽  
TED BROWN ◽  
PHIL MINERICH
Keyword(s):  
Escherichia Coli ◽  
Total Coliform ◽  
Escherichia Coli O157 ◽  
Indicator Organisms ◽  
Seasonal Differences ◽  
Total Coliforms ◽  
E Coli ◽  
Plate Counts ◽  
Collection Period

The objective of this study was to determine the prevalence of Escherichia coli O157:H7, other E. coli strains, total coliforms, and aerobic organisms on the surface of subprimal beef cuts prior to enhancement. Subprimal cuts were sampled during winter (January and February 2004) and summer (August through October 2004). During each collection period, six representative subprimal cuts (chuck tenders, 0.64-cm trimmed strips, bottom round flat, rough-trimmed brisket, cap-on top rounds, and cap-off insides) were sampled. A total of 600 samples in winter (100 samples per cut) and 599 samples in summer (100 chuck tenders, 100 0.64-cm trimmed strips, 100 bottom round flats, 100 cap-off insides, 97 rough-trimmed briskets, and 102 cap-on top rounds) were collected from five plants in the Midwest, southern Midwest, northern Midwest, and Southeast and swabbed using the sponge swab method. All sponges were analyzed for E. coli O157:H7. In addition, 400 subprimal cuts from four plants were analyzed for aerobic plate counts, total coliforms, and other E. coli strains during each collection period. E. coli O157:H7 was not detected on any of the 1,199 subprimal samples; thus, incidence of E. coli O157:H7 was <0.083%. Seasonal differences between aerobic plate counts and total coliform counts for each of the same cuts were 1.0 log CFU per cut or less. E. coli strains were not detected in 82, 52, 69, and 82% of the chuck tenders, 0.64-cm trimmed strips, bottom round flats, and cap-off insides, respectively.

Comparative performance of colisureTM and accepted methods in the detection of chlorine-injured total coliforms and E. coli

1995 ◽  
Vol 31 (5-6) ◽  
pp. 259-261
Author(s):  
G. A. McFeters ◽  
S. C. Broadaway ◽  
B. H. Pyle ◽  
M. Pickett ◽  
Y. Egozy
Keyword(s):  
Drinking Water ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Comparative Performance ◽  
Total Coliforms ◽  
E Coli ◽  
Reference Methods ◽  
The Us ◽  
Viable Bacteria ◽  
Contaminated Drinking Water

Studies were done to examine the comparability of ColisureTM and accepted reference methods to detect low numbers of total coliform bacteria and E. coli subjected to chlorine stress. ColisureTM is a medium designed to concurrently detect coliform bacteria and E. coli in drinking water by the specific action of β-galactosidase (total coliforms) and β-glucuronidase (E. coli). The methods used to compare the performance of various media followed a protocol established by the USEPA. Samples (31) of sewage from six different regions of the US were treated with sufficient concentrations of chlorine (1.2-2.5mg/l) to reduce viability 1-3 logs (39% average injury) and diluted with drinking water to achieve ca. 3 viable coliforms/100ml. The mean log reductions in viable bacteria, determined with various media following disinfection of the 31 samples were: mEndo = 1.87 (TC), ColisureTM = 1.55 (TC), mTec = 3.63 (E. coli) and ColisureTM = 2.01 (E. coli). When ColisureTM was compared with accepted methods to detect total coliforms in the dilute, disinfected samples, ColisureTM yielded results that were 1.6 times greater than LTB confirmed in BGLB at 28h. ColisureTM also detected 1.7 times greater levels of E. coli than LTB confirmed in EC/MUG at 28h. Sensitivity and specificity of ColisureTM were between 96 and 100% when positive and negative tests were verified. These findings indicate that ColisureTM is superior to certain accepted reference methods in the detection of chlorine-injured coliforms and E. coli under conditions that resemble contaminated drinking water.

E. coli from dishcloths as an indicator of hygienic status in households

2015 ◽  
Vol 5 (3) ◽  
pp. 351-358 ◽  
Author(s):  
V. Keshav ◽  
C. A. Kruger ◽  
A. Mathee ◽  
N. Naicker ◽  
A. Swart ◽  
...  
Keyword(s):  
South Africa ◽  
Public Education ◽  
Model Organism ◽  
Total Coliform ◽  
Fecal Pollution ◽  
Total Coliforms ◽  
E Coli ◽  
Study Results ◽  
Enrichment Step

E. coli is routinely used as an indicator of fecal pollution, although some strains are capable of causing diarrhea. E. coli was used as a model organism for this study to assess the possibility that dishcloths used in households could contribute to the occurrence of diarrhea. Dishcloths (n = 424) were collected from five suburbs in Johannesburg (South Africa) as part of a larger Health, Environment & Development (HEAD) study. Results for the total coliforms indicated that on average 81% of the samples analyzed had total coliform counts of more than 1,000 cfu/100 ml per 25 cm2 cloth. The E. coli results indicated that 40% of the samples had culturable E. coli present with 17% of the samples showing the presence of >1,000 cfu/100 ml per 25 cm2 cloth. Except for the samples from Bertrams, all the pathogenic E. coli genes could be detected in various combinations in the different samples. Since all the diarrheagenic E. coli strains detected can be accepted as culturable due to the enrichment step, there is a clear danger of contamination of food and surfaces exposed to the contaminated dishcloths. The results indicated that there is a need for public education regarding hygiene in the households, especially if the same dishcloth is used for various tasks.

scholarly journals Sampling of elementary school playground equipment for total coliforms and E. coli

2016 ◽  
Author(s):  
David Fowler ◽  
BCIT School of Health Sciences, Environmental Health ◽  
Vanessa Karakilic
Keyword(s):  
Elementary School ◽  
School Setting ◽  
Total Coliform ◽  
P Value ◽  
Test Analysis ◽  
Total Coliforms ◽  
E Coli ◽  
The Mean ◽  
Contact Surfaces ◽  
Hand Contact

  Background and Purpose: Outbreaks of enteric diseases in schools and daycares are common. It is possible that these outbreaks could be propagated via fomites in school settings, such as playground equipment that is not regularly cleaned. Studies thus far have provided conflicting results on the level of contamination present on fomites in the school setting. This project is intended to assess the level of microbial contamination present on elementary school playground surfaces as a result of hand contact from school children. Methods: Two categories of elementary school playground equipment were sampled in this study; those that are likely to see regular hand contact from children and those that were not likely see hand regular hand contact from children. 30 surfaces of each category were swabbed and the media will be plated and incubated to enumerate total coliforms and E. coli. Results: The mean number of total coliforms on high hand contact surfaces was 0.2333 cfu/100cm2, while the mean number of total coliforms on low hand contact surfaces was 0.2667 cfu/100cm2. The t-test analysis of total coliform results produced a p-value of 0.5566. The mean number of E. coli on high hand contact surfaces was 1.1333 cfu/100cm2, while the mean number of E. coli on low hand contact surfaces was 4.9000 cfu/100cm2. The t-test analysis of E. coli results produced a p-value of 0.8019. Discussion: Neither results for total coliforms or E. coli indicated significantly different numbers on high or low hand contact surfaces. This indicates that these total coliform and E. coli may not be present as a result of hand contact. Conclusion: These results do not support the deposition of coliforms or E. coli on playground equipment as a result of hand contact. However, there is still concern due to the number of samples positive for E. coli. These results suggest the need for practices such as regular hand washing in the school setting after using the playground, regular cleaning of playground materials and EHO inspections of school grounds.  

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