The Changes of Oxidative Status Markers in Patients with Newly Diagnosed Hiv/Aids-Associated Lung`S Tuberculosis after the Course of Treatment

10.12737/5919 ◽  
2014 ◽  
Vol 21 (3) ◽  
pp. 135-139
Author(s):  
Ясинский ◽  
R. Yasinskiy ◽  
Литвиненко ◽  
E. Litvinenko
Keyword(s):  
Oxidative Stress ◽  
Additional Treatment ◽  
Oxidative Status ◽  
The Body ◽  
Newly Diagnosed ◽  
Compensatory Mechanisms ◽  
Stress Markers ◽  
Pathogenetic Therapy ◽  
Protein Peroxidation ◽  
Hiv Aids

The changes in oxidative status indicators in patients with newly diagnosed HIV/AIDS-associated pulmonary tuberculosis after the treatment depending on the course of the therapy were evaluated. It was found the increasing in the levels of protein peroxidation markers in spontaneous and induced oxidation and of lipid peroxidation markers in patients who treated with standard anti-TB and antiretroviral therapy in the dynamics. Its indicates the rise of the oxidative stress severity and disruption of adaptive-compensatory mechanisms, that exacerbates the disease. There were decreased levels of oxidative stress markers in dynamics and in comparison with indicators of other patients after 3 months of treatment in patients, who received the additional treatment with additional pathogenetic therapy with the inclusion of Contrycal, Glutoxim and laser therapy. The antioxidant protection indicators in patients, who received the additional pathogenetic treatment returned to normal or increased in the dynamics and these levels were higher, than that of other patients. In patients, treated with standard therapy, most indicators decreased in dynamics and in comparison with rates in control patients, and patients from the1 group. This suggests an imbalance in the oxidative status in the body after the standard treatment appointment and necessity of its pathogenetic correction.

scholarly journals Oxidative Stress in BPH

2009 ◽  
Vol 48 (173) ◽  
Author(s):  
Murat Savas ◽  
A Verit ◽  
H Ciftci ◽  
E Ciftci ◽  
E Aktan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Statistical Significance ◽  
Previous Treatment ◽  
Oxidative Status ◽  
Prostatic Hyperplasia ◽  
Newly Diagnosed ◽  
Antioxidative Status ◽  
Total Antioxidant ◽  
And Control ◽  
The Relationship

INTRODUCTION:In the present study, we investigated the relationship between potency of oxidative stress and BPH and this may assist to contribute to the realistic explanation of the ethiopathogenesis of BPH.METHODS:Seventy four newly diagnosed men with BPH (mean age: 54+/-11.2), who had not undergone any previous treatment for BPH, and 62 healthy volunteers (mean age: 55+/-14) were enrolled in the present study. To determine the antioxidative status of plasma, total antioxidant capacity (TAC) was calculated, and to determine the oxidative status of plasma (TOS) total peroxide levels were measured. The ratio of TAC to total peroxide was accepted as an indicator of oxidative stress (OSI). Data are presented as mean SD +/- unless specified. Student t-test and correlation analyses were used to evaluate the statistical significance differences in the median values recorded for all parameters between BPH and control group.RESULTS:Plasma TAC TOS were found in patients and controls (1.70 +/- 0.32, 1.68 +/- 0.19 micromol Trolox Equiv./L), (12.48 +/- 1.98, 12.40 +/- 1.14 micromol / L) respectively. OSI was calculated as 7.57 +/- 1.91, 7.48 +/- 1.33, respectively. Plasma TAC, TOS and OSI levels were not found to be significantly difference between patients and control subjects (p>0.05, p>0.05, p>0.05).CONCLUSIONS:The present study has shown that there were not relationship between potency of oxidative stress and BPH. Further well designed studies should be planned to find out whether the oxidative stress-related parameters play role in BPH as an interesting pathology in regard of the etiopathogenesis.Keywords: benign prostatic hyperplasia, oxidative stress, prostate

scholarly journals Association of vitamin-D deficiency with oxidative stress in newly diagnosed type 2 diabetes

2017 ◽  
Vol 5 (12) ◽  
pp. 5221
Author(s):  
Bairapogu John Pradeep Kumar ◽  
Maliyannar Itaggappa ◽  
Kamandalagondi Vasudevareddy Thimmaraju
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Oxidative Stress Markers ◽  
Newly Diagnosed ◽  
Stress Markers ◽  
Type 2 Diabetics ◽  
Vitamin D Levels

Background: According to a recent World Health Organization (WHO) report, India, with 32 million diabetic individuals, currently has the highest incidence of diabetes worldwide; these numbers are predicted to increase to 80 million by the year 2030. Deficiency of vitamin D has been associated with increased risk of developing Type 2 diabetes mellitus (DM) and cardiovascular diseases. Vitamin D deficiency is highly prevalent in our country. About 70% of adults in both rural and urban areas were found showing manifestations of vitamin D deficiency. Therefore, we designed this study to assess the vitamin D status of the study population by measuring serum 25(OH) D levels, and its association with oxidative stress markers in type 2 diabetes mellitus.Methods: This is a cross sectional study with Group 1 (n=147): Newly diagnosed type 2 diabetics and Group 2 (n=147): Apparently healthy individuals. Blood was collected by venipuncture. 5ml of blood was collected and allowed to clot. Serum was separated and stored in refrigerator to estimate the oxidative stress markers and vitamin D levels. Estimation of vitamin D levels and oxidative stress markers were carried out by commercially available kits.Results: Vitamin D levels are significantly low in newly diagnosed type 2 diabetics when compared to controls (p < 0.000), whereas the FBG levels are significantly high in newly diagnosed type 2 diabetics when compared to controls (p < 0.000). The TAOS levels are significantly low in newly diagnosed type 2 diabetics when compared to controls (p <0.000), whereas the MDA levels are significantly high in newly diagnosed type 2 diabetics when compared to controls (p < 0.000). The oxidative stress marker TAOS (r = 0.71; p < 0.000), was positively correlated and MDA (r = - 0.85; p < 0.000), was negatively with Vitamin D in newly diagnosed type 2 diabetics.Conclusions: From this study, it is concluded that, lower levels of vitamin D is associated with increased oxidative stress. Therapeutic interventions to increase the vitamin D levels and reduce the oxidative stress should be included as a part of treatment inn newly diagnosed type diabetics.

scholarly journals Inflammatory and oxidative status in European captive black rhinoceroses: a link with Iron Overload Disorder?

2020 ◽  
Author(s):  
Hanae Pouillevet ◽  
Nicolas Soetart ◽  
Delphine Boucher ◽  
Rudy Wedlarski ◽  
Laetitia Jaillardon
Keyword(s):  
Oxidative Stress ◽  
Iron Overload ◽  
Serum Iron ◽  
Iron Status ◽  
The Body ◽  
Stress Markers ◽  
Oxidative Markers ◽  
Inflammatory Status ◽  
The Impact ◽  
And Oxidative Stress

AbstractIron Overload Disorder (IOD) is a syndrome developed by captive browsing rhinoceroses like black rhinoceroses (Diceros bicornis) in which hemosiderosis settles in vital organs while free iron accumulates in the body, potentially predisposing to various secondary diseases. Captive grazing species like white rhinoceroses (Ceratotherium simum) do not seem to be affected. The pro-oxidant and pro-inflammatory properties of iron, associated with the poor antioxidant capacities of black rhinoceroses, could enhance high levels of inflammation and oxidative stress leading to rapid ageing and promoting diseases. In this prospective study, 15 black (BR) and 29 white rhinoceroses (WR) originating from 22 European zoos were blood-sampled and compared for their iron status (serum iron), liver/muscle biochemical parameters (AST, GGT, cholesterol), inflammatory status (total proteins, protein electrophoresis) and oxidative stress markers (SOD, GPX, dROMs). Results showed higher serum iron and liver enzyme levels in black rhinoceroses (P<0.01), as well as higher GPX (P<0.05) and dROM (P<0.01) levels. The albumin/globulin ratio was lower in black rhinoceroses (P<0.05) due to higher α2-globulin levels (P<0.001). The present study suggests a higher inflammatory and oxidative profile in captive BR than in WR, possibly in relation to iron status. This could be either a consequence or a cause of iron accumulation, potentially explaining rapid ageing and various diseases. Further investigations are needed to assess the prognostic value of the inflammatory and oxidative markers in captive black rhinoceroses, particularly for evaluating the impact of reduced-iron and antioxidant-supplemented diets.

scholarly journals Markers of Oxidative Stress and Antioxidant Status in the Plasma, Urine and Saliva of Healthy Mice

2018 ◽  
pp. 921-934 ◽  
Author(s):  
M. GYURÁSZOVÁ ◽  
A. KOVALČÍKOVÁ ◽  
K. JANŠÁKOVÁ ◽  
K. ŠEBEKOVÁ ◽  
P. CELEC ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Blood Collection ◽  
Reference Ranges ◽  
Oxidative Stress Markers ◽  
Male Adult ◽  
Antioxidant Power ◽  
Stress Markers ◽  
Status Assessment

Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, healthy mice and to identify some sources of variability. Samples were obtained from 41 female and 37 male adult, healthy mice of the CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges of TBARS (thiobarbituric acid reactive substances), AOPP (advanced oxidation protein products), fructosamine, GSH/GSSG (reduced and oxidized glutathione) ratio, TAC (total antioxidant capacity), and FRAP (ferric reducing antioxidant power) were measured in plasma and urine, and TBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standard spectrophotometric and fluorometric methods. Salivary GSH/GSSG and urinary AOPP were higher in females. Urinary fructosamine, GSH/GSSG and FRAP were higher in males. Urinary TAC and FRAP negatively correlated with age, and urinary GSH/GSSG positively correlated with weight. We determined that urine and saliva can be obtained non-invasively from mice, in sufficient amounts for reliable oxidative status assessment. Further studies are needed to uncover whether these biofluids reflect systemic oxidative status in diseases.

scholarly journals Oxidative Stress Markers to Investigate the Effects of Hyperoxia in Anesthesia

2019 ◽  
Vol 20 (21) ◽  
pp. 5492 ◽  
Author(s):  
Ottolenghi ◽  
Rubino ◽  
Sabbatini ◽  
Coppola ◽  
Veronese ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
The Body ◽  
Antioxidant Defenses ◽  
Oxidative Stress Biomarkers ◽  
Stress Markers ◽  
Baseline Values ◽  
Fraction Of Inspired Oxygen ◽  
Student’S T ◽  
Inspired Oxygen ◽  
Student’S T Test

Oxygen (O2) is commonly used in clinical practice to prevent or treat hypoxia, but if used in excess (hyperoxia), it may act as toxic. O2 toxicity arises from the enhanced formation of Reactive Oxygen Species (ROS) that exceed the antioxidant defenses and generate oxidative stress. In this study, we aimed at assessing whether an elevated fraction of inspired oxygen (FiO2) during and after general anesthesia may contribute to the unbalancing of the pro-oxidant/antioxidant equilibrium. We measured five oxidative stress biomarkers in blood samples from patients undergoing elective abdominal surgery, randomly assigned to FiO2 = 0.40 vs. 0.80: hydroperoxides, antioxidants, nitrates and nitrites (NOx), malondialdehyde (MDA), and glutathionyl hemoglobin (HbSSG). The MDA concentration was significantly higher 24 h after surgery, and the body antioxidant defense lower, in the FiO2 = 0.80 group with respect to both the FiO2 = 0.40 group and the baseline values (p ≤ 0.05, Student’s t-test). HbSSG in red blood cells was also higher in the FiO2 = 0.80 group at the end of the surgery. NOx was higher in the FiO2 = 0.80 group than the FiO2 = 0.40 group at t = 2 h after surgery. MDA, the main end product of the peroxidation of polyunsaturated fatty acids directly influenced by FiO2, may represent the best marker to assess the pro-oxidant/antioxidant equilibrium after surgery.

scholarly journals Hepatoprotective Effect of Citrus limon Fruit Extract against Carbofuran Induced Toxicity in Wistar Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Sunil Kumar Jaiswal ◽  
Vivek K. Gupta ◽  
Nikhat J. Siddiqi ◽  
Ravi S. Pandey ◽  
Bechan Sharma
Keyword(s):  
Oxidative Stress ◽  
Acetylcholinesterase Activity ◽  
The Body ◽  
Citrus Limon ◽  
Fruit Extract ◽  
Stress Markers ◽  
Pesticide Stress ◽  
Ameliorative Effect ◽  
Redox Molecules ◽  
Hepatic Markers

Carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranol methylcarbamate), is known to induce oxidative stress and to cause inhibition of acetylcholinesterase activity. The present work was envisaged to evaluate the effect of carbofuran on redox indices and its interactions with hepatic markers in rat. The ameliorating effect of Citrus limon fruit extract on carbofuran induced toxicity was also monitored. The results indicated that carbofuran treatment caused significant alterations in the levels of activities of AST, ALT, and LDH in liver tissues and serum. The levels of enzymatic oxidative stress markers such as SOD and catalase and nonenzymatic redox molecules such as total thiol, GSH, and protein thiol also showed significant perturbations in rat liver due to carbofuran treatment. The administration of Citrus limon fruit extract, however, was able to markedly ameliorate the toxicity of carbofuran by protecting the levels of aforesaid biomarkers to near normal levels. The ameliorative effect of Citrus limon fruit extract may be due to the presence of different antioxidants in it which may neutralize the ROS and RNS generated in the body tissue due to pesticide stress. These results suggested that Citrus limon fruit extract may be utilized as a potential supplement in proper management of pesticide intoxication in association with relevant therapeutics.

scholarly journals Effects of aqueous extract of polyherbal formulation against hyperthyroidism induced by L-thyroxin in a rat model

2018 ◽  
Vol 5 (12) ◽  
pp. 2876-2887 ◽  
Author(s):  
Sahar B. Ahmed ◽  
Asmaa M. Moghazy ◽  
Omar A. Ahmed-Farid ◽  
Hassan A. Esebery
Keyword(s):  
Oxidative Stress ◽  
Thyroid Hormone ◽  
Low Dose ◽  
Colorimetric Method ◽  
The Body ◽  
Male Rats ◽  
High Dose ◽  
Phytochemical Analysis ◽  
Oxidative Stress Markers ◽  
Stress Markers

Background: Hyperthyroidism is a disorder that occurs when the thyroid gland secretes more thyroid hormone than the body needs. Thyroid hormone is essential for the normal growth and development of normal organs. Polyherb (POH) formulation has proven to be useful in number of diseases and has been used in folk medicine as an anti-hyperthyroidism, anti-oxidant, and appetitestimulating agent. The aim of the study was to evaluate the curative effect of POH against L-thyroxin (LT4)-induced hyperthyroidism in male rats. Methods: Seven groups (10 rats each) were used for this purpose. Determination of phytochemical analysis, oxidative stress markers, brain appetite marker and cell energy marker were determined via high-performance liquid chromatography (HPLC) techniques. Thyroid hormones were detected via ELISA, and liver functions were determined by colorimetric method. Results: The data showed that LT4 altered thyroid function via decreasing serum Thyroid-stimulating hormone (TSH), serum total protein, albumin and globulin, while increasing Triiodothyronine (T3), Thyroxine (T4), and Aspartate aminotransferase (AST). Moreover, oxidative stress markers in liver tissues were increased, via up-regulation of nitric oxide (NO), oxidized glutathione (GSSG), malondialdehyde (MDA), and 8-hydroxy-2'-deoxyguanosine (8OHdG). Meanwhile, glutathione (GSH) and ATP were alleviated; in contrast, metabolites of ADP and AMP were elevated. Neuronal appetite marker in brain tissue was decreased via low serotonin levels. On the other hand, rat groups treated with POH and Carbimazole (CBZ) showed markedly amelioration of hyperthyroidism in rats at low dose only but did not show complete amelioration at high dose of POH. The data were confirmed through histopathological examination of the thyroid. Conclusion: The data obtained demonstrated that POH, at low dose, can be very effective for completely treating hyperthyroidism in rats, and was safer than Carbimazole (CBZ) and ameliorated most signaling pathways and in different tissues.

scholarly journals Is the Oxidative Stress Really a Disease?

2016 ◽  
Vol 62 (1) ◽  
pp. 112-120 ◽  
Author(s):  
Erzsébet Fogarasi ◽  
Mircea Dumitru Croitoru ◽  
Ibolya Fülöp ◽  
Daniela-Lucia Muntean
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
The Body ◽  
Myeloperoxidase Activity ◽  
Stress Markers ◽  
Tyrosine Derivatives ◽  
Radical Generation ◽  
Endogenous Antioxidant ◽  
Derivatives Of ◽  
Biomarkers Of Oxidative Stress

AbstractOxidative stress is an imbalance between free radicals or other reactive species and the antioxidant activity of the organism. Oxidative stress can induce several illnesses such as cardiovascular disease, neurodegenerative disorders, diabetes, cancer, Alzheimer and Parkinson. The biomarkers of oxidative stress are used to test oxidative injury of biomolecules. The indicators of lipid peroxidation (malondialdehyde, 4-hydroxy- 2-nonenal, 2-propenal, isoprostanes), of protein oxidation (carbonylated proteins, tyrosine derivatives), of oxidative damage of DNA, and other biomarkers (glutathione level, metallothioneins, myeloperoxidase activity) are the most used oxidative stress markers. Diseases caused by oxidative stress can be prevented with antioxidants. In human body are several enzymes with antioxidant capacity (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase) and spin traps. Antioxidants are synthetized in the organism (glutathione) or arrive in the body by nutrition (ascorbic acid, vitamin E, carotenoids, flavonoids, resveratrol, xanthones). Different therapeutic strategies to reduce oxidative stress with the use of synthetic molecules such as nitrone-based antioxidants (phenyl-α-tert-butyl-nitrone (PBN), 2,4-disulphophenyl- N-tert-butylnitrone (NXY-059), stilbazulenyl nitrone (STAZN), which scavenge a wide variety of free radical species, increase endogenous antioxidant levels and inhibits free radical generation are also tested in animal models.

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