Mitochondrial functionality modifies human sperm acrosin activity, acrosome reaction capability and chromatin integrity

2018 ◽  
Vol 34 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Guowei Zhang ◽  
Wang Yang ◽  
Peng Zou ◽  
Fan Jiang ◽  
Yingfei Zeng ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Reaction Capability ◽  
Chromatin Integrity ◽  
Acrosin Activity

The effect of smoking and varicocele on human sperm acrosin activity and acrosome reaction

1995 ◽  
Vol 10 (12) ◽  
pp. 3190-3194 ◽  
Author(s):  
K.F.El Mulla ◽  
F.-M. K¨ohn ◽  
A.H.El Beheiry ◽  
W.-B. Schill
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Acrosin Activity

scholarly journals The Laminin-Induced Acrosome Reaction in Human Sperm Is Mediated by Src Kinases and the Proteasome1

2011 ◽  
Vol 85 (2) ◽  
pp. 357-366 ◽  
Author(s):  
Silvia Tapia ◽  
Marcelo Rojas ◽  
Patricio Morales ◽  
Marco A. Ramirez ◽  
Emilce S. Diaz
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Src Kinases

Mechanism of human sperm activation by extracellular ATP

1996 ◽  
Vol 270 (6) ◽  
pp. C1709-C1714 ◽  
Author(s):  
C. Foresta ◽  
M. Rossato ◽  
P. Chiozzi ◽  
F. Di Virgilio
Keyword(s):  
Plasma Membrane ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Extracellular Atp ◽  
Effective Concentration ◽  
Na Channel ◽  
Monovalent Cations ◽  
Sperm Activation ◽  
Gated Channel ◽  
Sperm Plasma Membrane

We have identified the mechanism whereby extracellular ATP (ATPe) triggers the acrosome reaction in human spermatozoa. This nucleotide opens a ligand-gated ion channel expressed on the sperm plasma membrane. ATPe threshold and 50% effective concentration calculated on the total added ATPe are 0.1 and 2 mM, respectively, corresponding to a free ATP concentration (ATP4-) of 3 and 200 microM, respectively. The ATPe-gated channel is selective for monovalent cations (Na+, choline, and methylglucamine), whereas on the contrary, permeability to Ca2+ is negligible. Isosmolar replacement of extracellular Na+ with sucrose fully blocked ATPe-dependent sperm activation, thus suggesting a mandatory role for Na+ influx. These results show that human sperm express an ATPe-gated Na+ channel that might have an important role in sperm activation before egg fertilization.

scholarly journals Soluble adenylyl cyclase inhibition prevents human sperm functions essential for fertilization

2021 ◽  
Author(s):  
Melanie Balbach ◽  
Lubna Ghanem ◽  
Thomas Rossetti ◽  
Navpreet Kaur ◽  
Carla Ritagliati ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Comprehensive Analysis ◽  
Multiple Points ◽  
Soluble Adenylyl Cyclase ◽  
Mouse Sperm ◽  
On Demand ◽  
Sperm Functions

AbstractSoluble adenylyl cyclase (sAC: ADCY10) is essential for activating dormant sperm. Studies of freshly dissected mouse sperm identified sAC as needed for initiating capacitation and activating motility. We now use an improved sAC inhibitor, TDI-10229, for a comprehensive analysis of sAC function in human sperm. Unlike dissected mouse sperm, human sperm are collected post-ejaculation, after sAC activity has already been stimulated. Even in ejaculated human sperm, TDI-10229 interrupts stimulated motility and capacitation, and it prevents acrosome reaction in capacitated sperm. At present, there are no non-hormonal, pharmacological methods for contraception. Because sAC activity is required post-ejaculation at multiple points during the sperm’s journey to fertilize the oocyte, sAC inhibitors define candidates for non-hormonal, on-demand contraceptives suitable for delivery via intravaginal devices in females.

scholarly journals Discrete Dynamic Model of the Mammalian Sperm Acrosome Reaction: The Influence of Acrosomal pH and Physiological Heterogeneity

2021 ◽  
Vol 12 ◽  
Author(s):  
Andrés Aldana ◽  
Jorge Carneiro ◽  
Gustavo Martínez-Mekler ◽  
Alberto Darszon
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Extracellular Medium ◽  
Mammalian Sperm ◽  
Discrete Dynamic Model ◽  
Acrosomal Exocytosis ◽  
Mammalian Fertilization ◽  
Main Components ◽  
Discrete Mathematical Model ◽  
Physiological Heterogeneity

The acrosome reaction (AR) is an exocytotic process essential for mammalian fertilization. It involves diverse physiological changes (biochemical, biophysical, and morphological) that culminate in the release of the acrosomal content to the extracellular medium as well as a reorganization of the plasma membrane (PM) that allows sperm to interact and fuse with the egg. In spite of many efforts, there are still important pending questions regarding the molecular mechanism regulating the AR. Particularly, the contribution of acrosomal alkalinization to AR triggering physiological conditions is not well understood. Also, the dependence of the proportion of sperm capable of undergoing AR on the physiological heterogeneity within a sperm population has not been studied. Here, we present a discrete mathematical model for the human sperm AR based on the physiological interactions among some of the main components of this complex exocytotic process. We show that this model can qualitatively reproduce diverse experimental results, and that it can be used to analyze how acrosomal pH (pHa) and cell heterogeneity regulate AR. Our results confirm that a pHa increase can on its own trigger AR in a subpopulation of sperm, and furthermore, it indicates that this is a necessary step to trigger acrosomal exocytosis through progesterone, a known natural inducer of AR. Most importantly, we show that the proportion of sperm undergoing AR is directly related to the detailed structure of the population physiological heterogeneity.

scholarly journals Expression and function of ras proto-oncogene proteins in human sperm cells

1992 ◽  
Vol 102 (3) ◽  
pp. 487-494 ◽  
Author(s):  
R.K. Naz ◽  
K. Ahmad ◽  
P. Kaplan
Keyword(s):  
Sperm Cell ◽  
Acrosome Reaction ◽  
Cell Function ◽  
Beat Frequency ◽  
Human Sperm ◽  
Sperm Cells ◽  
Ras Protein ◽  
Head Displacement ◽  
Sperm Penetration ◽  
And Function

The presence and role of c-ras proteins were investigated in mature human sperm cells. The v-H-ras monoclonal antibody (mAb) against the c-ras protein, p21, reacted specifically with the acrosomal region of methanol-fixed as well as unfixed-live capacitated and non-capacitated human sperm cell in the indirect immunofluorescence technique. The v-H-ras mAb predominantly recognized c-ras protein of 21 kDa on the Western blot of lithium diiodosalicylate (LIS)-solubilized human sperm preparation. The incubation of sperm cells with v-H-ras mAb affected the sperm cell function in the human sperm penetration assay. The antibody significantly reduced the acrosome reaction and release of acrosin activity from the sperm cells. There was no effect of the mAb on percentage motility, although the mAb significantly affected various motility characteristics such as linearity, amplitude of lateral head displacement (ALH) and beat frequency, the motility parameters involved in the hyperactivation phenomenon of sperm cells leading to capacitation and acrosome reaction. These results suggest that the c-ras or c-ras-like proteins are present in mature sperm cell and may have a role in capacitation and/or acrosome reaction of human sperm cell.

scholarly journals Human Sperm Chromatin Undergoes Physiological Remodeling During In Vitro Capacitation and Acrosome Reaction

2012 ◽  
Vol 33 (5) ◽  
pp. 1025-1035 ◽  
Author(s):  
E. de Lamirande ◽  
M. C. San Gabriel ◽  
A. Zini
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Sperm Chromatin

Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm

1994 ◽  
Vol 39 (3) ◽  
pp. 280-288 ◽  
Author(s):  
Patricia Saragüeta ◽  
Guillermo Lanuza ◽  
Patricia V. Miranda ◽  
Jorge G. Tezón ◽  
J. Lino Baraño
Keyword(s):  
Follicular Fluid ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Human Follicular Fluid
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