scholarly journals Effect of Cryoprotectant Gradient Equilibration Method on In Vitro Survival Rate and Pregnancy Rate of Bovine IVF Blastocysts.

2001 ◽  
Vol 18 (2) ◽  
pp. 67-71
Author(s):  
Yutaka Kajihara ◽  
Atsurou Hira ◽  
Ryouhei Nitta ◽  
Yoshihiko Nakanishi
Keyword(s):  
Survival Rate ◽  
Pregnancy Rate ◽  
In Vitro Survival

scholarly journals Correction to: High in vitro survival rate of sheep in vitro produced blastocysts vitrified with a new method and device

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Sergio Ledda ◽  
Jen M. Kelly ◽  
Stefano Nieddu ◽  
Daniela Bebbere ◽  
Federica Ariu ◽  
...  
Keyword(s):  
Survival Rate ◽  
New Method ◽  
Original Publication ◽  
In Vitro Survival

In the original publication of this article [1], the author point out an error in Fig. 3. The correct Fig. 3 is below.

scholarly journals High in vitro survival rate of sheep in vitro produced blastocysts vitrified with a new method and device

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Sergio Ledda ◽  
Jen M. Kelly ◽  
Stefano Nieddu ◽  
Daniela Bebbere ◽  
Federica Ariu ◽  
...  
Keyword(s):  
Survival Rate ◽  
Survival Rates ◽  
Blastocyst Stage ◽  
Hatching Rate ◽  
High Survival ◽  
Apoptotic Cells ◽  
Embryo Survival ◽  
New Device ◽  
In Vitro Survival

Abstract Background To advance the use of embryo vitrification in veterinary practice, we developed a system in which embryo vitrification, warming and dilution can be performed within a straw. Ovine in vitro produced embryos (IVEP) were vitrified at either early (EBs: n = 74) or fully expanded blastocyst stage (FEBs: n = 195), using a new device named “E.Vit”, composed by a 0.25-mL straw with a 50-μm pore polycarbonate grid at one end. Embryos at each stage (EBs and FEBs) were vitrified by either Two-step (TS) or Multi-step (MS; 6 different concentrations of vitrification solutions) protocol. Non-vitrified embryos (n = 102) were maintained in in vitro culture as a control. Warming consisted of placing the straws directly into 1.5 mL tubes containing a TCM-199 solution with three decreasing concentrations of sucrose. Blastocyst re-expansion, embryo survival and hatching rate were evaluated at 2, 24 and 48 h post warming. The number of apoptotic cells was determined by TUNEL assay. Results Blastocyst re-expansion (2 h) after warming was higher (P < 0.05) in FEBs group, vitrified with the MS and TS methods (77.90% and 71.25%, respectively) compared with the EBs group (MS: 59.38% and TS: 48.50%, respectively). Survival rates of vitrified FEBs after 24 h IVC were higher (P < 0.001) in both methods (MS and TS) than vitrified EBs (MS: 56.25%; TS: 42.42%) and was higher (P < 0.05) in the MS method (94.19%) compared with those in TS (83.75%). After 48 h of culture the hatching rate for FEBs vitrified in MS system (91.86%) was similar to control (91.89%), but higher than FEB TS (77.5%) and EBs vitrified in MS (37.5%) and TS (33.33%). Number of apoptotic cells were higher in EBs, irrespective of the system used, compared to FEBs. The number of apoptotic cells in FEBs vitrified with MS was comparable to the control. Conclusions A high survival rate of IVP embryos can be achieved by the new “E.Vit” device with hatching rates in vitro comparable with control fresh embryos. This method has the potential for use in direct embryo transfer in field conditions.

Effect of the pH of freezing medium on in vitro survival rate of bovine blastocysts after freezing and thawing

1993 ◽  
Vol 39 (1) ◽  
pp. 240 ◽  
Author(s):  
Y. Kajihara ◽  
A. Hira ◽  
H. Fukudome ◽  
K. Hishiyama ◽  
M. Endo ◽  
...  
Keyword(s):  
Survival Rate ◽  
Freezing And Thawing ◽  
Freezing Medium ◽  
In Vitro Survival

Rapid cryopreservation of sheep embryos by direct transfer into liquid nitrogen vapour at -180 degrees C

1990 ◽  
Vol 2 (6) ◽  
pp. 613 ◽  
Author(s):  
A Szell ◽  
J Zhang ◽  
R Hudson
Keyword(s):  
Survival Rate ◽  
Propylene Glycol ◽  
Liquid Nitrogen ◽  
Direct Transfer ◽  
Rate Of Development ◽  
Rapid Procedure ◽  
In Vitro Survival ◽  
Cryopreserved Embryos

The in vitro survival of control and rapidly cryopreserved sheep embryos was examined as a function of the duration of exposure to a vitrification medium (25% glycerol + 25% propylene glycol). Embryos in late morula to late blastocyst stages were permeated by a mixture of 10% glycerol + 20% propylene glycol for 10 min at 18-23 degrees C and then exposed to the vitrification medium for 0.5, 1, 2 or 4 min at 18-23 or 4-12 degrees C. The cryoprotectants were removed without cryopreservation (control embryos) or after rapid cryopreservation by direct transfer into liquid nitrogen vapour at -180 degrees C. The duration of exposure to the vitrification medium at 18-23 degrees C affected the in vitro survival rate of control embryos (P = 0.06) but had no effect on the survival of rapidly cryopreserved embryos. However, at 4-12 degrees C the duration of exposure affected the survival of cryopreserved embryos (0.5 min: 64%, 18/28; 1-4 min: 43%, 34/80; P = 0.074). Overall, the in vitro survival rate of control and cryopreserved embryos increased with advancing development from late morulae (36%) to late blastocysts (70%). The in vivo survival of embryos that had been exposed to the vitrification medium for 0.5 min at 4-12 degrees C and then vitrified was tested. The rate of development to term was 11% (4/35) for late morulae or early blastocysts and 32% (6/19; P greater than 0.1) for blastocysts to hatching blastocysts. These results showed that sheep embryos can be successfully cryopreserved by a simple, rapid procedure.

70 EFFECT OF CRYOPROTECTANT CONCENTRATION ON THE IN VITRO SURVIVAL AND CELL PROLIFERATION OF PORCINE BLASTOCYSTS VITRIFIED USING THE OPEN PULLED STRAW SYSTEM

2008 ◽  
Vol 20 (1) ◽  
pp. 116
Author(s):  
C. Cuello ◽  
J. Sanchez-Osorio ◽  
C. Almiñana ◽  
M. A. Gil ◽  
I. Caballero ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Survival Rate ◽  
Nuclear Antigen ◽  
Proliferation Index ◽  
Hatching Rate ◽  
Hoechst 33342 ◽  
Number Range ◽  
Significant Difference ◽  
In Vitro Survival

The objective of the present project was to study the effect of the concentrations of ethylene glycol (EG) and dimethyl sulfoxide (DMSO) during vitrification on the survival and hatching rates of porcine blastocysts. Embryos were collected by laparotomy from weaned crossbred sows (n = 18), vitrified, and warmed (one-step dilution) as described by Cuello et al. (2004 Theriogenology 62, 1144–1152). Vitrification was performed in different concentrations of EG and DMSO (15%, 16%, and 17% v/v for each cryoprotectant) or in an EG-based medium (40% v/v) using superfine open pulled straws. Fresh and vitrified blastocysts were cultured for 24 h in TCM199 and assessed by stereomicroscopy during the culture. Blastocysts that reformed their blastocoelic cavities after warming, displaying a normal zona pellucida and excellent appearance, were considered viable. The in vitro survival rate was defined as the ratio of viable embryos divided by the total number of embryos cultured. The hatching rate is determined as the ratio of the number of embryos hatched in vitro to the total number embryos cultured. Some vitrified and fresh embryos classified as viable were processed for Hoechst 33342 staining and proliferation cell nuclear antigen (PCNA) inmunolocalization. The proliferation index was defined as the number of PCNA-positive nuclei divided by the total number of nuclei stained with Hoechst 33342. Data were analyzed by ANOVA using the MIXED procedure (SPSS version 11.5; SPSS, Inc., Chicago, IL, USA). Data were expressed as mean values � SEM. The survival rate was similar for fresh and vitrified blastocysts, except for blastocysts vitrified using 15% cryoprotectants, which displayed a lower (P < 0.05) survival rate (84.2 � 4.8%) than fresh blastocysts (94.6 � 5%) and blastocysts vitrified using 40% EG, 16%, or 17% EG-DMSO (88.8 � 4.9, 96.8 � 4.9, and 96.4 � 5%, respectively). Fresh blastocysts showed a higher (P < 0.05) hatching rate (80.7 � 4.5%) than their vitrified counterparts (range: 48.4 � 7.7–55.3 � 7.8%). Vitrified and fresh blastocysts showed similar cell proliferation indexes (range: 75.8 � 3.2–83.7 � 3). When only hatched blastocysts among groups were compared, the proliferation rate decreased (P < 0.05) after vitrification with 17% EG-DMSO. Among vitrification groups, there was no significant difference in the number of total cells. However, vitrified blastocysts had a lower (P < 0.05) total cell number (range: 116.6 � 6.7–124.8 � 6.6) than fresh blastocysts (195.5 � 11.4). In conclusion, under our experimental conditions, the concentration of EG-DMSO can be decreased until 16% in the vitrification medium with no reduction of the in vitro developmental ability of the blastocysts. In addition, a 40% EG-based medium can be used for vitrification with results similar to those achieved using a medium containing 16% EG-DMSO.

scholarly journals In vitro mycorrhization of micropropagated Helianthemum almeriense plantlets with Terfezia claveryi (desert truffle)

1994 ◽  
Vol 3 (3) ◽  
pp. 309-314 ◽  
Author(s):  
Maria Asunción Morte ◽  
Antonio Cano ◽  
Mario Honrubia ◽  
Pilar Torres
Keyword(s):  
Survival Rate ◽  
Mineral Composition ◽  
Agar Medium ◽  
In Vitro Rooting ◽  
Desert Truffle ◽  
Limiting Step ◽  
Mycorrhizal Inoculation ◽  
In Vitro Survival ◽  
Terfezia Claveryi

The mycorrhizal synthesis of Terfezia claveryi with micropropagated plantlets of Helianthemum almeriense was carried out in vitro on modified Modified Melin- Norkrans (MMN) agar medium with pH 8.0. The mycorrhization rate was about 80% after 12 weeks. T. claveryi formed ectendomycorrhizas without hyphal mantle. The effect of the fungus on in vitro rooting was also studied. T. claveryi did not enhance in vitro rooting of microcuttings of H. almeriense. In vitro survival of the plantlets was the limiting step and mycorrhizal inoculation appeared to improve the survival rate of rooted plantlets. The effect of the mineral composition and pH of the medium on survival are discussed.

Combination Therapy of Cisplatin and Other Agents for Osteosarcoma: A Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Mohamad Zahid Kasiram ◽  
Hermizi Hapidin ◽  
Hasmah Abdullah ◽  
Azlina Ahmad ◽  
Sarina Sulong
Keyword(s):  
Radiation Therapy ◽  
Survival Rate ◽  
Chemotherapeutic Agents ◽  
New Combination ◽  
Rapid Progression ◽  
Combination Regimen ◽  
Chemotherapeutic Drugs ◽  
Primary Bone Tumor ◽  
Phytochemical Compounds

Background: Osteosarcoma is the most common type of primary bone tumor in children and adolescents, which is associated with rapid progression and poor prognosis. Multimodal therapy is the most common approach utilized for osteosarcoma management, such as the application of chemotherapy in combination with surgery or radiation therapy. Cisplatin is one of the predominantly used chemotherapeutic agents for osteosarcoma. Optimally, it is employed in combination with other chemotherapeutic drugs along with surgery or radiation therapy. Despite the availability of numerous treatment approaches, patient survival rate has not definitively improved over the past three decades. Methods: We summarized all findings regarding the combination of cisplatin with other chemotherapeutic agents as well as with phytochemical compounds. Results: A combination of cisplatin with phytochemical compound synergistically enhances the killing effect of cisplatin on osteosarcoma cells with fewer side effects compared to combination with other chemotherapeutic agents. Conclusion: Conclusively, a combination of cisplatin with selected chemotherapeutic drugs, has been shown to be effective. However, the unchanged survival rate urges for the search of a new combination regimen. As a collaborative effort to substantiate the therapeutic efficacy, the combination with phytochemical compounds shows a promising response both in vitro as well as in the preclinical study.

scholarly journals The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease

2017 ◽  
Vol 5 (2) ◽  
pp. 1
Author(s):  
Mulyati Mulyati ◽  
Suryati Suryati ◽  
Irfani Baga
Keyword(s):  
Survival Rate ◽  
Sea Water ◽  
Challenge Test ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Inhibitory Ability ◽  
Significant Difference ◽  
Portunid Crab

The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.

Sign in / Sign up

Export Citation Format

Share Document