scholarly journals The Effect of Acetic Acid on the Association and the State of the Tyrosine Residue of an Active Fragment of Bovine Growth Hormone

1973 ◽  
Vol 37 (11) ◽  
pp. 2631-2637 ◽  
Author(s):  
Nobuyuki YAMASAKI ◽  
Kenji KANGAWA
Keyword(s):  
Growth Hormone ◽  
Acetic Acid ◽  
The State ◽  
Bovine Growth Hormone ◽  
Tyrosine Residue ◽  
Active Fragment

scholarly journals Amino Acid Sequence of a Biologically Active Fragment of Bovine Growth Hormone

1972 ◽  
Vol 247 (12) ◽  
pp. 3874-3880
Author(s):  
Nobuyuki Yamasaki ◽  
Kenji Kangawa ◽  
Shigeru Kobayashi ◽  
Motosuke Kikutani ◽  
Martin Sonenberg
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Biologically Active ◽  
Bovine Growth Hormone ◽  
Active Fragment

Effects of an S84E Mutation of Bovine Growth Hormone in Transgenic Mice

2006 ◽  
Vol 231 (3) ◽  
pp. 296-302 ◽  
Author(s):  
E. J. H. Schenck ◽  
C. L. Brooks
Keyword(s):  
Growth Hormone ◽  
Acetic Acid ◽  
Transgenic Mice ◽  
Glutamic Acid ◽  
Receptor Binding ◽  
Growth Hormones ◽  
Side Chain ◽  
Bovine Growth Hormone ◽  
Wild Type ◽  
Transgenic Strains

The ability of mutant bovine growth hormones (bGH) to serve as either agonist or antagonist has been demonstrated in transgenic mice. We have prepared two transgenic strains of FVB/N mice, one expressing wild-type bGH and a second with a glutamic acid mutation at serine 84 in helix 2. Comparison of their phenotypes to those of nontransgenic littermates indicates that wild-type bGH induces a previously described phenotype for hyper-somatotrophic mice. In contrast, the replacement of the side chain hydroxyl at serine 84 with acetic acid produced a phenotype that expressed bGH at appreciable concentrations, but failed to elicit the phenotype observed with either an agonist or an antagonist of bGH. These results indicate that serine 84 is crucial for the activity of bGH despite this site being distal to the receptor binding surfaces.

ANALYSIS OF THE BIPHASIC ACTION OF GROWTH HORMONE IN VITRO ON THE RAT DIAPHRAGM

1968 ◽  
Vol 57 (3_Suppl) ◽  
pp. S19-S35 ◽  
Author(s):  
Å. Hjalmarson
Keyword(s):  
Growth Hormone ◽  
Actinomycin D ◽  
Accumulation Rate ◽  
Inhibitory Effect ◽  
Bovine Growth Hormone ◽  
Rat Diaphragm ◽  
Dual Nature ◽  
Hypophysectomized Rats ◽  
D 20

ABSTRACT In vitro addition of bovine growth hormone (GH) to intact hemidiaphragms from hypophysectomized rats has previously been found to produce both an early stimulatory effect lasting for 2—3 hours and a subsequent late inhibitory effect during which the muscle is insensitive to further addition of GH (Hjalmarson 1968). These effects on the accumulation rate of α-aminoisobutyric acid (AIB) and D-xylose have been further studied. In presence of actinomycin D (20 μg/ml) or puromycin (100 μg/ml) the duration of the stimulatory effect of GH (25 μg/ml) was prolonged to last for at least 4—5 hours and the late inhibitory effect was prevented. Similar results were obtained when glucose-free incubation medium was used. Preincubation of the diaphragm at different glucose concentrations (0—5 mg/ml) for 3 hours did not change the GH sensitivity. Addition of insulin at start of incubation could not prevent GH from inducing its late inhibitory effect, while dexamethasone seemed to potentiate this effect of GH. Furthermore, adrenaline was found to decrease the uptake of AIB-14C and D-xylose-14C in the diaphragm, but not to change the sensitivity of the muscle to GH. Preincubation of the diaphragm for 3 hours with puromycin in a concentration of 200 μg/ml markedly decreased the subsequent basal uptake of both AIB-14C and D-xylose-14C, in the presence of puromycin, and abolished the stimulatory effect of GH on the accumulation of AIB-14C. However, the effect of GH on the accumulation of D-xylose-14C was unchanged. The present observations are discussed and evaluated in relation to various mechanisms of GH action proposed to explain the dual nature of the hormone.

Growth hormone activity in man with components of tryptic digests of bovine growth hormone

Metabolism ◽  
1967 ◽  
Vol 16 (9) ◽  
pp. 830-845 ◽  
Author(s):  
A.C. Nadler ◽  
M. Sonenberg ◽  
M.I. New ◽  
C.A. Free
Keyword(s):  
Growth Hormone ◽  
Bovine Growth Hormone ◽  
Hormone Activity

scholarly journals Association Between SSCP Haplotypes at the Bovine Growth Hormone Gene and Milk Protein Percentage

Genetics ◽  
1996 ◽  
Vol 142 (3) ◽  
pp. 945-951
Author(s):  
A Lagziel ◽  
E Lipkin ◽  
M Soller
Keyword(s):  
Growth Hormone ◽  
Candidate Gene ◽  
Milk Protein ◽  
Haplotype Analysis ◽  
Bos Taurus ◽  
Bovine Growth Hormone ◽  
Growth Hormone Gene ◽  
Protein Percentage ◽  
Quantitative Genetic ◽  
Positive Effect

Abstract The bovine Growth Hormone gene (bGH) is an attractive candidate gene for milk production in cattle. Single-strand conformation polymorphisms at bGH were identified and used to define haplotype configurations at this gene in the Israeli Holstein dairy cattle population (Bos taurus) and in the parent animals of the International Bovine Reference Family Panel (a collection of B. taurus and B. indicus crosses). B. taurus and B. indicus haplotypes at the bGH gene differed qualitatively, confirming the previously proposed long evolutionary separation of these cattle subraces. Only a small number of bGH haplotypes were present in the Israel Holstein population. One of the haplotypes, apparently of B. indicus origin, was found to have a highly significant positive effect on milk protein percentage. This illustrates the utility of the haplotype approach for uncovering candidate gene involvement in quantitative genetic variation in agricultural populations. The strong effect of an indicine haplotype in a taurine background raises the possibility that indicine alleles at other candidate genes may comprise a genetic resource for improvement of taurine populations. It is proposed that haplotype analysis may be a useful adjunct to measures of genetic distance for evaluating rare breeds with respect to gene conservation.

scholarly journals Sequence Variations in the Bovine Growth Hormone Gene Characterized by Single-Strand Conformation Polymorphism (SSCP) Analysis and Their Association with Milk Production Traits in Holsteins

Genetics ◽  
1996 ◽  
Vol 144 (4) ◽  
pp. 1809-1816 ◽  
Author(s):  
Jianbo Yao ◽  
Samuel E Aggrey ◽  
David Zadworny ◽  
J Flan Hayes ◽  
Urs Kühnlein
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Yield ◽  
Single Strand Conformation Polymorphism ◽  
Sscp Analysis ◽  
Bovine Growth Hormone ◽  
Production Traits ◽  
Milk Production Traits ◽  
Sequence Variations ◽  
Conformation Polymorphism

Sequence variations in the bovine growth hormone (GH) gene were investigated by single strand conformation polymorphism (SSCP) analysis of seven amplified fragments covering almost the entire gene (2.7 kb). SSCPs were detected in four of these fragments and a total of six polymorphisms were found in a sample of 128 Holstein bulls. Two polymorphisms, a T→C transition in the third intron (designated GH4.1) and an A→C transversion in the fifth exon (designated GH6.2), were shown to be associated with milk production traits. GH4.1c/GH4.1c bulls had higher milk yield than GH4.1c/GH4.1t (P ≤ 0.005) and GH4.1t/GH4.1t (P ≤ 0.0022) bulls. GH4.1c/GH4.1c bulls had higher kg fat (P ≤ 0.0076) and protein (P ≤ 0.0018) than GH4.1c/GH4.1t bulls. Similar effects on milk production traits with the GH6.2 polymorphism were observed with the GH6.2a allele being the favorable allele. The average effects of the gene substitution for GH4.1 and GH6.2 are similar, with ±300 kg for milk yield, ±8 kg for fat content and ±7 kg for protein content per lactation. The positive association of GH4.1c and GH6.2a with milk production traits may be useful for improving milk performance in dairy cattle.

Sign in / Sign up

Export Citation Format

Share Document