scholarly journals Evaluation of a Microplate Agglutination Test (MAT) for Serological Diagnosis of Canine Brucellosis

2008 ◽  
Vol 70 (7) ◽  
pp. 707-709 ◽  
Author(s):  
Masanobu KIMURA ◽  
Koichi IMAOKA ◽  
Michio SUZUKI ◽  
Tsuneo KAMIYAMA ◽  
Akio YAMADA
Keyword(s):  
Agglutination Test ◽  
Serological Diagnosis ◽  
Canine Brucellosis

scholarly journals Microplate Agglutination Test for Canine Brucellosis Using Recombinant Antigen-Coated Beads

2014 ◽  
Vol 2014 ◽  
pp. 1-4
Author(s):  
Yussaira Castillo ◽  
Masato Tachibana ◽  
Yui Kimura ◽  
Suk Kim ◽  
Yasuaki Ichikawa ◽  
...  
Keyword(s):  
Hypothetical Protein ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Heat Shock Protein 60 ◽  
Serum Samples ◽  
Recombinant Antigens ◽  
Latex Beads ◽  
Brucella Canis ◽  
Single Antigen ◽  
Canine Brucellosis

Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods, including agglutination and gel diffusion tests. In this study, four recombinant antigens, heat shock protein 60, rhizopine-binding protein, Cu-Zn superoxide dismutase, and hypothetical protein (Ag 4), were constructed. These antigens were coated on latex beads and their usefulness in the serological diagnosis of canine brucellosis was examined. All recombinant antigens showed specific reaction with sera from B. canis-infected dogs in Western blotting. In a microplate agglutination test, mixing sera from B. canis-infected dogs, but not sera from B. canis-free dogs, with single or multiple antigens-coated latex beads produced clear agglutination. Moreover, the antigen-coated latex beads did not show nonspecific agglutination in hemolyzed serum samples. A survey of canine serum samples conducted by the microplate agglutination test using single antigen-coated latex beads showed that this method would be useful in the serological diagnosis of canine brucellosis. Further investigations using more serum samples are required to confirm the usefulness of our method.

scholarly journals Preliminary Study of an Immunochromatography Test for Serological Diagnosis of Canine Brucellosis

2012 ◽  
Vol 47 ◽  
pp. 370-372 ◽  
Author(s):  
MM Wanke ◽  
F Cairó ◽  
M Rossano ◽  
M Laiño ◽  
PC Baldi ◽  
...  
Keyword(s):  
Serological Diagnosis ◽  
Preliminary Study ◽  
Canine Brucellosis

scholarly journals Antibodies produced by dogs successfully challenged with live Leptospira spp. did not cross-react to Brucella antigen in a commercial rapid slide agglutination test that detects antibodies to Brucella canis

2018 ◽  
Vol 31 (1) ◽  
pp. 83-85
Author(s):  
Matthew R. Krecic
Keyword(s):  
Cross Reactivity ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Serologic Tests ◽  
Experimental Infections ◽  
Negative Results ◽  
Brucella Canis ◽  
Slide Agglutination Test ◽  
Leptospira Spp ◽  
Canine Brucellosis

Brucella canis is a cause of canine infertility and abortion. Veterinarians and veterinary laboratorians screen for antibodies to B. canis with serologic tests including a rapid slide agglutination test (RSAT; D-Tec CB, Zoetis, San Diego, CA). False-positive results are possible because of cross-reactivity to antibodies to some gram-negative bacteria. Cross-reactivity has been reported between antibodies of Brucella abortus and Leptospira spp. with serologic tests for bovine brucellosis; however, this has not been documented with serologic tests for canine brucellosis, to the author’s knowledge. The RSAT was evaluated with the sera from dogs experimentally challenged with 1 of 4 serovars of Leptospira spp.: L. kirschneri serovar Grippotyphosa, or L. interrogans serovars Canicola, Icterohaemorrhagiae, or Pomona. Experimental infections were confirmed through results of microscopic agglutination testing and/or lateral flow immunochromatography testing. The sera of 32 dogs collected at day 0 and days 7, 10, and 14 yielded negative results with the RSAT. Antibodies produced through experimental infections to these 4 serovars of Leptospira spp. did not cross-react with Brucella antigen with the RSAT; therefore, cross-reactivity of anti-leptospiral antibodies may not be of concern for B. canis rapid slide agglutination testing of dogs.

scholarly journals A slide agglutination test for the exclusion and diagnosis of typhoid fever

1939 ◽  
Vol 39 (3) ◽  
pp. 294-297 ◽  
Author(s):  
F. M. Berger ◽  
G. Brecher
Keyword(s):  
Typhoid Fever ◽  
Positive Result ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Agglutination Reaction ◽  
Negative Test ◽  
Slide Agglutination ◽  
Peculiar Behaviour ◽  
Slide Agglutination Test ◽  
Agglutination Method

A sensitive antigen suspension is described for use with a simple slide agglutination method which makes possible a serological diagnosis or exclusion of typhoid fever without recourse to a laboratory. The method has been tested on 414 sera sent to our laboratory; it detected all cases with a titre of 1: 80 or more, and most of those with a titre of 1: 40. The method was further tested on 130 clinically observed cases, in which it gave satisfactory results. The S. A. method gave a positive result with 98 out of 100 sera from patients with typhoid fever, whereas the classical Widal reaction gave a positive result with 68 of them only.The intensity and rapidity of the slide agglutination reaction provide a rough measure of the titre of a serum. A quick and distinct agglutination indicates a titre of 1: 80 or more and is diagnostic of typhoid fever. A slow and indistinct result is obtained when the titre of the serum is about 1: 40. A negative test indicates with great probablity that a diagnosis of typhoid fever may be excluded.We think the method succeeds because the nature of the suspension employed and the peculiar behaviour of slide agglutinations permit the detection of O agglutinins as well as H agglutinins.

scholarly journals A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis

2016 ◽  
Vol 221 ◽  
pp. 9-13 ◽  
Author(s):  
Edward Oliveira ◽  
Juliana Wilke Saliba ◽  
Diana Oliveira ◽  
Edelberto Santos Dias ◽  
Gustavo Fontes Paz
Keyword(s):  
Visceral Leishmaniasis ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Canine Visceral Leishmaniasis ◽  
Direct Agglutination Test ◽  
Test Kit

scholarly journals One-point method for serological diagnosis of leptospirosis: a microcapsule agglutination test

1987 ◽  
Vol 99 (2) ◽  
pp. 399-405 ◽  
Author(s):  
M. Seki ◽  
T. Sato ◽  
Y. Arimitsu ◽  
T. Matuhasi ◽  
S. Kobayashi
Keyword(s):  
Early Diagnosis ◽  
Screening Test ◽  
Agglutination Test ◽  
Point Method ◽  
Serological Diagnosis ◽  
Early Stages

SUMMARYThis paper describes a simple and rapid microcapsule agglutination (MCA) test. The results obtained by this new test have been compared with those obtained by the microtitre MCA and the microscopic agglutination (MA) test. The procedures required for the new test are easier and can be performed more rapidly than those necessary for the microtitre MCA test. Furthermore, the new test is more sensitive than the MA test in the early stages of leptospirosis.This new test appears satisfactory as a screening test for the early diagnosis of leptospirosis.

scholarly journals Serological diagnosis of Toxoplasmosis by specific antibody in camels of Wasit province, Iraq

2018 ◽  
Vol 1 (29) ◽  
pp. 412-423
Author(s):  
Basim Mohammed Hanon
Keyword(s):  
Public Health ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Zoonotic Parasite

Background: toxoplasma gondii is a zoonotic parasite, more added a major public health is worldwide because have high distribution in livestock. Objectives: the main aim of this study determine the occurrence of the seroepidemiological toxoplasmosis in camels in waist province of Iraq from November 2016 to April 2017. Materials and Methods: blood samples collected of animals randomly were included six different groups of animals were diagnosed by A Latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) kits.

scholarly journals Investigations of spread of canine Brucellosis caused by Brucella canis in territory of Municipality of Pozarevac

2006 ◽  
Vol 60 (5-6) ◽  
pp. 337-344
Author(s):  
Slobodan Zivojinovic ◽  
Sonja Radojicic ◽  
Milena Zivojinovic ◽  
Jasmina Kircanski
Keyword(s):  
Positive Result ◽  
Disease Control ◽  
Reproductive Organs ◽  
Agglutination Test ◽  
Stray Dogs ◽  
Brucella Canis ◽  
Basic Source ◽  
Canine Brucellosis ◽  
Fetal Organs ◽  
Serum Agglutination

The paper examines the presence and distribution of infections caused by Brucella canis in different categories of dogs in the territory of the Municipality of Pozarevac. A total of 151 dogs were examined, and 74 blood serums originated from dogs of known owners and 77 from stray dogs. The investigations were carried out also on 40 samples of full blood of stray dogs, as well as fetal organs and reproductive organs of a serologically positive female following hysterectomy. Investigations included a clinical examination of the dogs, rapid serum agglutination, slow serum agglutination, and isolation of the cause. In all the examined dogs, the rapid agglutination test gave a positive result in 16.55% of the examined samples, the slow agglutination test 11.25%, which is an extremely high percentage in comparison with other regions of our country. Therapy using antibiotics, zoohygienic measures, castration or hysterectomy (as attempts to avoid residue and break the chain of the transfer of the infection) are conditions for out rooting the disease. Control of stray dogs is necessary, as they present the basic source of the infection. The results obtained in the course of these investigations indicate the absolute justification of including this contagious disease in the group of diseases whose reporting is compulsory.

scholarly journals Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina

2000 ◽  
Vol 7 (5) ◽  
pp. 828-831 ◽  
Author(s):  
P. Silva Paulo ◽  
A. M. Vigliocco ◽  
R. F. Ramondino ◽  
D. Marticorena ◽  
E. Bissi ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Fluorescence Polarization ◽  
Competitive Elisa ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Assays ◽  
Brucella Suis ◽  
Fluorescence Polarization Assay ◽  
Two Populations

ABSTRACT An indirect enzyme-linked immunosorbent assay (IELISA), a competitive ELISA (CELISA), and a fluorescence polarization assay (FPA) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free Canadian herds and sera from Argentina selected based on positive reactions in the buffered antigen plate agglutination test (BPAT) and the 2-mercaptoethanol (2-ME) test. In addition, sera from adult swine from which Brucella suis was isolated at least once for each farm of origin were evaluated. The IELISA, CELISA, and FPA specificity values were 99.9, 99.5, and 98.3%, respectively, and the IELISA, CELISA, and FPA sensitivity values relative to the BPAT and the 2-ME test were 98.9, 96.6, and 93.8%, respectively. Actual sensitivity was assessed by using 37 sera from individual pigs from which B. suis was cultured, and the values obtained were as follows: BPAT, 86.5%; 2-ME test, 81.1%; IELISA, 86.5%; CELISA, 78.5%; and FPA, 80.0%.

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