scholarly journals Narasin as a Manure Additive to Reduce Methane Production from Swine Manure

2018 ◽  
Vol 61 (3) ◽  
pp. 943-953
Author(s):  
Daniel S. Andersen ◽  
Fan Yang ◽  
Steven L. Trabue ◽  
Brian J. Kerr ◽  
Adina Howe
Keyword(s):  
Methane Production ◽  
Biogas Production ◽  
Safety Concern ◽  
Swine Manure ◽  
Inhibitory Effect ◽  
Gas Production ◽  
Dose Rates ◽  
Deep Pit ◽  
Sugar Addition ◽  
Swine Operations

Abstract. High levels of methane production from swine operations have been associated with foam accumulation in deep-pit manure storage systems. This foam poses both a safety concern (i.e., flash fires) and operational challenges in managing stored manure. Mitigating methane production is one approach to controlling foam accumulation. In this study, swine manures obtained from three deep-pit storage barns in central Iowa were dosed with narasin to evaluate its inhibitory effects on methane and biogas production. Dose rates ranged from 0 to 3.0 mg narasin kg-1 manure. Overall, methane rates were reduced by 9% for each mg of narasin added per kg of manure, and this reduction was effective for up to 25 days. However, the inhibitory effect weakened with time such that no statistical difference in cumulative methane production between samples dosed with narasin and undosed controls could be detected after 120 days of incubation. In addition to methane rates, narasin addition reduced the degradation of total and volatile solids in the manure by 1.9% and 2.6%, respectively, for each mg of narasin added per kg of manure. Additional study treatments included sugar (10 g kg-1 manure) with and without narasin (1.5 mg narasin kg-1 manure). Results from this treatment showed that methane production was initially increased by the sugar addition, but the effect lasted less than six days, at which point cumulative methane production was similar to the control. When treated with both narasin and sugar, the inhibitory effect did not impact gas production during the sugar digestion phase but did reduce methane and biogas production thereafter. The addition of sugar and the rate of narasin addition caused changes to the microbial community as compared to the control. Overall, the results indicated that narasin can be an effective additive for reducing methane emission from swine manure, but further study is needed to recommend dosing frequency and to evaluate how continuous addition of manure impacts narasin effectiveness. Keywords: Biogas, Manure management, Manure treatment, Methane, Narasin, Swine manure, Swine production.

Study of Methane Production by High Temperature Anaerobic Fermentation of Chicken Manure and Stalks

2020 ◽  
Vol 14 (4) ◽  
pp. 551-557
Author(s):  
Yongku Li ◽  
Xiaomin Hu ◽  
Lei Feng
Keyword(s):  
High Temperature ◽  
Anaerobic Digestion ◽  
Production Rate ◽  
Methane Production ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Gas Production ◽  
Chicken Manure ◽  
Biogas Slurry ◽  
Production Rates

The changing parameters, as the biogas production rate, the methane production rate, the cumulative biogas amount, the cumulative methane amount, the biogas composition, pH etc. in high temperature anaerobic fermentation of chicken manure and stalks were analyzed by experiments with different mass ratios of chicken manure or livestock manure and stalks with a high C/N ratio. The methane production mechanism of high temperature anaerobic digestion of chicken manure and stalks was discussed in detail. It showed that not only the biogas production rates but also the methane production rates of R1–R7 demonstrated the trend of initial increase and then decrease after 50 d of high temperature anaerobic digestion. Besides, the gas production of R1 with pure chicken manure stopped on the 30th d of the reaction. The gas production of other groups R2–R7 also stopped on the corresponding 34th, 36th, 36th, 37th, 37th, and 37th day, respectively. At the end of the reaction, the cumulative biogas amounts and the cumulative methane amounts of R1–R7 were 411.58 and 269.54, 459.91 and 314.41, 425.32 and 294.11, 401.85 and 272.54, 382.63 and 257.07, 363.04 and 218.16, and 257.15 and 160.10 N ml/(g VS). The biogas slurry pH of R1–R7 all demonstrated a trend of initial decrease and then increase, e. g., pH of R2 reached the minimum of 5.94 on the 5th day. pH values of other groups were between 6.01 and 6.39. After the addition of 4 g of sodium bicarbonate on the 7th day, biogas slurry pH of R1–R7 all increased. pH was maintained between 7.16 and 7.44 until the end of the reaction.

scholarly journals Aeration to Improve Biogas Production by Recalcitrant Feedstock

Environments ◽  
2019 ◽  
Vol 6 (4) ◽  
pp. 44 ◽  
Author(s):  
John Loughrin ◽  
Nanh Lovanh
Keyword(s):  
Carbon Dioxide ◽  
Anaerobic Digestion ◽  
Methane Production ◽  
Biogas Production ◽  
Day Treatment ◽  
Poultry Litter ◽  
Gas Production ◽  
Quality Degradation ◽  
Small Scales ◽  
Low Levels

Digestion of wastes to produce biogas is complicated by poor degradation of feedstocks. Research has shown that waste digestion can be enhanced by the addition of low levels of aeration without harming the microbes responsible for methane production. This research has been done at small scales and without provision to retain the aeration in the digestate. In this paper, low levels of aeration were provided to poultry litter slurry through a sub-surface manifold that retained air in the sludge. Digestate (133 L) was supplied 0, 200, 800, or 2000 mL/day air in 200 mL increments throughout the day via a manifold with a volume of 380 mL. Digesters were fed 400 g of poultry litter once weekly until day 84 and then 600 g thereafter. Aeration at 200 and 800 mL/day increased biogas production by 14 and 73% compared to anaerobic digestion while aeration at 2000 mL/day decreased biogas production by 19%. Biogas quality was similar in all digesters albeit carbon dioxide and methane were lowest in the 2000 mL/day treatment. Increasing feed to 600 g/week decreased gas production without affecting biogas quality. Degradation of wood disks placed within the digesters was enhanced by aeration.

Continuous biogas production from fodder beet silage as sole substrate

2003 ◽  
Vol 48 (4) ◽  
pp. 229-233 ◽  
Author(s):  
P.A. Scherer ◽  
S. Dobler ◽  
S. Rohardt ◽  
R. Loock ◽  
B. Büttner ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Swine Manure ◽  
Gas Production ◽  
Methane Content ◽  
Organic Loading Rate ◽  
Feeding Time ◽  
Reactor Performance ◽  
Fodder Beet ◽  
Gas Volume

Since April 2000 a two-step anaerobic plant with two subsequent 500 m3 reactors has been producing biogas from fodder beet silage (pH 4.1) as the sole substrate. The plant is located at Kirchlengern near Bielefeld, Germany. Initially the reactors were inoculated with swine manure at 37°C. After a start-up phase the process was sustained at pH 7.5-8.0 by feeding with the silage as sole substrate twice a day. Parallel to the biogas plant at Kirchlengern four one-step laboratory reactors were continuously driven at temperatures of 37°C, 45°C, 60°C and 65°C. They were fed with the same silage, but only once per day (one impulse). The organic loading rate (OLR) was adjusted to 3.9 g volatile solids (VS)/(l*d) with a concomitant hydraulic retention time (HRT) of 27 d. There was no problem with starting the reactors, but after 86 days the volumetric gas production of the 65°C reactor ceased and a high amount of approximately 130 mM propionate could be determined. By decreasing the temperature down to 60°C a stable reactor performance was recovered for a period of at least 250 further days. During impulse feeding it was observed that the quickest recovery of gas production could be observed at 37°C or at 45°C. Recovery of 75% gas volume (related to the value before or after impulse feeding) was obtained after 5.5 and 7.5 h of feeding time point whereas the 60°C reactor needed 16 h. Slight significant differences were seen in the spectrum of volatile fatty acids (VFA) reaching at 37° or 45°C its maximum with 10-30 mM total VFA at 2-3 h after feeding. After this the VFA level declined to nearly zero (except for the 60°C reactor). Therefore the 37°C reactor was favoured. A double experiment with a second 37°C reactor was started by a somewhat different inoculation procedure from the remaining 3 reactors, but revealed similar results. By increasing the temperature no significantly different specific gas production rates and methane yields could be observed, e.g. it gave 600-700 l biogas from 1 kg VS. The corresponding methane content ranged between 62-64%. With a methane content of 63 ± 1% a yield of 40.1 ± 2 m3 methane/ton fresh fodder beet silage was obtained.

Performance Evaluation of a Large-Scale Swine Manure Mesophilic Biogas Plant in China

2017 ◽  
Vol 60 (5) ◽  
pp. 1713-1720 ◽  
Author(s):  
Yi Wang ◽  
Wanqin Zhang ◽  
Hongmin Dong ◽  
Zhiping Zhu ◽  
Baoming Li
Keyword(s):  
Methane Production ◽  
Large Scale ◽  
Flow Reactor ◽  
Biogas Production ◽  
Plug Flow ◽  
Swine Manure ◽  
Energy System ◽  
Biogas Plant ◽  
Waste To Energy ◽  
Organic Loading Rate

Abstract. With the rapid growth of large-scale and intensive swine farms have come many ecological and environmental problems associated with the substantially increased and concentrated animal waste production. In this article, a swine manure and flushed slurry to renewable energy management system is present and discussed. This system was installed in a commercial feeder-to-finish swine farm with 18,000 head of swine in Beijing, China, and included two mesophilic upflow solids reactors (USRI and USRII, 500 m3 and 700 m3) and one psychrophilic plug-flow reactor (PFR, 1000 m3). In this study, USRII was monitored throughout a whole year to evaluate the performance of this swine waste to energy system. The biogas plant used mixed solid swine manure and flushed slurry as substrate with a relatively low organic loading rate (OLR) of 0.7 to 1.8 kg volatile solids (VS) m-3 d-1. The hydraulic retention time (HRT) varied from 15 to 22 days depending on the season. Less added water contributed to the longer HRT and more concentrated influent in winter. In winter, the specific methane production (SMP) of the digester was 0.43 m3 CH4 kg-1 VSadded, which was slightly lower than the value reported in Europe (0.45 m3 CH4 kg-1 VSadded) but about 48.3% higher than that in Asia (0.29 m3 CH4 kg-1 VSadded). This indicated that the performance of this USR in winter was stable, with a higher biogas production, and up to 90% of the VS was removed as well. However, the low OLR limited the volumetric methane production rate to only 0.21 to 0.57 m3 m-3 d-1. Keywords: Flushed slurry, Large-scale biogas plant, Monitoring, Performance, Swine manure.

scholarly journals Optimization of Hydrolysis-Acidogenesis Phase of Swine Manure for Biogas Production Using Two-Stage Anaerobic Fermentation

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1324
Author(s):  
Chiu-Yue Lin ◽  
Wai Siong Chai ◽  
Chyi-How Lay ◽  
Chin-Chao Chen ◽  
Chun-Yi Lee ◽  
...  
Keyword(s):  
Methane Production ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Swine Manure ◽  
Organic Loading Rate ◽  
Pig Manure ◽  
Volatile Acid ◽  
Two Stage ◽  
Batch Operation ◽  
Stage System

The traditional pig manure wastewater treatment in Taiwan has been low in methane production efficiency due to unstable influent concentration, wastewater volume, and quality. Two-stage anaerobic systems, in contrast, have the advantage of buffering the organic loading rate in the first stage (hydrolysis-acidogenesis phase), allowing a more constant feeding rate to the second stage (methanogenesis phase). Response surface methodology was applied to optimize the operational period (0.5–2.0 d) and initial operational pH (4–10) for hydrolysis and acidogenesis of the swine manure (total solid 5.3%) at 35 °C in batch operation mode. A methanogenesis verification experiment with the optimal condition of operational period 1.5 d and pH 6.5 using batch operation resulted in peak volatile acid production 7 g COD/L, methane production rate (MPR) 0.3 L-CH4/L-d, and methane yield (MY) 92 mL-CH4/g-CODre (chemical oxygen demand removed). Moreover, a two-stage system including a hydrolysis-acidogenesis reactor with the optimal operating condition and a methanogenesis reactor provided an average MPR 163 mL/L-d and MY 38 mL/g volatile solids, which values are 60% higher than those of a single-stage system; both systems have similar dominant methane-producing species of Firmicutes and Bacteroidetes with each having around 30%–40%. The advantages of a two-stage anaerobic fermentation system in treating swine manure for biogas production are obvious.

scholarly journals Application of BS4-enzyme on the methane production from mixture of cattle manures and waste paper

2018 ◽  
Vol 21 (4) ◽  
pp. 238
Author(s):  
Tuti Haryati ◽  
A. P. Sinurat ◽  
B. Listian ◽  
H. Hamid ◽  
T. Purwadaria
Keyword(s):  
Incubation Time ◽  
Methane Production ◽  
Dry Matter ◽  
Transformation Efficiency ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Gas Production ◽  
Waste Paper ◽  
Randomized Design ◽  
Completely Randomized Design

<p class="abstrak2">Cellulose from abundant newspaper waste could be transformed into methane through anaerobic fermentation. This research was carried out to compare the gas production including methane between samples containing feces and waste paper mixture as inoculum and substrate, respectively and added with and without BS4 enzyme. The enzyme was produced in Indonesian Research Institute of Animal Produce (IRIAP) by growing Eupenicillium javanicum BS4 in coconut meals. There were three treatments,  i.e., 30% manure (M30); 15 %  manure + 15 % paper waste  (MP 30); MP30 + 3 mL BS4 enzyme equal to 0.42 U/g dry matter (MPE30) The percentage of waste papers addition in feces was calculated on dry matter (DM) basis and every treatment had five replications. Total gas and methane productions were measured weekly, while dry matter losses were determined during 5 week fermentation. Interactions between treatments and incubation time were analyzed using completely randomized design each week. Kind of substrates influenced both total gas and methane productions during incubation time. Both waste papers and enzyme addition enhanced gas production. The highest total gas and methane productions for five weeks incubation were highly significantly observed (P&lt;0.01) in MP30 and MPE30 compared to M30. Addition of enzyme significantly increased total gas and methane productions in the first week. The highest methane and total gas yield/g dry matter were obtained by BS4 enzyme addition. It was concluded that BS4 enzyme is good in accelerating and increasing the transformation efficiency of waste paper and manure mixture for biogas production.</p><p><strong>Key Words</strong>: Methane, Fibrenolytic-Enzyme, Waste Papers, Cattle Manures</p>

Biogas Production from Swine Manure Co-Digestion with Hyacinth

2014 ◽  
Vol 953-954 ◽  
pp. 304-308
Author(s):  
Sorrasak Thayai ◽  
Supawat Vivanpatarakij
Keyword(s):  
Carbon Dioxide ◽  
Biogas Production ◽  
Swine Manure ◽  
Carbon Dioxide Production ◽  
Gas Production ◽  
Anaerobic Digesters ◽  
Mixing Ratios ◽  
Total Percentage ◽  
Pig Farm ◽  
Maximum Quantity

The suitable ratio between swine manure and hyacinth for biogas production was considered. From Ratchaburi Province, the swine manure and hyacinth were taken from the pig farm and natural canal, respectively. For this study, mixing ratios between swine manure with hyacinth are 0:100, 25:75, 50:50, 75:25 and 100:0, considered by dry basis. The anaerobic digesters were studied total mixing volume 300 ml in 500 ml volumetric flask on shaker. Total dry substrate is 2 g every ratio, swine manure and hyacinth. And microbial inoculum from pig farm is 3 g (dry basis). The experimental results showed the proportion of 0:100 illustrate the maximum quantity of biogas accumulated equal 243.48 ml. And the ratio of 100:0 shows the minimum cumulative biogas volume equal 33.60 ml. The analysis of the gas production, the blend of swine manure per hyacinth has the highest percentage of methane ratio is 0:100 (CH4=6.4%), and the lowest percentage is the ratio of 100:0 (CH4=0.5 %). For carbon dioxide production, the highest percentage of carbon dioxide is ratio of 0:100 (CO2=4.1 %) and the lowest is ratio 100:0 (CO2=0.9 %). Percentage of methane to compare with total percentage of methane and carbon dioxide has the highest percentage is the ratio of 25:75 (CH4=63.9 %), and the lowest percentage is the ratio of 100:0 (CH4=35.7 %). The highest to compare the lowest, percentage of methane per total percentage of methane and carbon dioxide is percentage of methane ratio 25:75 more than ratio 100:0 is 1.79.

scholarly journals The ManureEcoMine pilot installation: advanced integration of technologies for the management of organics and nutrients in livestock waste

2016 ◽  
Vol 75 (6) ◽  
pp. 1281-1293 ◽  
Author(s):  
Cristina Pintucci ◽  
Marta Carballa ◽  
Sam Varga ◽  
Jimena Sarli ◽  
Lai Peng ◽  
...  
Keyword(s):  
Production Rate ◽  
Biogas Production ◽  
Liquid Fraction ◽  
Swine Manure ◽  
Integrated Approach ◽  
Gas Production ◽  
Phosphorus Recovery ◽  
Organic Loading Rate ◽  
Livestock Waste ◽  
Nitrogen And Phosphorus

Manure represents an exquisite mining opportunity for nutrient recovery (nitrogen and phosphorus), and for their reuse as renewable fertilisers. The ManureEcoMine proposes an integrated approach of technologies, operated in a pilot-scale installation treating swine manure (83.7%) and Ecofrit® (16.3%), a mix of vegetable residues. Thermophilic anaerobic digestion was performed for 150 days, the final organic loading rate was 4.6 kgCOD m−3 d−1, with a biogas production rate of 1.4 Nm3 m−3 d−1. The digester was coupled to an ammonia side-stream stripping column and a scrubbing unit for free ammonia inhibition reduction in the digester, and nitrogen recovery as ammonium sulphate. The stripped digestate was recirculated daily in the digester for 15 days (68% of the digester volume), increasing the gas production rate by 27%. Following a decanter centrifuge, the digestate liquid fraction was treated with an ultrafiltration membrane. The filtrate was fed into a struvite reactor, with a phosphorus recovery efficiency of 83% (as orthophosphate). Acidification of digestate could increment the soluble orthophosphate concentration up to four times, enhancing phosphorus enrichment in the liquid fraction and its recovery via struvite. A synergistic combination of manure processing steps was demonstrated to be technologically feasible to upgrade livestock waste into refined, concentrated fertilisers.

scholarly journals Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0254730
Author(s):  
Fan Yang ◽  
Daniel S. Andersen ◽  
Steven Trabue ◽  
Angela D. Kent ◽  
Laura M. Pepple ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Methane Production ◽  
Swine Manure ◽  
Strongly Correlated ◽  
Strong Correlations ◽  
Community Assemblage ◽  
Hydrogenotrophic Methanogenesis ◽  
Deep Pit ◽  
Methane Production Rate ◽  
Deep Pits

Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and stabilization of methane. Here, we conducted a 13-month field study to survey the physical, chemical, and biological changes of pit-manure across 46 farms in Iowa. Our results showed that an increased methane production rate was associated with less digestible feed ingredients, suggesting that diet influences the storage pit’s microbiome. Targeted sequencing of the bacterial 16S rRNA and archaeal mcrA genes was used to identify microbial communities’ role and influence. We found that microbial communities in foaming and non-foaming manure were significantly different, and that the bacterial communities of foaming manure were more stable than those of non-foaming manure. Foaming manure methanogen communities were enriched with uncharacterized methanogens whose presence strongly correlated with high methane production rates. We also observed strong correlations between feed ration, manure characteristics, and the relative abundance of specific taxa, suggesting that manure foaming is linked to microbial community assemblage driven by efficient free long-chain fatty acid degradation by hydrogenotrophic methanogenesis.

scholarly journals Effects of Lighter Dose of Oxytetracycline on the Accumulation and Degradation of Volatile Fatty Acids in the Process of Thermophilic Anaerobic Digestion of Swine Manure

2021 ◽  
Vol 13 (7) ◽  
pp. 4014
Author(s):  
Zijing Fan ◽  
Mei Zhang ◽  
Xiaxia Chen ◽  
Zhongda Hu ◽  
Qihang Shu ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Respiratory Infections ◽  
Swine Manure ◽  
Inhibitory Effect ◽  
Farm Animals ◽  
Original Form ◽  
Livestock Farming ◽  
Thermophilic Anaerobic Digestion

Oxytetracycline (OTC) is a commonly used antibiotic in livestock farming for controlling intestinal and respiratory infections in farm animals. However, the absorption of antibiotics by animals is limited, and most antibiotics are excreted in the original form with manure, which will have an impact on the environment. The removal of antibiotics from swine manure could generally be performed via anaerobic digestion (AD). In this study, the effect of oxytetracycline (OTC) at doses of 0.1, 0.5, and 1.0 mg/L on the thermophilic anaerobic digestion of swine manure (55 °C) in batch digesters was studied. The methane production, volatile fatty acid (VFA) levels, and dissolved organic matter (DOM) were determined and compared with the control (0 mg/L of OTC). The results indicate that (1) OTC at 0.1 mg/L had no inhibitory effect on methane production or on the accumulation of VFAs, while 0.5 mg/L and 1.0 mg/L inhibited methane production, with inhibition rates of 4.03% and 14.12% (p < 0.05), respectively; (2) the VFAs of each reactor peaked on the first day of the reaction, and as the OTC dose increased from 0 to 1.0 mg/L, the maximum VFA accumulation increased from 1346.94 mg/g to 2370 mg/g of volatile solids (VS); and (3) oxytetracycline (0.5 and 1.0 mg/L) could promote the temporary accumulation of propionic acid, which did, however, not result in significant VFA accumulation. Further, OTC at 1.0 mg/L can promote DOM production, and therefore, VFA accumulation.

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