scholarly journals Cultivation of the brain-corpora cardiaca-corpora allata complex of the silkworm, Bombyx mori and eclosion hormone activity of the cultured organs and medium.

1990 ◽  
Vol 34 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Mika SAKAKIBARA ◽  
Chitose ORIKASA ◽  
Hajime FUGO
Keyword(s):  
Bombyx Mori ◽  
Corpora Allata ◽  
Hormone Activity ◽  
Corpora Cardiaca ◽  
Eclosion Hormone ◽  
The Brain ◽  
Silkworm Bombyx Mori

scholarly journals Peptidomic Analysis of the Brain and Corpora Cardiaca-Corpora Allata Complex in the Bombyx mori

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Xiaoguang Liu ◽  
Xia Ning ◽  
Yan Zhang ◽  
Wenfeng Chen ◽  
Zhangwu Zhao ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Developmental Stages ◽  
Larval Instar ◽  
Corpora Allata ◽  
Corpora Cardiaca ◽  
Rp Hplc ◽  
Transcriptome Database ◽  
Peptidomic Analysis ◽  
Peptide Profiles ◽  
The Brain

The silkworm, Bombyx mori, is an important economic insect for silk production. However, many of the mature peptides relevant to its various life stages remain unknown. Using RP-HPLC, MALDI-TOF MS, and previously identified peptides from B. mori and other insects in the transcriptome database, we created peptide profiles showing a total of 6 ion masses that could be assigned to peptides in eggs, including one previously unidentified peptide. A further 49 peptides were assigned to larval brains. 17 new mature peptides were identified in isolated masses. 39 peptides were found in pupal brains with 8 unidentified peptides. 48 were found in adult brains with 12 unidentified peptides. These new unidentified peptides showed highly significant matches in all MS analysis. These matches were then searched against the National Center for Biotechnology Information (NCBI) database to provide new annotations for these mature peptides. In total, 59 mature peptides in 19 categories were found in the brains of silkworms at the larval, pupal, and adult stages. These results demonstrate that peptidomic variation across different developmental stages can be dramatic. Moreover, the corpora cardiaca-corpora allata (CC-CA) complex was examined during the fifth larval instar. A total of 41 ion masses were assigned to peptides.

Physiology of Insect Ecdysis

1973 ◽  
Vol 58 (3) ◽  
pp. 821-829
Author(s):  
JAMES W. TRUMAN
Keyword(s):  
Nervous System ◽  
Manduca Sexta ◽  
Hormone Activity ◽  
Hormonal Factors ◽  
Corpora Cardiaca ◽  
Eclosion Hormone ◽  
Neural Input ◽  
The Brain ◽  
Pupal Cuticle

1. In pharate Manduca sexta moths eclosion hormone activity was present in the brain and corpora cardiaca. Bursicon activity was confined to the abdominal nervous system, and was most concentrated in the abdominal perivisceral organs (PVOs). 2. When newly emerged moths were given access to suitable wing-spreading sites, bursicon activity was depleted from the PVOs and appeared in the blood within 15 min after eclosion. This hormone was responsible for the tanning and hardening of the wings. 3. Bursicon release could be delayed for at least 24 h by forcing the newly emerged moth to dig. Secretion then occurred swiftly upon giving the moth a suitable wing-spreading site. 4. The pupal cuticle was removed from pharate Manduca approximately 7 h before their normal eclosion gate, and the peeled moths were provided with a wing-spreading site. These moths did not then secrete bursicon until after their normal time of eclosion. 5. Injection of the eclosion hormone into pharate moths caused early eclosion followed by precocious bursicon secretion. 6. It was concluded that bursicon release is regulated by both neural and hormonal factors. The eclosion hormone triggers a program of neural output which includes the secretion of bursicon. This release, however, can be delayed by neural input which is associated with the digging behaviour of the moth.

Eclosion hormone activity in developing embryos of the silkworm, Bombyx mori

1985 ◽  
Vol 31 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Hajime Fugo ◽  
Hitoshi Saito ◽  
Hiromichi Nagasawa ◽  
Akinori Suzuki
Keyword(s):  
Bombyx Mori ◽  
Hormone Activity ◽  
Eclosion Hormone ◽  
Silkworm Bombyx Mori
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