Genetic Transformation of Woody Plants Using Embryogenic Cultures

2005 ◽  
Vol 7 (2) ◽  
pp. 17-35 ◽  
Author(s):  
P. Suprasanna ◽  
T. R. Ganapathi ◽  
V. A. Bapat
Keyword(s):  
Genetic Transformation ◽  
Woody Plants ◽  
Embryogenic Cultures

Stable genetic transformation of embryogenic cultures of Abies nordmanniana (nordmann fir) and regeneration of transgenic plants

2005 ◽  
Vol 41 (6) ◽  
pp. 725-730 ◽  
Author(s):  
Jens I. Find ◽  
Julia A. Charity ◽  
Lynette J. Grace ◽  
Michel M. M. H. Kristensen ◽  
Peter Krogstrup ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Embryogenic Cultures ◽  
Abies Nordmanniana ◽  
Nordmann Fir

scholarly journals Genetic Transformation of Populus trichocarpa Genotype Nisqually-1: A Functional Genomic Tool for Woody Plants

2006 ◽  
Vol 47 (11) ◽  
pp. 1582-1589 ◽  
Author(s):  
Jingyuan Song ◽  
Shanfa Lu ◽  
Zenn-Zong Chen ◽  
Rodrigo Lourenco ◽  
Vincent L. Chiang
Keyword(s):  
Genetic Transformation ◽  
Woody Plants ◽  
Populus Trichocarpa ◽  
Functional Genomic ◽  
Genomic Tool

scholarly journals Micropropagation Cultures for Genetic Transformation of Grapevine

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 972C-972
Author(s):  
Manjul Dutt ◽  
Dennis J. Gray ◽  
Zhijian T. Li ◽  
Sadanand Dhekney ◽  
Marilyn M. Van Aman
Keyword(s):  
Genetic Transformation ◽  
Fluorescent Protein ◽  
Marker Gene ◽  
In Vitro Cultures ◽  
35S Promoter ◽  
Nptii Gene ◽  
Embryogenic Cultures ◽  
Wide Range ◽  
Over Time

A major drawback to the use of embryogenic cultures for transformation of grapevine is that their ability to undergo genetic transformation is cultivar-dependent. Also, depending on cultivar, embryogenic cultures are difficult to impossible to maintain over time, reducing their utility for use in genetic transformation. An alternative to the use of embryogenic cultures for transformation of grapevine is the use of micropropagation cultures, which are easier to initiate from a wide range of grapevine cultivars and can be maintained over time without loss of function. Vitis vinifera `Thompson Seedless' was used as a model for genetic transformation using micropropagation cultures. In vitro cultures were initiated from apical meristems of actively growing vines and maintained in C2D medium containing 4 μM of 6-benzylaminopurine (C2D4B). Shoot tips and nodes were collected from proliferating in vitro cultures for transformation studies. A variety of wounding techniques, including nicking, sonication, and fragmenting of meristematic tissues was employed in order to enable Agrobacterium infection. We used a construct containing a bidirectional 35S promoter complex with a marker gene composed of a bifunctional fusion between an enhanced green fluorescent protein (EGFP) gene and a neomycin phosphotransferase (NPTII) gene in one direction and a hybrid lytic peptide gene in the other. Transgenic shoots growing in C2D4B medium containing 200 mg·L-1 each of carbenicillin and cefotaxime and 20 mg·L-1 of kanamycin were selected based on GFP fluorescence. Transgenic shoots were rooted and transferred to a greenhouse. To date, 18 transgenic lines have been generated. Details on the transformation procedure will be discussed.

scholarly journals Efficient Agrobacterium-Mediated Transformation of the Commercial Hybrid Poplar Populus Alba × Populus glandulosa Uyeki

2019 ◽  
Vol 20 (10) ◽  
pp. 2594 ◽  
Author(s):  
Chengwei Song ◽  
Liang Lu ◽  
Yayu Guo ◽  
Huimin Xu ◽  
Ruili Li
Keyword(s):  
Genetic Transformation ◽  
Woody Plants ◽  
Functional Characterization ◽  
Hybrid Poplar ◽  
Leaf Explants ◽  
Populus Alba ◽  
Gus Gene ◽  
Transgenic Technology ◽  
Transformation System ◽  
Agrobacterium Mediated Transformation

Transgenic technology is a powerful tool for gene functional characterization, and poplar is a model system for genetic transformation of perennial woody plants. However, the poplar genetic transformation system is limited to a number of model genotypes. Herein, we developed a transformation system based on efficient Agrobacterium-mediated transformation for the hybrid poplar Populus Alba × Populus glandulosa Uyeki, which is a fast-growing poplar species that is suitably grown in the northern part of China. Importantly, we optimized many independent factors and showed that the transformation efficiency was improved significantly using juvenile leaf explants. Explants were infected by an Agrobacterium suspension with the OD600 = 0.6 for 15 min and then co-cultured in dark conditions for 3 days. Using the improved transformation system, we obtained the transgenic poplar with overexpression of β-glucuronidase (GUS) via direct organogenesis without callus induction. Furthermore, we analyzed the GUS gene in the transgenic poplars using PCR, qRT-PCR, and GUS staining. These analyses revealed that the GUS gene was efficiently transformed, and it exhibited various expression levels. Taken together, these results represent a simple, fast, and efficient transformation system of hybrid poplar plants. Our findings may facilitate future studies of gene functions in perennial woody plants and tree breeding via transgenic technology assisted design.

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba
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