scholarly journals Microplate Agglutination Test for Canine Brucellosis Using Recombinant Antigen-Coated Beads

2014 ◽  
Vol 2014 ◽  
pp. 1-4
Author(s):  
Yussaira Castillo ◽  
Masato Tachibana ◽  
Yui Kimura ◽  
Suk Kim ◽  
Yasuaki Ichikawa ◽  
...  
Keyword(s):  
Hypothetical Protein ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Heat Shock Protein 60 ◽  
Serum Samples ◽  
Recombinant Antigens ◽  
Latex Beads ◽  
Brucella Canis ◽  
Single Antigen ◽  
Canine Brucellosis

Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods, including agglutination and gel diffusion tests. In this study, four recombinant antigens, heat shock protein 60, rhizopine-binding protein, Cu-Zn superoxide dismutase, and hypothetical protein (Ag 4), were constructed. These antigens were coated on latex beads and their usefulness in the serological diagnosis of canine brucellosis was examined. All recombinant antigens showed specific reaction with sera from B. canis-infected dogs in Western blotting. In a microplate agglutination test, mixing sera from B. canis-infected dogs, but not sera from B. canis-free dogs, with single or multiple antigens-coated latex beads produced clear agglutination. Moreover, the antigen-coated latex beads did not show nonspecific agglutination in hemolyzed serum samples. A survey of canine serum samples conducted by the microplate agglutination test using single antigen-coated latex beads showed that this method would be useful in the serological diagnosis of canine brucellosis. Further investigations using more serum samples are required to confirm the usefulness of our method.

scholarly journals Antibodies produced by dogs successfully challenged with live Leptospira spp. did not cross-react to Brucella antigen in a commercial rapid slide agglutination test that detects antibodies to Brucella canis

2018 ◽  
Vol 31 (1) ◽  
pp. 83-85
Author(s):  
Matthew R. Krecic
Keyword(s):  
Cross Reactivity ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Serologic Tests ◽  
Experimental Infections ◽  
Negative Results ◽  
Brucella Canis ◽  
Slide Agglutination Test ◽  
Leptospira Spp ◽  
Canine Brucellosis

Brucella canis is a cause of canine infertility and abortion. Veterinarians and veterinary laboratorians screen for antibodies to B. canis with serologic tests including a rapid slide agglutination test (RSAT; D-Tec CB, Zoetis, San Diego, CA). False-positive results are possible because of cross-reactivity to antibodies to some gram-negative bacteria. Cross-reactivity has been reported between antibodies of Brucella abortus and Leptospira spp. with serologic tests for bovine brucellosis; however, this has not been documented with serologic tests for canine brucellosis, to the author’s knowledge. The RSAT was evaluated with the sera from dogs experimentally challenged with 1 of 4 serovars of Leptospira spp.: L. kirschneri serovar Grippotyphosa, or L. interrogans serovars Canicola, Icterohaemorrhagiae, or Pomona. Experimental infections were confirmed through results of microscopic agglutination testing and/or lateral flow immunochromatography testing. The sera of 32 dogs collected at day 0 and days 7, 10, and 14 yielded negative results with the RSAT. Antibodies produced through experimental infections to these 4 serovars of Leptospira spp. did not cross-react with Brucella antigen with the RSAT; therefore, cross-reactivity of anti-leptospiral antibodies may not be of concern for B. canis rapid slide agglutination testing of dogs.

scholarly journals Investigations of spread of canine Brucellosis caused by Brucella canis in territory of Municipality of Pozarevac

2006 ◽  
Vol 60 (5-6) ◽  
pp. 337-344
Author(s):  
Slobodan Zivojinovic ◽  
Sonja Radojicic ◽  
Milena Zivojinovic ◽  
Jasmina Kircanski
Keyword(s):  
Positive Result ◽  
Disease Control ◽  
Reproductive Organs ◽  
Agglutination Test ◽  
Stray Dogs ◽  
Brucella Canis ◽  
Basic Source ◽  
Canine Brucellosis ◽  
Fetal Organs ◽  
Serum Agglutination

The paper examines the presence and distribution of infections caused by Brucella canis in different categories of dogs in the territory of the Municipality of Pozarevac. A total of 151 dogs were examined, and 74 blood serums originated from dogs of known owners and 77 from stray dogs. The investigations were carried out also on 40 samples of full blood of stray dogs, as well as fetal organs and reproductive organs of a serologically positive female following hysterectomy. Investigations included a clinical examination of the dogs, rapid serum agglutination, slow serum agglutination, and isolation of the cause. In all the examined dogs, the rapid agglutination test gave a positive result in 16.55% of the examined samples, the slow agglutination test 11.25%, which is an extremely high percentage in comparison with other regions of our country. Therapy using antibiotics, zoohygienic measures, castration or hysterectomy (as attempts to avoid residue and break the chain of the transfer of the infection) are conditions for out rooting the disease. Control of stray dogs is necessary, as they present the basic source of the infection. The results obtained in the course of these investigations indicate the absolute justification of including this contagious disease in the group of diseases whose reporting is compulsory.

scholarly journals Evaluation of a Microplate Agglutination Test (MAT) for Serological Diagnosis of Canine Brucellosis

2008 ◽  
Vol 70 (7) ◽  
pp. 707-709 ◽  
Author(s):  
Masanobu KIMURA ◽  
Koichi IMAOKA ◽  
Michio SUZUKI ◽  
Tsuneo KAMIYAMA ◽  
Akio YAMADA
Keyword(s):  
Agglutination Test ◽  
Serological Diagnosis ◽  
Canine Brucellosis

scholarly journals Epidemiological characteristics of Leptospira spp. infection in bovine herds in Mato Grosso do Sul, Brazil

2021 ◽  
Vol 58 ◽  
pp. e180127
Author(s):  
Renata Ferreira dos Santos ◽  
Danila Fernanda Rodrigues Frias ◽  
Glaucenyra Cecília Pinheiro da Silva ◽  
Talita Ribeiro Silva ◽  
Nivaldo Aparecido de Assis ◽  
...  
Keyword(s):  
Beef Cattle ◽  
High Frequency ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Serum Samples ◽  
Mato Grosso ◽  
Leptospira Spp ◽  
Cattle Herds ◽  
Epidemiological Characteristics ◽  
Mato Grosso Do Sul

The epidemiological characteristics of bovine leptospirosis in animals and herds in Mato Grosso do Sul were investigated to determine parameters such as disease frequency and the serovars reactant in beef cattle herds. A total of 4,629 beef cattle herds were examined against 33 Leptospira spp. serovars. The serum samples were submitted to the microscopic agglutination test (MAT) for the serological diagnosis of leptospirosis. The MAT results showed that 3,814 (82.39%) of the 4,629 animals evaluated were seropositive for the bacterium, with serological reactions mainly to serogroup Sejroe, serovar Wolffi (36.49%). The observed high frequency of reactive animals demonstrates the relevance of the infection. Therefore, general and specific measures should be implemented to contain and/or prevent infection of the animals in the studied region.

Investigation of The Presence of Brucella canis in Dogs in Western Part of Turkey

2020 ◽  
Author(s):  
U. Parin ◽  
E. Goksel ◽  
H. T.Y. Dolgun ◽  
S. Kirkan ◽  
S. Turkyilmaz ◽  
...  
Keyword(s):  
Close Contact ◽  
Testicular Atrophy ◽  
Molecular Testing ◽  
Serum Samples ◽  
Stray Dogs ◽  
Molecular Tests ◽  
Specific Pcr ◽  
Brucella Canis ◽  
Species Specific ◽  
Canine Brucellosis

Background: Brucellosis is a zoonotic infection that usually settles in the genital organs, causing chronic, infectious and necrotic inflammatory disorders characterised by abortion and infertility mastitis in females and orchitis, epididymitis and testicular atrophy in male animals. Brucella canis causes Brucella infections in dogs. Less concern has showed to B. canis in Turkey, although dogs usually live in close contact with their owners and there is an increasing incidence of breeding kennels and stray dogs with poor state of health. The scope of this study is to determine the regional prevalance of canine brucellosis caused by Brucella canis using a 2-mercaptaethanol rapid slide agglutination test and species-specific PCR method in the western part of Turkey. Methods: A total of 200 blood samples (80 from Aydýn Province, 60 from Ýzmir Province and 60 from Muðla Province) were collected between March 2019 and February 2020. Serological analysis was performed via mercaptoethanol microagglutination test. Brucella canis-specific PCR procedures were used to detect brucellosis at the molecular level. Result: Twelve (6%) of the serum samples were positive for 2-ME RSAT. B. canis positivity was detected in 10 (5%) out of 200 samples via PCR test. Twelve positive samples were found via 2-ME RSAT and 10 positive samples were found via molecular testing. B. canis positivity was 7.5% in Aydýn and 6.6% in Muðla, as revealed via molecular tests in pet dogs in contact with stray dogs. The presence of B. canis was not observed in the dogs examined from Ýzmir Province, which were not in contact with strays.

Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis

2011 ◽  
Vol 90 (3) ◽  
pp. 425-431 ◽  
Author(s):  
Maria Zoraida Daltro de Oliveira ◽  
Vera Vale ◽  
Lara Keid ◽  
Songeli Menezes Freire ◽  
Roberto Meyer ◽  
...  
Keyword(s):  
Serological Diagnosis ◽  
Elisa Method ◽  
Brucella Canis ◽  
Canine Brucellosis

scholarly journals Serological Diagnosis of Human Cysticercosis by Use of Recombinant Antigens from Taenia solium Cysticerci

1999 ◽  
Vol 6 (4) ◽  
pp. 479-482 ◽  
Author(s):  
Kerstin Hubert ◽  
Abel Andriantsimahavandy ◽  
Alain Michault ◽  
Matthias Frosch ◽  
Fritz A. Mühlschlegel
Keyword(s):  
Immunosorbent Assay ◽  
Fusion Proteins ◽  
Taenia Solium ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cdna Expression Library ◽  
Serum Samples ◽  
Expression Library ◽  
Recombinant Antigens ◽  
Cdna Expression

ABSTRACT A Taenia solium metacestode cDNA expression library in the lambda ZAPII vector was screened with pooled sera from patients with neurocysticercosis. Sixty primary clones were identified and shown to belong to two classes. The clones NC-3 and NC-9 did not reveal any significant homologies to sequences deposited in the databases and were further characterized. Both recombinant antigens were expressed as glutathione S-transferase fusion proteins and applied for serological diagnosis of human cysticercosis. An enzyme-linked immunosorbent assay was established and evaluated with 27 serum samples of La Réunion and Madagascar patients with cysticercosis. Diagnosis in these patients was established with radiological and serological procedures. For antigen NC-3 a sensitivity of 96.3% and a specificity of 91.5% for the serodiagnosis were achieved. In contrast, the sensitivity of antigen NC-9 was only 33.3%.

scholarly journals COMPARATIVE EVALUATION OF COMMERCIAL SERODIAGNOSTIC TESTS FOR THE SEROPREVALENCE STUDY OF BRUCELLOSIS IN STRAY DOGS IN BANGLADESH

2012 ◽  
Vol 9 (1) ◽  
pp. 79-83 ◽  
Author(s):  
BC Talukder ◽  
MA Samad ◽  
AKMA Rahman
Keyword(s):  
Prevalence Rate ◽  
Age Groups ◽  
Standard Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Stray Dogs ◽  
High Prevalence ◽  
Canine Brucellosis ◽  
A Prospective Study

A prospective study of Brucella sero-prevalence was conducted in 30 stray dogs in Mymensingh Municipal Corporation area of Bangladesh during the period from January to December 2009. All the sera were screened primarily by Rose Bengal Plate Test (RBPT). Positive, doubtful and negative serum samples were further confirmed with Slow Agglutination Test (SAT), Standard Tube Agglutination Test (STAT) and indirect Enzyme Linked Immunosorbent Assay (ELISA) and all the SAT, STAT and ELISA negative samples were confirmed as negative. The sensitivity of RBPT, SAT and STAT was found to be of 100%, 66.67% and 66.67% with considering ELISA as a standard test while specificity was found to be of 96.29%, 100% and 100% in stray dogs. This indicates that the SAT and STAT are found more specific than RBPT. Evaluation of comparison of sero-prevalence rates of canine brucellosis with the four sero-tests advocated to use ELISA for diagnosis of canine brucellosis. The overall sero-prevalence of canine brucellosis was recorded as 13.33%, 6.67%, 6.67% and 10.0% with RBPT, SAT, STAT and ELISA, respectively. Sero-results have analyzed on the basis of two age groups (up to 6 months and 7 to 36 months) and sex (male and female). Significantly (p<0.01) higher sero-prevalence rate of canine brucellosis was recorded in stray dogs aged between 7 to 36 months (14.81%, 7.40%, 7.40% and 11.11%) in comparison to aged group up to 6 months ( 0%, 0%, 0% and 0% ) with RBPT, SAT, STAT and ELISA, respectively. The sero-prevalence rate of canine brucellosis was found significantly (p <0.01) higher in female dogs (15.78%, 10.52%, 10.52% and 15.78%) in comparison to male (9.09%, 0%, 0% and 0%) with RBPT, SAT, STAT and ELISA, respectively. It appears from this study that brucellosis is an endemic disease and measures are needed to reduce this high prevalence of canine brucellosis in Bangladesh.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11217 Bangl. J. Vet. Med. (2011). 9(1): 79-83 

scholarly journals Preliminary Study of an Immunochromatography Test for Serological Diagnosis of Canine Brucellosis

2012 ◽  
Vol 47 ◽  
pp. 370-372 ◽  
Author(s):  
MM Wanke ◽  
F Cairó ◽  
M Rossano ◽  
M Laiño ◽  
PC Baldi ◽  
...  
Keyword(s):  
Serological Diagnosis ◽  
Preliminary Study ◽  
Canine Brucellosis
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