scholarly journals The clinically relevant diabetic foot syndrome morphology

2021 ◽  
Vol 11 (5) ◽  
pp. 262-274
Author(s):  
V. Polyovyy ◽  
Bilel Khorshani ◽  
R. Sydorchuk ◽  
P. Kyfiak ◽  
O. Khomko ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Morphological Changes ◽  
Cellular Level ◽  
Molecular Structures ◽  
Diabetic Foot Syndrome ◽  
Inflammatory Infiltration ◽  
Diffuse Infiltration ◽  
Tissue Ischemia ◽  
Tissue Edema ◽  
The Status

Objective: to evaluate the morphological changes in diabetic foot syndrome emphasizing the ultrastructural cellular changes.Material and methods. Biopsies of the chronic lesions in 120 diabetic foot patients following necessary preparatory stages were studied by histological, histochemical and electron microscopy methods.Results. The inflammatory infiltration and destruction and involving all layers of skin and soft tissue, the phenomena of vacuolation, apoptosis or karyolysis; tissue edema, diffuse infiltration by neutrophils, vascular plethora with stasis, thrombosis, and necrosis were observed. Microcirculatory tract thrombosis exacerbated tissue ischemia, contributing to the progression of pathology. The macrophages' cytoplasm characterized by the presence of phagocytosed and digested microorganisms often intact inside them. The changes in the status of nuclear and perinuclear material were accompanied by compression and dehydration of the cell, which ended in its fragmentation and the formation of tightly contacting bodies of various shapesConclusions. Morphological changes in diabetic foot syndrome are diverse and uneven, occurring not only on tissue and cellular level but involves subcellular and molecular structures, encouraging further research in this field. Foci of destruction and lysis of the newly formed epithelium are characteristic for diabetic foot, which is not observed in the normal wound process.

scholarly journals The significance of electrothermometry indices for organization of adequate infusion therapy in diabetic foot syndrome

2012 ◽  
Vol 93 (2) ◽  
pp. 389-390
Author(s):  
R I Fatykhov ◽  
I V Klyushkin ◽  
L A Akhmetyanov
Keyword(s):  
Clinical Data ◽  
Diabetic Foot ◽  
Conservative Therapy ◽  
Dynamic Control ◽  
Diagnostic Procedures ◽  
Lower Limbs ◽  
Diabetic Foot Syndrome ◽  
Infusion Therapy ◽  
Subsequent Formation ◽  
Tissue Ischemia

Aim. To determine the criteria for conservative infusion therapy with the assessment of its effectiveness using the electrothermometry method. Methods. One of the major complications of diabetes is the diabetic foot syndrome. This diagnosis is established on the basis of clinical data and imaging studies. The resulting parameters are objective, but the specificity, selectivity, and the multiplicity of diagnostic procedures negate the effectiveness of these methods, making it difficult to consequently form treatment strategy. Registration of temperature parameters during different degrees of ischemic disturbances of the lower limbs by electrothermometry was performed with the subsequent formation of the algorithm of conservative therapy. Results. In patients with diabetic foot syndrome determined was the direct correlation between the degree of tissue ischemia of the lower extremities and the variation of temperature parameters. The formation of the course of conservative therapy was performed after evaluating the obtained parameters; its formation was based on the main pathogenetic links. Clinical data and the parameters of electrothermometry served as the method of dynamic control. Conclusion. Despite the achieved successes in the modern diagnosis of the diabetic foot syndrome, there still remains a number of inaccuracies, the key ones among then are the difficulties associated with determining the level of trophic disorders; electrothermometry makes it possible to expand the diagnostic horizons, and the obtained result can serve as a prognostic guide for the formation of the tactics of therapy and as a method of dynamic control.

Patho-morphological changes of tissue of hypervascular formation at the endovascular treatment

2015 ◽  
Vol 9 (1) ◽  
pp. 0-0
Author(s):  
Ольшанский ◽  
M. Olshanskiy ◽  
Харитонов ◽  
D. Kharitonov ◽  
Степанов ◽  
...  
Keyword(s):  
Blood Flow ◽  
Multidisciplinary Approach ◽  
Morphological Changes ◽  
Endovascular Embolization ◽  
Ischemic Necrosis ◽  
Inflammatory Infiltration ◽  
Cavernous Malformations ◽  
Tissue Ischemia ◽  
Surgical Treatments ◽  
Further Development

Treatment of hypervascular formations is challenging surgery. The solution of this problem is due to use a multidisciplinary approach, involving complex endovascular and surgical treatments. Patho-morphological study of remote hypervascular formations in treatment was carried out. That treatment consisted of an endovascular embolotherapy and an endovasal sclerotherapy in various combinations. The embolization of blood vessels leads to a complete reduction of blood flow in the formation area, the development of diffuse inflammatory infiltration in the background on tissue ischemia. In the future, developing ischemic necrosis fol-lowed by scarring of tissue surrounding the vascular formation. Sclerotherapy causes damage to the vascular endothelium with fibrin formation and hemolysis of red blood cells in the lumen of the veins. Moreover, there are a significant fibrosis, hyalinosis and inflammatory reaction of the tissues surrounding the vessels. Further development of this process leads to a final sclerosis and fibrotic scarring of the surrounding tissues. The combi-nation of endovascular embolization and sclerotherapy allows to achieve a reduction of blood flow in the arterial vascular malformation department and to cause a tissue fibrosis in the cavernous malformations. Endovascular therapy reduces the risk of bleeding in surgery and develops the capacity of radical treatment.

scholarly journals The morphological characteristic of tissue repair in patients with neuropathic and neuroischemic forms of diabetic foot syndrome

2015 ◽  
Vol 18 (4) ◽  
pp. 72-78 ◽  
Author(s):  
Ekaterina L. Zaitseva ◽  
Ludmila P. Doronina ◽  
Roman V. Molchkov ◽  
Iya A. Voronkova ◽  
Alla Y. Tokmakova
Keyword(s):  
Diabetes Mellitus ◽  
Granulation Tissue ◽  
Diabetic Foot ◽  
Tissue Repair ◽  
Diabetic Foot Syndrome ◽  
Inflammatory Infiltration ◽  
Intense Staining ◽  
Repair Processes ◽  
Patients With Diabetes ◽  
Immunohistochemical Studies

Aim. To evaluate the morphological and immunohistochemical features of granulation tissue formation in patients with diabetic foot syndrome.Materials and methods. We analysed the histological (light microscopy) and immunohistochemical (CD31, CD68, osteopontin, MMP-9 and TIMP-1) features of tissue repair processes in patients with diabetes mellitus. The study involved 63 patients with diabetic foot syndrome after surgical debridement.Results. We found severe intercellular oedema, poorly organised extracellular matrix, small amounts of fibroblast-like cells and expressed inflammatory infiltration, along with the presence of young granulation tissue. According to the results of the immunohistochemical studies, there were a moderate number of macrophages (immunopositive with antibodies to CD68), intense staining of MMP-9 and weak staining of TIMP-1 and osteopontin.Conclusion. According to the findings of the histological and immunohistochemical studies, tissue repair processes in patients with diabetes mellitus are decelerated.

Fate of sperm membrane in fertilized ova

1993 ◽  
Vol 51 ◽  
pp. 40-41
Author(s):  
Frank J. Longo
Keyword(s):  
Electron Microscopy ◽  
Electrical Activity ◽  
Sea Urchins ◽  
Morphological Changes ◽  
Integrated System ◽  
Electrophysiological Recording ◽  
Experimental Conditions ◽  
The Status ◽  
Sperm Membrane ◽  
Temporal And Spatial

Measurement of the egg's electrical activity, the fertilization potential or the activation current (in voltage clamped eggs), provides a means of detecting the earliest perceivable response of the egg to the fertilizing sperm. By using the electrical physiological record as a “real time” indicator of the instant of electrical continuity between the gametes, eggs can be inseminated with sperm at lower, more physiological densities, thereby assuring that only one sperm interacts with the egg. Integrating techniques of intracellular electrophysiological recording, video-imaging, and electron microscopy, we are able to identify the fertilizing sperm precisely and correlate the status of gamete organelles with the first indication (fertilization potential/activation current) of the egg's response to the attached sperm. Hence, this integrated system provides improved temporal and spatial resolution of morphological changes at the site of gamete interaction, under a variety of experimental conditions. Using these integrated techniques, we have investigated when sperm-egg plasma membrane fusion occurs in sea urchins with respect to the onset of the egg's change in electrical activity.

Screening of Kazakhstan plants useful in the treatment of diabetic foot syndrome

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
TS Kustova ◽  
LK Mamonov ◽  
CL Cantrell ◽  
SA Ross
Keyword(s):  
Diabetic Foot ◽  
Diabetic Foot Syndrome

Improvement of specialised help to patient with diabetic foot syndrome in Uzbekistan

2017 ◽  
Author(s):  
Nilufar Ibragimova ◽  
Telman Kamalov ◽  
Hamidulla Shakirov ◽  
Oxana Platonova ◽  
Lyudmila Kokareva
Keyword(s):  
Diabetic Foot ◽  
Diabetic Foot Syndrome

scholarly journals The diabetic foot syndrome - therapy

2004 ◽  
Vol 129 (14) ◽  
pp. 751-754 ◽  
Author(s):  
H P Lorenzen ◽  
H Schunkert
Keyword(s):  
Diabetic Foot ◽  
Diabetic Foot Syndrome
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