scholarly journals Hepatoprotective Effect of Citrus limon Fruit Extract against Carbofuran Induced Toxicity in Wistar Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Sunil Kumar Jaiswal ◽  
Vivek K. Gupta ◽  
Nikhat J. Siddiqi ◽  
Ravi S. Pandey ◽  
Bechan Sharma
Keyword(s):  
Oxidative Stress ◽  
Acetylcholinesterase Activity ◽  
The Body ◽  
Citrus Limon ◽  
Fruit Extract ◽  
Stress Markers ◽  
Pesticide Stress ◽  
Ameliorative Effect ◽  
Redox Molecules ◽  
Hepatic Markers

Carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranol methylcarbamate), is known to induce oxidative stress and to cause inhibition of acetylcholinesterase activity. The present work was envisaged to evaluate the effect of carbofuran on redox indices and its interactions with hepatic markers in rat. The ameliorating effect of Citrus limon fruit extract on carbofuran induced toxicity was also monitored. The results indicated that carbofuran treatment caused significant alterations in the levels of activities of AST, ALT, and LDH in liver tissues and serum. The levels of enzymatic oxidative stress markers such as SOD and catalase and nonenzymatic redox molecules such as total thiol, GSH, and protein thiol also showed significant perturbations in rat liver due to carbofuran treatment. The administration of Citrus limon fruit extract, however, was able to markedly ameliorate the toxicity of carbofuran by protecting the levels of aforesaid biomarkers to near normal levels. The ameliorative effect of Citrus limon fruit extract may be due to the presence of different antioxidants in it which may neutralize the ROS and RNS generated in the body tissue due to pesticide stress. These results suggested that Citrus limon fruit extract may be utilized as a potential supplement in proper management of pesticide intoxication in association with relevant therapeutics.

Ameliorative Effect of Grewia tenax (Forssk) Fiori Fruit Extract on CCl4-Induced Oxidative Stress and Hepatotoxicity in Rats

2011 ◽  
Vol 76 (9) ◽  
pp. T200-T206 ◽  
Author(s):  
Mansour S. Al-Said ◽  
Ramzi A. Mothana ◽  
Mohammed O. Al-Sohaibani ◽  
Syed Rafatullah
Keyword(s):  
Oxidative Stress ◽  
Fruit Extract ◽  
Ameliorative Effect ◽  
Grewia Tenax

scholarly journals Glycyrrhizic acid ameliorates submandibular gland oxidative stress, autophagy and vascular dysfunction in rat model of type 1 diabetes

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Saad Mohamed Asseri ◽  
Nehal M. Elsherbiny ◽  
Mohamed El-Sherbiny ◽  
Iman O. Sherif ◽  
Alsamman M. Alsamman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Model ◽  
Glycyrrhizic Acid ◽  
Type I Diabetes ◽  
Vascular Dysfunction ◽  
Type I ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Ameliorative Effect ◽  
Related Proteins

AbstractThe burden of diabetes mellitus (DM) and associated complications is increasing worldwide, affecting many organ functionalities including submandibular glands (SMG). The present study aims to investigate the potential ameliorative effect of glycyrrhizic acid (GA) on diabetes-induced SMG damage. Experimental evaluation of GA treatment was conducted on a rat model of type I diabetes. Animals were assigned to three groups; control, diabetic and GA treated diabetic groups. After 8 weeks, the SMG was processed for assessment of oxidative stress markers, autophagy related proteins; LC3, Beclin-1 and P62, vascular regulator ET-1, aquaporins (AQPs 1.4 and 5), SIRT1 protein expressions in addition to LC3 and AQP5 mRNA expressions. Also, parenchymal structures of the SMG were examined. GA alleviated the diabetes-induced SMG damage via restoring the SMG levels of oxidative stress markers and ET-1 almost near to the normal levels most probably via regulation of SIRT1, AQPs and accordingly LC-3, P62 and Beclin-1levels. GA could be a promising candidate for the treatment of diabetes-induced SMG damage via regulating oxidative stress, autophagy and angiogenesis.

The Changes of Oxidative Status Markers in Patients with Newly Diagnosed Hiv/Aids-Associated Lung`S Tuberculosis after the Course of Treatment

10.12737/5919 ◽  
2014 ◽  
Vol 21 (3) ◽  
pp. 135-139
Author(s):  
Ясинский ◽  
R. Yasinskiy ◽  
Литвиненко ◽  
E. Litvinenko
Keyword(s):  
Oxidative Stress ◽  
Additional Treatment ◽  
Oxidative Status ◽  
The Body ◽  
Newly Diagnosed ◽  
Compensatory Mechanisms ◽  
Stress Markers ◽  
Pathogenetic Therapy ◽  
Protein Peroxidation ◽  
Hiv Aids

The changes in oxidative status indicators in patients with newly diagnosed HIV/AIDS-associated pulmonary tuberculosis after the treatment depending on the course of the therapy were evaluated. It was found the increasing in the levels of protein peroxidation markers in spontaneous and induced oxidation and of lipid peroxidation markers in patients who treated with standard anti-TB and antiretroviral therapy in the dynamics. Its indicates the rise of the oxidative stress severity and disruption of adaptive-compensatory mechanisms, that exacerbates the disease. There were decreased levels of oxidative stress markers in dynamics and in comparison with indicators of other patients after 3 months of treatment in patients, who received the additional treatment with additional pathogenetic therapy with the inclusion of Contrycal, Glutoxim and laser therapy. The antioxidant protection indicators in patients, who received the additional pathogenetic treatment returned to normal or increased in the dynamics and these levels were higher, than that of other patients. In patients, treated with standard therapy, most indicators decreased in dynamics and in comparison with rates in control patients, and patients from the1 group. This suggests an imbalance in the oxidative status in the body after the standard treatment appointment and necessity of its pathogenetic correction.

scholarly journals Inflammatory and oxidative status in European captive black rhinoceroses: a link with Iron Overload Disorder?

2020 ◽  
Author(s):  
Hanae Pouillevet ◽  
Nicolas Soetart ◽  
Delphine Boucher ◽  
Rudy Wedlarski ◽  
Laetitia Jaillardon
Keyword(s):  
Oxidative Stress ◽  
Iron Overload ◽  
Serum Iron ◽  
Iron Status ◽  
The Body ◽  
Stress Markers ◽  
Oxidative Markers ◽  
Inflammatory Status ◽  
The Impact ◽  
And Oxidative Stress

AbstractIron Overload Disorder (IOD) is a syndrome developed by captive browsing rhinoceroses like black rhinoceroses (Diceros bicornis) in which hemosiderosis settles in vital organs while free iron accumulates in the body, potentially predisposing to various secondary diseases. Captive grazing species like white rhinoceroses (Ceratotherium simum) do not seem to be affected. The pro-oxidant and pro-inflammatory properties of iron, associated with the poor antioxidant capacities of black rhinoceroses, could enhance high levels of inflammation and oxidative stress leading to rapid ageing and promoting diseases. In this prospective study, 15 black (BR) and 29 white rhinoceroses (WR) originating from 22 European zoos were blood-sampled and compared for their iron status (serum iron), liver/muscle biochemical parameters (AST, GGT, cholesterol), inflammatory status (total proteins, protein electrophoresis) and oxidative stress markers (SOD, GPX, dROMs). Results showed higher serum iron and liver enzyme levels in black rhinoceroses (P<0.01), as well as higher GPX (P<0.05) and dROM (P<0.01) levels. The albumin/globulin ratio was lower in black rhinoceroses (P<0.05) due to higher α2-globulin levels (P<0.001). The present study suggests a higher inflammatory and oxidative profile in captive BR than in WR, possibly in relation to iron status. This could be either a consequence or a cause of iron accumulation, potentially explaining rapid ageing and various diseases. Further investigations are needed to assess the prognostic value of the inflammatory and oxidative markers in captive black rhinoceroses, particularly for evaluating the impact of reduced-iron and antioxidant-supplemented diets.

PERBEDAAN UJI AKTIVITAS ANTIOKSIDAN ANTARA EKSTRAK BUAH KIWI DAN APEL SECARA IN VITRO

2020 ◽  
Vol 10 (1) ◽  
pp. 90
Author(s):  
Nur Ariska Nugrahani
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Natural Antioxidants ◽  
Apple Fruit ◽  
The Body ◽  
Fruit Extract ◽  
Kiwi Fruit ◽  
Dpph Method

Radikal bebas yang berbahaya jika konsentrasi ROS akan meningkat sehingga akan mengakibatkan stress oksidatif. Stress oksidatif biasanya disebabkan oleh konsentrasi tinggi dari radikal bebas dalam sel dan jaringan yang dapat diinduksi oleh beberapa faktor negatif seperti gamma, UV, radiasi sinar-X, tekanan psiko-emosional dan makanan yang tercemar.Untuk meminimalisir radikal bebas dalam tubuh diperlukan antioksidan alami seperti ekstrak buah kiwi dan ekstrak buah apel. Uji aktivitas antioksidan pada masing- masing ekstrak dilakukan dengan metode DPPH (1,1- difenil-2-pikrihidazil). Hasil menunjukkan bahwa nilai IC50 dari ekstrak buah kiwi lebih rendah daripada IC50 ekstrak buah apel. Hal ini menunjukkan bahwa ekstrak buah kiwi mempunyai aktivitas antioksidan lebih kuat dari ekstrak buah apel. Keyword : Radikal bebas, DPPH, Ekstrak buah kiwi, Ekstrak buah apel Free radicals will be dangerous if the ROS concentration will increase and causes oxidative stress. Oxidative stress is usually caused by high concentrations of free radicals in cells and tissues which can be induced by several negative factors such as gamma, UV, X-ray radiation, psycho-emotional pressure and contaminated food. To minimize free radicals in the body natural antioxidants such as kiwi fruit extract and apple extract. The antioxidant activity test on each extract was carried out by the DPPH method (1,1-diphenyl-2-picrihydazyl). The results showed that IC50 value of kiwi fruit extract was lower than IC50 of apple fruit extract. This shows that kiwi fruit extract has stronger antioxidant activity than apple extract. Keyword : free radicals, DPPH, kiwi fruit extract, apple fruit extract

scholarly journals Oxidative Stress Markers to Investigate the Effects of Hyperoxia in Anesthesia

2019 ◽  
Vol 20 (21) ◽  
pp. 5492 ◽  
Author(s):  
Ottolenghi ◽  
Rubino ◽  
Sabbatini ◽  
Coppola ◽  
Veronese ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
The Body ◽  
Antioxidant Defenses ◽  
Oxidative Stress Biomarkers ◽  
Stress Markers ◽  
Baseline Values ◽  
Fraction Of Inspired Oxygen ◽  
Student’S T ◽  
Inspired Oxygen ◽  
Student’S T Test

Oxygen (O2) is commonly used in clinical practice to prevent or treat hypoxia, but if used in excess (hyperoxia), it may act as toxic. O2 toxicity arises from the enhanced formation of Reactive Oxygen Species (ROS) that exceed the antioxidant defenses and generate oxidative stress. In this study, we aimed at assessing whether an elevated fraction of inspired oxygen (FiO2) during and after general anesthesia may contribute to the unbalancing of the pro-oxidant/antioxidant equilibrium. We measured five oxidative stress biomarkers in blood samples from patients undergoing elective abdominal surgery, randomly assigned to FiO2 = 0.40 vs. 0.80: hydroperoxides, antioxidants, nitrates and nitrites (NOx), malondialdehyde (MDA), and glutathionyl hemoglobin (HbSSG). The MDA concentration was significantly higher 24 h after surgery, and the body antioxidant defense lower, in the FiO2 = 0.80 group with respect to both the FiO2 = 0.40 group and the baseline values (p ≤ 0.05, Student’s t-test). HbSSG in red blood cells was also higher in the FiO2 = 0.80 group at the end of the surgery. NOx was higher in the FiO2 = 0.80 group than the FiO2 = 0.40 group at t = 2 h after surgery. MDA, the main end product of the peroxidation of polyunsaturated fatty acids directly influenced by FiO2, may represent the best marker to assess the pro-oxidant/antioxidant equilibrium after surgery.

scholarly journals Effects of aqueous extract of polyherbal formulation against hyperthyroidism induced by L-thyroxin in a rat model

2018 ◽  
Vol 5 (12) ◽  
pp. 2876-2887 ◽  
Author(s):  
Sahar B. Ahmed ◽  
Asmaa M. Moghazy ◽  
Omar A. Ahmed-Farid ◽  
Hassan A. Esebery
Keyword(s):  
Oxidative Stress ◽  
Thyroid Hormone ◽  
Low Dose ◽  
Colorimetric Method ◽  
The Body ◽  
Male Rats ◽  
High Dose ◽  
Phytochemical Analysis ◽  
Oxidative Stress Markers ◽  
Stress Markers

Background: Hyperthyroidism is a disorder that occurs when the thyroid gland secretes more thyroid hormone than the body needs. Thyroid hormone is essential for the normal growth and development of normal organs. Polyherb (POH) formulation has proven to be useful in number of diseases and has been used in folk medicine as an anti-hyperthyroidism, anti-oxidant, and appetitestimulating agent. The aim of the study was to evaluate the curative effect of POH against L-thyroxin (LT4)-induced hyperthyroidism in male rats. Methods: Seven groups (10 rats each) were used for this purpose. Determination of phytochemical analysis, oxidative stress markers, brain appetite marker and cell energy marker were determined via high-performance liquid chromatography (HPLC) techniques. Thyroid hormones were detected via ELISA, and liver functions were determined by colorimetric method. Results: The data showed that LT4 altered thyroid function via decreasing serum Thyroid-stimulating hormone (TSH), serum total protein, albumin and globulin, while increasing Triiodothyronine (T3), Thyroxine (T4), and Aspartate aminotransferase (AST). Moreover, oxidative stress markers in liver tissues were increased, via up-regulation of nitric oxide (NO), oxidized glutathione (GSSG), malondialdehyde (MDA), and 8-hydroxy-2'-deoxyguanosine (8OHdG). Meanwhile, glutathione (GSH) and ATP were alleviated; in contrast, metabolites of ADP and AMP were elevated. Neuronal appetite marker in brain tissue was decreased via low serotonin levels. On the other hand, rat groups treated with POH and Carbimazole (CBZ) showed markedly amelioration of hyperthyroidism in rats at low dose only but did not show complete amelioration at high dose of POH. The data were confirmed through histopathological examination of the thyroid. Conclusion: The data obtained demonstrated that POH, at low dose, can be very effective for completely treating hyperthyroidism in rats, and was safer than Carbimazole (CBZ) and ameliorated most signaling pathways and in different tissues.

scholarly journals Is the Oxidative Stress Really a Disease?

2016 ◽  
Vol 62 (1) ◽  
pp. 112-120 ◽  
Author(s):  
Erzsébet Fogarasi ◽  
Mircea Dumitru Croitoru ◽  
Ibolya Fülöp ◽  
Daniela-Lucia Muntean
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
The Body ◽  
Myeloperoxidase Activity ◽  
Stress Markers ◽  
Tyrosine Derivatives ◽  
Radical Generation ◽  
Endogenous Antioxidant ◽  
Derivatives Of ◽  
Biomarkers Of Oxidative Stress

AbstractOxidative stress is an imbalance between free radicals or other reactive species and the antioxidant activity of the organism. Oxidative stress can induce several illnesses such as cardiovascular disease, neurodegenerative disorders, diabetes, cancer, Alzheimer and Parkinson. The biomarkers of oxidative stress are used to test oxidative injury of biomolecules. The indicators of lipid peroxidation (malondialdehyde, 4-hydroxy- 2-nonenal, 2-propenal, isoprostanes), of protein oxidation (carbonylated proteins, tyrosine derivatives), of oxidative damage of DNA, and other biomarkers (glutathione level, metallothioneins, myeloperoxidase activity) are the most used oxidative stress markers. Diseases caused by oxidative stress can be prevented with antioxidants. In human body are several enzymes with antioxidant capacity (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase) and spin traps. Antioxidants are synthetized in the organism (glutathione) or arrive in the body by nutrition (ascorbic acid, vitamin E, carotenoids, flavonoids, resveratrol, xanthones). Different therapeutic strategies to reduce oxidative stress with the use of synthetic molecules such as nitrone-based antioxidants (phenyl-α-tert-butyl-nitrone (PBN), 2,4-disulphophenyl- N-tert-butylnitrone (NXY-059), stilbazulenyl nitrone (STAZN), which scavenge a wide variety of free radical species, increase endogenous antioxidant levels and inhibits free radical generation are also tested in animal models.

scholarly journals Protective assessment of cimetidine against cyclophosphamide-induced kidney injury

2018 ◽  
Vol 9 (6) ◽  
pp. 25-30
Author(s):  
Elias Adikwu ◽  
Bonsome Bokolo
Keyword(s):  
Oxidative Stress ◽  
Kidney Injury ◽  
The Body ◽  
The Other ◽  
Oxidative Stress Markers ◽  
Medical Sciences ◽  
Adult Rats ◽  
Sodium Potassium ◽  
Stress Markers ◽  
Kidney Functions

Background: Nephrotoxicity is one of the frequent toxicities observed with cyclophosphamide (CP) use which may involve oxidative stress. Cimetidine is an antihistamine with anti-oxidative stress activity.Aims and Objectives: The study aimed to evaluate the effect of cimetidine on cyclophosphamide-induced kidney damage in albino rats.Materials and Methods: Forty eight adult rats randomised into 8 (A-H) groups of 6 rats per group were experimentally used for this study.Group A (control) was treated with water, while groups B-D were treated with 5, 10 and 20 mg/kg of cimetidine intraperitoneally (ip) daily for 5 days respectively. Group E was treated with150 mg/kgof CP ip on the 5th day. Groups F-H were pretreated with 5, 10 and 20 mg/kg cimetidine ip daily for 5 days and treated with CP ip on the 5th day respectively. Rats were sacrificed serum was extracted from blood and evaluated for renal function markers, while kidneys were harvested and evaluated for oxidative stress markers and histology.Results: There were no significant effects (p>0.05) on the body and kidney weights of CP-treated rats. However, impaired kidney functions in CP-treated rats were marked by significant (p<0.05) increases in creatinine, urea, uric acid, sodium, potassium, chloride, bicarbonate, and malondialdehyde levels when compared to control. On the other hand, significant (p<0.05) decreases in superoxide dismutase, catalase, glutathione, glutathione peroxidase, total protein and albumin were obtained in CP-treated rats when compared to control. Necrotic changes were observed in the kidneys of CP-treated rats. However, CP-induced nephrotoxic effects were significantly (p<0.05; 0.01) reversed in cimetidine pretreated rats.Conclusion: Cimetidine shows potential as adjunct remedy for cyclophosphamide associated nephrotoxicity.Asian Journal of Medical Sciences Vol.9(6) 2018 25-30

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