scholarly journals The Influence of Pre- and Post-natal Exposure to Methoxychlor on the Immune System and Renal Function of Sprague-Dawley Rats

2005 ◽  
Vol 18 (2) ◽  
pp. 89-98
Author(s):  
Yukiko Takeuchi ◽  
Naofumi Takahashi ◽  
Tadashi Kosaka ◽  
Koichi Hayashi ◽  
Yuko Chiba ◽  
...  
Keyword(s):  
Renal Function ◽  
Immune System ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Effects of TRPC6 Inactivation on Glomerulosclerosis and Renal Fibrosis in Aging Rats

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 856
Author(s):  
Eun Young Kim ◽  
Stuart E. Dryer
Keyword(s):  
Renal Function ◽  
Protective Effect ◽  
Renal Fibrosis ◽  
Transient Receptor Potential ◽  
Smooth Muscle Actin ◽  
Receptor Potential ◽  
Sprague Dawley ◽  
Age Related ◽  
Sprague Dawley Rats ◽  
Transient Receptor

Canonical transient receptor potential 6 (TRPC6) channels have been implicated in familial and acquired forms of focal and segmental glomerulosclerosis (FSGS) in patients and animal models, as well as in renal fibrosis following ureteral obstruction in mice. Aging also evokes declines in renal function owing to effects on almost every renal compartment in humans and rodents. Here, we have examined the role of TRPC6 in driving inflammation and fibrosis during aging in Sprague-Dawley rats. This was assessed in rats with non-functional TRPC6 channels owing to CRISPR-Cas9 deletion of a portion of the ankyrin repeat domain required for the assembly of functional TRPC6 channels (Trpc6del/del rats). Wild-type littermates (Trpc6wt/wt rats) were used as controls. Animals were evaluated at 2 months and 12 months of age. There was no sign of kidney disease at 2 months of age, regardless of genotype. However, by 12 months of age, all rats examined showed declines in renal function associated with albuminuria, azotemia and increased urine excretion of β2–microglobulin, a marker for proximal tubule pathology. These changes were equally severe in Trpc6wt/wt and Trpc6del/del rats. We also observed age-related increases in renal cortical expression of markers of fibrosis (α-smooth muscle actin and vimentin) and inflammation (NLRP3 and pro-IL−1β), and there was no detectable protective effect of TRPC6 inactivation. Tubulointerstitial fibrosis assessed from histology also appeared equally severe in Trpc6wt/wt and Trpc6del/del rats. By contrast, glomerular pathology, blindly scored from histological sections, suggested a significant protective effect of TRPC6 inactivation, but only within the glomerular compartment.

Hesperidin Shows Protective Effects on Renal Function in Ischemia-induced Acute Kidney Injury (Sprague-Dawley Rats)

2019 ◽  
Vol 51 (8) ◽  
pp. 2838-2841
Author(s):  
Won Seo Park ◽  
Min Su Park ◽  
Sang Wook Kang ◽  
Seul A. Jin ◽  
Youngchul Jeon ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Renal Function ◽  
Kidney Injury ◽  
Protective Effects ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Evidences Suggesting that Distinct Immunological and Cellular Responses to Light Damage Distinguishes Juvenile and Adult Rat Retinas

2019 ◽  
Vol 20 (11) ◽  
pp. 2744
Author(s):  
Anna Polosa ◽  
Shasha Lv ◽  
Wassila Ait Igrine ◽  
Laura-Alexie Chevrolat ◽  
Hyba Bessaklia ◽  
...  
Keyword(s):  
Immune System ◽  
Light Exposure ◽  
Age Related Macular Degeneration ◽  
Light Damage ◽  
Sprague Dawley ◽  
Adult Rat ◽  
Age Related ◽  
Degenerative Disorders ◽  
Sprague Dawley Rats ◽  
Dose Dependent

To unravel the mechanisms behind the higher resistance to light damage of juvenile (JR) versus adult (AR) rats, Sprague Dawley rats were exposed to a bright luminous environment of 10, 000 lux. The light-induced retinopathy (LIR) was assessed with histology, electroretinography and immunohistochemistry (IHC). In JR, 2 days of exposure induced the typical LIR, while >3 days added little LIR. IHC revealed a subtle migration of microglia (Iba1 marker) from the inner to the outer retina after 3 days of exposure in JR contrasting with the stronger reaction seen after 1 day in AR. Similarly, in JR, the Müller cells expressed less intense GFAP, CNTF and FGF2 staining compared to AR. Our results suggest that in JR the degree of retinal damage is not proportional to the duration of light exposure (i.e., dose-independent retinopathy), contrasting with the dose-dependent LIR reported in AR. The immature immune system in JR may explain the delayed and/or weaker inflammatory response compared to AR, a finding that would also point to the devastating contribution of the immune system in generating the LIR phenotype, a claim also advanced to explain the pathophysiology of other retinal degenerative disorders such as Age-related Macular Degeneration, Diabetic Retinopathy and Retinitis Pigmentosa.

Short-Term Effects of Titanium Dioxide Nanofiber on the Renal Function of Male Sprague Dawley Rats

2018 ◽  
Vol 37 (2) ◽  
pp. 127-138 ◽  
Author(s):  
Daniel A. Hunter ◽  
Leah K. Bartel ◽  
Ian Byrd ◽  
Bethany Bogan ◽  
Wilson Yau ◽  
...  
Keyword(s):  
Renal Function ◽  
Titanium Dioxide ◽  
Sprague Dawley ◽  
Short Term ◽  
Sprague Dawley Rats ◽  
Short Term Effects

scholarly journals Heme Induction with Delta-Aminolevulinic Acid Stimulates an Increase in Water and Electrolyte Excretion

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Syed Quadri ◽  
Debra W. Jackson ◽  
Priyanka Prathipati ◽  
Courtney Dean ◽  
Keith E. Jackson
Keyword(s):  
Carbon Monoxide ◽  
Renal Function ◽  
Aminolevulinic Acid ◽  
Acute Administration ◽  
Bolus Administration ◽  
Sprague Dawley ◽  
Electrolyte Excretion ◽  
Sprague Dawley Rats ◽  
Before And After

Purpose. Studies were performed to examine hemodynamic and renal function before and after acute induction of the endogenous CO system with delta-aminolevulinic acid (DALA), which drives HO activity.Methods.In vivostudies were conducted on Inactin-anesthetized male Sprague Dawley rats (250–300 g) either with or without chronic pretreatment with L-NAME (50 mg/Kg, q12 hours x4d).Results. DALA (80 μmol/Kg, IV bolus) administration acutely increased endogenous CO production and HO-1 protein. In untreated and L-NAME-pretreated rats, DALA did not alter BP, GFR, or RBF but increased UF,UNaV, andUKV(untreated: Δ108.8 ± 0.28%, 172.1 ± 18.4%, and 165.2 ± 45.9%; pretreated: Δ109.4 ± 0.29%, 187.3 ± 26.9%, and 197.2 ± 45.7%). Acute administration of biliverdin (20 mg/kg, IV) and bilirubin (30 mg/kg, IV) to similarly treated animals did not alter UF,UNaV, andUKV.Conclusion. These results demonstrate that heme oxygenase induction increases urine and electrolyte excretion and suggest a direct tubular action of endogenous carbon monoxide.

scholarly journals Effects of Fumonisin B1 on the Immune System of Sprague-Dawley Rats Following a 14-Day Oral (Gavage) Exposure

1997 ◽  
Vol 39 (1) ◽  
pp. 53-59
Author(s):  
H. Tryphonas ◽  
G. Bondy ◽  
J. D. Miller ◽  
F. Lacroix ◽  
M. Hodgen ◽  
...  
Keyword(s):  
Immune System ◽  
Fumonisin B1 ◽  
Sprague Dawley ◽  
Oral Gavage ◽  
Sprague Dawley Rats

scholarly journals Effect of different sweeteners on the oral microbiota and immune system of Sprague Dawley rats

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xi Cheng ◽  
Xiurong Guo ◽  
Feihong Huang ◽  
Hui Lei ◽  
Quan Zhou ◽  
...  
Keyword(s):  
Immune System ◽  
High Throughput Sequencing ◽  
Pure Water ◽  
Structure And Function ◽  
Oral Microbiome ◽  
Oral Microbiota ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
And Function

AbstractSucrose, xylose, and saccharin are commonly used beverage additives and long-term consumption of these compounds inevitably affects the oral immune system and the composition of oral microbiomes. In this study, we used 24 Sprague Dawley rats divided into four groups, i.e., sucrose, saccharin, xylose, or pure water treated over an eight week period to evaluate any changes in the composition, community structure, and function of the oral microbiomes. At the end of the treatment period, we collected oral microbiome samples from each animal and subjected them to high-throughput sequencing. We also used ELISA to determine the concentration of salivary immunoglobulin in these rats to reveal the effect of sweetener on the oral immune system. Sequencing results demonstrated that Firmicutes and Proteobacteria, remained the predominant phyla, but we found that the oral microbial diversity of rats drinking sucrose water was significantly higher than that of the other groups. Our results indicate that drinking water supplemented with sweeteners may influence oral immunity as well as the composition, metabolic function, and diversity of the oral microbiota, thereby disrupting the oral microbiome.

scholarly journals The Role of the Immune System in Nevirapine-Induced Subclinical Liver Injury of a Rat Model

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Zanelle Bekker ◽  
Andrew Walubo ◽  
Jan B. du Plessis
Keyword(s):  
Immune System ◽  
Liver Injury ◽  
Rat Model ◽  
Chronic Phase ◽  
Therapeutic Strategies ◽  
Cell Swelling ◽  
Sprague Dawley ◽  
Immune Parameters ◽  
Sprague Dawley Rats

In this study, the role of the immune system in nevirapine- (NVP-) induced subclinical liver injury was investigated by observing for changes of some immune parameters during the initial stages of NVP-induced hepatotoxicity in a rat model. In the acute phase, two test-groups of 10 Sprague-Dawley rats each were administered with bacterial lipopolysaccharide (LPS) or saline (S) intraperitoneally, followed by oral NVP, after which 5 rats from each group were sacrificed at 6 and 24 hours. For the chronic phase, two groups of 15 rats each received daily NVP, and on days 7, 14, and 21, five rats from each group were administered with either LPS or S, followed by that day’s NVP dose, and were sacrificed 24 hours later. NVP caused liver injury up to seven days and progressively increased IL-2 and IFN-γ levels and lymphocyte count over the 21 days. NVP-induced liver injury was characterized by apoptosis and degeneration changes, while, for LPS, it was cell swelling, leukostasis, and portal inflammation. Coadministration of NVP and LPS attenuated NVP-induced liver injury. In conclusion, the immune system is involved in NVP toxicity, and the LPS effects may lay the clue to development of therapeutic strategies against NVP-induced hepatotoxicity.

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