scholarly journals Cross Resistance and Synergism Studies in the Diamondback Moth, Plutella xylostella L.(Lepidoptera : Yponomeutidae)

1987 ◽  
Vol 22 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Virapong NOPPUN ◽  
Tadashi MIYATA ◽  
Tetsuo SAITO
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Cross Resistance

Evaluation of resistance, cross-resistance and synergism of abamectin and teflubenzuron in a multi-resistant field population of Plutella xylostella (Lepidoptera: Plutellidae)

1997 ◽  
Vol 87 (5) ◽  
pp. 481-486 ◽  
Author(s):  
M. Iqbal ◽  
D.J. Wright
Keyword(s):  
Plutella Xylostella ◽  
Maleic Acid ◽  
Diamondback Moth ◽  
Laboratory Strain ◽  
Piperonyl Butoxide ◽  
Diethyl Ester ◽  
Cross Resistance ◽  
Glutathione S Transferases ◽  
Pre Treatment ◽  
Selection Of

AbstractThe efficacy of abamectin (AgrimecR) and teflubenzuron (NomoltR) was assessed by leaf-dip bioassay against larvae of the diamondback moth, Plutella xylostella Linnaeus from a population (SERD3) collected originally in lowland Malaysia in December 1994. Evidence for resistance to both abamectin and teflubenzuron was found in the F7 generation (LC50 ratio of 60 and 24 respectively compared with a laboratory, insecticide-susceptible strain). Selection of sub-populations of SERD3 (F7–F9) with abamectin and teflubenzuron increased the LC50 ratio to 220 and 360 respectively and estimates of realized heritability [h2] were high (c. 0.8 and 0.9) for both compounds. There was no cross-resistance between these compounds in the abamectin and teflubenzuron-selected sub-populations but some indication of negatively-correlated resistance. Topical application of the synergists piperonyl butoxide, S,S,S-tributylphosphorotrithioate and maleic acid diethyl ester to the laboratory strain had no significant effect on the toxicity of abamectin or teflubenzuron in subsequent leaf-dip assays. In contrast, pre-treatment with piperonyl butoxide and S,S,S-tributylphosphorotrithioate significantly increased the toxicity of abamectin (c. 4- and 3-fold) and teflubenzuron (c. 7- and 19-fold) in the abamectin and teflubenzuron-selected sub-populations of SERD3, suggesting that microsomal monoxygenases and/or esterases may be involved in resistance. Pre-treatment with maleic acid diethyl ester only increased the toxicity of abamectin by c. 2-fold and had no significant effect on the toxicity of teflubenzuron, providing limited evidence for the involvement of glutathione-S-transferases in resistance to the former compound alone.

Inheritance and Cross Resistance of Phenthoate-Selected Diamondback Moth, Plutella xylostella (Lepidoptera: Yponomeutidae)

2004 ◽  
Vol 7 (2) ◽  
pp. 233-237 ◽  
Author(s):  
No-Joong Park ◽  
Sae-Chan Oh ◽  
Yong-Ho Choi ◽  
Kwang-Ryeul Choi ◽  
Kwang Yun Cho
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Cross Resistance

scholarly journals Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in Plutella xylostella

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 311
Author(s):  
Jun Shen ◽  
Zhao Li ◽  
Dongyang Li ◽  
Rumeng Wang ◽  
Shuzhen Zhang ◽  
...  
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Management Strategies ◽  
Detoxification Enzymes ◽  
Cross Resistance ◽  
Transferase Activity ◽  
Glutathione S Transferase ◽  
Obvious Effect ◽  
Stability Of Resistance ◽  
Biochemical Mechanisms

The diamondback moth, Plutella xylostella (L.) is an important pest of cruciferous crops worldwide. It has developed resistance to many conventional and novel insecticide classes. Metaflumizone belongs to the new chemical class of semicarbazone insecticides. To delay the development of metaflumizone resistance in P. xylostella and to guide insecticide use in the field, the biochemical mechanisms, cross-resistance spectrum, and stability of resistance to metaflumizone were studied in a laboratory-selected resistant strain (metaflu-SEL). Synergism tests with the carboxylesterase inhibitor triphenyl phosphate (TPP), the glutathione S-transferase depletor diethyl maleate (DEM), and the P450 inhibitor piperonyl butoxide(PBO) had no obvious effect on metaflumizone in the metaflu-SEL strain and the susceptible strain (SS) of P. xylostella, with synergism ratios that ranged from 1.02 to 1.86. Biochemical studies revealed that the cytochrome P450-dependent monooxygenase increased only 1.13-fold in the metaflu-SEL strain compared with the UNSEL stain; meanwhile, carboxylesterase and glutathione S-transferase activity showed no difference. These results suggest that these detoxification enzymes may be not actively involved in metaflumizone resistance. Furthermore, the metaflu-SEL population showed a moderate level of cross-resistance to indoxacarb (11.63-fold), but only very low cross-resistance to spinosad (1.75-fold), spinetoram (3.52-fold), abamectin (2.81-fold), beta-cypermethrin (0.71-fold), diafenthiuron (0.79-fold), chlorantraniliprole (2.16-fold), BT (WG-001) (3.34-fold), chlorfenapyr (0.49-fold), and chlorfluazuron (0.97-fold). Moreover, metaflumizone resistance decreased from 1087.85- to 1.23-fold in the metaflu-SEL strain after 12 generations without exposure to metaflumizone. These results are useful for formulating insecticide resistance management strategies to control P. xylostella and to delay the development of metaflumizone resistance in the field.

Sign in / Sign up

Export Citation Format

Share Document