Genetic analysis of control of proliferation in fibroblastic cells in culture. I. Isolation and characterization of mutants temperature-sensitive for proliferation or survival of untransformed diploid rat cell line 3Y1

1984 ◽  
Vol 10 (1) ◽  
pp. 17-28 ◽  
Author(s):  
Kousaku Ohno ◽  
Atsuyuki Okuda ◽  
Masumi Ohtsu ◽  
Genki Kimura
Keyword(s):  
Genetic Analysis ◽  
Cell Line ◽  
Temperature Sensitive ◽  
Cells In Culture ◽  
Isolation And Characterization ◽  
Fibroblastic Cells

Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation

1988 ◽  
Vol 8 (6) ◽  
pp. 2335-2341
Author(s):  
R J Akhurst ◽  
N B Flavin ◽  
J Worden
Keyword(s):  
Cell Line ◽  
Progenitor Cell ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Isolation And Characterization ◽  
New Variant ◽  
Myoblast Cell Line ◽  
Myoblast Cell ◽  
Progenitor Cell Line

A new variant rat myogenic cell line, ts485, was isolated by subcloning the cell line ts3b2 (H. T. Nguyen, R. M. Medford, and B. Nadal-Ginard, Cell 34:281-293, 1983). Unlike the progenitor cell line, ts485 was thermosensitive for differentiation. Experiments with conditioned medium suggested that diffusible extracellular factors were not involved in dictating the differential phenotypes of ts485 cells cultured at the permissive and nonpermissive temperatures. Temperature shift experiments performed on cultures of ts485 cells indicated that the temperature-sensitive lesion was in a factor active during the growth phase and required to trigger a cascade of events leading to terminal differentiation.

scholarly journals Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation.

1988 ◽  
Vol 8 (6) ◽  
pp. 2335-2341 ◽  
Author(s):  
R J Akhurst ◽  
N B Flavin ◽  
J Worden
Keyword(s):  
Cell Line ◽  
Progenitor Cell ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Isolation And Characterization ◽  
New Variant ◽  
Myoblast Cell Line ◽  
Myoblast Cell ◽  
Progenitor Cell Line

A new variant rat myogenic cell line, ts485, was isolated by subcloning the cell line ts3b2 (H. T. Nguyen, R. M. Medford, and B. Nadal-Ginard, Cell 34:281-293, 1983). Unlike the progenitor cell line, ts485 was thermosensitive for differentiation. Experiments with conditioned medium suggested that diffusible extracellular factors were not involved in dictating the differential phenotypes of ts485 cells cultured at the permissive and nonpermissive temperatures. Temperature shift experiments performed on cultures of ts485 cells indicated that the temperature-sensitive lesion was in a factor active during the growth phase and required to trigger a cascade of events leading to terminal differentiation.

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