Development of a Homologous Transformation System forAspergillus aculeatusBased on thesCGene Encoding ATP-Sulfurylase

2009 ◽  
Vol 73 (5) ◽  
pp. 1197-1199 ◽  
Author(s):  
Hiromi ADACHI ◽  
Shuji TANI ◽  
Shin KANAMASA ◽  
Jun-ichi SUMITANI ◽  
Takashi KAWAGUCHI
Keyword(s):  
Transformation System ◽  
Atp Sulfurylase ◽  
Homologous Transformation

Development of a homologous transformation system for Aspergillus niger based on the pyrG gene

1987 ◽  
Vol 206 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Wim van Hartingsveldt ◽  
Ineke E. Mattern ◽  
Cora M. J. van Zeijl ◽  
Peter H. Pouwels ◽  
Cees A. M. J. J. van den Hondel
Keyword(s):  
Aspergillus Niger ◽  
Transformation System ◽  
Homologous Transformation

Deletion Analysis of the Taka-amylase A Gene Promoter Using a Homologous Transformation System inAspergillus oryzae

1992 ◽  
Vol 56 (11) ◽  
pp. 1849-1853 ◽  
Author(s):  
Kozo Tsuchiya ◽  
Setsuzo Tada ◽  
Katsuya Gomi ◽  
Katsuhiko Kitamoto ◽  
Chieko Kumagai ◽  
...  
Keyword(s):  
Gene Promoter ◽  
Deletion Analysis ◽  
Transformation System ◽  
Homologous Transformation

The nia gene of Fusarium oxysporum: isolation, sequence and development of a homologous transformation system

Gene ◽  
1993 ◽  
Vol 131 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Annick Diolez ◽  
Thierry Langin ◽  
Catherine Gerlinger ◽  
Yves Brygoo ◽  
Marie-Josée Daboussi
Keyword(s):  
Fusarium Oxysporum ◽  
Transformation System ◽  
Homologous Transformation

scholarly journals Characterization, regulation, and phylogenetic analyses of thePenicillium griseoroseumnitrate reductase gene and its use as selection marker for homologous transformation

2004 ◽  
Vol 50 (11) ◽  
pp. 891-900 ◽  
Author(s):  
Jorge Fernando Pereira ◽  
Marisa Vieira de Queiroz ◽  
Francis Júlio Fagundes Lopes ◽  
Rodrigo Barros Rocha ◽  
Marie-Josée Daboussi ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Metabolism ◽  
Phylogenetic Analyses ◽  
Regulatory Region ◽  
Selection Marker ◽  
Pcr Analysis ◽  
Transformation System ◽  
Homologous Transformation ◽  
Reductase Gene ◽  
Penicillium Griseoroseum

Penicillium griseoroseum has been studied because of its efficient pectinases production. In this work, the Penicillium griseoroseum nitrate reductase gene was characterized, transcriptionaly analyzed in different nitrogen sources, and used to create a phylogenetic tree and to develop a homologous transformation system. The regulatory region contained consensus signals involved in nitrogen metabolism and the structural region was possibly interrupted by 6 introns coding for a deduced protein with 864 amino acids. RT–PCR analysis revealed high amounts of niaD transcript in the presence of nitrate. Transcription was repressed by ammonium, urea, and glutamine showing an efficient turnover of the niaD mRNA. Phylogenetics analysis showed distinct groups clearly separated in accordance with the classical taxonomy. A mutant with a 122-bp deletion was used in homologous transformation experiments and showed a transformation frequency of 14 transformants/µg DNA. All analyzed transformants showed that both single- and double-crossover recombination occurred at the niaD locus. The establishment of this homologous transformation system is an essential step for the improvement of pectinase production in Penicillium griseoroseum.Key words: nitrate reductase, nitrogen metabolism, Penicillium griseoroseum, phylogenetic analysis, homologous transformation.

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