Effects of Platinum-Thymine on Mitosis of Sarcoma-180 Ascites Cells in vitro: Ultrastructural Study

Chemotherapy ◽  
1988 ◽  
Vol 34 (2) ◽  
pp. 134-141 ◽  
Author(s):  
G. Ofosu ◽  
R. Bowden
Keyword(s):  
Ultrastructural Study ◽  
Sarcoma 180

Cytological changes induced by platinum-thymine in sarcoma-180 cells: Electron microscopy study

1990 ◽  
Vol 48 (3) ◽  
pp. 290-291
Author(s):  
Gustav Ofosu
Keyword(s):  
Mammalian Cells ◽  
Antitumor Agent ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Limiting Factor ◽  
Sarcoma 180 ◽  
Electron Microscopic ◽  
Cytological Changes

Platinum-thymine has been found to be a potent antitumor agent, which is quite soluble in water, and lack nephrotoxicity as the dose-limiting factor. The drug has been shown to interact with DNA and inhibits DNA, RNA and protein synthesis in mammalian cells in vitro. This investigation was undertaken to elucidate the cytotoxic effects of piatinum-thymine on sarcoma-180 cells in vitro ultrastructurally, Sarcoma-180 tumor bearing mice were treated with intraperitoneal injection of platinum-thymine 40mg/kg. A concentration of 60μg/ml dose of platinum-thymine was used in in vitro experiments. Treatments were at varying time intervals of 3, 7 and 21 days for in vivo experiments, and 30, 60 and 120 min., 6, 12, and 24th in vitro. Controls were not treated with platinum-thymine.Electron microscopic analyses of the treated cells in vivo and in vitro showed drastic cytotoxic effect.

Furazolidone is a selective in vitro candidate against Leishmania (L.) chagasi: an ultrastructural study

2010 ◽  
Vol 106 (6) ◽  
pp. 1465-1469 ◽  
Author(s):  
Juliana Quero Reimão ◽  
Noemi Nosomi Taniwaki ◽  
André Gustavo Tempone
Keyword(s):  
Ultrastructural Study

Dipetalonema viteae: ultrastructural study on the in vitro interaction between rat macrophages and microfilariae in the presence of IgE antibody

Parasitology ◽  
1981 ◽  
Vol 82 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. A. Ouaissi ◽  
A. Haque ◽  
A. Capron
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Peritoneal Macrophages ◽  
Immune Serum ◽  
Sequence Of Events ◽  
Transmission Electron ◽  
Dipetalonema Viteae ◽  
In Vitro Interaction

SUMMARYThe in vitro interaction between rat peritoneal macrophages and Dipetalonema viteae microfilariae in the presence of amicrofilaraemic rat immune serum was studied by transmission electron microscopy. The probable sequence of events leading to the killing of D. viteae microfilaria by macrophages is as follows. (a) Rat peritoneal macrophages in the presence of amicrofilaraemic rat immune serum adhere to the parasite surface, (b) the macrophages extend their pseudopodia around the parasite, (c) the ‘lysosome-like’ granules discharge their contents on to the parasite surface, (d) the lytic activity of these products begins at the parasite surface and (e) subsequent breaking of the microfilarial cuticle occurs, exposing the parasite intracellular material.

In Vivo and in Vitro Proliferation Kinetics of Sarcoma 180 in Solid Form

1972 ◽  
Vol 58 (5) ◽  
pp. 335-339 ◽  
Author(s):  
Rosella Silvestrini ◽  
Ornella Sanfilippo ◽  
Luigi Lenaz
Keyword(s):  
Double Labelling ◽  
Pulse Labelling ◽  
Sarcoma 180 ◽  
In Vitro Proliferation ◽  
Proliferation Kinetics ◽  
Human Solid Tumors ◽  
A Cell ◽  
Kinetics Of

In order to obtain data for setting up a rapid and relatively inexpensive method for studying the proliferation kinetics of human solid tumors, we have determined the kinetic parameters of an experimental solid tumor (Sarcoma 180). The curve of labelled mitosis after pulse labelling with 3H thymidine and the 3H and 14C thymidine double labelling technic on tumor samples incubated in vitro with the labelled precursors were used. A method of digestion of the tissue with hyaluronidase to obtain a cell suspension is described. This method allows easy identification of cells labelled with 3H or 14C thymidine. The two methods yielded reproducible results, the labelling index being 45%, and the duration of S phase 9.9 hours. The in vitro double labelling method with subsequent hyaluronidase digestion is proposed for studying the proliferation kinetics of solid malignancies.

scholarly journals Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia

2003 ◽  
Vol 98 (7) ◽  
pp. 905-908 ◽  
Author(s):  
Wolfan Araque ◽  
Emilia E Barrios ◽  
Pedro Rodríguez ◽  
Víctor S Delgado ◽  
Héctor J Finol
Keyword(s):  
Schistosoma Mansoni ◽  
Ultrastructural Study ◽  
Biomphalaria Glabrata ◽  
In Vitro Interaction

Cell surface modifications in the epithelium of rat ventral prostate during adaptation to in vitro conditions: An ultrastructural study

In Vitro ◽  
1984 ◽  
Vol 20 (3) ◽  
pp. 216-228 ◽  
Author(s):  
Frederick B. Merk ◽  
Paul W. L. Kwan ◽  
Stanley Spilman ◽  
Louis Terracio ◽  
William H. J. Douglas
Keyword(s):  
Cell Surface ◽  
Ultrastructural Study ◽  
Surface Modifications ◽  
Ventral Prostate ◽  
Rat Ventral Prostate

scholarly journals Ultrastructural study of the pineal germinoma in vivo and in vitro

Cancer ◽  
1976 ◽  
Vol 37 (1) ◽  
pp. 327-337 ◽  
Author(s):  
William R. Markesbery ◽  
William H. Brooks ◽  
Larry Milsow ◽  
Richard H. Mortara
Keyword(s):  
Ultrastructural Study ◽  
Pineal Germinoma
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