scholarly journals Properties of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in villous and crypt cells of the rat small intestine.

1978 ◽  
Vol 42 (11) ◽  
pp. 2009-2014 ◽  
Author(s):  
Michihiro SUGANO ◽  
Hiroshi OKAMATSU ◽  
Takashi IDE
1980 ◽  
Vol 110 (2) ◽  
pp. 360-370 ◽  
Author(s):  
Michihiro Sugano ◽  
Takashi Ide ◽  
Hiroshi Okamatsu ◽  
Yasuhiko Fujisaki ◽  
Hitoshi Takahara

Metabolism ◽  
1994 ◽  
Vol 43 (4) ◽  
pp. 450-454 ◽  
Author(s):  
Kenneth R. Feingold ◽  
Dana E. Wilson ◽  
Ladonna C. Wood ◽  
Linda K. Kwong ◽  
Arthur H. Moser ◽  
...  

1978 ◽  
Vol 75 (11) ◽  
pp. 5548-5552 ◽  
Author(s):  
A. Quaroni ◽  
K. J. Isselbacher ◽  
E. Ruoslahti

1971 ◽  
Vol 49 (1) ◽  
pp. 38-43 ◽  
Author(s):  
M. W. Liebbrman ◽  
R. J. Sullivan ◽  
K. H. Shull ◽  
H. Liang ◽  
E. Farber

We have partially characterized and localized two previously reported deoxyribonucleases from the rat small intestine. After separation of the crypt cells and muscle (the deep layer) from the villus cells (the superficial layer), the latter was found to contain a deoxyribonuclease I with a pH optimum around 6, and a molecular weight of 32 000 – 35 000. It was activated by Co2+, Mg2+, and Mn2+. Ligation of the pancreatic duct reduced the activity in intestinal extracts to about one-third of control levels. A deoxyribonuclease II with a pH optimum of 5.3–5.4 was found associated with the continuously dividing intestinal crypt cells. It was inhibited by Mg2+ and activated by EDTA. Ligation of the pancreatic duct was without effect on this enzyme. The deoxyribonuclease I is probably largely extracellular and serves a digestive function while the deoxyribonuclease II probably is related to intracellular DNA metabolism.


1982 ◽  
Vol 112 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Michihiro Sugano ◽  
Yasuhiko Fujisaki ◽  
Hirosuke Oku ◽  
Takashi Ide

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