scholarly journals Germination and Seedling Growth of Pulse Crop (Vigna Spp.) as Affected by Soil Salt Stress

2016 ◽  
Vol 4 (2) ◽  
pp. 159-170 ◽  
Author(s):  
Pankaj Awasthi ◽  
Himani Karki ◽  
Vibhuti Vibhuti ◽  
Kiran Bargali ◽  
S. S Bargali
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Growth Stages ◽  
Sensitive Species ◽  
Worldwide Distribution ◽  
Vigna Species ◽  
Stress Levels

Vigna is a genus of flowering plants in the legume family, Fabaceae with worldwide distribution. In the present study, effect of salt stress was investigated in four species of Vigna viz. V. mungo (urd), V. angularis (rais), V. radiata (moong) and V. aconitifolia (moth) at germination and seedling growth stages in a glasshouse experiment. Seeds of selected crops were surface sterilized and placed under three salt stress levels of 0 (control), 50mM and 100mM using NaCl solutions. Three replicates for each treatment was used for this experiment. The results indicated that increase in salt stress levels caused a reduction in germination percentage, germination rate, shoot length, root length and seedling dry weight of each Vigna species. The overall results indicated that among the four species, V. aconitifolia was the most sensitive, whereas V. mungo was the least sensitive species to the salinity in almost every aspect of growth.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

scholarly journals Responses of Maize Varieties to Salt Stress in Relation to Germination and Seedling Growth

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Nuurismaan Hassan ◽  
Md. Kamrul Hasan ◽  
Md. Obaidullah Shaddam ◽  
Mohammad Sohidul Islam ◽  
Celaleddin Barutçular ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Agricultural Research ◽  
Growth Parameters ◽  
Tap Water ◽  
Germination Rate ◽  
Dry Weight ◽  
Growth Stages ◽  
Water Control ◽  
Maize Varieties

A pot experiment was carried out at the Laboratory of Department of Agronomy, Hajee Mohamad Danesh Science and Technology University (HSTU), Bangladesh during 2016 to evaluate the response of maize varieties at germination and seedling growth stages under salt stress. The seeds of the BARI (Bangladesh Agricultural Research Institute) developed four maize varietiesviz.Barnali, Khoi Vutta, Mohor and BARI Maize 5 were placed in plastic pots (each of 25 cm length and 12 cm width) on sand bed irrigated with tap water (control), 100 and 200 mM NaCl salt solutions. It was replicated in thrice with completely randomized design (CRD). Salinity stress significantly affected the germination characters and seedling growth parameters of maize varieties. The germination percentages (GP) and germination rate (GR) reduced significantly with increasing salinity, and the variety Khoi Vutta showed the highest GP and GR followed by Barnali and Mohor showed the lowest GP and GR followed by BARI maize 5. Under high salinity level, seedling growths characteristics like shoot and root lengths, fresh and dry weight of shoot and roots reduced remarkably in the variety Mohor indicating salt susceptible while the minimum reduction of the aforementioned traits was observed in the variety Khoi Vutta demonstrating high salt tolerant variety. The studied varieties can be ranked on the basis of salt tolerance as Khoi Vutta > Barnali > BARI Maize 5 > Mohor from the experiment.

scholarly journals Determination of germination and seedling growth parameters of rice (Oryza sativa L.) varieties under stress conditions

2020 ◽  
Vol 12 (3) ◽  
pp. 693-701
Author(s):  
Fatih ÖNER ◽  
Ayşe Ö. Ş. SOYSAL
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Growth Parameters ◽  
Oryza Sativa L ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Abiotic Factor ◽  
Rice Varieties ◽  
Stress Levels

Rice (Oryza sativa L.) is an important food for more than half of the world’s population. Globally, rice is grown on approximately 160 million hectares with an average annual production of 740.96 million tonnes. Salinity is an important abiotic factor for germination. In this study rice varieties were analysed for salt stress tolerance at germination growth stage. The response of eight rice varieties against six (0, 25, 50, 75, 100, 150 mM NaCl) salinity levels were studied at germination stage. Seeds of eight rice varieties (‘Yatkin’, ‘Miss-2013 Passali’, ‘Kale’, ‘Manyas Yildizi’, ‘Efe’, ‘Biga Incisi’ and ‘Osmancik-97’) were kept under six salt stress levels. Number of seeds germinated (number), germination rate (%), average germination time, fresh and dry weight of germinated seeds (g), fresh and dry weight of the coleoptile (g), fresh and dry weight of the radicle (g), the length of the coleoptile and radicle (mm) were recorded. The results showed that with increasing salt stress, germination in all the varieties was delayed. The increase in salt stress also reduced every measured trait significantly in all the varieties. Maximum germination percentage (100%) was observed in ‘Biga Incisi’ under all the salt stress levels. Our research data would be helpful for identification of the tolerant varieties which can be studied further in terms of economically.

scholarly journals Effects of NaCl on Germination and Seedling Growth in Macrotyloma uniflorum and Vigna mungo

2017 ◽  
Vol 5 (2) ◽  
pp. 169-176 ◽  
Author(s):  
SUMAN PANTOLA ◽  
KIRAN BARGALI ◽  
S.S. BARGALI ◽  
VIBHUTI VIBHUTI
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Vigna Mungo ◽  
Growth Stages ◽  
Tolerance Index ◽  
Abiotic Factor ◽  
Weight Percentage ◽  
Macrotyloma Uniflorum

Among abiotic stresses, drought and salinity are two major determinants due to high magnitude of their impact and wide occurrence. Salinity considerably limits the productivity of crops and thus, considered as the most destructive abiotic factor. In the present study, response of Macrotyloma uniflorum and Vigna mungo to salt (NaCl) stress imposed at germination and seedling growth stages was investigated. The aim of the study was identify the physiological and morphological responses of selected leguminous crop. Seeds were obtained from the healthy plants, surface sterilized and placed under six salt stress levels (0, 4, 8, 12, 16, 20 dsm-1). Complete randomized design with three replicates was used for this experiment and the experiments were conducted during the year 2015 in the glasshouse of Department of Botany, DSB, Campus, Kumaun University, Nainital. In comparison to Macrotyloma uniflorum (77%), higher germination percentage was observed in Vigna mungo (99%) at all salinity levels. In both the species, germination percentage and seedling growth decreased with the increase in salinity stress. Though Vigna mungo showed higher values for root (0.17 g) and shoot dry mass (0.27 g) the dry weight percentage reduction was higher in this species as compared to Macrotyloma uniflorum. Decrease in biomass of seedling with increasing salt stress indicated that the stress not only affected germination but also the growth of seedlings, which indicates that the synthetic ability of seed and biomass of the seedlings was also affected. Seed vigor index declined with the increase in salt concentrations. Outcomes from the study could be helpful in understanding the plant’s nature against different levels of salt stress and that could be economically exploited by various able agencies. At each salinity level, M. uniflorum showed higher salt tolerance index as compared to V. mungo so it can be cultivated in fields with salty soil.

scholarly journals Genetic variability of red clover seedlings in relation to salt stress

2014 ◽  
Vol 30 (3) ◽  
pp. 529-538 ◽  
Author(s):  
V. Mandic ◽  
V. Krnjaja ◽  
Z. Bijelic ◽  
Z. Tomic ◽  
A. Simic ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Red Clover ◽  
Dry Weight ◽  
Growth Stages ◽  
Early Seedling Growth ◽  
Vigor Index ◽  
Growing Medium ◽  
Early Seedling ◽  
Sterile Plastic

Red clover is highly salt-sensitive plant, especially during germination and early seedling growth stages. The aim of this investigation was to estimate the effects of different saline conditions (0, 50, 100, 150, and 200mM NaCl) on germination and early seedling growth in four red clover varieties (Kolubara, K-32, K-17 and K-39). Germination test was conducted in the laboratory conditions using sterile plastic vessels on filter paper moistened with 10ml of the appropriate salt test solutions. It was observed that the germination energy (GE), germination (G), percentage of dead or infected seeds (DIS), normal seedlings (NS), root length (RL), shoot length (ShL), fresh weight (FW) and dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium. The tested varieties of red clover showed different NaCl tolerance at the seedling stage. Generally, studied red clover varieties are very sensitive to salt, especially K-32 which has the lowest values for GE, G, NS and SVI and highest for DIS. Variety K-17 proved to be a variety that the best tolerates conditions of salt stress because the values for GE, G, NS, RL and SVI were highest. Testing of varieties of red clover in the early seedling growth at different concentrations of NaCl in the growing medium could be helpful in the identification and selection of varieties for cultivation on saline soils.

scholarly journals Allelopathy of Sage and White Wormwood on Purslane Germination and Seedling Growth

2010 ◽  
Vol 2 (3) ◽  
pp. 91-95 ◽  
Author(s):  
Alireza PIRZAD ◽  
Vahid GHASEMIAN ◽  
Reza DARVISHZADEH ◽  
Mohammad SEDGHI ◽  
Abbas HASSANI ◽  
...  
Keyword(s):  
Seedling Growth ◽  
Untreated Control ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Root Shoot Ratio ◽  
Root And Shoot Length ◽  
Length Changes ◽  
White Wormwood

A bioassay run was carried out in the incubator to evaluate possible allelopathic effects of water extracts of sage and white wormwood on germination and seedling growth of purslane. Results showed that the type of extract and its concentration (0, 5, 10, 15 and 20%) significantly influenced final germination percentage, germination rate and index, root and shoot length, root/shoot ratio, fresh and dry weight of seedling of purslane. The interaction between these two experimental factors was always significant, producing different results according to the different combination levels. The statistical comparison of means indicated that the maximum germination percentage and germination rate (respectively 68% and 11.4% d-1) were obtained from the untreated control (0% extract), while the lowest values for the same two characters occurred with 15% of sage (respectively 45% and 6.4% d-1) and 20% of white wormwood (38% and 7.5% d-1). Concerning germination indexes, the highest (55) and the lowest (32) values were observed respectively on the control and with 15% of sage extract or 20% of white wormwood extract. The longest (4.5 cm) and the shortest (1.3 cm) root of purslane were obtained with 5% of white wormwood and 20% of sage extracts, respectively. Changes in shoot length with sage and white wormwood extracts were similar to those in root length, even though shoot length was less affected by the concentration of extracts. Root and shoot length changes brought to maximum (22.0) and minimum (8.9) values for the root/shoot length ratio, respectively with 5% of white wormwood and 20% of sage extracts. The maximum fresh (2.111 g) and dry (0.338 g) seedling weight of purslane were obtained from untreated control, producing the same seedling weight with 5% of sage, 5 and 10% of white wormwood extract. While the minimum fresh (0.692 g) and dry (0.111 g) seedling weight were obtained from 15% of white wormwood extract, so that there is no significant differences between this value and the seedling weight produced under 10, 15 and 20% of sage and 20% of white wormwood extract.

scholarly journals Germination and Seedling Growth of Zea mays L. under Different Levels of Sodium Chloride Stress

2014 ◽  
Vol 12 ◽  
pp. 5-15 ◽  
Author(s):  
R. Sozharajan ◽  
S. Natarajan
Keyword(s):  
Zea Mays ◽  
Salt Stress ◽  
Water Uptake ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Germination Rate ◽  
Biomass Accumulation ◽  
Petri Dish ◽  
Germination Percentage ◽  
Yield Reduction

Salt stress is one of the most severe environmental factors that reduces and limits growth and development of plants. Abiotic stresses such as heavy metals, salinity, drought, temperature, UV-radiation, ozone causes drastic yield reduction in most of the crops. Especially salt stress affects around 20 of NaCl on germination and seedling growth of Zea mays L. Seeds of Zea mays were germinated in glass Petri- 100 × 15 mm diameter lined with blotting paper. Ten seeds were placed in each petri-dish. Petridishes were irrigated with 25, 50, 75, 100, 125, 150, 175 and 200 mM concentrations of NaCl. A control was moistened with ten milliliters of distilled water. The germination percentage, water absorption of the seeds, water uptake percentage and the growth parameters were observed. The results obtained showed that the inhibition of the germination percentage, germination rate, water uptake, growth and biomass accumulation of the seedlings were observed to decrease with increasing NaCl concentrations. At the highest level of stress both plumule and radical decreased significantly. The salt stress decreased seed germination, biomass and growth of maize seedlings due to ion toxicity, decrease osmotic potential and oxidative stress

scholarly journals The Efficiency of Different Priming Agents for Improving Germination and Early Seedling Growth of Local Tunisian Barley under Salinity Stress

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2264
Author(s):  
Rim Ben Youssef ◽  
Nahida Jelali ◽  
Nadia Boukari ◽  
Alfonso Albacete ◽  
Cristina Martinez ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Electrolyte Leakage ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Nutrient Content ◽  
Hordeum Vulgare L ◽  
Membrane Injury ◽  
Essential Nutrient

The current work aimed to investigate the effect of seed priming with different agents (CaCl2, KCl, and KNO3) on germination and seedling establishment in seeds of the barley species of both Hordeum vulgare (L. Manel) and Hordeum maritimum germinated with three salt concentrations (0, 100, and 200 mM NaCl). The results showed that under unprimed conditions, salt stress significantly reduced the final germination rate, the mean daily germination, and the seedling length and dry weight. It led to a decrease in the essential nutrient content (iron, calcium, magnesium, and potassium) against an increase in sodium level in both of the barley species. Moreover, this environmental constraint provoked a membrane injury caused by a considerable increase in electrolyte leakage and the malondialdehyde content (MDA). Data analysis proved that seed priming with CaCl2, KCl, and KNO3 was an effective method for alleviating barley seed germination caused by salt stress to varying degrees. Different priming treatments clearly stimulated germination parameters and the essential nutrient concentration, in addition to increasing the seedling growth rate. The application of seed priming reduced the accumulation of sodium ions and mitigated the oxidative stress of seeds caused by salt. This mitigation was traduced by the maintenance of low levels of MDA and electrolyte leakage. We conclude that the priming agents can be classed into three ranges based on their efficacy on the different parameters analyzed; CaCl2 was placed in the first range, followed closely by KNO3, while the least effective was KCl, which placed in the third range.

scholarly journals First Report of Sesame Mutants Tolerant to Severe Drought Stress during Germination and Early Seedling Growth Stages

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1166
Author(s):  
Mohamed Kouighat ◽  
Hafida Hanine ◽  
Mohamed El Fechtali ◽  
Abdelghani Nabloussi
Keyword(s):  
Drought Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Percentage ◽  
Growth Stages ◽  
Severe Drought ◽  
First Report ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Sesame Genotypes

In the context of climate change and water scarcity, there is a need to develop and use drought-tolerant sesame cultivars. This study was conducted to evaluate the response of 13 sesame genotypes, including 11 mutants and their wild-types, to drought during germination and early seedling growth. Moderate and severe drought stress was simulated by applying polyethylene glycol (PEG) at two osmotic potentials, −0.6 MPa and −1.2 MPa, respectively, on seeds of two successive mutant generations, M2 and M3. The parameters measured or calculated were germination percentage (GP), germination rate (GR), mean germination time (MGT), root length (RL), shoot length (SL), root to shoot ratio (RSR), and the seedling vigor index (SVI). Results showed the significant effect of genotype, drought, and drought × genotype interaction on all parameters investigated. Under severe drought, seeds of seven genotypes, including wild types, were not able to germinate. There was a drastic decline of all parameters for the rest, except MGT and RSR, which markedly increased. Interestingly, two mutants, “ML2-5” and “ML2-10”, were identified as the most tolerant to severe drought and the most stable over both generations. The present work is the first report of sesame germplasm with such a high level of tolerance to drought during germination and early seedling growth stages.

Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress

2007 ◽  
Vol 58 (8) ◽  
pp. 811 ◽  
Author(s):  
S. Zhang ◽  
J. Hu ◽  
Y. Zhang ◽  
X. J. Xie ◽  
Allen Knapp
Keyword(s):  
Salt Stress ◽  
Medicago Sativa ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Shoot Dry Weight ◽  
Medicago Sativa L ◽  
High Level

Salt stress is an important constraint to lucerne (Medicago sativa L.) production in many parts of the world. Seeds of 3 lucerne varieties, cvv. Victoria, Golden Empress, and Victor, were used to investigate the effects of seed priming with 5 µm/L brassinolide on germination and seedling growth under a high level of salt stress (13.6 dS/m NaCl solution). The results showed that germination percentage, germination index, and vigour index of lucerne seeds primed with brassinolide were significantly higher than those of the non-primed seeds under salinity stress in each variety. Seed priming with brassinolide significantly increased the shoot fresh weight, shoot dry weight, and root dry weight in 2 varieties, and significantly increased the root length and root vigour in each variety. It also significantly increased the activities of antioxidant enzymes, peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT), in Victoria and Victor seedlings. During seedling growth, the primed seeds significantly reduced the malondialdehyde (MDA) accumulation. This suggests that priming lucerne seed with brassinolide at a suitable concentration can improve germination and seedling growth under high-saline soils.

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