Development of a homologous transformation system for Aspergillus parasiticus with the gene encoding nitrate reductase

1990 ◽  
Vol 224 (2) ◽  
pp. 294-296 ◽  
Author(s):  
Jason S. Horng ◽  
Perng-Kuang Chang ◽  
James J. Pestka ◽  
John E. Linz
Keyword(s):  
Nitrate Reductase ◽  
Aspergillus Parasiticus ◽  
Transformation System ◽  
Gene Encoding ◽  
Homologous Transformation

scholarly journals Characterization, regulation, and phylogenetic analyses of thePenicillium griseoroseumnitrate reductase gene and its use as selection marker for homologous transformation

2004 ◽  
Vol 50 (11) ◽  
pp. 891-900 ◽  
Author(s):  
Jorge Fernando Pereira ◽  
Marisa Vieira de Queiroz ◽  
Francis Júlio Fagundes Lopes ◽  
Rodrigo Barros Rocha ◽  
Marie-Josée Daboussi ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Metabolism ◽  
Phylogenetic Analyses ◽  
Regulatory Region ◽  
Selection Marker ◽  
Pcr Analysis ◽  
Transformation System ◽  
Homologous Transformation ◽  
Reductase Gene ◽  
Penicillium Griseoroseum

Penicillium griseoroseum has been studied because of its efficient pectinases production. In this work, the Penicillium griseoroseum nitrate reductase gene was characterized, transcriptionaly analyzed in different nitrogen sources, and used to create a phylogenetic tree and to develop a homologous transformation system. The regulatory region contained consensus signals involved in nitrogen metabolism and the structural region was possibly interrupted by 6 introns coding for a deduced protein with 864 amino acids. RT–PCR analysis revealed high amounts of niaD transcript in the presence of nitrate. Transcription was repressed by ammonium, urea, and glutamine showing an efficient turnover of the niaD mRNA. Phylogenetics analysis showed distinct groups clearly separated in accordance with the classical taxonomy. A mutant with a 122-bp deletion was used in homologous transformation experiments and showed a transformation frequency of 14 transformants/µg DNA. All analyzed transformants showed that both single- and double-crossover recombination occurred at the niaD locus. The establishment of this homologous transformation system is an essential step for the improvement of pectinase production in Penicillium griseoroseum.Key words: nitrate reductase, nitrogen metabolism, Penicillium griseoroseum, phylogenetic analysis, homologous transformation.

Development of a Homologous Transformation System forAspergillus aculeatusBased on thesCGene Encoding ATP-Sulfurylase

2009 ◽  
Vol 73 (5) ◽  
pp. 1197-1199 ◽  
Author(s):  
Hiromi ADACHI ◽  
Shuji TANI ◽  
Shin KANAMASA ◽  
Jun-ichi SUMITANI ◽  
Takashi KAWAGUCHI
Keyword(s):  
Transformation System ◽  
Atp Sulfurylase ◽  
Homologous Transformation

Synthesis and bacterial expression of a gene encoding the heme domain of assimilatory nitrate reductase

2002 ◽  
Vol 402 (1) ◽  
pp. 38-50 ◽  
Author(s):  
Michael J. Barber ◽  
Shawn K. Desai ◽  
Christopher C. Marohnic ◽  
Hector H. Hernandez ◽  
Veronica V. Pollock
Keyword(s):  
Nitrate Reductase ◽  
Bacterial Expression ◽  
Gene Encoding ◽  
Heme Domain

scholarly journals Respiratory Nitrate Ammonification by Shewanella oneidensis MR-1

2006 ◽  
Vol 189 (2) ◽  
pp. 656-662 ◽  
Author(s):  
Claribel Cruz-García ◽  
Alison E. Murray ◽  
Joel A. Klappenbach ◽  
Valley Stewart ◽  
James M. Tiedje
Keyword(s):  
Nitrous Oxide ◽  
Nitrate Reductase ◽  
Electron Acceptor ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Shewanella Oneidensis ◽  
Gene Encoding ◽  
Nitrate Ammonification ◽  
Sequential Reduction

ABSTRACT Anaerobic cultures of Shewanella oneidensis MR-1 grown with nitrate as the sole electron acceptor exhibited sequential reduction of nitrate to nitrite and then to ammonium. Little dinitrogen and nitrous oxide were detected, and no growth occurred on nitrous oxide. A mutant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enzyme is the sole nitrate reductase. Hence, S. oneidensis MR-1 conducts respiratory nitrate ammonification, also termed dissimilatory nitrate reduction to ammonium, but not respiratory denitrification.

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