scholarly journals A Probe into Prohealing Potential of Glyceryl Trinitrate

2019 ◽  
Vol 12 (04) ◽  
pp. 1761-1765
Author(s):  
Smita A. Surale-Patil ◽  
Tatyasaheb Patil ◽  
Ravindra Kulkarni
Keyword(s):  
Wound Healing ◽  
Glyceryl Trinitrate ◽  
Wistar Rats ◽  
Wound Contraction ◽  
Spasmolytic Activity ◽  
Ointment Base ◽  
Spasmolytic Action ◽  
Healing Wound ◽  
Male Wistar Rats ◽  
Two Parameters

Wound healing is a complex, delicate process which consists of series of cellular and biochemical events leading to re-establishment of anatomical continuity. Anal fissure is a painful, chronic, non-healing wound. Its chronicity is due to spasm of anal sphincter and local ischemia. It is treated surgically by sphincterotomy to eliminate sphincter spasm. Therefore, Glyceryl trinitrate(GTN) has been used as a non-surgical alternative treatment due to its spasmolytic activity. The present study was undertaken to determine wound healing potential of GTN in wistar rats. 18 male wistar rats were divided in three groups, 6 rats per group Control (Normal saline), GTN, GTN Ointment base. A 500 mm2 excisional wound , circular in shape was created . The drugs were applied topically over the wound, one day after the wounding. Two parameters were studied viz; wound contraction and period of epithelization. Glyceryl trinitrate has shown statistically signfiicant difference in wound contraction compared to control on 10th day. Period of epithelization was not reduced significantly by Glyceryl trinitrate when compared to Control. Glyceryl trinitrate has wound healing potential apart from its spasmolytic action.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals Burn Wound Healing Activity of Prunella Grandiflora L. Exctract

Biomeditsina ◽  
2021 ◽  
Vol 17 (4) ◽  
pp. 50-56
Author(s):  
E. V. Bolotnik ◽  
L. I. Alekseeva ◽  
L. P. Larionov
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Dosage Forms ◽  
Control Groups ◽  
Burn Wound ◽  
Dry Extract ◽  
Burn Wound Healing ◽  
Male Wistar Rats ◽  
And Control ◽  
Wound Healing Activity

The qualitative composition of phenolic acids and burn wound healing activity of Prunella grandiflora L. extract were studied. The content of rosmarinic (621±5 mg/g), coffeic (11.2±0.2 mg/g), syringic (46.5±1.4 mg/g), n-coumaric (25.8±0.5 mg/g), and ferulic (42.7±2.9 mg/g) acids in dry extracts of P. grandiflora was determined by HPLC. Three new burn-healing agents of different composition containing 5% of the dry extract of Prunella grandiflora L. (big-flowered self-heal) were developed. The developed agents were tested on male Wistar rats with second- and third-degree thermal burns. An assessment of skin restoration was carried out in experimental and control groups of animals. It was revealed that animals treated with the developed soft dosage forms restored the epithelium and coat 6–8 days faster that those treated with compositions containing no extract under study. It was found that the developed soft dosage forms based on P. grandiflora extract exhibit anti-inflammatory and wound-healing action.

scholarly journals Determination of wound healing activity of Shark liver oil using the excision wound model in Wistar rats

2020 ◽  
Vol 11 (4) ◽  
pp. 7301-7308
Author(s):  
Auti S A ◽  
Jat R K ◽  
Das S K ◽  
Shukla S T
Keyword(s):  
Wound Healing ◽  
Disease Control ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Wound Contraction ◽  
Standard Drug ◽  
Wound Area ◽  
Excision Wound ◽  
Shark Liver Oil ◽  
Wound Model

There are various promising preclinical models, such as in mice, wistar rats, rabbits and pigs, which can be utilized to initiate acute or chronic wounds. These can be persuaded by many distinctive techniques, with excision the most common. After determining a proper model for a study, investigators need to choose an appropriate and reproducible technique that will permit the monitoring of the wound improvement over time. In this study, the healing power of Shark Liver Oil Emulgel (SLO) in Wistar rats were analyzed by using the excision wound model. The shark liver oil was prepared as emulgel at a concentration of 5%,10% and 15%, respectively and both Standard drug(Povidone-Iodine Ointment USP 5% w/w-PI) and SLO is applied at a concentration of 1mg/mm2; Topically. The parameters integrated for the assessment of the effects of SLO were Relative body weight changes, wound area contraction in mm2, relative wound percentage and epithelialization time. Wound area contraction was measured on 1st, 3rd, 6th, 9th, 12th, 15th, 18th and 21st day. Wistar rats treated with SLO showed substantial variations in epithelialization period and improved wound contraction in the excision wound treatment as compared to disease control. The biochemical biomarkers like SOD(Superoxide dismutases), GSH(Reduced Glutathione)and LPO(Lipid peroxidase) in the treated group have shown a significant change in the improvement of wound healing. Histopathological studies and microscopic observations specify that the topical use of Shark liver oil Emulgel extensively improved wound contraction, collagenation and epithelialization with well-organized dermis devoid of inflammatory cells in contrast to disease control.

scholarly journals Preparation and evaluation of burns wound healing ointment base of leaves and stem bark of Anthocleista djalonensis (L) extract using animal model.

2020 ◽  
Vol 1 (02) ◽  
pp. 25-32
Author(s):  
Chinenye Nnenna Ugwu ◽  
Ezinwanne Nneoma Ezeibe ◽  
Chinekwu Sherridan Nwobi ◽  
Chinelo Charity Eze ◽  
Somtochukwu Amuche Evurani ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Bark ◽  
Positive Control ◽  
Silver Sulfadiazine ◽  
Wound Contraction ◽  
Bark Extract ◽  
Control Group ◽  
Burn Wound ◽  
Burn Wounds ◽  
Ointment Base

BACKGROUND: Burn wounds are inescapable life events especially in low income areas. Contamination of the burn wound site results in localized wound infection, hence the need for potent phytochemicals readily available for wound healing. The use of stem bark of Anthocleista djalonensis efficacy for burn wound has not been evaluated to the best of our knowledge. AIM: The study is aimed at evaluating and comparing the wound healing potential of ointment base of leaves and Stem bark of Anthocleista djalonensis on burn wounds created on wistar albino rats. METHODS: The leaves and Stem bark of Anthocleista djalonensis collected were extracted using 95%v/v methanol and phytochemical analysis conducted. Simple ointments of varying concentrations were formulated to screen for wound healing activity using the burn wound model on experimental rats grouped into six (n=4). Group 1 was treated with silver sulfadiazine cream (positive control), group 2 with ointment base (negative control), group 3 with 1% stem bark extract ointment, group 4 with 2% stem bark ointment, group 5 with 1% leave extract ointment, and group 6 with 2% leave extract ointment.  All animals were anesthetized before the creation of burn wounds. Measurement was taken on day zero and the wound was left untreated for 48 hours in order to allow bacterial colonization before daily treatment of the wound for 16 days. RESULTS: The result of the phytochemical screening revealed that both extract of Anthocleista djalonensis contains flavonoids, tannins and saponins. On day 2, 1% stem bark, 2% stem bark and 1% leaf extract had 16%, 15%, and 10% wound contraction respectively which was higher than the 8.5% wound contraction of silver sulfadiazine. Also as the concentration of the extract increased, the wound healing effect also increased as seen by the percentage wound contraction on day 16 for all treatment.  CONCLUSION: The findings of the study have shown that methanolic extracts of stem bark and leaf of Anthocleista djalonensis contained bioactive constituents which have burn wound healing activity. The stem bark extract showed better activity when compared with the leaf extract and also the positive control (silver sulfadiazine).    

scholarly journals Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardiumoccidentale Leaves in Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 2061-2068
Author(s):  
Meena Kumari K ◽  
Amberkar Mohanbabu Vittalrao ◽  
Praveen Kumar SE ◽  
Sushma Prabhath ◽  
Charitha Charitha
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Saline Control ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Healing Activity

BACKGROUND -This study undertaken to screen a potential wound healing activity of an ethanolic extract of Anacardiumoccidentale(Ao)leaves in Wistar rats. MATERIALS AND METHODS-Excision wound of 500mm2created on the back of Wistar rats. A total of thirty adult Wistar rats were prescreened and divided into five groups of six rats each.Group I and II rats were treated by normal saline (control) and povidone-iodine (standard drug), respectively. Group III and IV with 0.5% and 2%of Anacardiumoccidentale ointment (test drugs), respectively, and Group V rats treated with anti-wound healer drug dexamethasone, i.m. + 2% Anacardiumoccidentale. All drugs were applied topically except Inj. dexamethasone. In the model of excision wound, the wound healing effect was determined by parameters such as the percentage of wound contraction, duration of epithelialization, and histopathological examination. RESULT-All drugs, povidone-iodine, 0.5%, and 2% of the Ao ointment treated group of rats showed a statistically significant increase (P<0.05) in the % of wound contraction on 5th and 6thdays Vs.Control rats. In contrast,povidone-iodine, 2% Ao ointment and (dexamethasone + 2% Ao)treated rats decreased the period of epithelialization which was statistically significant (P<0.05) compared to the control.However, the standard povidone-iodine treated rats showed a maximum increase in the percentage of wound contraction and minimum period of epithelialization, followed by a higher dose of 2% Ao extract. The plant extract 0.5% and 2% of Ao showeda significant increase inthe rate of wound contraction as compared to the standard drug povidone-iodine also. The combination of (2%Aoextract+dexamethasone) drug-treated rats decreased the rate of wound contraction on 5th, 9th days; whereas, povidone-iodine increased on all days, i.e., 5th, 9th, 13th and 17th days,which was statistically significant (p<0.05) Vs.Normal saline. The povidone and 2% of Ao extract hastened the rate of wound healing activity, which was evident by maximum re-epithelialization and presence of the collagen fibers. CONCLUSION-In our study, the topical application of Anacardiumoccidentale leaf extract has shown promising wound healing activity; however, it failed to reverse the anti-wound healing property of steroids.

scholarly journals Effect of static magnetic field on experimental dermal wound strength

2012 ◽  
Vol 45 (02) ◽  
pp. 215-219 ◽  
Author(s):  
Yahya Ekici ◽  
Cem Aydogan ◽  
Cenk Balcik ◽  
Nihan Haberal ◽  
Mahir Kirnap ◽  
...  
Keyword(s):  
Wound Healing ◽  
Magnetic Fields ◽  
Mechanical Strength ◽  
Wistar Rats ◽  
The Other ◽  
Cutaneous Wound Healing ◽  
Static Magnetic Fields ◽  
Cutaneous Wound ◽  
Male Wistar Rats ◽  
Dermal Wound

ABSTRACT Context: An animal model. Aim: We sought to evaluate the effect of static magnetic fields on cutaneous wound healing. Materials and Methods: Male Wistar rats were used. Wounds were created on the backs of all rats. Forty of these animals (M group) had NeFeB magnets placed in contact with the incisions, either parallel (Pa) and perpendicular (Pr) to the incision. The other 40 animals (sham [S] group) had nonmagnetized NeFeB bars placed in the same directions as the implanted animals. Half of the animals in each group were killed and assessed for healing on postoperative day 7 and the other half on postoperative day 14. The following assessments were done: gross healing, mechanical strength, and histopathology. Statistical Analysis Used: Intergroup differences were compared by using the Mann-Whitney U or t test. Values for P less than 0.05 were accepted as significant. Results and Conclusions: There were no differences between the magnetic and sham animals with respect to gross healing parameters. The mechanical strength was different between groups. On postoperative day 14, the MPr14 had significantly higher scores than the other groups. When static, high-power, magnetic fields are placed perpendicular to the wound, increased wound healing occurs in the skin of the experimental model.

scholarly journals Unripe Rind and Pulp of Ananas comosus Accelerate Wound Healing in Diabetic Wistar Rat

2019 ◽  
pp. 1-13
Author(s):  
Rotimi Sunday Ajani ◽  
Nurudeen Abiola Opadokun
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Foot Ulcer ◽  
Wistar Rat ◽  
Wound Contraction ◽  
Ananas Comosus ◽  
Control Group ◽  
Accelerate Wound Healing ◽  
The Mean ◽  
Diabetic Wounds

Objective: One of the sequelae of diabetes mellitus is chronic foot ulcer. This study investigated wound healing propensity of the pulp and rind of Ananas comosus (a widely cultivated fruit with many health benefits) in diabetic wistar rats. Methods: The excisional wounds of twenty four adult wistar rats with induced diabetes in four groups of six each were dressed with the pulp powder (PPD), pulp extract (PED), rind powder (RPD) and rind extract (RED) of A. comosus daily till healed. Also the mean wound contraction rates were calculated from the wound areas every three days. Granulation tissue was biopsied from an animal per group on day 3, 6 and 9 for histopathological evaluation. Each of these groups had a corresponding equal number control group with similar procedures performed. Results: The mean wound contraction rates of the rind pulp diabetic (RPD) and rind extract diabetic (RED) groups paralleled those of their respective control group (RPC, REC) as they were not significantly different. Also amongst the diabetic groups, the rind of A. comosus demonstrated greater wound healing capability over the respective pulp counterpart (PPD, PED) as evidenced by significantly higher mean wound contraction rates. Histopathologic features of the granulation tissues and scars of the diabetic groups were similar to those of the control groups. Conclusion: Although, both the rind and pulp of unripe A. comosus either in extract or powder formulation were shown in this study to possess excellent healing potentials in diabetic wounds, the rind appeared to be better off and may be a viable alternative to the wound dressing materials currently in use in the management of diabetic wounds / ulcers.

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