Violation of the Patterns of Distribution of Hela Cells on the Substrate during Low-Frequency Pulsed Electromagnetic Radiation Effect (15 HZ)

10.12737/5890 ◽  
2014 ◽  
Vol 21 (3) ◽  
pp. 18-22
Author(s):  
Омельчук ◽  
N. Omelchuk ◽  
Бунин ◽  
K. Bunin ◽  
Симаков ◽  
...  
Keyword(s):  
Electromagnetic Radiation ◽  
Cancer Cells ◽  
Hela Cells ◽  
Human Cancer ◽  
Low Frequency ◽  
Malignant Cells ◽  
Pulsed Field ◽  
Cells In Culture ◽  
Cell Line Hela ◽  
Pulsed Electromagnetic

The experiment was carried out on the cell line HeLa, which is one of the most famous among researchers, biologists and physicians; it is widely used in laboratories to identify factors that inhibit the cancerous growth, as well as for testing and testing of various drugs. It is shown that after 60 minutes of exposure field electromagnetic with a frequency of 15 Hz. from impulse wave of the device "Kamena" in the culture of cancer cells occurs morphological structural adjustment. HeLa cells are compressed and reduced contact with the substrate and form becomes elongated and wedge-shaped. In addition, under the influence of pulsed field electromagnetic with a frequency of 15 Hz patterns (patterns), formed by HeLa cells resembling a "flower", dissolved, and cells randomly appears in the culture. HeLa cells, after exposure to the field of apparatus "Kameny, become different dimension that allows to speak about the development of anisocytosis, which indicates a decrease in the resistance of cancer cells in culture for them to adverse factors. During the same time period in the control (without effects of pulsed EMR) is observed only move in HeLa cells relatively dark marks due to horizontal migration. This compression cells and destruction of patterns that originally marked in culture, in the form of the "flower", didn’t observed. Revealed the effect of low-frequency field electromagnetic on the morphology of cancer cells and on the patterns of their distribution on the substrate may be important for therapy of malignant tumors. This experiment doesn’t yet allow by the authors to answer the question: how long the effect on human cancer cells can be maintained in culture and how safe the use of this pulsed field EMR to inhibit the activity of malignant cells. In these experiments, the authors observed an increase in cell proliferation in culture of malignant cells. All of this suggests that further research of revealed effect of low-intensity pulsed electromagnetic radiation on malignant cells in culture is necessary.

scholarly journals Copper(ii) quinolinonato-7-carboxamido complexes as potent antitumor agents with broad spectra and selective effects

RSC Advances ◽  
2016 ◽  
Vol 6 (5) ◽  
pp. 3899-3909 ◽  
Author(s):  
Radka Křikavová ◽  
Ján Vančo ◽  
Zdeněk Trávníček ◽  
Roman Buchtík ◽  
Zdeněk Dvořák
Keyword(s):  
Cancer Cells ◽  
Antitumor Agents ◽  
Human Cancer ◽  
Malignant Cells ◽  
Lower Toxicity ◽  
Human Cancer Cells ◽  
Selective Effects

[Cu(quix)(phen)]NO3·yH2O (quix = 2-(4-amino-3,5-dichlorophenyl)-3-hydroxy-4(1H)-quinolinone-7-carboxamides) showed potent cytotoxicity against human cancer cells, lower toxicity on non-malignant cells, and ability to interact with biomolecules.

scholarly journals Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential

2020 ◽  
Vol 12 (1) ◽  
pp. 1-11
Author(s):  
Efraín A Cermeño ◽  
Meghan J O’Melia ◽  
Woojin M Han ◽  
Austin Veith ◽  
Graham Barber ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Adhesion Strength ◽  
Human Cancer ◽  
Low Frequency ◽  
Label Free ◽  
Tumor Initiating Cells ◽  
Adhesive Properties ◽  
Human Cancer Cells ◽  
Tumorigenic Potential ◽  
Cancerous Cells

Abstract Tumor-initiating cells (TICs), a subpopulation of cancerous cells with high tumorigenic potential and stem-cell-like properties, drive tumor progression and are resistant to conventional therapies. Identification and isolation of TICs are limited by their low frequency and lack of robust markers. Here, we characterize the heterogeneous adhesive properties of a panel of human and murine cancer cells and demonstrate differences in adhesion strength among cells, which exhibit TIC properties and those that do not. These differences in adhesion strength were exploited to rapidly (~10 min) and efficiently isolate cancerous cells with increased tumorigenic potential in a label-free manner by use of a microfluidic technology. Isolated murine and human cancer cells gave rise to larger tumors with increased growth rate and higher frequency in both immunocompetent and immunocompromised mice, respectively. This rapid and label-free TIC isolation technology has the potential to be a valuable tool for facilitating research into TIC biology and the development of more efficient diagnostics and cancer therapies.

scholarly journals Leunca (Solanum nigrum L.) Herbs Ethanolic Extract Increase Cytotoxic Activity of Cisplatin on Hela Cervical Cancer Cells

2010 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Raditya Prima Istiaji ◽  
Maya Fitria ◽  
Larasati Larasati ◽  
Fortunella Tjondro ◽  
Astrid Ayu Maruti ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Side Effects ◽  
Cancer Cells ◽  
Hela Cells ◽  
Human Cancer ◽  
Ethanolic Extract ◽  
Solanum Nigrum ◽  
Human Cancer Cells ◽  
Cervical Cancer Cells ◽  
Solanum Nigrum L

Cervical cancer is one of leading causes of cancer death in women in the developing countries. The use of cisplatin as chemotherapy agent in cervical cancer is known to cause side effects and also resistance for long-term uses. One of the strategies to prevent cervical cancer based on combination agents is being developed. Leunca (Solanum nigrum L.) has been revealed to inhibit growth of human cancer cells. Therefore, it can be used in combination with cisplatin to reduce those side effects and prevent the occurrence of cell resistance. Ethanolic extract of Leunca Herb (ELH) and cisplatin were tested their cytotoxic effect on HeLa cervical cancer cell by using MTT assay to determine IC50 value. The combinationss of cisplatin-ELH were tested to determine the combination index (CI value). The IC50 of ELH and cisplatin on HeLa cells were 227 µg/mL and 17 µM. rRespectively. Tthe study of combination resulted that almost all the index combinations were <0,9 showed the effect of synergism combination. The Ooptimum concentration of combination was 1/8 IC50 cisplatin–1/8 IC50 ELH. The results indicated that ELH had a potency to be combination agent to enhance the activity of cisplatin on HeLa cervical cancer cells. Therefore, further study on its molecular mechanism needs to be explored. Key words: Leunca (Solanum nigrum L.), cisplatin, cytotoxic, combination agent, HeLa cells

scholarly journals The Potential of 9,10-Anthraquinone in Inhibiting Human Cancer Cells Growth

2021 ◽  
Vol 15 (1) ◽  
pp. 19
Author(s):  
Irmanida Batubara ◽  
Arif Rakhman Hakim ◽  
Silmi Mariya ◽  
Suminar Setiati Achmadi ◽  
Valentina Sokoastri Valentina Sokoastri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Growth ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Hela Cells ◽  
Human Cancer ◽  
Cancer Cell Growth ◽  
Human Cancer Cells ◽  
Hct 116 ◽  
Mcf 7

Background: 9,10-Anthraquinone (9,10-AQ) is a contaminant on some agricultural products and considered as carcinogenic based on EU Regulation No. 1146/2014. Except for little evidence on experimental rats, there is no strong proof regarding the carcinogenicity in humans. Therefore, it is essential to find a safe dose of this compound since the difference in 9,10-AQ levels will affect cancer cell growth. This research aims to find the 9,10-AQ concentration that does not proliferate the human cancer cells under in vitro study.Methods: In determining the 9,10-AQ concentration that does not proliferate the cancer cells growth, 0.01 to 500 mg/L 9,10-AQ was directly tested on four human cancer cells (colorectal carcinoma HCT 116, colon adenocarcinoma WiDr, breast cancer MCF-7, and cervical cancer HeLa), and the viability of the cells was counted via (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. In the gene expression level, the effects on a selected cancer cell line were determined by qRT-PCR against BAX, BCL-2, PCNA, and P53.Results: The result indicates that 9,10-AQ up to 500 mg/L concentration does not proliferate the cell’s growth but instead inhibits those four cancer cells’ growths. The concentration of 9,10-AQ that inhibits 50% the cancer cells growth (IC50) value was 321.8 mg/L (1.55 mM) against HCT 116 and above 500 mg/L (above 2.40 mM) against WiDr, MCF-7, and HeLa. The 9,10-AQ at 500 mg/L (or 2.40 mM) increases BAX expression and acts as an apoptotic agent on HeLa cells.Conclusions: The investigation has shown that 9,10-AQ up to 500 mg/L concentration does not proliferate the cancer cell growth; instead, it inhibits the HCT 116 and HeLa cells growth. We have preliminary evidence regarding the apoptotic mechanism of 9,10-AQ by increasing BAX gene expression on HeLa cells.

scholarly journals Auto-catalysed progression of aneuploidy explains the Hayflick limit of cultured cells, carcinogen-induced tumours in mice, and the age distribution of human cancer

2000 ◽  
Vol 348 (3) ◽  
pp. 497-506 ◽  
Author(s):  
David RASNICK
Keyword(s):  
Cancer Cells ◽  
Time Course ◽  
Cultured Cells ◽  
Human Cancer ◽  
Age Distribution ◽  
Spontaneous Transformation ◽  
Cells In Culture ◽  
Hayflick Limit ◽  
Modelling Studies ◽  
Diploid Cells

Evidence continues to accumulate that aneuploidy, an imbalance in the number of chromosomes, is responsible for the characteristic phenotypes of cancer, including the abnormal cellular size and morphology of cancer cells, the appearance of tumour-associated antigens, as well as the high levels of membrane-bound and secreted proteins responsible for invasiveness and loss of contact inhibition. Aneuploidy has also been demonstrated to be the self-perpetuating source of the karyotypic instability of cancer cells. Here it is shown that the auto-catalysed progression of aneuploidy explains the kinetics of the finite lifetime of diploid cells in culture, the time course of the appearance of papillomas and carcinomas in benzo[a]pyrene-treated mice, and the age-dependence of human cancers. Modelling studies indicate that the ease of spontaneous transformation of mouse cells in culture may be due to a chaotic progression of aneuploidy. Conversely, the strong preference towards senescence and resistance to transformation of human cells in culture may be the result of a non-chaotic progression of aneuploidy. Finally, a method is proposed for quantifying the aneuploidogenic potencies of carcinogens.

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