PRONUCLEAR SYNCHRONIZATION AND NUCLEAR MORPHOLOGY OF MATURE AND IN VITRO MATURED OOCYTES IN THE RAT: AN ULTRASTRUCTURAL STUDY

2005 ◽  
Vol 41 (8) ◽  
pp. 272 ◽  
Author(s):  
M. CINCIK ◽  
B. BAYKAL ◽  
S. ZETEROGLU ◽  
G. ONALAN ◽  
S. T. CEYHAN ◽  
...  
Keyword(s):  
Ultrastructural Study ◽  
Nuclear Morphology

Furazolidone is a selective in vitro candidate against Leishmania (L.) chagasi: an ultrastructural study

2010 ◽  
Vol 106 (6) ◽  
pp. 1465-1469 ◽  
Author(s):  
Juliana Quero Reimão ◽  
Noemi Nosomi Taniwaki ◽  
André Gustavo Tempone
Keyword(s):  
Ultrastructural Study

Dipetalonema viteae: ultrastructural study on the in vitro interaction between rat macrophages and microfilariae in the presence of IgE antibody

Parasitology ◽  
1981 ◽  
Vol 82 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. A. Ouaissi ◽  
A. Haque ◽  
A. Capron
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Peritoneal Macrophages ◽  
Immune Serum ◽  
Sequence Of Events ◽  
Transmission Electron ◽  
Dipetalonema Viteae ◽  
In Vitro Interaction

SUMMARYThe in vitro interaction between rat peritoneal macrophages and Dipetalonema viteae microfilariae in the presence of amicrofilaraemic rat immune serum was studied by transmission electron microscopy. The probable sequence of events leading to the killing of D. viteae microfilaria by macrophages is as follows. (a) Rat peritoneal macrophages in the presence of amicrofilaraemic rat immune serum adhere to the parasite surface, (b) the macrophages extend their pseudopodia around the parasite, (c) the ‘lysosome-like’ granules discharge their contents on to the parasite surface, (d) the lytic activity of these products begins at the parasite surface and (e) subsequent breaking of the microfilarial cuticle occurs, exposing the parasite intracellular material.

scholarly journals Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia

2003 ◽  
Vol 98 (7) ◽  
pp. 905-908 ◽  
Author(s):  
Wolfan Araque ◽  
Emilia E Barrios ◽  
Pedro Rodríguez ◽  
Víctor S Delgado ◽  
Héctor J Finol
Keyword(s):  
Schistosoma Mansoni ◽  
Ultrastructural Study ◽  
Biomphalaria Glabrata ◽  
In Vitro Interaction

Cell surface modifications in the epithelium of rat ventral prostate during adaptation to in vitro conditions: An ultrastructural study

In Vitro ◽  
1984 ◽  
Vol 20 (3) ◽  
pp. 216-228 ◽  
Author(s):  
Frederick B. Merk ◽  
Paul W. L. Kwan ◽  
Stanley Spilman ◽  
Louis Terracio ◽  
William H. J. Douglas
Keyword(s):  
Cell Surface ◽  
Ultrastructural Study ◽  
Surface Modifications ◽  
Ventral Prostate ◽  
Rat Ventral Prostate

scholarly journals Ultrastructural study of the pineal germinoma in vivo and in vitro

Cancer ◽  
1976 ◽  
Vol 37 (1) ◽  
pp. 327-337 ◽  
Author(s):  
William R. Markesbery ◽  
William H. Brooks ◽  
Larry Milsow ◽  
Richard H. Mortara
Keyword(s):  
Ultrastructural Study ◽  
Pineal Germinoma

An ultrastructural study of phagocytosis in bone by osteoblastic cells from fetal mouse calvaria in vitro

1986 ◽  
Vol 31 (10) ◽  
pp. 703-706 ◽  
Author(s):  
T. Takahashi ◽  
N. Kurihara ◽  
K. Takahashi ◽  
M. Kumegawa
Keyword(s):  
Ultrastructural Study ◽  
Osteoblastic Cells ◽  
Fetal Mouse ◽  
Mouse Calvaria

Osteogenesis in vitro: An ultrastructural study

Bone ◽  
1985 ◽  
Vol 6 (6) ◽  
pp. 484-485
Author(s):  
H.C. Tenenbaum ◽  
D. Holmyard ◽  
K. Palangio ◽  
K.P.H. Pritzker
Keyword(s):  
Ultrastructural Study ◽  
Osteogenesis In Vitro

Nuclear morphology, diameter and meiotic competence of buffalo oocytes relative to follicle size

2003 ◽  
Vol 15 (4) ◽  
pp. 223 ◽  
Author(s):  
Muhammad Rizwan Yousaf ◽  
Kazim Raza Chohan
Keyword(s):  
Germinal Vesicle ◽  
Cumulus Cells ◽  
Nuclear Morphology ◽  
Vitro Fertilization ◽  
Size Dependent ◽  
Size Groups ◽  
Follicle Size ◽  
Fetal Bovine ◽  
Meiotic Competence

The nuclear morphology, diameter and in vitro meiotic competence of buffalo oocytes was compared relative to follicle size. Cumulus–oocyte complexes (COCs) were collected from 1–<2, 2–<3, 3–<4, 4–<6 and 6–<8 mm follicles from abattoir ovaries. Cumulus cells were removed using 3 mg mL−1 hyaluronidase in saline and repeated pipetting. Denuded oocytes were measured, fixed in 3% glutaraldehyde, stained with 4,6-diamidoino-2-phenylindole and evaluated for nuclear morphology, namely the stage of germinal vesicle (GV) development before in vitro maturation (IVM). The COCs from >2-mm follicles were matured in vitro in their respective size groups for 24 h in Medium 199 supplemented with 10 μg mL−1 follicle-stimulating hormone, 10 μg mL−1 luteinizing hormone, 1.5 μg mL−1 oestradiol, 75 μg mL−1 streptomycin, 100 IU mL−1 penicillin, 10 mM HEPES and 10% fetal bovine serum. Matured oocytes were fixed, stained and evaluated for GV status and meiotic development. The number of oocytes collected from follicles 1–<8 mm in diameter averaged 1.82 per ovary. Oocytes from follicles 1–<2 mm (107.7 ± 1.6 μm), 2–<3 mm (108 ± 1.1 μm) and 3–<4 mm (114.6 ± 1.3 μm) in diameter were smaller in diameter (P < 0.05) than oocytes from follicles 4–<6 mm (124.4 ± 1.3 μm) and 6–<8 mm (131.9 ± 1.4 μm) in diameter. A majority of oocytes (P < 0.05) from <4-mm follicles was at the initial stages of GV development (GV-I, II and III), whereas oocytes from 4–<6- and 6–<8-mm follicles were at the final stages of GV-IV (35.0 and 21.6% respectively) and GV-V (49.1 and 67.5% respectively). Poor IVM rates of 32.0% and 32.7% to metaphase (M)-II were observed for oocytes isolated from 2–<3- and 3–<4-mm follicles, respectively, whereas significantly (P < 0.05) more oocytes from 4–<6- and 6–<8-mm follicles reached M-II (67.1% and 79.1% respectively). In conclusion, buffalo oocytes displayed a size-dependent ability to undergo meiotic maturation and we suggest that oocytes from >4-mm follicles should be considered in buffalo in vitro fertilization systems for better results.

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