scholarly journals Development of an oxygen-resistant hydrogen bacterium during plate cultivation under high oxygen tension.

1984 ◽  
Vol 48 (1) ◽  
pp. 165-170 ◽  
Author(s):  
Yoshihiro NAKAMURA ◽  
Jun-ichiro SOMEYA ◽  
Jiro OOYAMA
Keyword(s):  
Oxygen Tension ◽  
High Oxygen ◽  
Hydrogen Bacterium

scholarly journals Adaptation of oxygen-sensitive hydrogen bacterium N34 to high oxygen tension.

1979 ◽  
Vol 43 (9) ◽  
pp. 1825-1831
Author(s):  
Yoshihiro NAKAMURA ◽  
Jun-ichiro SOMEYA ◽  
Jiro OOYAMA
Keyword(s):  
Oxygen Tension ◽  
High Oxygen ◽  
Hydrogen Bacterium ◽  
Oxygen Sensitive

scholarly journals Adaptation of Oxygen-Sensitive Hydrogen Bacterium N34 to High Oxygen Tension

1979 ◽  
Vol 43 (9) ◽  
pp. 1825-1831
Author(s):  
Yoshihiro Nakamura ◽  
Jun-ichiro Someya ◽  
Jiro Ooyama
Keyword(s):  
Oxygen Tension ◽  
High Oxygen ◽  
Hydrogen Bacterium ◽  
Oxygen Sensitive

Monitoring preantral follicle survival and growth in bovine ovarian biopsies by repeated use of neutral red and cultured in vitro under low and high oxygen tension

2014 ◽  
Vol 82 (3) ◽  
pp. 387-395 ◽  
Author(s):  
Ellen P.A. Jorssen ◽  
An Langbeen ◽  
Erik Fransen ◽  
Emilia L. Martinez ◽  
Jo L.M.R. Leroy ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Neutral Red ◽  
High Oxygen ◽  
Preantral Follicle ◽  
Repeated Use ◽  
Survival And Growth

Increased Metallothionein Content in Rat Liver Induced by X Irradiation and Exposure to High Oxygen Tension

1983 ◽  
Vol 95 (2) ◽  
pp. 298 ◽  
Author(s):  
Noriyuki Shiraishi ◽  
Kaname Aono ◽  
Kozo Utsumi
Keyword(s):  
Oxygen Tension ◽  
Rat Liver ◽  
High Oxygen ◽  
X Irradiation

Fine Temporal Mapping of Plasmodium Falciparum growth Inhibition in Hypoxic Sickle Cell Trait Erythrocytes

Blood ◽  
2017 ◽  
Vol 130 (Suppl_1) ◽  
pp. 924-924
Author(s):  
Natasha Archer ◽  
Nicole Petersen ◽  
Martha Clark ◽  
Manoj Duraisingh
Keyword(s):  
Flow Cytometry ◽  
Carbon Monoxide ◽  
Dna Replication ◽  
Oxygen Tension ◽  
Sickle Cell ◽  
Sickle Cell Trait ◽  
High Oxygen ◽  
Low Oxygen ◽  
Trophozoite Stage ◽  
Sickle Hemoglobin

Abstract Background: Despite the global acceptance of Haldane's hypothesis that individuals with sickle cell trait (AS) are protected against malaria, an exact mechanism of resistance remains unknown. Several theories to explain the resistance such as increased splenic destruction, impaired hemoglobin digestion, reduced cytoadherence and specific translocation of host specific microRNAs have been proposed. Here we carefully examine the influence of hypoxia and its relation to parasite cytoadherence and growth in AS and normal (AA) human red blood cells (RBCs). Materials and Methods: To assess the influence of oxygen tension on parasite proliferation in AS RBCs, we followed the proliferation of tightly synchronized P. falciparum within AS and AA RBCs at 1, 5, 10, and 17% oxygen by flow cytometry and microscopy. Carbon monoxide (CO) studies were performed by introducing 100% CO gas into our enclosed chamber system every 21 hours to prevent sickling at low oxygen. Results: Flow cytometry assessment of DNA content confirms the absence of DNA replication within parasites in AS erythrocytes at low oxygen, however normal DNA replication is observed in the presence of high oxygen tension and when CO is introduced at low oxygen. Moving parasites from high to low oxygen tension greatly influences the extent of parasite maturity; parasites moved at 16 and 20 hours post invasion (hpi) do not mature beyond that of the late ring/early trophozoite stage while parasites moved at 24, 28, and 32 hpi develop increasingly more merozoites. Conclusion: Here, we show that in hypoxic conditions parasites in AS erythrocytes stall at the late ring/early trophozoite stage and do not replicate their DNA. We also show that treatment of AS erythrocytes with high oxygen tension or carbon monoxide, a potent inhibitor of sickle hemoglobin polymerization, reverses this phenotype. We propose that the mechanism of AS protection is largely due to P.falciparum's inability to digest polymerized sickle hemoglobin once the parasite sequesters in the hypoxic microvasculature. This is likely a key driver of the reduced parasite densities observed in actual infections in AS individuals. Figure 1 Figure 1. Disclosures No relevant conflicts of interest to declare.

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