scholarly journals Survival of human pre-antral follicles after cryopreservation of ovarian tissue, follicular isolation and in vitro culture in a calcium alginate matrix

2008 ◽  
Vol 24 (1) ◽  
pp. 92-99 ◽  
Author(s):  
C. A. Amorim ◽  
A. Van Langendonckt ◽  
A. David ◽  
M.-M. Dolmans ◽  
J. Donnez
Keyword(s):  
In Vitro Culture ◽  
Calcium Alginate ◽  
Ovarian Tissue ◽  
Antral Follicles ◽  
Alginate Matrix

Optimization of medium for in vitro culture of sheep ovarian tissue

2019 ◽  
Vol 171 ◽  
pp. 82-86
Author(s):  
A.S. Seisenbayeva ◽  
V. Isachenko ◽  
Y.A. Assanova ◽  
X.X. Du ◽  
D.Y. Toishybek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Ovarian Tissue

Slush nitrogen vitrification of human ovarian tissue does not alter gene expression and improves follicle health and progression in long-term in vitro culture

2018 ◽  
Vol 110 (7) ◽  
pp. 1356-1366 ◽  
Author(s):  
Vincenza Barbato ◽  
Roberto Gualtieri ◽  
Teresa Capriglione ◽  
Maria Michela Pallotta ◽  
Sabrina Braun ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Culture ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue

In vitro culture of secondary follicles and prematuration of cumulus–oocyte complexes from antral follicles increase the levels of maturation‐related transcripts in bovine oocytes

2019 ◽  
Vol 86 (12) ◽  
pp. 1874-1886
Author(s):  
Francisco Taiã G. Bezerra ◽  
Francisco Edilcarlos O. Lima ◽  
Laís Rayani F. M. Paulino ◽  
Bianca R. Silva ◽  
Anderson W. B. Silva ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Antral Follicles ◽  
Bovine Oocytes

202 EFFECTS OF ASCORBIC ACID ON IN VITRO CULTURE OF CATTLE PREANTRAL FOLLICLES

2010 ◽  
Vol 22 (1) ◽  
pp. 259
Author(s):  
E. R. Andrade ◽  
R. van den Hurk ◽  
L. A. Lisboa ◽  
M. F. Hertel ◽  
F. A. Melo-Sterza ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
In Vitro Culture ◽  
Ovarian Tissue ◽  
Immunohistochemical Analysis ◽  
Development Stage ◽  
Nuclear Antigen ◽  
Primordial Follicles ◽  
Preantral Follicles ◽  
Ovarian Cortex

The mechanisms that regulate the gradual exit of ovarian follicles from the nongrowing, primordial pool are poorly understood. The objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine the effects of this addition on the growth activation and viability of preantral follicles. The ovarian cortex was divided into small fragments; 1 fragment was immediately fixed in Bouin’s solution (control). The other fragments were cultured for 2, 4, 6, or 8 days on culture plates in minimum essential medium (MEM) supplemented with insulin-transferrin-selenium (ITS), pyruvate, glutamine, hypoxantine, BSA, and antibiotics (MEM+) or in MEM+ plus ascorbic acid (5, 25, 50, 100, or 200 μg mL-1). Ovarian tissue was processed for classical histology, TEM, and immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA). Preantral follicles were classified according to their development stage (primordial, intermediate, primary, and secondary) and on the basis of morphological features (normal or degenerated). Pair-wise comparisons were done using Tukey’s procedure. Chi-square test was used to compare percentages of follicles with PCNA-positive granulosa cells. All analyses were done with Statistical Analysis System (SAS Institute, Cary, NC, USA); P ≤ 0.05 was considered significant. Compared with control fragments, the percentage of primordial follicles was reduced (P ≤ 0.05) and the percentage of growing follicles was increased (P ≤ 0.05) in cultured cortical fragments, independent of the tested medium or incubation time. Furthermore, compared with control tissue, culture of ovarian cortex for 8 days reduced the percentages of healthy, viable follicles (P ≤0.05), but not when cultures were supplemented with 25, 50, and 100 μg mL-1 of ascorbic acid. Ultrastructural and immunohistochemical analysis of ovarian cortical fragments cultured for 8 days, however, showed the integrity and viability of follicles only when fragments were cultured in the presence of 50 μg mL-1 of ascorbic acid. In conclusion, this study demonstrated that addition of ascorbic acid to MEM at a concentration of 50 μg mL-1 not only stimulates the activation and subsequent growth of cattle primordial follicles that are cultured in vitro for 8 days but also safeguards the viability of these preantral follicles. E. R. Andrade and A. A. Alfieri are recipients of the PRODOC/CAPES fellowship.

Supportive techniques to investigate in vitro culture and cryopreservation efficiencies of equine ovarian tissue: A review

2020 ◽  
Vol 156 ◽  
pp. 296-309 ◽  
Author(s):  
F.L.N. Aguiar ◽  
G.D.A. Gastal ◽  
K.A. Alves ◽  
B.G. Alves ◽  
J.R. Figueiredo ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Ovarian Tissue

scholarly journals Gene Expression in Granulosa Cells From Small Antral Follicles From Women With or Without Polycystic Ovaries

2019 ◽  
Vol 104 (12) ◽  
pp. 6182-6192 ◽  
Author(s):  
Lisa Ann Owens ◽  
Stine Gry Kristensen ◽  
Avi Lerner ◽  
Georgios Christopoulos ◽  
Stuart Lavery ◽  
...  
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Polycystic Ovary ◽  
Ovarian Tissue ◽  
Polycystic Ovaries ◽  
Vitro Fertilization ◽  
Antral Follicles ◽  
Oocyte Aspiration ◽  
And Function

Abstract Context Polycystic ovary syndrome (PCOS) is the most common cause of anovulation. A key feature of PCOS is arrest of follicles at the small- to medium-sized antral stage. Objective and Design To provide further insight into the mechanism of follicle arrest in PCOS, we profiled (i) gonadotropin receptors; (ii) characteristics of aberrant steroidogenesis; and (iii) expression of anti-Müllerian hormone (AMH) and its receptor in granulosa cells (GCs) from unstimulated, human small antral follicles (hSAFs) and from granulosa lutein cells (GLCs). Setting GCs from hSAFs were collected at the time of cryopreservation of ovarian tissue for fertility preservation and GLCs collected during oocyte aspiration before in vitro fertilization/intracytoplasmic sperm injection. Participants We collected hSAF GCs from 31 women (98 follicles): 10 with polycystic ovaries (PCO) and 21 without. GLCs were collected from 6 women with PCOS and 6 controls undergoing IVF. Main Outcome Measures Expression of the following genes: LHCGR, FSHR, AR, INSR, HSD3B2, CYP11A1, CYP19, STAR, AMH, AMHR2, FST, INHBA, INHBB in GCs and GLCs were compared between women with PCO and controls. Results GCs in hSAFs from women with PCO showed higher expression of LHCGR in a subset (20%) of follicles. Expression of FSHR (P < 0.05), AR (P < 0.05), and CYP11A1 (P < 0.05) was lower, and expression of CYP19A1 (P < 0.05), STAR (P < 0.05), HSD3B2 (P = NS), and INHBA (P < 0.05) was higher in PCO GCs. Gene expression in GL cells differed between women with and without PCOS but also differed from that in GCs. Conclusions Follicle arrest in PCO is characterized in GCs by differential regulation of key genes involved in follicle growth and function.

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