scholarly journals Antioxidant Activity of Multifarious Solvent Extracts of Leaf. (Plumbago Zeylanica)

2019 ◽  
Vol 35 (2) ◽  
pp. 718-722
Author(s):  
D. Roselin Jenifer ◽  
B. R. Malathy
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Assay Method ◽  
Dpph Assay ◽  
Leaf Extracts ◽  
Plumbago Zeylanica ◽  
Reducing Power Assay

The current investigation is focused to determine antioxidant activities of the leaf extract of Plumbago zeylanica. The leaf extracts were prepared by using multiform solvents with increasing polarity, including as hexane, chloroform, ethyl acetate and Methanol. The antioxidant activities enlisting DPPH assay, hydrogen peroxide assay, reducing power assay and nitric oxide assay method were performed for all extracts. Barring no one, the extracts had shown average to potent antioxidant activity. Uniquely, the ethyl acetate extract and the chloroform extract explicated the utmost antioxidant activity.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (10) ◽  
pp. 34-41
Author(s):  
Kanthlal S. K. ◽  
Jipnomon Joseph ◽  
Bindhu P. Paul ◽  
Vijayakumar M ◽  
Rema Shree A. B. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Bioactive Constituents ◽  
Preliminary Assessment ◽  
Reducing Power Assay ◽  
Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

scholarly journals Analgesic and Antioxidant Activities of 4-Phenyl-1,5-benzodiazepin-2-one and Its Long Carbon Chains Derivatives

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Terence Nguema Ongone ◽  
Redouane Achour ◽  
Mostafa El Ghoul ◽  
Latyfa El Ouasif ◽  
Meryem El Jemli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Carbon Chain ◽  
Immersion Test ◽  
Tail Immersion ◽  
Reducing Power Assay

The aim of this work is to deepen the pharmacological effect of 4-phenyl-1,5-benzodiazepin-2-one derivatives which have a similar structure to nonionic surfactants: 4-phenyl-1,5-benzodiazepin-2-one is the hydrophilic head, and the carbon chain is hydrophobic tail. The antinociceptive activity of 4-phenyl-1,5-benzodiazepin-2-one derivatives was determined using acetic acid-induced writhing and tail immersion tests. In addition, the in vitro antioxidant activities of the tested derivatives were determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferric reducing power assay. A single oral administration of these compounds at the doses of 50 and 100 mg/kg significantly reduced the number of abdominal writhes induced by acetic acid injection. Acute pretreatment with 4-phenyl-1,5-benzodiazepin-2-one derivatives at the dose of 100 mg/kg caused a significant increase in the tail withdrawal latency in the tail immersion test. Additionally, a significant scavenging activity in DPPH and reducing power was observed in testing antioxidant assays. Finally, we carried out a study of the antioxidant activity of these derivatives. The results of this study reveal that these compounds have a low antioxidant activity compared to the BHT. It decreases with the polarity of the molecule. The present study suggests that 4-phenyl-1,5-benzodiazepin-2-one derivatives possess potent antinociceptive and antioxidant effects, which suggest that the tested compounds may be useful in the treatment of pain and oxidation disorders.

scholarly journals Pharmacognostical Standardisation of Ailanthus Excelsa Leaves and Randia Dumetorum Fruit Along with Antioxidant Activity and Free Radical Scavenging Capacity of Its Fractions

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1213-1221
Author(s):  
Vivek V Paithankar ◽  
Shailesh M Kewatkar ◽  
Trupti A Nimburkar ◽  
Supriya S Deshpande
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Herbal Product ◽  
Assay Method ◽  
Phytochemical Analysis ◽  
Ailanthus Excelsa ◽  
The World ◽  
Saponin Fraction ◽  
Reducing Power Assay

The world is observing an unprecedented development in the usage of herbal product at national as well as international levels. This requires the improvement of current and aimed standards for estimating the quality, safety and efficacy of these drugs. The leaves of Ailanthus excelsa and the fruits of Randia Dumetorum are medicinal plants that are used for many diseases around the world. We then collected the flavonoids and saponin fraction extracted from the leaves of Ailanthus excelsa and the fruits of Randia dumetorum. To determine the reliability, quality and purity of these particles, we provide a crucial pharmacological profile along with the antioxidant activity. Pharmacological studies, such as morphological, physicochemical, TLC, and phytochemical analysis of all fractions containing total phenol and flavonoids, were performed according to specific methods. DPPH tests estimated the antioxidant action of all fractions, Hydrogen peroxide scavenging assay, and reducing power assay method. Previous phytochemical studies discovered the occurrence of saponins, flavonoids, tannins, and especially phenolic chemicals. All fractions have antioxidant effects, depending on the existence of a phenolic compound. The above parameters are vital to establishing pharmacological rules for the authentication of Ailanthus excelsa leaves and Randia Dumetorum fruits.

scholarly journals Pharmacological Evaluation of Antipyretic and Antioxidant Activities of 80% Methanol Root Extract and Derived Solvent Fraction of Echinops kebericho M. (Asteraceae) in Mice Model

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Tesfaye Yimer ◽  
Yohannes Kelifa Emiru ◽  
Zemene Demelash Kifle ◽  
Amien Ewunetei ◽  
Meaza Adugna ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Assay Method ◽  
Root Extract ◽  
Standard Drug ◽  
Aqueous Fraction ◽  
Temperature Reduction ◽  
Solvent Fraction

Background. Toxicity and untoward effects are very ostensible in most standard drugs including antipyretic agents. Searching for conceivable antipyretic drugs with minimal toxicities and side effects from traditional plants is a growing concern to date. Echinops kebericho M. (Asteraceae) is one of the most prominent traditional medicinal plants, which is frequently testified for its traditionally claimed uses of treating fever and different infectious and noninfectious disorders by traditional healers in Ethiopian folk medicine. However, this plant has not been scientifically assessed for its traditionally claimed uses. This study therefore is aimed at investigating the antipyretic and antioxidant activities of 80% methanol root extract and the derived solvent fraction of Echinops kebericho M. in mouse models. Methods. Successive solvent maceration with increased polarity was used as the method of extractions, and chloroform, ethyl acetate, methanol, and water were used as solvents. After extraction, the crude extract and its derived solvent fractions were assessed for their antipyretic activities using yeast-induced pyrexia while, the antioxidant activities were measured in vitro using the diphenyl-2-picrylhydrazyl (DPPH) assay method. Both the extract and solvent fractions were evaluated at the doses of 100, 200, and 400 mg/kg for its antipyretic activities, and the antioxidant activity was evaluated at the doses of 50, 100, 200, 400, 600, 800, and 1000 mg/kg. The positive control group was treated with standard drug (ASA 100 mg/kg), while normal saline-receiving groups were assigned as negative control. Result. E. kebericho crude extract along with its derived solvent fractions showed statistically significant ( p < 0.05 , 0.01, and 0.001) temperature reduction activities. The maximum percentage of temperature reduction was observed by the highest dose (400 mg/kg) of the crude extract. The aqueous fraction also showed significantly ( p < 0.05 and 0.01) higher temperature reduction than those of ethyl acetate and chloroform fractions. The free radical scavenging activities of the crude extract were also significantly high at the maximum dose, and the aqueous fraction showed the significantly highest antioxidant activity. Conclusion. In general, the data obtained from the present study clarified that the extract possessed significant antipyretic and antioxidant activities, upholding the traditionally claimed use of the plant.

ANTIOXIDANT ACTIVITY OF FOREST MANGGOSTEEN (Garcinia hombroniana Pierre) FRACTION USING DPPH AND ABTS METHOD

2021 ◽  
Vol 9 (Spl-2-ICOPMES_2020) ◽  
pp. S280-S285
Author(s):  
Ismail . ◽  
◽  
Mufidah . ◽  
Sukamto S. Mamada ◽  
Amrianto . ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Bark Extract ◽  
Active Fraction ◽  
Dpph Assay ◽  
Abts Assay ◽  
Ic50 Value ◽  
Vacuum Column ◽  
Qualitative Determination

This study was carried out to determine the active antioxidant fraction of Garcinia hombroniana bark extract by the DPPH and ABTS method. Along with this, the study also attempts to identify the class of compounds present in the various extract of G. hombroniana by the active fraction. The bark extract of G. hombroniana was prepared by the multilevel maceration method by using three solvents including n-hexane, ethyl acetate, and 96% ethanol. Results of study suggested that n-hexane (HSE), ethyl acetate (EASE) and ethanol 96% extract (ESE) have antioxidant activity with IC50 value of 423 ± 16.72 μg/mL, 284.89 ± 2.7μg/mL, and 10.49 ± 0.19 μg/mL in DPPH assay, while these extracts showed IC50 value of 560.92 ± 48.86 μg/mL, 430.18 ± 16.65 μg/mL, and 13.92 ± 0.57 μg/mL respectively in ABTS assay. Further, fractionated using vacuum column chromatography revealed the presence of five fractions viz., A, B, C, D, and E. Among these, fractions D showed the highest antioxidant activity with an IC50 value of 4.83 ± 0.18 μg/mL and 6.82 ± 0.31 μg/mL in DPPH and ABTS assays. Results of the fractioned analysis and qualitative determination showed that the active fraction of G. hombroniana contained flavonoid, triterpenoid, alkaloid, and saponin compounds, and antioxidant activities of these extracts might be due to the presence of these active ingredients.

scholarly journals Dr. PI POLYPHENOL CONTENT, PHYTOCHEMISTRY COMPOSITIONS AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS FROM MARINE SPONGE AAPTOS SUBERITOIDES GROWN IN NHATRANG BAY, VIETNAM

2019 ◽  
pp. 80-86
Author(s):  
DANG XUAN CUONG ◽  
VU NGOC BOI ◽  
TRAN KHAC TRI NHAN ◽  
DINH HUU DONG ◽  
THAI MINH QUANG ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Marine Sponge ◽  
Functional Food ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Polyphenol Content ◽  
Significant Difference ◽  
Dpph Scavenging

Objective: To investigate the content and antioxidant activities of polyphenol, the correlation between polyphenol content and their antioxidant activities, and phytochemistry compositions of different extracts from marine sponge Aaptos suberitoides commonly found growing in Nhatrang bay, Vietnam orienting application into functional food and pharmacy. Methods: Evaluating the toxicity of antioxidant polyphenol powder preparing from the initial concentrated extract was by the adjusted Behrens Karber method and a correlation between polyphenol content and antioxidant activities basing on the Pearson coefficient in Excel. Separating antioxidant polyphenol content was base on solvents polarization of n-hexane, chloroform, ethanol, ethyl acetate and n-butanol which the quantification of polyphenol content and antioxidant activities, and preliminary phytochemical compositions qualitative. Results: Antioxidant polyphenol powder did not affect mice weight during the assay time of 28 d. Polyphenol content and antioxidant activities got the highest value at chloroform extract in comparison to other extracts, a significant difference (p<0.05) and strong correlation (R2>0.9). Polyphenol content (122.682 mg gallic acid equivalent ml-1), total antioxidant activity (368.183 mg ascorbic acid equivalent ml-1), reducing power activity (24.08 mg FeSO4 equivalent ml-1) and DPPH scavenging (72.48±1.54 %) were the highest values. Alkaloids, flavonoids, steroids, tannins and triterpenoids existed in initial methanol extract. Weakly polarized polyphenol content was 70.27% in comparison to initial methanol extract. Conclusion: Antioxidant polyphenol of sponge Aaptos suberitoides has the potential for application into the field of functional food and pharmaceuticals.

scholarly journals Antioxidant activity of leaves and bark extracts of Crataeva magna plant

2021 ◽  
Vol 5 (1) ◽  
pp. 001-008
Author(s):  
Faheem I P ◽  
B. Gopalakrishna ◽  
Mohsina FP ◽  
Sarah Priya
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Reducing Power Assay ◽  
Dose Dependent

Recently, a substantial body of literature provides strong evidence to support the potentials of Crataeva magna (CM), plant and its extracts in various pathological conditions. Thus, the CM plant extract at various concentrations were prepared and antioxidant activity was evaluated. The fresh leaves and bark of CM were collected and various extracts such as n-Hexane, Chloroform, Ethanol and aqueous extract were prepared. The preliminary phytochemical investigation showed the presence of various phytochemical such as total phenolic, flavonoid compounds and tannins. The ethanolic leaves extract of CM (ELECM) and water leaves extract of CM (WLECM), showed dose dependent increased in free radical scavenging activity in DPPH assay and reducing power assay in present study. Moreover, ethanolic barks extract of CM (EBECM) and water barks extract of CM (WBECM), also showed increased in free radical scavenging activity in DPPH assay and reducing power assay. However, the effects of bark extracts were lower than leaves extracts. In the light of the above consideration, the results of the study revealed that ELECM showed promising antioxidant activity in a dose dependent manner.

scholarly journals In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts

2020 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mounira Merghem ◽  
Saliha Dahamna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Ruta Montana ◽  
Β Carotene

The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L.  extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract,  251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene. Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.

Sign in / Sign up

Export Citation Format

Share Document