Phosphorus excretion from ruminants implanted with estrogenic growth implants

2013 ◽  
Author(s):  
D. R. Niemann ◽  
D. R. George ◽  
L. W. Greene ◽  
N. A. Cole ◽  
F. T. McCollum and N. K. Chirase
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jiqing Zhang ◽  
Sanjay Kumar ◽  
Muthuvel Jayachandran ◽  
Loren P. Herrera Hernandez ◽  
Stanley Wang ◽  
...  

Abstract Backgrounds: Previous studies have demonstrated that excretion of urinary extracellular vesicles (EVs) from different nephron segments differs between kidney stone formers and non-stone formers (NSFs), and could reflect pathogenic mechanisms of urinary stone disease. In this study we quantified selected populations of specific urinary EVs carrying protein markers of immune cells and calcium/phosphorus physiology in calcium oxalate stone formers (CSFs) compared to non-stone formers (NSFs). Methods Biobanked urine samples from CSFs (n = 24) undergoing stone removal surgery and age- and sex- matched NSFs (n = 21) were studied. Urinary EVs carrying proteins related to renal calcium/phosphorus physiology (phosphorus transporters (PiT1 and PiT2), Klotho, and fibroblast growth factor 23 (FGF23); markers associated with EV generation (anoctamin-4 (ANO4) and Huntington interacting protein 1 (HIP1)), and markers shed from activated immune cells were quantified by standardized and published method of digital flow cytometry. Results Urine excretion of calcium, oxalate, phosphorus, and calcium oxalate supersaturation (SS) were significantly higher in CSFs compared to NSFs (P < 0.05). Urinary excretion of EVs with markers of total leukocytes (CD45), neutrophils (CD15), macrophages (CD68), Klotho, FGF23, PiT1, PiT2, and ANO4 were each markedly lower in CSFs than NSFs (P < 0.05) whereas excretion of those with markers of monocytes (CD14), T-Lymphocytes (CD3), B-Lymphocytes (CD19), plasma cells (CD138 plus CD319 positive) were not different between the groups. Urinary excretion of EVs expressing PiT1 and PiT2 negatively (P < 0.05) correlated with urinary phosphorus excretion, whereas excretion of EVs expressing FGF23 negatively (P < 0.05) correlated with both urinary calcium and phosphorus excretion. Urinary EVs with markers of HIP1 and ANO4 correlated negatively (P < 0.05) with clinical stone events and basement membrane calcifications on papillary tip biopsies. Conclusions Urinary excretion of EVs derived from specific types of activated immune cells and EVs with proteins related to calcium/phosphorus regulation differed between CSFs and NSFs. Further validation of these and other populations of urinary EVs in larger cohort could identify biomarkers that elucidate novel pathogenic mechanisms of calcium stone formation in specific subsets of patients.


1936 ◽  
Vol 26 (1) ◽  
pp. 85-100 ◽  
Author(s):  
R. H. Common

1. Where heavy phosphorus excretion accompanies egg laying in the pullet the excretion of ammonia nitrogen is simultaneously increased. It is probable that this indicates an excretion of excess phosphate in the urine as ammonium phosphate.2. It is shown that heavy phosphorus excretion does not accompany egg laying provided the calcium carbonate intake is sufficiently high.3. The origin of the excess of phosphorus excretion is discussed in relation to calcium-phosphorus metabolism.4. Pullets on a ration containing 5 per cent, calcium carbonate laid eggs containing a higher percentage of P2O5 than pullets receiving a similar ration but from which the calcium carbonate supplement was omitted.5. Some evidence is put forward in support of the view that current standards pitch the requirements of digestible protein for egg production at too high a level.


Nature ◽  
1961 ◽  
Vol 189 (4766) ◽  
pp. 759-760 ◽  
Author(s):  
S. HURWITZ ◽  
P. GRIMINGER

2001 ◽  
Vol 89 (1-2) ◽  
pp. 113-118 ◽  
Author(s):  
K. Żyła ◽  
J. Koreleski ◽  
S. Świątkiewicz ◽  
D.R. Ledoux ◽  
J. Piironen
Keyword(s):  

2015 ◽  
Vol 29 (7) ◽  
pp. 960-964 ◽  
Author(s):  
B. Zhang ◽  
C. Wang ◽  
Z. H. Wei ◽  
H. Z. Sun ◽  
G. Z. Xu ◽  
...  

1997 ◽  
Vol 12 (5) ◽  
pp. 961-964 ◽  
Author(s):  
D. Rosenbaum ◽  
S. Holmes-Farley ◽  
W. Mandeville ◽  
M. Pitruzzello ◽  
D. Goldberg

2017 ◽  
Vol 95 (8) ◽  
pp. 3487-3496
Author(s):  
Q. B. Qu ◽  
P. Yang ◽  
R. Zhao ◽  
S. L. Zhi ◽  
Z. W. Zhai ◽  
...  

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