DYNAMICS OF INDICES OF LINOLEIC AND ARACHIDONIC FATTY ACIDS IN CORD BLOOD OF NEWBORNS FROM MOTHERS WITH CYTOMEGALOVIRUS INFECTION

2016 ◽  
Vol 1 (60) ◽  
pp. 75-79
Author(s):  
Дорофиенко ◽  
Nikolay Dorofienko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cytomegalovirus Infection ◽  
Umbilical Artery ◽  
Control Group ◽  
Gas Liquid Chromatography ◽  
Igg Antibodies

Due to the broad spectrum of influence of polyunsaturated fatty acids of ω-6 family on the development of the fetus and newborn child, the composition of arachidonic fatty acid and its predecessor linoleic fatty acid were studied in blood of the umbilical cord blood of newborns from mothers with chronic reactivation of cytomegalovirus infection. 82 newborns were examined; among them there were 42 infants (the main group) from mothers who had cytomegalovirus infection (titer of IgG antibodies to cytomegalovirus was 1:1600) in the III trimester. The control group included 42 newborns from healthy women. In the umbilical cord blood of newborns the contents of arachidonic and linoleic fatty acids were identified with gas-liquid chromatography. The study found out that the reactivation of cytomegalovirus infection in the mother (titer of IgG antibodies to cytomegalovirus 1:1600) leads to an increase in the content of linoleic acid by 56% (p<0.001) and of the predecessor of pro-inflammatory eicosanoids - arachidonic acid by 34% (p<0.001) in the cord blood of newborns; it is one of the causes of disturbance of the structural and functional organization of the umbilical artery vessels. Identified changes in umbilical cord blood and umbilical artery endothelium can cause disturbances of the elastic properties of vessels, stagnation and thrombosis of blood vessels and, consequently, disturbances of blood flow between mother and fetus, which can then serve as a factor in the development of fetal hypoxia.

Acute maternal cytomegalovirus infection is associated with significantly decreased numbers of CD34+ cells in umbilical cord blood

2012 ◽  
Vol 49 (3-4) ◽  
pp. 166-169 ◽  
Author(s):  
José C. Jaime-Pérez ◽  
Julia E. Colunga-Pedraza ◽  
Roberto Monreal-Robles ◽  
Perla R. Colunga-Pedraza ◽  
Nereida Méndez-Ramírez ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cytomegalovirus Infection ◽  
Cd34 Cells

scholarly journals Associations of maternal prenatal dietary intake of n-3 and n-6 fatty acids with maternal and umbilical cord blood levels

2009 ◽  
Vol 80 (5-6) ◽  
pp. 289-296 ◽  
Author(s):  
S.M.A. Donahue ◽  
S.L. Rifas-Shiman ◽  
S.F. Olsen ◽  
D.R. Gold ◽  
M.W. Gillman ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dietary Intake ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Levels

scholarly journals The content of gas transmitters in umbilical cord blood and blood of newborns born to mothers with preeclampsia

2021 ◽  
Vol 66 (4) ◽  
pp. 53-57
Author(s):  
I. G. Popova ◽  
O. G. Sitnikova ◽  
S. B. Nazarov ◽  
G. N. Kuzmenko ◽  
M. M. Klychevа ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Life Support ◽  
Venous Blood ◽  
The Body ◽  
Control Group ◽  
Control Groups ◽  
And Control

To assess endothelial function in the newborns born to mothers with preeclampsia and without preeclampsia the scientists assessed the content of nitricoxide and hydrogen sulfide in the umbilical and venous blood of these children. The main group consisted of 80 women with moderate preeclampsia and their80 newborns; the control group included 50 children born to women without preeclampsia. The authors studied the content of nitric oxide by determining the totalnitrates and nitrites (NOx), hydrogen sulfide in mixed cord blood collected within 30 minutes after delivery, and venous blood taken from newborns on the 1-3rd day of life. Umbilical cord blood demonstrated no significant differences in the content of NO xand hydrogen sulfide in newborns of the main and control groups. However, in general, the study of umbilical cord blood revealed a relationship between the concentration of hydrogen sulfide and the possibility of developing intraventricular hemorrhagein a newborn of the main and control groups. In the venous blood there was a significant increase in the content of NOx and hydrogen sulfide in newborns born to mothers with preeclampsia. It may cause vasodilation asa compensatory reaction of the body during the period of postnatal adaptation of the child’s body, aimed at life support.

scholarly journals Gestational Diabetes Mellitus in Pregnancy Increased Erythropoietin Level Affecting Differentiation Potency of Haematopoietic Stem Cell of Umbilical Cord Blood

2021 ◽  
Vol 8 ◽  
Author(s):  
Mohd Razif Mohd Idris ◽  
Fazlina Nordin ◽  
Zaleha Abdullah Mahdy ◽  
S. Fadilah Abd Wahid
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cell ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
In Utero ◽  
Haematopoietic Stem Cell ◽  
Control Group

Background: The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and haematopoietic stem cell (HSC) quality. The aim of this study is to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC in terms of differentiation potency including the UCB parameters used for banking and transplantation purposes.Methods: UCB-HSC was collected from 42 GDM and 38 normal pregnancies. UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme linked immunoassay (ELISA) technique.Result: The UCB parameters; volume, total nucleated count (TNC) and total CD34+ cells were significantly reduced in the GDM group compared to the control group. The number of HSC progenitors' colonies were significantly reduced in the GDM group except for progenitor BFU-E, which was significantly increased (GDM = 94.19 ± 6.21, Control = 73.61 ± 2.73, p = 0.010). This data was associated with higher EPO level in GDM group. However, the insulin level in the GDM group was comparable to the Control group.Conclusion: Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy such as GDM might affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

scholarly journals Is low birth weight a risk indicator for congenital cytomegalovirus infection?

2009 ◽  
Vol 4 (01) ◽  
pp. 044-047 ◽  
Author(s):  
Zakyieh Al-Hareth ◽  
Fawza Monem ◽  
Nagwa Abdel Megiud
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Head Circumference ◽  
Cytomegalovirus Infection ◽  
Congenital Infection ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus

Background: Congenital cytomegalovirus infection is currently the leading cause of congenital infection in 0.2-2.2% of live births worldwide leading to variable serious sequalae. The aim of the study was to determine if low birth weight is an indicator of CMV congenital infection evidenced by detecting CMV-DNA in umbilical cord blood at the time of delivery. Methodology: CMV-IgG and IgM antibodies and CMV-DNAemia were assessed in umbilical cord blood of two hundreds newborns, one hundred of whom had birth weight ≤ 2700 gram and/or head circumference ≤ 32 cm. Results: CMV-IgM was not detected, while CMV-IgG was positive in 80-90% of the two hundreds tested newborns. CMV-DNA was detected in four out of the 200 newborns. One of them was over the adopted weight limit (> 2700 gram). Conclusions: CMV-IgM and IgG antibodies assessment was not a potential discriminative test to identify congenitally infected newborns. In addition, low birth weight and small head circumference at birth failed to predict congenital CMV infection. CMV-DNA detection in umbilical cord blood at the time of delivery using real-time PCR of all newborns is recommended as decisive, rapid and non-invasive test.

scholarly journals Gestational diabetes mellitus in pregnancy increased erythropoietin level affecting differentiation potency of haematopoietic stem cell of umbilical cord blood

2020 ◽  
Author(s):  
Mohd Razif Mohd Idris ◽  
Fazlina Nordin ◽  
Zaleha Abdullah Mahdy ◽  
S Fadilah Abd Wahid
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cell ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
In Utero ◽  
Haematopoietic Stem Cell ◽  
Control Group

Abstract Background The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines, and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and quality of haematopoietic stem cell (HSC). This study aims to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC regarding its differentiation potency, including the UCB parameters used for banking and transplantation purposes. Methods UCB-HSC was collected from 42 GDM and 38 normal pregnancies. UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme-linked immunoassay (ELISA) technique. Results The UCB parameters, i.e., volume, total nucleated count (TNC), and total CD34+ cells, were significantly reduced in the GDM group compared to the control group. The number of HSC progenitor colonies was significantly reduced in the GDM group, except for progenitor BFU-E, which was significantly increased (GDM = 94.19 ± 6.21, Control = 73.61 ± 2.73, p = 0.010). This data was associated with a higher EPO level in the GDM group. However, the insulin level in the GDM group was comparable to the control group. Conclusion Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy, such as GDM might affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

scholarly journals The Influence of Gestational Diabetes Mellitus upon the Selected Parameters of the Maternal and Fetal System of Insulin-Like Growth Factors (IGF-1, IGF-2, IGFBP1-3)—A Review and a Clinical Study

2020 ◽  
Vol 9 (10) ◽  
pp. 3256
Author(s):  
Tomasz Gęca ◽  
Anna Kwaśniewska
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Peripheral Blood ◽  
Control Group ◽  
Study Group ◽  
Igfbp 3 ◽  
Insulin Like Growth Factors

Background: Gestational diabetes mellitus (GDM), defined as impaired glucose tolerance with onset or first recognition in pregnancy, increases the risk of not only maternal but also fetal and neonatal complications. Given the structural similarity of insulin-like growth factors with insulin and participation of components of the insulin-like growth factor system in glucose homeostasis, we hypothesized that the IGF axis is involved in the development of GDM complications or its pathogenesis. The aim of this study was to evaluate the effect of GDM on the selected parameters of the insulin-like growth factors (IGF-1, IGF-2, IGFBP1-3) in the maternal and fetal blood. Methods: The clinical material of this case-control study included 109 pregnant women and their offspring. The study group (n = 120) consisted of 60 patients with diagnosed gestational diabetes and their newborn babies. The control group (n = 98) comprised 49 healthy parturients and their offspring. We measured the concentrations of IGF-1, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, insulin and glucose made by the ELISA method in peripheral blood serum in patients suffering from GDM and pregnant women without GDM, and in the umbilical cord blood of newborn babies born to them. Results: The analysis of concentrations of IGF-1, -2 and IGFBP-3 in peripheral blood as well as umbilical cord blood did not demonstrate a statistically significant difference between the study group and the control group. Significantly lower concentration of IGFBP-1, IGFBP-2 in peripheral blood and in umbilical cord blood was detected in the study group in comparison to the control group. A statistically positive correlation between the concentration of IGF-1 in umbilical cord serum of newborn babies born to women with gestational diabetes and the length of a baby after its birth was observed. Conclusions: Gestational diabetes mellitus does not significantly affect the concentrations of IGF-1, -2, IGFBP-3 in the peripheral blood and umbilical cord blood, but has the greatest influence on maternal and fetal IGFBP-2 concentrations. A positive correlation between the concentration of IGF-1 in umbilical cord blood and the length of a newborn suggests an influence of IGF-1 on the process of fetal development.

Use of Human Umbilical Cord Serum to Treat Animal Skin Burns

2019 ◽  
Vol 28 (2) ◽  
pp. 165-173
Author(s):  
Hanan J. Kassab ◽  
Maysoon A. MERDAW ◽  
Intesar J. Al- Ramahi ◽  
Alaa A. Alasadi ◽  
Sawsan Al Mousauy ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Burn Injury ◽  
Healing Process ◽  
Boiling Water ◽  
Control Group ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Hair Regrowth

Objective: The objective of the study was to test the hypothesis, that umbilical cord blood crude serum applied topically with its high concentration of growth factors may promote an early healing for animal models with burn injury. Methods:  Fresh human umbilical cord blood UCBS was collected and screened for transmitted diseases such as hepatitis B, hepatitis C and HIV. Mice and rabbits were scalded by boiling water and chemical burning by NaOH, then treated with UCBS. In comparison with a Cetrimide treated group and a control group (without treatment). The UCBS was subjected to microbial testing to demonstrate the presence or absence of extraneous viable contaminating microorganisms. Results: Topical application of UCBS promoted the healing process; complete healing was seen after 10 days for the mice group and in 7 days in the rabbit group. Cetrimide group applied to the rabbit showed slow healing and needed 10 days for hair regrowth. Control group in both mice and rabbits showed very slow response and the burn area diameter remained the same for over 10 days, and no hair regrowth was obvious after 10 days.    Conclusion: The results of the current study indicated that UCBS is a promising therapy for the healing of burns by boiling water and/or chemical substance, more clinical trials are needed to further explore the long-term effects after UCBS use.

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