scholarly journals A Reduction in the Amount and Anti-Contractile Effect of Periadventitial Mesenteric Adipose Tissue Precedes Hypertension Development in Spontaneously Hypertensive Rats

2008 ◽  
Vol 31 (7) ◽  
pp. 1415-1423 ◽  
Author(s):  
Beatriz GÁLVEZ-PRIETO ◽  
Galyna DUBROVSKA ◽  
M. Victoria CANO ◽  
Mercedes DELGADO ◽  
Isabel ARANGUEZ ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Mesenteric Adipose Tissue ◽  
Contractile Effect ◽  
Hypertension Development

Abstract 11003: Hypothalamic Activated Microglia With Morphological Changes Accelerate Blood Pressure Elevation During the Hypertension Development Phase in Stroke-prone Spontaneously Hypertensive Rats

Circulation ◽  
2015 ◽  
Vol 132 (suppl_3) ◽  
Author(s):  
Ko Takesue ◽  
Takuya Kishi ◽  
Yoshitaka Hirooka
Keyword(s):  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Development Phase ◽  
Hypertensive Rats ◽  
Blood Pressure Elevation ◽  
Spontaneously Hypertensive ◽  
Activated Microglia ◽  
Wky Rats ◽  
Pressure Elevation ◽  
Hypertension Development

Introduction: A recent paradigm shift in cardiovascular pathophysiology is the impact of inflammation on hypertension. Inflammation within the paraventricular nucleus of the hypothalamus (PVN) is an important pathology of sympathetic hyperactivity, and is mainly mediated by innate immune cells, microglia. Activated microglia with alteration of their morphology produce inflammatory cytokines. Previous reports demonstrated that microglia within the PVN have activated morphology in angiotensin II-induced hypertensive rats and spontaneously hypertensive rats compared with normotensive control Sprague-Dawley rats or Wistar-Kyoto (WKY) rats. However, the role of activated microglia in the PVN in blood pressure elevation associated with sympathetic hyperactivity remains unknown. In the present study, we determined whether inhibition of microglial activation within the PVN attenuates the blood pressure elevation in genetically hypertensive rats. Methods and Results: We evaluated the activation of PVN microglia, identified by microglia specific ionized calcium-binding adaptor molecule 1 immunoreactivity, by measuring the roundness and the perimeter of microglia at 6 weeks of age, early hypertension development phase in stroke-prone spontaneously hypertensive rats (SHRSP) and compared with them in age-matched normotensive WKY rats. At 6 weeks of age, increased roundness and shortening of perimeter of microglia, indicating activated microglia, were observed in SHRSP compared with those in WKY rats. Then, we performed intracerebroventricular (ICV) administration of minocycline (5 μg/h) to deactivate microglia at 6 weeks of age for 4 weeks. ICV administration of minocycline significantly attenuated systolic blood pressure elevation in SHRSP over 4 weeks (at the end of experiments; 203.2±2.2 mm Hg vs. 215.9±2.7 mm Hg, n=8-9, P<0.05), but not in WKY rats. At 10 weeks of age, morphological analysis revealed that ICV minocycline significantly decreased the roundness and increased the perimeter of microglia, indicating deactivation of microglia, within the PVN in SHRSP. Conclusions: Hypothalamic activated microglia with morphologic changes accelerate blood pressure elevation during the hypertension development phase in SHRSP.

scholarly journals Perivascular Adipose Tissue and Mesenteric Vascular Function in Spontaneously Hypertensive Rats

2006 ◽  
Vol 26 (6) ◽  
pp. 1297-1302 ◽  
Author(s):  
Beatriz Gálvez ◽  
Javier de Castro ◽  
Diana Herold ◽  
Galyna Dubrovska ◽  
Silvia Arribas ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Vascular Function ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Perivascular Adipose Tissue ◽  
Spontaneously Hypertensive

scholarly journals Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension

2021 ◽  
Vol 22 (21) ◽  
pp. 11812
Author(s):  
Atsunori Yamamoto ◽  
Kosuke Otani ◽  
Muneyoshi Okada ◽  
Hideyuki Yamawaki
Keyword(s):  
Adipose Tissue ◽  
Protein Expression ◽  
Western Blotting ◽  
Spontaneously Hypertensive Rats ◽  
Biologically Active ◽  
Systemic Hypertension ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Peripheral Protein ◽  
Wistar Kyoto

Adipocytokine chemerin is a biologically active molecule secreted from adipose tissue. Chemerin elicits a variety of functions via chemokine-like receptor 1 (CMKLR1). The cardiovascular center in brain that regulates blood pressure (BP) is involved in pathophysiology of systemic hypertension. Thus, we explored the roles of brain chemerin/CMKLR1 on regulation of BP in spontaneously hypertensive rats (SHR). For this aim, we examined effects of intracerebroventricular (i.c.v.) injection of CMKLR1 small interfering (si)RNA on both systemic BP as measured by tail cuff system and protein expression in paraventricular nucleus (PVN) of SHR as determined by Western blotting. We also examined both central and peripheral protein expression of chemerin by Western blotting. Systolic BP of SHR but not normotensive Wistar Kyoto rats (WKY) was decreased by CMKLR1 siRNA. The decrease of BP by CMKLR1 siRNA persisted for 3 days. Protein expression of CMKLR1 in PVN of SHR tended to be increased compared with WKY, which was suppressed by CMKLR1 siRNA. Protein expression of chemerin in brain, peripheral plasma, and adipose tissue was not different between WKY and SHR. In summary, we for the first time revealed that the increased protein expression of CMKLR1 in PVN is at least partly responsible for systemic hypertension in SHR.

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