scholarly journals Development of an ELISA Based on the Baculovirus-Expressed Capsid Protein of Porcine Circovirus Type 2 as Antigen

2004 ◽  
Vol 66 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Changming LIU ◽  
Takeshi IHARA ◽  
Tetsuo NUNOYA ◽  
Susumu UEDA
Keyword(s):  
Capsid Protein ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

Comparing the efficiency of different Escherichia coli strains in producing recombinant capsid protein of porcine circovirus type 2

2020 ◽  
Vol 52 ◽  
pp. 101556 ◽  
Author(s):  
Vishal Rai ◽  
Vikramaditya Upmanyu ◽  
Gulam Mohd ◽  
Ravi Kumar ◽  
Sanganagouda Koppad ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Capsid Protein ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

scholarly journals Efficient Production of Porcine Circovirus Type 2 Capsid Protein using Baculovirus

2012 ◽  
Vol 24 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Jun-Beom Lee ◽  
Sung-Min Bae ◽  
Hee-Jung Kim ◽  
Won-Woo Lee ◽  
Won-Il Heo ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Efficient Production

scholarly journals Evaluation of a Virus-like Nanoparticle Porcine Circovirus Type-2 (PCV2) Capsid Protein Fused with the Pig Immunoglobulin Fc Fragment as a Novel Vaccine Candidate against PCV2 in Mice

Vaccines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1128
Author(s):  
Qingping Luo ◽  
Waqas Ahmed ◽  
Yichen Dai ◽  
Ali Mohsin ◽  
Haifeng Hang ◽  
...  
Keyword(s):  
Immune Responses ◽  
Fusion Protein ◽  
Capsid Protein ◽  
Ex Vivo ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Cell Reactivity ◽  
Fc Fragment

Porcine circovirus Type 2 (PCV2) is a primary etiological pathogen of post-weaning multi-systemic wasting syndrome (PMWS). The capsid protein of PCV2 is the crucial immunogenic protein which can induce antibody generation and immune responses. However, there is still a lack of efficient PCV2 vaccines with high immunogenicity. In the current study, we developed a novel engineered PCV2 capsid (∆1-41aa)-pFc fusion protein (PCFP), which comprised a truncated capsid protein of PCV2 and a porcine IgG Fc fragment, fused to the capsid protein of PCV2 at the C-terminus. We found that this novel fusion protein could auto-assemble into virus-like nanoparticles with an estimated mean diameter of 22.6 nm, characterized by transmission electron microscopy. Immunization of BALB/c mice with this fusion protein significantly increased the production levels of anti-PCV2-capsid protein antibody in serum. Besides, the virus-like nanoparticles, PCFP was demonstrated to induce efficient cellular immune responses in mice, as evident by the high specific T cell reactivity to the PCFP fusion protein and the high production of the immune cytokines IFN-γ and IL-10 in an ex vivo re-stimulation system. Collectively, these findings demonstrate that the PCV2 truncated capsid subunit Fc-fusion protein can induce both cellular and humoral immune responses, and it displays great application potential.

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