scholarly journals Generation of Multinucleated Giant Cells In Vitro from Bovine Monocytes and Macrophages

2004 ◽  
Vol 66 (9) ◽  
pp. 1065-1069 ◽  
Author(s):  
Kazuhiro YOSHIHARA ◽  
Reiko NAGATA ◽  
Yoshihiro MUNETA ◽  
Shigeki INUMARU ◽  
Yuichi YOKOMIZO ◽  
...  
Keyword(s):  
Giant Cells ◽  
Multinucleated Giant Cells ◽  
Monocytes And Macrophages

scholarly journals TRANSFORMATION OF MONOCYTES IN TISSUE CULTURE INTO MACROPHAGES, EPITHELIOID CELLS, AND MULTINUCLEATED GIANT CELLS

1966 ◽  
Vol 28 (2) ◽  
pp. 303-332 ◽  
Author(s):  
Jerry S. Sutton ◽  
Leon Weiss
Keyword(s):  
Golgi Apparatus ◽  
Plasma Membranes ◽  
Giant Cells ◽  
Progressive Increase ◽  
Multinucleated Giant Cells ◽  
Epithelioid Cells ◽  
Cytoplasmic Filaments ◽  
Monocytes And Macrophages ◽  
Sequential Transformation

The sequential transformation of chicken monocytes into macrophages, epithelioid cells, and multinucleated giant cells in vitro was studied by electron microscopy after fixation and embedment in situ. The following changes occur. In the nucleus, margination of chromatin, evident in monocytes, decreases in later forms. Nucleoli become more complex and nuclear pores increase in number. In cytoplasm, a progressive increase in volume of the ectoplasm and endoplasm occurs in culture. Lysosomes increase in number and size prior to phagocytosis. During phagocytosis (most active from 1 to 3 days of culture) lysosome depletion occurs. Lysosomes are present in greatest number and show maximal structural variation in the epithelioid and young giant cells. Aging giant cells lose lysosomes. All stages possess variably large quantities of rough-surfaced endoplasmic reticulum and free ribosomes. The Golgi apparatus, small in monocytes, increases in size and complexity. Massive accumulations of lysosomes within the Golgi apparatus of macrophages and epithelioid cells suggest that lysosomes originate there. In giant cells, multiple Golgi regions occur, often ringing the nuclei. Monocytes and macrophages have few mitochondria. Mitochondria of epithelioid cells are larger, more numerous, and may have discontinuous outer membranes. Mitochondria are most numerous in giant cells where they increase with age and become polymorphous. Cytoplasmic filaments are approximately 50 to 60 A in diameter and of indeterminate length. They occur both singly and in bundles which touch cytoplasmic vesicles and mitochondria. Few filaments occur in monocytes and macrophages. A large increase in the number of filaments occurs in epithelioid cells, where filaments (90 to 100 A) surround the cytocentrum as a distinctive annular bundle often branching into the cytoplasm. The greatest concentration of filaments occurs in aged giant cells. Pseudopodia are always present. They are short and filiform in monocytes and giant cells, and broad, with abundant micropinocytotic vesicles, in macrophages and epithelioid cells. At every stage, the cell membrane contains dense cuplike structures. These may represent the membranous residue of lysosomes which have discharged to the outside, analogous to merocrine secretion. Contiguous epithelioid cells display elaborate cytoplasmic interdigitation. In places, the plasma membranes break down and epithelioid cells fuse to form giant cells.

scholarly journals Mesenchymal stromal cells' secretome enhances osteoclastogenesis but reduces multinucleated giant cells formation in vitro

Bone Reports ◽  
2020 ◽  
Vol 13 ◽  
pp. 100371
Author(s):  
Paul Humbert ◽  
Julien De Lima ◽  
Meadhbh Á. Brennan ◽  
Frédéric Blanchard ◽  
Pierre Layrolle
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Giant Cells ◽  
Multinucleated Giant Cells

Osseoinduction Evaluation of Hydroxyapatite and Zinc Containing Hydroxyapatite Granules in Rabbits

2011 ◽  
Vol 493-494 ◽  
pp. 252-257 ◽  
Author(s):  
L. Nascimento ◽  
M. Medeiros ◽  
J. Calasans-Maia ◽  
A. Alves ◽  
Antonella M. Rossi ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Bone Matrix ◽  
Fibrous Tissue ◽  
Particle Diameter ◽  
Inflammatory Cells ◽  
Giant Cells ◽  
Multinucleated Giant Cells ◽  
Ethics Commission

This study investigated the osteoinductive potential of granules of stoichiometric hydroxyapatite (HA) and 0.5% zinc containing hydroxyapatite (ZnHA) in intramuscular (IM) site of rabbit’s abdomen. The biomaterials were both used in granular form, with 75% porosity and particle diameter between 450 and 500μm, sintered at 1100°C. Both materials performed adequately on a multiparametric in vitro cytocompatibility assay, indicating their suitability for in vivo testing. After approval by the Ethics Commission on Teaching and Research in Animals, fifteen rabbits were submitted to general anesthesia, incision and tissue dilatation, and a small site was created for HA (right incision) and ZnHA (left incision) intramuscular implantation. The animals were killed after 2, 4 and 12 weeks for biomaterials and surrounding tissues removal. Histological analysis after 2 weeks revealed the presence of granulation tissue surrounding biomaterials with multinucleated giant cells and no newly formed bone for both materials. After 4 weeks there was fibrous tissue involving the material and few inflammatory cells. Following 12 weeks it was observed the presence of connective tissue surrounding the biomaterial, cellularized enough for the two experimental groups, but it was not observed the presence of bone matrix associated with the biomaterials. We conclude that both biomaterials are cytocompatible and did not present the property of osseoinduction after 12 weeks of implantation.

scholarly journals Formation of Multinucleated Giant Cells In Vitro Is Dependent on the Stage of Monocyte to Macrophage Maturation

Blood ◽  
1997 ◽  
Vol 89 (2) ◽  
pp. 662-671 ◽  
Author(s):  
Johannes Möst ◽  
Ludwig Spötl ◽  
Gertraud Mayr ◽  
Annette Gasser ◽  
Alessandra Sarti ◽  
...  
Keyword(s):  
Human Peripheral Blood ◽  
Giant Cells ◽  
Multinucleated Giant Cells ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Inflammatory Reactions ◽  
Serum Free Conditions ◽  
Two Populations ◽  
Stimulation Of

Abstract Multinucleated giant cells (MGC) are a common feature of granulomas that develop during various inflammatory reactions. MGC originate from fusion of monocytes or macrophages, but the exact mechanism of their generation is still unclear. In the present study, we investigated the influence of monocyte to macrophage maturation on the ability of human monocytes/macrophages to fuse with each other. MGC were generated in vitro by stimulation of human peripheral blood monocytes with cytokine containing supernatants. With freshly isolated monocytes, fusion rates of up to 90% were obtained. When monocyte to macrophage maturation was induced by culturing the cells in human serum, fusion rates gradually decreased with advancing time of the preceding culture (corresponding to the stage of differentiation) and almost no MGC formation could be obtained with 8-day-old macrophages. In contrast, fusion rates did not decrease when monocytes had been cultured under serum free conditions before stimulation. When freshly isolated monocytes were added to 1-week cultured macrophages, which had been membrane-labeled with a fluorochrome, fusion between the two populations could be induced. Because the ability for intracellular killing of certain pathogens is reduced in macrophages, fusion with monocytes (newly arriving at the site of inflammation) may represent an attempt to restore this capacity.

Effects of Lectin (ScLL) on osteoclast-like multinucleated giant cells’ maturation-A preliminary in vitro study

2018 ◽  
Vol 34 (5) ◽  
pp. 329-335
Author(s):  
Manuella V. P. Reis ◽  
Gabriela L. de Souza ◽  
Camilla C. G. Moura ◽  
Marcus V. da Silva ◽  
Maria A. Souza ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Giant Cells ◽  
Vitro Study ◽  
Multinucleated Giant Cells

In vitro and in vivo assessment of CaP materials for bone regenerative therapy. The role of multinucleated giant cells/osteoclasts in bone regeneration

2019 ◽  
Vol 108 (1) ◽  
pp. 282-297 ◽  
Author(s):  
Ana Carolina Cestari Bighetti ◽  
Tania Mary Cestari ◽  
Paula Sanches Santos ◽  
Ricardo Vinicius Nunes Arantes ◽  
Suelen Paini ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Giant Cells ◽  
Regenerative Therapy ◽  
Multinucleated Giant Cells ◽  
In Vivo Assessment

scholarly journals Apoptotic mesenchymal stromal cells support osteoclastogenesis while inhibiting multinucleated giant cells formation in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Paul Humbert ◽  
Meadhbh Á. Brennan ◽  
Julien De Lima ◽  
Régis Brion ◽  
Annie Adrait ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Quantitative Proteomics ◽  
Giant Cells ◽  
Multinucleated Giant Cells ◽  
Short Term ◽  
Before And After ◽  
Induced Apoptosis

AbstractIn bone regeneration induced by the combination of mesenchymal stromal cells (MSCs) and calcium-phosphate (CaP) materials, osteoclasts emerge as a pivotal cell linking inflammation and bone formation. Favorable outcomes are observed despite short-term engraftments of implanted MSCs, highlighting their major paracrine function and the possible implication of cell death in modulating their secretions. In this work, we focused on the communication from MSCs towards osteoclasts-like cells in vitro. MSCs seeded on a CaP biomaterial or undergoing induced apoptosis produced a conditioned media favoring the development of osteoclasts from human CD14+ monocytes. On the contrary, MSCs’ apoptotic secretion inhibited the development of inflammatory multinucleated giant cells formed after IL-4 stimulation. Components of MSCs’ secretome before and after apoptotic stress were compared using mass spectrometry-based quantitative proteomics and a complementary immunoassay for major cytokines. CXCR-1 and CXCR-2 ligands, primarily IL-8/CXCL-8 but also the growth-regulated proteins CXCL-1, -2 or -3, were suggested as the major players of MSCs’ pro-osteoclastic effect. These findings support the hypothesis that osteoclasts are key players in bone regeneration and suggest that apoptosis plays an important role in MSCs’ effectiveness.

In Vitro Formation of Macrophage- Epithelioid Cells and Multinucleated Giant Cells by 1?,25-Dihydroxyvitamin D3from Human Circulating Monocytes

1986 ◽  
Vol 465 (1 Tenth Interna) ◽  
pp. 211-220 ◽  
Author(s):  
MASATAKA OHTA ◽  
TETSURO OKABE ◽  
KEIYA OZAWA ◽  
AKIO URABE ◽  
FUMIMARO TAKAKU
Keyword(s):  
Giant Cells ◽  
Multinucleated Giant Cells ◽  
Epithelioid Cells

Fungicidal activity of human monocyte-derived multinucleated giant cells induced in vitro by Paracoccidioides brasiliensis antigen

2008 ◽  
Vol 166 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Magda Paula Pereira do Nascimento ◽  
Angela Maria Victoriano de Campos Soares ◽  
Luciane Alarcão Dias-Melicio ◽  
Maria Rita Parise-Fortes ◽  
Rosana Aparecida Rodrigues Martins ◽  
...  
Keyword(s):  
Fungicidal Activity ◽  
Paracoccidioides Brasiliensis ◽  
Human Monocyte ◽  
Giant Cells ◽  
Multinucleated Giant Cells
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