scholarly journals Molecular Characterization of Thirteen Oil seed Brassica L. Variants From Bangladesh Through Polyacrylamide Gel Electrophoresis (PAGE)

2021 ◽  
Vol 17 (4) ◽  
pp. 741-755
Author(s):  
Faria Akbar ◽  
Susmita Saha ◽  
Meghla Saha Pinky ◽  
Kazi Nahida Begum
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Gel Electrophoresis ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Polyacrylamide Gel ◽  
Sufficient Information ◽  
Parental Line ◽  
Wide Range

Brassica L. is the most agronomical important genus of Brassicaceae family. An electrophoretic exploration was conveyed for proper identification of genetically diverse and agronomically superior genotypes and pursuing the extent of genetic divergence and phylogenetic relationship within the thirteen variants of Brassica for leaf storage protein by using Polyacrylamide Gel Electrophoresis (PAGE) as biochemical marker. A total of 19 alternative protein bands were found with high polymorphism of 89.47%. The protein banding pattern suggested the existence of differences among the studied variants pertaining to the location, molecular weight and staining intensity of the bands which could be utilized as fingerprints for variants identification. Based on Nei’s genetic distance, a wide range of genetic distance (0.0541–1.5581) offered the presence of broad genetic variability among the quested variants. A dendrogram was constructed by using UPGMA where all the analyzed Brassica variants were rouped into two major clusters. Relying on this analysis, highest genetic variation (1.5581) was observed between BS-10 and BS-14 while the lowest genetic variation (0.0541) was recorded between BS-9 and BS-12, which might be furnished as a source of parental line. Consequently, it can be proposed that the protein profile of the analyzed thirteen variants of Brassica L. by PAGE would be considered to be a contributory implement to the breeders of Brassica by providing sufficient information on the genetic resources of Brassica and improvement of new offspring in the forthcoming breeding program of Brassica L.

A biochemical and toxicological study of the role of insensitive acetylcholinesterase in organophosphorus resistantBemisia tabaci(Homoptera: Aleyrodidae) from Israel

1994 ◽  
Vol 84 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Frank J. Byrne ◽  
Matthew Cahill ◽  
Ian Denholm ◽  
Alan L. Devonshire
Keyword(s):  
Bemisia Tabaci ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Female Offspring ◽  
Polyacrylamide Gel ◽  
Microplate Assay ◽  
Toxicological Study ◽  
Single Insect

AbstractTwo acetylcholinesterase (AChE) variants, differing in sensitivity to inhibition by the organophosphorus (OP) insecticide paraoxon were identified in a population ofBemisia tabaci(Gennadius) from cotton in Israel using a single insect kinetic microplate assay. Two strains were established, homogeneous for one or other of the two variants, by isolating mated females from the field population onto individual cotton leaves, and testing a proportion of their female offspring to identify their AChE genotype. Polyacrylamide gel electrophoresis of their I-naphthyl butyrate hydrolyzing esterases showed that all insects contained esterase E0 14, which is indicative of B-type whiteflies, although the staining intensity of this band differed. Resistance to the OPs monocrotophos, profenofos and chlorpyrifos in leaf dip bioassays was consistent with the presence of the insensitive AChE. The data also indicated that separate mechanisms conferred resistance to the two pyrethroids cypermethrin and bifenthrin. The former, when used in a mixture with profenofos, was no more toxic than when the OP was used alone, and resistance to the mixture was largely dependent on the presence of the insensitive AChE.

Protein Profile of Human Perilymph: In Search of Markers for the Diagnosis of Perilymph Fistula and other Inner Ear Disease

1994 ◽  
Vol 111 (3P1) ◽  
pp. 273-280 ◽  
Author(s):  
Isolde Thalmann ◽  
Robert I. Kohut ◽  
Jay Ryu ◽  
Thomas H. Comegys ◽  
Masamitsu Senarita ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Inner Ear ◽  
Total Protein ◽  
Gel Electrophoresis ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Density Lipoprotein ◽  
Polyacrylamide Gel ◽  
Computer Assisted ◽  
Two Dimensional

Recent developments in high-resolution two-dimensional polyacrylamide gel electrophoresis, combined with amino acid sequencing and computer-assisted image analysis, have allowed separation of approximately 100 proteins and identification and quantitation of some 30 proteins in human perilymph. The majority of proteins were found to be present in perilymph at levels in basic agreement with the total protein gradient between perilymph and plasma (1:35). However, several striking differences were observed: (1) β2-transferrin, known to be absent from normal plasma but present in cerebrospinal fluid, was detected in perilymph at a concentration roughly equal to that in cerebrospinal fluid; and (2) two high-density lipoprotein-associated apolipoproteins—apo D (formerly PLS:33) and apo J or NA1 and NA2 (formerly PSL:29/30), the latter showing identity with 5P40/40, or cytolysis inhibitor—were found to be present at concentrations 1 to 2 orders of magnitude higher when examined in terms of total protein and to be comparable with or higher than plasma levels when examined in terms of absolute concentrations. The functional significance of the extremely high levels of the two apolipoproteins is not known at this time. An attempt was made to use β2-transferrin, as well as apo D and apo J (NA1/NA2), as markers for the diagnosis of perilymph fistula, one of the most controversial and challenging problems for the otologist today. It was determined that the technique is indeed applicable when relatively pure fistula samples are analyzed. Limitations and potential improvements of the technique are discussed. In addition, the potential usefulness of two-dimensional polyacrylamide gel electrophoresis in other pathologic conditions of the inner ear is discussed briefly.

Transferrin variation in caribou (Rangifer tarandus L.) on the Canadian Arctic islands

1986 ◽  
Vol 64 (1) ◽  
pp. 94-98 ◽  
Author(s):  
K. H. Røed ◽  
H. Staaland ◽  
E. Broughton ◽  
D. C. Thomas
Keyword(s):  
Genetic Distance ◽  
Gel Electrophoresis ◽  
Rangifer Tarandus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Canadian Arctic ◽  
High Arctic ◽  
Polyacrylamide Gel ◽  
Common Allele ◽  
Island Populations ◽  
Svalbard Reindeer

Polyacrylamide gel electrophoresis was used to analyse transferrin variation in caribou from the Canadian Arctic islands. Sixteen alleles were detected in Peary caribou (Rangifer tarandus pearyi). The most common allele was TfG2, which increased in frequency from 0.167 at the Boothia Peninsula to 0.236 in the Peel population and 0.340 in the Parry population. The presence of this allele, which is the most common allele in Svalbard reindeer (R. t. platyrhynchus) and not detected in Norwegian reindeer (R. t. tarandus), suggests a common origin for the Peary caribou and the Svalbard reindeer. The large genetic distance in the transferrin locus between continental and island populations suggests the isolation of a High Arctic population in a northern refugium during the Wisconsin glaciation.

Polyacrylamide-Gel Disc-Electrophoresis as a Screening Procedure for Serum Lipoprotein Abnormalities

1973 ◽  
Vol 19 (2) ◽  
pp. 228-234 ◽  
Author(s):  
Herbert K Naito ◽  
Mitsuo Wada ◽  
L A Ehrhart ◽  
Lena A Lewis
Keyword(s):  
Gel Electrophoresis ◽  
Analytical Ultracentrifugation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Screening Method ◽  
Serum Lipoprotein ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Electrophoretic Method ◽  
Wide Range ◽  
Electrophoresis System

Abstract A polyacrylamide-gel disc-electrophoresis system is described that is suitable for screening sera with a wide range of lipid concentrations, and the results agree with analytical ultracentrifugation data. The lipoprotein pattern so obtained was compared with that obtained by other recognized electrophoretic procedures. Lipoprotein determinations were done by polyacrylamide gel electrophoresis, for 125 patients, to determine its merit as a clinical screening method for lipoprotein abnormalities as compared to the paper-electrophoretic method. The lipid dye and riboflavin concentration of the gels used give a background that makes it easy to detect chylomicra in the sample gel and allows for densitometric semiquantitation of the lipoprotein fractions. The method is reliable, precise, and accurate.

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