scholarly journals In Vitro Culture of Pig Oocytes Collected from Early Antral Follicles.

1998 ◽  
Vol 15 (3) ◽  
pp. 161-166 ◽  
Author(s):  
Takashi Miyano ◽  
Sadanobu Moritake ◽  
Kazumasa Hirata ◽  
Masashi Miyake ◽  
Seishiro Kato
Keyword(s):  
In Vitro Culture ◽  
Antral Follicles

In vitro culture of secondary follicles and prematuration of cumulus–oocyte complexes from antral follicles increase the levels of maturation‐related transcripts in bovine oocytes

2019 ◽  
Vol 86 (12) ◽  
pp. 1874-1886
Author(s):  
Francisco Taiã G. Bezerra ◽  
Francisco Edilcarlos O. Lima ◽  
Laís Rayani F. M. Paulino ◽  
Bianca R. Silva ◽  
Anderson W. B. Silva ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Antral Follicles ◽  
Bovine Oocytes

In vitro culture of isolated preantral and antral follicles of goats using human recombinant FSH: Concentration-dependent and stage-specific effect

2018 ◽  
Vol 196 ◽  
pp. 120-129 ◽  
Author(s):  
Anna Clara A. Ferreira ◽  
Jesús Cadenas ◽  
Naiza A.R. Sá ◽  
Hudson H.V. Correia ◽  
Denise D. Guerreiro ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Specific Effect ◽  
Recombinant Fsh ◽  
Antral Follicles

Effect of medium composition on the in vitro culture of bovine pre-antral follicles: morphology and viability do not guarantee functionality

Zygote ◽  
2012 ◽  
Vol 21 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Rafael Rossetto ◽  
Márcia Viviane Alves Saraiva ◽  
Regiane Rodrigues dos Santos ◽  
Cleidson Manoel Gomes da Silva ◽  
Luciana Rocha Faustino ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Media ◽  
Medium Composition ◽  
Minimum Essential Medium ◽  
Antral Follicles ◽  
Suitable Medium

SummaryThis study investigated the effect of three different culture media (α minimum essential medium (α-MEM), McCoy or TCM199 during the in vitro culture (IVC) of bovine isolated pre-antral follicles. Pre-antral follicles greater than 150 μm in size were isolated and cultured for 0 (control), 8 or 16 days in one of the abovementioned culture media. Follicles were evaluated for survival, growth and antrum formation at days 8 and 16. The results showed that TCM199 was the most suitable medium to preserve follicular viability and ultrastructure, resulting in the highest rates of antrum formation. In conclusion, TCM199 promotes the in vitro development of isolated pre-antral follicles without hampering follicular functionality by sustaining in vitro growth and antrum formation.

Oocyte maturation and expression pattern of follicular genes during in-vitro culture of vitrified mouse pre-antral follicles

2016 ◽  
Vol 20 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Parisa Jamalzaei ◽  
Mojtaba Rezazadeh Valojerdi ◽  
Bita Ebrahimi ◽  
Ali Farrokhi
Keyword(s):  
In Vitro Culture ◽  
Expression Pattern ◽  
Oocyte Maturation ◽  
Antral Follicles

Cryopreservation and in vitro culture of caprine preantral follicles

2011 ◽  
Vol 23 (1) ◽  
pp. 40 ◽  
Author(s):  
J. R. Figueiredo ◽  
A. P. R. Rodrigues ◽  
J. R. V. Silva ◽  
R. R. Santos
Keyword(s):  
Endangered Species ◽  
In Vitro Culture ◽  
Young Women ◽  
Reproductive Potential ◽  
Slow Freezing ◽  
Preantral Follicles ◽  
Antral Follicles ◽  
Large Numbers ◽  
In Vitro Embryo Production

Preantral follicles (PFs) form a far larger oocyte reservoir (∼90% of the follicular population) than antral follicles. Several laboratories have focussed efforts on cryopreservation and in vitro culture (IVC) of PFs to obtain large numbers of fertilisable oocytes. This technology could be used to improve the reproductive potential of economically important animals, including goats, to preserve endangered species and breeds and improve fertility after chemotherapy in young women. Caprine PFs have been successfully cryopreserved using either vitrification or slow freezing. In addition, in vitro embryo production from oocytes enclosed in caprine PFs grown and matured in vitro was also achieved. The present paper selectively reviews the published studies on cryopreservation and IVC of caprine PFs to highlight advances, limitations and prospects.

First pregnancy after in vitro culture of early antral follicles in goats: Positive effects of anethole on follicle development and steroidogenesis

2020 ◽  
Vol 87 (9) ◽  
pp. 966-977
Author(s):  
Naiza A. R. Sá ◽  
Anna C. A. Ferreira ◽  
Francisca G. C. Sousa ◽  
Ana B. G. Duarte ◽  
Victor. M. Paes ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Follicle Development ◽  
Antral Follicles ◽  
Positive Effects

In vitro culture of rat pre-antral follicles with emphasis on follicular interactions

2000 ◽  
Vol 55 (1) ◽  
pp. 65-74 ◽  
Author(s):  
J. Zhao ◽  
M. Dorland ◽  
M.A.M. Taverne ◽  
G.C. Van Der Weijden ◽  
M.M. Bevers ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Antral Follicles

scholarly journals Toxicity effect of Auxemma oncocalyx fraction and its active principle oncocalyxone A on in vitro culture of caprine secondary follicles and in vitro oocyte maturation

2017 ◽  
Vol 38 (3) ◽  
pp. 1361 ◽  
Author(s):  
Johanna Leiva-Revilla ◽  
Jesús Cadenas ◽  
Luis Alberto Vieira ◽  
Claudio Cabral Campello ◽  
Juliana Jales de Hollanda Celestino ◽  
...  
Keyword(s):  
Growth Rate ◽  
In Vitro Culture ◽  
Oocyte Maturation ◽  
In Vitro Maturation ◽  
Cellular Proliferation ◽  
Control Group ◽  
Antral Follicles ◽  
Chromatin Configuration ◽  
In Vitro Oocyte Maturation

Crude extract of the heartwood of Auxemma oncocalyx (A. oncocalyx) and its main component i.e., Oncocalyxone A (onco A), have elevated antioxidant and anti-tumoral activity, but studies on the action of these drugs regarding folliculogenesis are lacking. The aim of this study was to evaluate the effect of A. oncocalyx and onco A on the in vitro culture of isolated secondary follicles and on the in vitro maturation of oocytes from caprine antral follicles grown in vivo. Isolated secondary follicles were randomly distributed in six groups; the non-cultured control was immediately fixed upon isolation. The remaining follicles were cultured for 7 days in ?-MEM+ alone (control) or supplemented with DMSO, doxorrubicin, A. oncocalyx or onco A. After culture, follicles were evaluated for antrum formation, growth rate, apoptosis (TUNEL) and cellular proliferation (PCNA), as well as gene expression of Bcl2 and Bax. Additionally, cumulus oocyte complexes (COCs) were aspirated and allocated into five treatments for in vitro maturation: control, cultured only in maturation base medium (TCM 199+); or supplemented with DMSO; DXR; A. oncocalyx or onco A. After in vitro maturation, oocyte chromatin configuration and viability were assessed. After 7 days of culture, there was a reduction (P < 0.05) in the percentage of morphologically intact follicles, antrum formation, growth rate and number of PCNA positive granulosa cells in DXR treatment compared to the other treatments. In the DXR treatment a higher percentage (P < 0.05) of TUNEL positive follicles and higher (P < 0.05) relative BAX:BCL2 mRNA ratio’s were observed. After in vitro maturation of the COCs DXR, A. oncocalyx and onco A treatments had a greater (P < 0.05) percentage of abnormal oocytes and a lower (P < 0.05) percentage of viable oocytes as compared with the control group. However, only DXR and onco A treatments increased (P < 0.05) the percentage of alive oocytes with abnormal chromatin configuration. There were no differences in maturation rates between the control group and DXR, A. oncocalyx and onco A treatments. In conclusion, under our culture conditions, A. oncocalyx and onco A do not exhibit a toxic effect on isolated secondary follicles and on maturation rates of COCs recovered from antral follicles, however, these drugs negatively affected the COCs viability.  Thus, the use of culture biotechnologies as an in vitro secondary follicle culture and in vitro oocyte maturation toxicity testing are appropriated methods to evaluate the possible effects of drugs in folliculogesis.

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