scholarly journals BABESIA INFECTION OF SMALL MAMMALS FROM SOUTHERN TAIGA OF OMSK REGION

2016 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Боброва ◽  
Oksana Bobrova ◽  
Танцев ◽  
Aleksey Tantsev ◽  
Епихина ◽  
...  
Keyword(s):  
Small Mammals ◽  
Small Mammal ◽  
Nested Pcr ◽  
Southern Taiga ◽  
Babesia Microti ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Genetic Groups ◽  
Tamias Sibiricus ◽  
Babesia Spp

Blood samples were taken from 541 small mammal captured in 2013–2015 in Znamensky district of Omsk region from Ixodes persulcatus and Ixodes trianguliceps sympatric area and examined for the Babesia spp. presence by nested PCR with subsequent sequencing of positive samples. Babesia microti DNA was found in 31,1 % of positive samples; a proportion of infected mammals varied from 5,3 % to 61,6 % in different sampling periods. B. microti DNA was found in samples from three prevailing Myodes species as well as from a root vole (Microtus oeconomus), field voles (Microtus argestis) and Siberian chipmunks (Tamias sibiricus). It was shown that identified B. microti samples belong to two genetic groups: B. microti ‘US’-type and B. microti ‘Munich’-type; notably that > 90 % infected mammals contained DNA of nonpathogenic for human B. microti ‘Munich’-type. We suppose that I. trianguliceps tick is the most probable vector of B. microti ‘Munich’-type.

scholarly journals Выявление различных видов риккетсий у иксодовых клещей, в крови людей и мелких млекопитающих на юге Западной Сибири и на Урале

2006 ◽  
Vol 5 ◽  
pp. 121-125
Author(s):  
Ya. P. Igolkina ◽  
N. V. Fomenko ◽  
N. N. Livanova ◽  
V. B. Astanin ◽  
L. A. Gosteeva ◽  
...  
Keyword(s):  
Small Mammals ◽  
Human Blood ◽  
Nested Pcr ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Rickettsia Helvetica ◽  
Rickettsia Slovaca ◽  
High Prevalence ◽  
Human Blood Samples ◽  
Rickettsia Sibirica

We investigated ticks, human blood samples, specimens of tissue and blood from small mammals using nested PCR. We de- tected high prevalence Rickettsia tarasevichiae and Rickettsia sp. RpA4 in samples of ticks Ixodes persulcatus and Dermacentor re- ticulatus, correspondingly. We observed DNA of pathogenic Rickettsia helvetica and Rickettsia slovaca in I. persulcatus and Der- macentor marginatus and DNA of Rickettsia sibirica in human blood and in blood and liver samples of small mammals.

scholarly journals The study of anaplasma and ehrlichia genetic variability in parasitic systems in the South-Western Siberia and Ural

2006 ◽  
Vol 5 ◽  
pp. 116-120
Author(s):  
V. A. Rar ◽  
N. N. Livanova ◽  
V. V. Panov ◽  
V. B. Astanin ◽  
S. G. Livanov ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Small Mammals ◽  
Genetic Variants ◽  
Nested Pcr ◽  
Anaplasma Phagocytophilum ◽  
Western Siberia ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Novosibirsk Region ◽  
Ehrlichia Muris

Ehrlichia muris and Anaplasma phagocytophilum DNA were detected using nested PCR among Ixodes persulcatus collected in Novosibirsk, Sverdlovsk and Chelyabinsk regions and in blood samples of small mammals from Novosibirsk and Sverdlovsk re- gions. Two genetic variants of A. phagocytophilum were revealed in blood samples and ticks from Novosibirsk region.

scholarly journals Blood parasites in dogs from the Belgrade area in the period 2014-2015

2017 ◽  
pp. 69-78
Author(s):  
Ivan Pavlovic ◽  
Ivana Kovacevic ◽  
Radoje Doder ◽  
Branislav Rangelov ◽  
Ivana Nikic ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Rhipicephalus Sanguineus ◽  
Blood Parasites ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Test Kit ◽  
Blood Smears ◽  
Shelter Dogs ◽  
First Time ◽  
Babesia Spp

Prevalence of blood parasites in dogs in the Belgrade area has been investigated continuously during the last 20 years, especially in clinically suspicious dogs. In the period from 2014 to 2015, 249 blood samples of pets (182) and shelter dogs (67) were examined. Using Giemsa-stained blood smears, the presence of Babesia spp. was examined in erythrocytes and the presence of morulae of Ehrlichia spp. and Anaplasma spp. in circu?lating monocytes and granulocytes. To confirm positive findings of ehrlichiosis and ana?plasmosis in blood smears, CaniV-4 Test Kit or IDEXX SNAP 4DX test was used. Infection with two pathogens was found in 78/249 (31.32%) cases; in all cases, the infection with one of the protozoa or bacteria was in combination with heartworms. In blood-smears, babesiosis was found in 39.75% of pet dogs and in 71.64% of shelter dogs, ehrlichiosis in 15.93% and 28.35%, and anaplasmosis in 6.04% and 19.40%, respectivelly. From colected ticks, relative abundance analysis revealed that the species Ixodes ricinus was absolutely dominant and found in 50.53% (47/93), followed by Rhipicephalus sanguineus ? 38.70% (36/93), Derma?centor marginatus ? 9.67% (9/93), D. reticulatus, and Ixodes persulcatus found in 3.22% (3/93), which for the first time occurred in dogs in the Belgrade area and in Serbia.

Genetic diversity ofBabesiainIxodes persulcatusand small mammals from North Ural and West Siberia, Russia

Parasitology ◽  
2010 ◽  
Vol 138 (2) ◽  
pp. 175-182 ◽  
Author(s):  
V. A. RAR ◽  
T. I. EPIKHINA ◽  
N. N. LIVANOVA ◽  
V. V. PANOV
Keyword(s):  
Genetic Diversity ◽  
Small Mammals ◽  
West Siberia ◽  
18S Rrna Gene ◽  
Sensu Stricto ◽  
Babesia Microti ◽  
Rrna Gene ◽  
Ixodes Persulcatus ◽  
Novosibirsk Region ◽  
The Us

SUMMARYObjective.The aim of this work was to study the prevalence and genetic diversity ofBabesiainIxodes persulcatusticks and small mammals from Ural and Siberia in Russia.Methods.In total, 481 small mammals and 922 questing adultI. persulcatusfrom North Ural (Sverdlovsk region) and West Siberia (Novosibirsk region) were examined for the presence ofBabesiaby nested PCR based on the 18S rRNA gene.Results.Babesia microtiof the ‘Munich’-type was found in 36·2% of blood samples of the small mammals from the Sverdlovsk region andB. microtiof the ‘US’-type in 5·3% of the animals from the Novosibirsk region.BabesiaDNA was not detected in 133 analysedI. persulcatusfrom the Sverdlovsk region; however, it was found in 24 of 789 ticks from the Novosibirsk region. Three distinctBabesiaspecies were detected inI. persulcatus. B. microti‘US’-type was identified in 10 ticks,Babesiaclosely related toB. divergens/B. capreoliin 2 ticks, andBabesiaclosely related toB. venatorum(EU1) in 12 ticks.Conclusion.To our knowledge, this is the first detection ofBabesia sensu strictoinI. persulcatusticks and ofB. microtiinI. persulcatusin the Asian part of Russia.

scholarly journals Prevalence of Orthohantavirus-Reactive Antibodies in Humans and Peri-Domestic Rodents in Northern Ethiopia

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1054
Author(s):  
Yonas Meheretu ◽  
Åsa Granberg ◽  
Gebregiorgis Berhane ◽  
Hussein Khalil ◽  
Olivia Wesula Lwande ◽  
...  
Keyword(s):  
Small Mammals ◽  
Small Mammal ◽  
Immunofluorescence Assay ◽  
Northern Ethiopia ◽  
Natural Habitats ◽  
Blood Samples ◽  
Virus Rna ◽  
Close Proximity ◽  
Seasonal Infection ◽  
Low Prevalence

In 2012, Tigray orthohantavirus was discovered in Ethiopia, but its seasonal infection in small mammals, and whether it poses a risk to humans was unknown. The occurrence of small mammals, rodents and shrews, in human inhabitations in northern Ethiopia is affected by season and presence of stone bunds. We sampled small mammals in two seasons from low- and high-density stone bund fields adjacent to houses and community-protected semi-natural habitats in Atsbi and Hagere Selam, where Tigray orthohantavirus was first discovered. We collected blood samples from both small mammals and residents using filter paper. The presence of orthohantavirus-reactive antibodies in blood was then analyzed using immunofluorescence assay (human samples) and enzyme linked immunosorbent assays (small mammal samples) with Puumala orthohantavirus as antigen. Viral RNA was detected by RT-PCR using small mammal blood samples. Total orthohantavirus prevalence (antibodies or virus RNA) in the small mammals was 3.37%. The positive animals were three Stenocephalemys albipes rats (prevalence in this species = 13.04%). The low prevalence made it impossible to determine whether season and stone bunds were associated with orthohantavirus prevalence in the small mammals. In humans, we report the first detection of orthohantavirus-reactive IgG antibodies in Ethiopia (seroprevalence = 5.26%). S. albipes lives in close proximity to humans, likely increasing the risk of zoonotic transmission.

High prevalence of Babesia microti ‘Munich’ type in small mammals from an Ixodes persulcatus/Ixodes trianguliceps sympatric area in the Omsk region, Russia

2016 ◽  
Vol 115 (9) ◽  
pp. 3619-3629 ◽  
Author(s):  
Vera Rar ◽  
Valeriy Yakimenko ◽  
Marat Makenov ◽  
Artem Tikunov ◽  
Tamara Epikhina ◽  
...  
Keyword(s):  
Small Mammals ◽  
Babesia Microti ◽  
Ixodes Persulcatus ◽  
High Prevalence ◽  
Ixodes Trianguliceps

scholarly journals DETECTION OF BABESIA AND ANAPLASMATACEAE BACTERIA IN SMALL MAMMALS FROM IRKUTSK AND NOVOSIBIRSK REGIONS

2016 ◽  
Vol 1 (3) ◽  
pp. 37-41
Author(s):  
Козлова ◽  
Irina Kozlova ◽  
Лисак ◽  
Oksana Lisak ◽  
Панов ◽  
...  
Keyword(s):  
Small Mammals ◽  
Babesia Microti ◽  
Ixodes Persulcatus ◽  
Small Rodents ◽  
Tissue Samples ◽  
Candidatus Neoehrlichia Mikurensis ◽  
Novosibirsk Region ◽  
Irkutsk Region ◽  
Pcr Method ◽  
Neoehrlichia Mikurensis

Tissue samples from 228 small mammals captured from 2013 to 2015 in four districts of Irkutsk region and in one district of Novosibirsk region were examined for the presence of Anaplasmataceae bacteria and Babesia parasites by nested PCR method with subsequent sequencing of positive samples. In Ekhirit-Bulagatskiy District of Irkutsk region, Babesia microti DNA was found in 10.9 % of small mammals, Anaplasma phagocytophilum DNA – in 7.3 %, and Ehrlichia muris DNA – in 1.8 %. Infected mammals were not found in the other three examined districts of Irkutsk region. In Novosibirsk region, B. microti DNA was found in 8.9 % of small rodents, A. phagocytophilum DNA – in 8.9 % of small mammals, and Candidatus Neoehrlichia mikurensis DNA – in 8.9 % of small mammals. The identified infection agents corresponded to microorganism species and genetic variants previously found in Ixodes persulcatus ticks but not in ticks of other species.

scholarly journals Molecular characteristics of HBV infection among blood donors tested HBsAg reactive in a single ELISA test in southern China

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xianlin Ye ◽  
Tong Li ◽  
Ran Li ◽  
Heng Liu ◽  
Junpeng Zhao ◽  
...  
Keyword(s):  
Viral Load ◽  
Hepatitis B ◽  
Nested Pcr ◽  
Blood Donors ◽  
Southern China ◽  
Core Promoter ◽  
Elisa Test ◽  
Hbv Infection ◽  
Molecular Characteristics ◽  
Blood Samples

Abstract Background Hepatitis B virus (HBV) infection is a major concern for blood safety in high-prevalence HBV countries such as China. In Shenzhen, dual hepatitis B surface antigen (HBsAg) enzyme-linked immunosorbent assays (ELISAs) have been adopted in parallel with nucleic acid testing (NAT) for donors for over a decade. A small proportion of blood donors test reactive (R) for HBsAg but negative through routine NAT, which can lead to HBV infection with an extremely low viral load. Objectives We aimed to investigate and analyze the molecular characteristics of HBV among blood donors that tested HBsAg R in a single ELISA test. Methods Blood donations were evaluated in this study if confirmed HBsAg R through one of two ELISA kits. Samples with non-reactive (NR) results by NAT were collected and tested for HBsAg by chemiluminescent microparticle immunoassay (CLIA) with a neutralization test. The level of HBsAg was further assessed by electrochemiluminescence immunoassay (ECLIA). The viral basic core promoter (BCP) and pre-core (PC) and S regions were amplified by nested PCR. Quantitative real-time PCR (qPCR) for viral load determination and individual donation (ID)-NAT were adopted simultaneously. HBsAg was confirmed with CLIA, ECLIA, nested PCR, qPCR, and ID-NAT. Results Of the 100,252 donations, 38 and 41 were identified as HBsAg R with Wantai and DiaSorin ELISA kits, respectively. Seventy-nine (0.077%, 79/100,252) blood samples with ELISA R-NR and NAT NR results were enrolled in the study. Of these, 17 (21.5%,17/79) were confirmed as HBsAg-positive. Of the 14 genotyped cases, 78.6% (11/14) were genotype B, and C and D were observed in two and one sample, respectively. Mutations were found in the S gene, including Y100C, Y103I, G145R, and L175S, which can affect the detection of HBsAg. A high-frequency mutation, T1719G (93.3%), was detected in the BCP/PC region, which reduced the viral replication. Conclusion A small number of blood samples with HBsAg ELISA R-NR and NAT NR results were confirmed as HBV infection, viral nucleic acids were found in most of the samples through routine NAT methods. It is necessary to employ more sensitive and specific assays for the detection of HBV infection among blood donors.

Understory use by terrestrial small mammals in an unflooded forest patch in the Pantanal floodplain

Mammalia ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nayara Yoshie Sano ◽  
Heitor Miraglia Herrera ◽  
Grasiela Edith de Oliveira Porfirio ◽  
Filipe Martins Santos
Keyword(s):  
Small Mammals ◽  
Small Mammal ◽  
Understory Vegetation ◽  
Monodelphis Domestica ◽  
Forest Patch ◽  
Habitat Features ◽  
Species Characteristics ◽  
Local Habitat

AbstractTo date, there have been no studies that have evaluated small mammal utilization of the understory of forests. In this study, we described the use of vertical strata by small mammals in patches of unflooded forests, known as “cordilheiras”, in the Nhecolândia sub-region of the Pantanal, Brazil. We collected all species using the ground and understory, including the terrestrial didelphid Monodelphis domestica. We suppose that local habitat features (e.g., Acuri palms), rather than intrinsic species characteristics, may be more conducive to the use of understory vegetation by small mammals in the Nhecolândia region.

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