scholarly journals Exposure to PCBs and p,p ′-DDE and Human Sperm Chromatin Integrity

2005 ◽  
Vol 113 (2) ◽  
pp. 175-179 ◽  
Author(s):  
Anna Rignell-Hydbom ◽  
Lars Rylander ◽  
Aleksander Giwercman ◽  
B.A.G. Jönsson ◽  
Christian Lindh ◽  
...  
Keyword(s):  
Human Sperm ◽  
Sperm Chromatin ◽  
Chromatin Integrity

scholarly journals Toxicants and human sperm chromatin integrity

2009 ◽  
Vol 16 (1) ◽  
pp. 14-22 ◽  
Author(s):  
G. Delbes ◽  
B. F. Hales ◽  
B. Robaire
Keyword(s):  
Human Sperm ◽  
Sperm Chromatin ◽  
Chromatin Integrity

scholarly journals Exposure to PCB and p, p′-DDE in European and Inuit populations: impact on human sperm chromatin integrity

2005 ◽  
Vol 20 (12) ◽  
pp. 3488-3499 ◽  
Author(s):  
M. Spanò ◽  
G. Toft ◽  
L. Hagmar ◽  
P. Eleuteri ◽  
M. Rescia ◽  
...  
Keyword(s):  
Human Sperm ◽  
Sperm Chromatin ◽  
Chromatin Integrity

Perspectives on the assessment of human sperm chromatin integrity

2014 ◽  
Vol 102 (6) ◽  
pp. 1508-1517 ◽  
Author(s):  
Gianpiero D. Palermo ◽  
Queenie V. Neri ◽  
Tyler Cozzubbo ◽  
Zev Rosenwaks
Keyword(s):  
Human Sperm ◽  
Sperm Chromatin ◽  
Chromatin Integrity

scholarly journals Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Marlea Di Santo ◽  
Nicoletta Tarozzi ◽  
Marco Nadalini ◽  
Andrea Borini
Keyword(s):  
Assisted Reproduction ◽  
Human Sperm ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Cryopreservation ◽  
Male Factor ◽  
Assisted Reproduction Technologies ◽  
Chromatin Integrity ◽  
And Function ◽  
Thawing Procedure

Cryopreservation of human spermatozoa—introduced in the 1960's—has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases, vasectomy or surgical infertility treatments, to store donor and partner spermatozoa before assisted reproduction treatments and to ensure the recovery of a small number of spermatozoa in severe male factor infertility. Despite the usefulness of it, cryopreservation may lead to deleterious changes of sperm structure and function: while the effects of cryopreservation on cells are well documented, to date there is no agreement in the literature on whether or not cryopreservation affects sperm chromatin integrity or on the use of a unique and functional protocol for the freezing-thawing procedure. Therefore, sperm cryopreservation is an important component of fertility management and much of its successful application seems to affect the reproductive outcome of assisted reproduction technologies (ART): appropriate use of cryoprotectants before and sperm selection technologies after cryopreservation seem to have the greatest impact on preventing DNA fragmentation, thus improving sperm cryosurvival rates.

scholarly journals Single-center thorough evaluation and targeted treatment of globozoospermic men

2021 ◽  
Author(s):  
Stephanie Cheung ◽  
Alessandra Parrella ◽  
Danielle Tavares ◽  
Derek Keating ◽  
Philip Xie ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Gene Mutations ◽  
Calcium Ionophore ◽  
Immunofluorescence Assay ◽  
Sperm Chromatin ◽  
Reproductive Outcome ◽  
Saint Louis ◽  
Sperm Aneuploidy ◽  
Zygote Development ◽  
Chromatin Integrity

Abstract Purpose To characterize, by specific biomarkers and nucleic acid sequencing, the structural and genomic sperm characteristics of partial (PG) and complete globozoospermic (CG) men in order to identify the best reproductive treatment. Methods We assessed spermatozoa from 14 consenting men ultrastructurally, as well as for histone content, sperm chromatin integrity, and sperm aneuploidy. Additional genomic, transcriptomic, and proteomic evaluations were carried out to further characterize the CG cohort. The presence of oocyte-activating sperm cytosolic factor (OASCF) was measured by a phospholipase C zeta (PLCζ) immunofluorescence assay. Couples were treated in subsequent cycles either by conventional ICSI or by ICSI with assisted gamete treatment (AGT) using calcium ionophore (Ionomycin, 19657, Sigma-Aldrich, Saint Louis, MO, USA). Results Ultrastructural assessment confirmed complete acrosome deficiency in all spermatozoa from CG men. Histone content, sperm chromatin integrity, and sperm aneuploidy did not differ significantly between the PG (n = 4) and CG (n = 10) cohorts. PLCζ assessment indicated a positive presence of OASCF in 4 PG couples, who underwent subsequent ICSI cycles that yielded a 36.1% (43/119) fertilization with a 50% (2/4) clinical pregnancy and delivery rate. PLCζ assessment failed to detect OASCF for 8 CG patients who underwent 9 subsequent ICSI cycles with AGT, yielding a remarkable improvement of fertilization (39/97; 40.2%) (P = 0.00001). Embryo implantation (6/21; 28.6%) and clinical pregnancies (5/7; 71.4%) were also enhanced, resulting in 4 deliveries. Gene mutations (DPY19L2, SPATA16, PICK1) were identified in spermatozoa from CG patients. Additionally, CG patients unable to sustain a term pregnancy had gene mutations involved in zygote development (NLRP5) and postnatal development (BSX). CG patients who successfully sustained a pregnancy had a mutation (PIWIL1) related to sperm phenotype. PLCZ1 was both mutated and underexpressed in these CG patients, regardless of reproductive outcome. Conclusions Sperm bioassays and genomic studies can be used to characterize this gamete’s capacity to support embryonic development and to tailor treatments maximizing reproductive outcome.

Mitochondrial functionality modifies human sperm acrosin activity, acrosome reaction capability and chromatin integrity

2018 ◽  
Vol 34 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Guowei Zhang ◽  
Wang Yang ◽  
Peng Zou ◽  
Fan Jiang ◽  
Yingfei Zeng ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Reaction Capability ◽  
Chromatin Integrity ◽  
Acrosin Activity

Sperm Chromatin Integrity Tests and Indications

2020 ◽  
pp. 99-121
Author(s):  
Rakesh Sharma ◽  
Marlon P. Martinez ◽  
Ashok Agarwal
Keyword(s):  
Sperm Chromatin ◽  
Chromatin Integrity ◽  
Integrity Tests

scholarly journals Influence of PM2.5 on spermatogenesis dysfunction via the reactive-oxygen-species-mediated Mitogen-activated-protein-kinase signaling pathway

2019 ◽  
Vol 6 (4) ◽  
pp. 77-79
Author(s):  
Ruangrong Cheepsattayakorn
Keyword(s):  
Reactive Oxygen Species ◽  
Signaling Pathway ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Mapk Signaling ◽  
Autoimmune Response ◽  
Sperm Chromatin ◽  
Seminiferous Tubules ◽  
Oxygen Species ◽  
Mitogen Activated Protein

Approximately 15 % of the world‘s couples confront childless, and about 50 % of them are due to male reproductive disorders. Several previous studies demonstrated that PM2.5 particles has been consistently associated with critical human sperm reduction and impairment of human sperm chromatin and DNA from traffic exhaust pollution. Blood-testis barrier (BTB), a critically physical barrier between the seminiferous tubules and the blood vessels prevents sperm antigens from entering the blood circulation and facilitating and initiating an autoimmune response that contributing to spermatogenesis interference. Reactive oxygen species (ROS) are involved in the redox-sensitive signal transduction factors activation, such as Jun NH2-terminal kinase (JNK), p 38, extracellular signal-regulated kinase (ERK), and mitogen-activated protein kinases (MAPK) that critically influence BTB disruption. After PM2.5 exposure, there are decreased superoxide dismutase (SOD) expression, increased malondialdehyde (MDA) expression, increased nuclear factor erythroid 2-related factor 2 (Nrf-2) expression, increased expression of the four junctional proteins (β-catenin, Cx43, occludin, zonula occludens-1 (ZO-1)), thus improve sperm quality and quantity. PM2.5 particles markedly induce increasing phosphorylation of MAPKs via the ROS-mediated MAPK signaling pathway that causes BTB disruption, but this effect is lesser in the vitamins C and E intervention as well as increasing cleaved caspase-3 expression and the Bcl-2/Bax ratio. In conclusion, combined therapeutic administration of vitamins C and E can maintain the BTB integrity, reduce oxidative stress and cell apoptosis, and prevent toxic effects.

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