scholarly journals Genetic analysis of control of proliferation in fibroblastic cells in culture. Part III. Macromolecular synthesis in a set of temperature-sensitive mutants of rat 3Y1 fibroblasts under proliferation-arresting and proliferation-resuming conditions.

1985 ◽  
Vol 49 (2) ◽  
pp. 381-386 ◽  
Author(s):  
Koji YAMADA ◽  
Genki KIMURA
Keyword(s):  
Genetic Analysis ◽  
Temperature Sensitive ◽  
Macromolecular Synthesis ◽  
Cells In Culture ◽  
Temperature Sensitive Mutants ◽  
Fibroblastic Cells

Isolation and Genetic Analysis of Temperature-Sensitive Mutants of Rhinovirus Type 2

Intervirology ◽  
1980 ◽  
Vol 13 (5) ◽  
pp. 299-306 ◽  
Author(s):  
Martin R. Evans ◽  
John H. Hughes ◽  
Cicek Gercel ◽  
Vincent V. Hamparian
Keyword(s):  
Genetic Analysis ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants

scholarly journals AN ENRICHMENT FOR TEMPERATURE-SENSITIVE MUTANTS IN TETRAHYMENA THERMOPHILA

Genetics ◽  
1979 ◽  
Vol 92 (4) ◽  
pp. 1079-1092
Author(s):  
Duane W Martindale ◽  
Ronald E Pearlman
Keyword(s):  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Wild Type ◽  
Macromolecular Synthesis ◽  
Treatment Results ◽  
Uv Treatment ◽  
Temperature Sensitive Mutants ◽  
Near Ultraviolet ◽  
Treatment Data ◽  
Resistant Mutants

ABSTRACT The parameters for the killing of Tetrahymena by 5-bromodeoxyuridine (BUdR) and near-ultraviolet light have been determined. Significant preferential killing by UV* of cells that have incorporated BUdR was obtained when the cells were irradiated in a nonnutrient buffer. UV alone was found to be toxic to cells irradiated in growth medium. Mutants defective in division at a restrictive temperature were isolated from mutagenized cultures that had been treated with BUdR and UV and from mutagenized cultures that had no such treatment. Results indicate that the number of temperature sensitive (ts) growth mutants can be increased five to six times using the BUdR/UV treatment. Data are presented that indicate differences in the frequency of occurrence of various types of ts mutants, with and without enrichment. A mutant that immediately stopped macromolecular synthesis and cell division upon being placed at the restrictive temperature was more resistant to BUdR/UV treatment than wild type by 1000-fold. Using the above techniques, BUdR-resistant mutants altered in the phosphorylation of thymidine have been isolated.

Genetic analysis of temperature-sensitive mutants of HSV-1: The combined use of complementation and physical mapping for cistron assignment

Virology ◽  
1983 ◽  
Vol 130 (2) ◽  
pp. 290-305 ◽  
Author(s):  
Sandra K. Weller ◽  
Doris P. Aschman ◽  
Wendy R. Sacks ◽  
Donald M. Coen ◽  
Priscilla A. Schaffer
Keyword(s):  
Genetic Analysis ◽  
Physical Mapping ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Combined Use ◽  
Hsv 1
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