Stable genetic transformation of embryogenic cultures of Abies nordmanniana (nordmann fir) and regeneration of transgenic plants

2005 ◽  
Vol 41 (6) ◽  
pp. 725-730 ◽  
Author(s):  
Jens I. Find ◽  
Julia A. Charity ◽  
Lynette J. Grace ◽  
Michel M. M. H. Kristensen ◽  
Peter Krogstrup ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Embryogenic Cultures ◽  
Abies Nordmanniana ◽  
Nordmann Fir

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

1997 ◽  
Vol 24 (1) ◽  
pp. 97 ◽  
Author(s):  
K. Kazan ◽  
M. D. Curtis ◽  
K. C. Goulter ◽  
J. M. Manners
Keyword(s):  
Brassica Napus ◽  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Double Haploid ◽  
Gene Promoter ◽  
Root Explants ◽  
Hypocotyl Explants ◽  
Peroxidase Gene ◽  
Hypocotyl Segments

Double haploid (DH) genotypes of canola (Brassica napus L.) have a high level of genetic uniformity but have not been previously tested for genetic transformation. Transgenic plants from three of four DH genotypes derived from cv. Westar were obtained by inoculation of either hypocotyl segments or root explants with Agrobacterium tumefaciens. For hypocotyl transformation, A. tumefaciens strain LBA4404 containing a binary plasmid with the neomycin phosphotransferase gene (nptII) and a CaMV 35S-peroxidase gene cassette was co-cultivated with hypocotyl segments taken from the 5–6-day-old seedlings. Transformation frequencies for hypocotyl explants of two DH genotypes were 0.3–3%. Direct evidence for genetic transformation of hypocotyl explants was obtained through molecular hybridisation analysis. Using this protocol, mature transformed plants were obtained within 4–6 months of co-cultivation. A method of root transformation was successfully modified for one DH genotype of canola and transgenic plants were obtained at a frequency of 2%. Using this protocol, a peroxidase gene promoter–GUS fusion construct was introduced into a DH genotype. Tissue specific GUS expression driven by the peroxidase gene promoter in transgenic plants was analysed by GUS staining. Transformation systems for double haploid canola lines will permit the assessment of introduced genes for their effect on agronomic and physiological traits.

Genetic Transformation of Woody Plants Using Embryogenic Cultures

2005 ◽  
Vol 7 (2) ◽  
pp. 17-35 ◽  
Author(s):  
P. Suprasanna ◽  
T. R. Ganapathi ◽  
V. A. Bapat
Keyword(s):  
Genetic Transformation ◽  
Woody Plants ◽  
Embryogenic Cultures

Genetic transformation of sweet passion fruit (Passiflora alata) and reactions of the transgenic plants to Cowpea aphid borne mosaic virus

2015 ◽  
Vol 143 (4) ◽  
pp. 813-821 ◽  
Author(s):  
Marcelo Favareto Correa ◽  
Ana Paula Chiaverini Pinto ◽  
Jorge Alberto Marques Rezende ◽  
Ricardo Harakava ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Mosaic Virus ◽  
Passion Fruit ◽  
Cowpea Aphid

Genetic transformation of citrus protoplasts and regeneration of transgenic plants

Plant Science ◽  
1990 ◽  
Vol 69 (2) ◽  
pp. 199-206 ◽  
Author(s):  
Aliza Vardi ◽  
Shlomit Bleichman ◽  
Dvora Aviv
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation

scholarly journals Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

2021 ◽  
Vol 3 (1) ◽  
pp. 01-05
Author(s):  
Malik Shuja
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Selectable Marker ◽  
Normal Growth ◽  
Minimal Effect ◽  
Effective Screening ◽  
Transformed Plants ◽  
Transformation Systems ◽  
Selection Agent ◽  
Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

scholarly journals GENETIC TRANSFORMATION AND REGENERATION OF TRANSGENIC SWEETPOTATO PLANTS.

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 435f-435 ◽  
Author(s):  
Marceline Egnin ◽  
C.S. Prakash
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Binary Vector ◽  
Blot Hybridization ◽  
Pcr Amplification ◽  
Ms Medium ◽  
Petiole Explants ◽  
Efficient Delivery ◽  
Foreign Genes

This study aimed to optimize factors for the efficient delivery of foreign genes into sweetpotato using Agrobacterium tumefaciens and develop transgenic plants. Disarmed Agrobacterium C58 carrying a binary vector pBI 121C2H with gusA, nptll, and the nutritional protein asp-l genes was used to cocultivate (4 days) petiole explants of the sweetpotato genotype P1318846-3. Pre-incubation of petioles for 3 days on MS medium with 2,4-D (0.2 mg·liter–1) before infection resulted in higher transformation. Putative transgenic shoots were obtained by transfer of petioles to MS medium with TDZ (0.2 mg·liter–1) and kanamycin (80 to 140 mg·liter–1). The PCR amplification of gusA, nptll, and asp-1 genes in the 37 putative transgenic shoots showed that six plants contained the three genes. However, none of these plants showed histochemical expression of the gusA gene. The introduced gene may have been methylated resulting in the lack of its expression. DNA blot hybridization studies are underway to verify the presence and integration of the transgenes.

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