scholarly journals Studies on physiologically active substances in citrus fruit peel. Part XX. Structure and physiological activity of phenyl propanoid glycosides in lemon (Citrus limon Burm. f.) peel.

1991 ◽  
Vol 55 (3) ◽  
pp. 647-650 ◽  
Author(s):  
Yoshiharu MATSUBARA ◽  
Takehiko YUSA ◽  
Akiyoshi SAWABE ◽  
Yoshitomi IIZUKA ◽  
Kozo OKAMOTO
Keyword(s):  
Physiological Activity ◽  
Citrus Fruit ◽  
Citrus Limon ◽  
Fruit Peel ◽  
Phenyl Propanoid ◽  
Physiologically Active Substances ◽  
Active Substances

The Content of Physiologically Active Substances in Several Medicinal Mushrooms with Various Doses of γ-Irradiation

2014 ◽  
Vol 936 ◽  
pp. 707-711
Author(s):  
Shih Jeng Huang ◽  
Ya Ling Liu ◽  
Jeng Leun Mau ◽  
Shu Yao Tsai
Keyword(s):  
Gamma Irradiation ◽  
Physiological Activity ◽  
Hot Water ◽  
Aminobutyric Acid ◽  
Antrodia Camphorata ◽  
Γ Irradiation ◽  
Medicinal Mushrooms ◽  
Physiologically Active Substances ◽  
Γ Rays ◽  
Active Substances

Dry samples of fruit bodies of Cordyceps militaris and Agariucs blazei, mycelia of Antrodia camphorata, and hot water extracts of Ganoderma tsugae were ground and irradiated with γ-rays at doses of 0, 2.5, 5, and 10 kGy. After γ-irradiation, the content of γ-aminobutyric acid, adenosine, cordycepin, and triterpenoids in medicinal mushrooms did not significantly change. Gamma-irradiation only affects A. camphorata mycelia polysaccharide content, and an increase in irradiation dose (0-10 kGy) significantly reduces its content (111.85-101.88 mg/g). The content of high molecular weight (2.7×105 Da) polysaccharide in G. tsugae by γ-irradiation with increasing doses gradually increased. Gamma-irradiation could significantly affect the content of vitamin D2 and ergosterol. However, ergosterol content in non-irradiated C. militaris, A. blazei, A. camphorata, and G. tsugae was 2.34, 1.31, 2.33, and 1.15 mg/g, and of that in 10 kGy γ-irradiated mushrooms was 2.75, 1.88, 2.19, and 0.94 mg/g, respectively. Summarily, γ-irradiation does change some medicinal mushrooms by the content of physiologically active substances. When using γ-irradiation sterilization to maintain the physiological activity of mushroom products, one should consider its impact on the physiologically active substances.

scholarly journals Recent Advances in Quenchbody, a Fluorescent Immunosensor

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1223
Author(s):  
Jinhua Dong ◽  
Hiroshi Ueda
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescent Dyes ◽  
Antibody Fragment ◽  
Disease Diagnosis ◽  
Disease Biomarkers ◽  
Highly Sensitive ◽  
Physiologically Active Substances ◽  
New Type ◽  
Antigen Antibody ◽  
Active Substances

The detection of viruses, disease biomarkers, physiologically active substances, drugs, and chemicals is of great significance in many areas of our lives. Immunodetection technology is based on the specificity and affinity of antigen–antibody reactions. Compared with other analytical methods such as liquid chromatography coupled with mass spectrometry, which requires a large and expensive instrument, immunodetection has the advantages of simplicity and good selectivity and is thus widely used in disease diagnosis and food/environmental monitoring. Quenchbody (Q-body), a new type of fluorescent immunosensor, is an antibody fragment labeled with fluorescent dyes. When the Q-body binds to its antigen, the fluorescence intensity increases. The detection of antigens by changes in fluorescence intensity is simple, easy to operate, and highly sensitive. This review comprehensively discusses the principle, construction, application, and current progress related to Q-bodies.

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